BacMap

Definition	Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession	NC_012563
Length	4,155,278

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The map label for this gene is yhcG [H]

Identifier: 226948473

GI number: 226948473

Start: 1424112

End: 1424984

Strand: Direct

Name: yhcG [H]

Synonym: CLM_1360

Alternate gene names: 226948473

Gene position: 1424112-1424984 (Clockwise)

Preceding gene: 226948472

Following gene: 226948474

Centisome position: 34.27

GC content: 26.35

Gene sequence:

>873_bases
ATGAGTAATATAGTAATAAAAAATGTTACTAAATCATACCAAAGAAAAGAAGCGTTATCAAATATTTCCATTAACATAGA
AGAAGGAAAAATATATGGTCTTCTTGGAAGAAATGGAGCAGGAAAGACAACTCTGCTGCATCTTATAACTAATAGAGCCA
TTTTAGATAGTGGAGAAATCTTAATAGATGGTGAAAATGTTTATGAAAATAACAAGGCCCTTTCTAAAGTTTATTTTATG
GAAGAAAAAAATCTTTTTCCTGAATCAATGAAAATAGTAGATGTTTTTGAATGGACAAAAGAGTTTTATGAAGACTTTGA
TATGGAATATGCTATTAAACTTAGTGAGCTTTTTAAATTAAATATAAAGAAAAAAGTAAAAAATTTATCAACAGGATATA
AATCAATAAGTAAAATTATTGCTACTTTAGCTTCAGGAGCAGAAATTTTAATTTTTGATGAACCTATTTTAGGCTTAGAT
GCTAACCATAGAGAACTTTTTTATAAAGAGCTATTAAAGATTTATGGAGAAGGGCAAAAAACAATTGTAATATCCACCCA
CATAATAGAGGAAATATCTCATCTTTTAGAAAAAGTAGTTATATTAAAGGAAGGTAAAATAATAGAAAGTGATTCTGTTG
AAAATATGCTTAATTTAGCCTATGCAGTTTCAGGTAGTAGTGATGATGTAGATAAATATATAACCGCTAAAAACACAATA
GATATAGAAACTTTAGGCTCATATAAAAAAGCTACAATTTTACAAGAAAGAACTAAAGAAGATTTAGATAATGTAAAGAA
ACTAGAATTAGAAATTAGCACAGTAGAACTTCAAAAACTATTTATATATTTAACAAATATGGAGGAAAAATAG

Upstream 100 bases:

>100_bases
AAATTTTTTGATAGCTACATTTTAAGTCTTGTGGATGAAGCTAAAAGACTAGGAATAACTATAGATGAAATAATGGATAT
GATAAAAGGGGGATATGGCA

Downstream 100 bases:

>100_bases
ATGGAAAAAAGTAATAAAATTTGCAAATATCAATTAAAGACTTCTATAAAACCTATATTGATTTATTATAGTATATTAAT
AGGATTCTTATTATTAATAT

Product: ABC transporter ATP-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEILIDGENVYENNKALSKVYFM
EEKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSELFKLNIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLD
ANHRELFYKELLKIYGEGQKTIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTI
DIETLGSYKKATILQERTKEDLDNVKKLELEISTVELQKLFIYLTNMEEK

Sequences:

>Translated_290_residues
MSNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEILIDGENVYENNKALSKVYFM
EEKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSELFKLNIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLD
ANHRELFYKELLKIYGEGQKTIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTI
DIETLGSYKKATILQERTKEDLDNVKKLELEISTVELQKLFIYLTNMEEK
>Mature_289_residues
SNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEILIDGENVYENNKALSKVYFME
EKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSELFKLNIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLDA
NHRELFYKELLKIYGEGQKTIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTID
IETLGSYKKATILQERTKEDLDNVKKLELEISTVELQKLFIYLTNMEEK

Specific function: Part Of The Abc Transporter Complex Cysawtp Involved In Sulfate/Thiosulfate Import. Responsible For Energy Coupling To The Transport System. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=214, Percent_Identity=28.9719626168224, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI30795238, Length=214, Percent_Identity=28.9719626168224, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI27477115, Length=214, Percent_Identity=24.2990654205607, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI21536376, Length=240, Percent_Identity=27.0833333333333, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI31657092, Length=222, Percent_Identity=27.4774774774775, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI45446740, Length=212, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI47078218, Length=212, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI27436953, Length=218, Percent_Identity=23.8532110091743, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI105990541, Length=212, Percent_Identity=25, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI116734710, Length=226, Percent_Identity=26.9911504424779, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI153792144, Length=221, Percent_Identity=23.9819004524887, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI6005701, Length=209, Percent_Identity=24.4019138755981, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI27262624, Length=241, Percent_Identity=21.1618257261411, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI27262626, Length=241, Percent_Identity=21.1618257261411, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI9955968, Length=229, Percent_Identity=25.764192139738, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI9955966, Length=229, Percent_Identity=25.764192139738, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI45243524, Length=229, Percent_Identity=25.764192139738, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1788761, Length=223, Percent_Identity=30.4932735426009, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI87081791, Length=210, Percent_Identity=28.0952380952381, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788450, Length=232, Percent_Identity=27.1551724137931, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48995001, Length=216, Percent_Identity=26.3888888888889, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790739, Length=226, Percent_Identity=23.0088495575221, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790190, Length=223, Percent_Identity=26.457399103139, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788210, Length=295, Percent_Identity=23.728813559322, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994943, Length=227, Percent_Identity=22.9074889867841, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786563, Length=218, Percent_Identity=27.9816513761468, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081782, Length=219, Percent_Identity=24.6575342465753, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788225, Length=235, Percent_Identity=24.6808510638298, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787370, Length=212, Percent_Identity=25, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081709, Length=231, Percent_Identity=24.2424242424242, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787029, Length=219, Percent_Identity=27.8538812785388, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789586, Length=234, Percent_Identity=24.3589743589744, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787712, Length=217, Percent_Identity=27.6497695852535, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787792, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786319, Length=213, Percent_Identity=26.7605633802817, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790467, Length=227, Percent_Identity=24.6696035242291, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788472, Length=232, Percent_Identity=25, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790162, Length=261, Percent_Identity=25.2873563218391, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786345, Length=229, Percent_Identity=24.0174672489083, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789891, Length=216, Percent_Identity=24.0740740740741, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788540, Length=191, Percent_Identity=29.8429319371728, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789672, Length=225, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789593, Length=228, Percent_Identity=24.1228070175439, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790525, Length=237, Percent_Identity=23.6286919831224, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082268, Length=221, Percent_Identity=24.8868778280543, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787143, Length=228, Percent_Identity=25.8771929824561, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=22.5806451612903, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082267, Length=214, Percent_Identity=21.9626168224299, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787112, Length=231, Percent_Identity=26.8398268398268, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=228, Percent_Identity=28.0701754385965, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=221, Percent_Identity=29.8642533936652, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193211017, Length=221, Percent_Identity=29.8642533936652, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71996809, Length=221, Percent_Identity=29.8642533936652, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=224, Percent_Identity=25.8928571428571, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17552010, Length=260, Percent_Identity=25.7692307692308, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=201, Percent_Identity=26.3681592039801, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI193208177, Length=269, Percent_Identity=24.5353159851301, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=222, Percent_Identity=27.4774774774775, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=222, Percent_Identity=27.4774774774775, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71983516, Length=222, Percent_Identity=27.4774774774775, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=214, Percent_Identity=29.9065420560748, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322177, Length=202, Percent_Identity=26.7326732673267, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324585, Length=204, Percent_Identity=29.9019607843137, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281362751, Length=241, Percent_Identity=29.4605809128631, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24650853, Length=235, Percent_Identity=29.3617021276596, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24650855, Length=237, Percent_Identity=29.957805907173, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24582014, Length=227, Percent_Identity=28.1938325991189, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582012, Length=227, Percent_Identity=28.1938325991189, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI85725262, Length=266, Percent_Identity=28.9473684210526, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI116007184, Length=224, Percent_Identity=27.6785714285714, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221500365, Length=224, Percent_Identity=27.6785714285714, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24666092, Length=223, Percent_Identity=31.390134529148, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI221512771, Length=211, Percent_Identity=30.8056872037915, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116007328, Length=215, Percent_Identity=30.2325581395349, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574150, Length=215, Percent_Identity=30.2325581395349, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24643674, Length=223, Percent_Identity=26.9058295964126, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581407, Length=229, Percent_Identity=25.3275109170306, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571209, Length=213, Percent_Identity=24.8826291079812, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28573571, Length=212, Percent_Identity=25.9433962264151, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573573, Length=211, Percent_Identity=26.0663507109005, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 33038; Mature: 32906

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEI
CCCEEEHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCHHHHHHHHHCCEEECCCCE
LIDGENVYENNKALSKVYFMEEKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSELFKL
EECCCCCCCCCHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHH
NIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLDANHRELFYKELLKIYGEGQK
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCE
TIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTI
EEEEEHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHEEECCCCCCHHHHEECCCCE
DIETLGSYKKATILQERTKEDLDNVKKLELEISTVELQKLFIYLTNMEEK
EHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHEEHHEEEECCCCC
>Mature Secondary Structure 
SNIVIKNVTKSYQRKEALSNISINIEEGKIYGLLGRNGAGKTTLLHLITNRAILDSGEI
CCEEEHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCHHHHHHHHHCCEEECCCCE
LIDGENVYENNKALSKVYFMEEKNLFPESMKIVDVFEWTKEFYEDFDMEYAIKLSELFKL
EECCCCCCCCCHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHH
NIKKKVKNLSTGYKSISKIIATLASGAEILIFDEPILGLDANHRELFYKELLKIYGEGQK
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCE
TIVISTHIIEEISHLLEKVVILKEGKIIESDSVENMLNLAYAVSGSSDDVDKYITAKNTI
EEEEEHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHEEECCCCCCHHHHEECCCCE
DIETLGSYKKATILQERTKEDLDNVKKLELEISTVELQKLFIYLTNMEEK
EHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHEEHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]