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The map label for this gene is ilvD [H]

Identifier: 226947515

GI number: 226947515

Start: 335306

End: 337021

Strand: Direct

Name: ilvD [H]

Synonym: CLM_0349

Alternate gene names: 226947515

Gene position: 335306-337021 (Clockwise)

Preceding gene: 226947514

Following gene: 226947516

Centisome position: 8.07

GC content: 35.08

Gene sequence:

>1716_bases
ATGATTAGTCAAGATATTAGAAAGATTGCTCCAGAAATGGATCCACTTCGTAGGGGTATGGGATGGTCAGTTGAAGATTT
ATCTAAGCCTCAAATTATAGTTGAAAGTACCTTTGGAGATAGTCACCCAGGAAGTGCTCATTTAATGAAATTTGCAAATA
ATGCGGTACAGGGTGTAGCAGAAAGAGGAGGAAAGGCTGCAAGATATTTTGCAACAGATATATGTGATGGTATGGCTCAA
GGTCACGATGGAATCAATTATTCATTAGCATCTAGGGATACTATAACATCACTTATAGAAATTCATGCGAATGCCACTCC
TTTTGATGGAGGGGTATTTATATCAAGTTGTGATAAGGCTGTTCCATCTCACTTAATGGCTATAGGAAGACTTAATATAC
CATCAATTGTTGTTACAGGTGGCGTTATGGAAGCGGGACCTAACCTTTTAACTTTAGAGCAAATTGGTGCTTATAGTGCT
ATGTATCAACGAAAAGAAATTACAGAGGAAGAATTAACTTACTATAAACATAATGCATGTCCATCTTGTGGAGCTTGTTC
TTTTATGGGAACAGCTTCAACAATGCAGGTTATGGCAGAAGCCTTAGGATTAATGTTACCTGGTAGTGCTTTAATGCCCG
CAACTTGTAAAGACTTAGAAGATGTTGCAGTAGAGGCTGGGAAACAAGTTGTTGAACTAGCAAAGATGAATCTTAAGCCA
AAGGATATAGTTACAGAAAAATCCTTTGAAAATGCAATTATAATTCATGCAGCAATTTCGGGATCTACTAATTCATTATT
ACACATTCCAGCAATAGCTCATGAATTTGGTATAAAAATTGATGAAGAAAGTTTTGATAGAATACATCGATACGCTCCAT
ATCTTTTAAATATACGTCCAGCAGGAAAATGGCCGGCAGAATATTTTTATTATGCAGGCGGAGTACCAGCAATAATGGAA
GAAGTAAAGCATTTATTACATTTAGATGTAATGACTGTTACAGGTAAAACTTTAGATGAGAATTTAGAGGACTTAAAGAA
GAATGGATATTATAAGAATTGTGATAAATACCTTAAAAAGGTTGGACTTACAAGAAGAGATGTTATAAGACCAATAAGTG
AACCTATAGGTAAAAATGGAGCTATTGCAATTTTAAAAGGAAATATAGCGCCAGAAGGAGCAGTAGTTAAACATTCAGCC
GTACCAAAAGAAATGCATAAAGGAATTTTAAAGGCAAGACCTTTTGATAGTGAAGAAGAAGCAATATCAGCTATTATATC
TAAAAAAATAAATCCAGGAGATGCTGTATTTATAAGATATGAAGGCCCAAAAGGAAGTGGAATGCCTGAAATGTTTTATA
CAACAGAAGCAATATCCTCAGATAAAGAATTATCTGCAAGTATTGCACTTATTACAGATGGAAGATTCTCAGGGGCATCC
AAAGGGCCTGCAATAGGACATGTTTCTCCCGAAGCAGCAGTGGGAGGGCCAATTGCCTTAGTTGAAGAGAATGATTTAAT
TGAAATAGATATAGAGAAAAGAATACTACAATTAGTTGGTGTTAATGGACAAAAATTAAGTGAAGAAGCCATAGATAAGG
TTTTAGTAGAACGTAAAGCTAAGTGGGAAAAAAGAGAAAATAAATATAAATCAGGAATATTAAAGATATTTTCAGAAAGA
GCAGTTTCACCAATGAAGGGTGGATATATGGAATAA

Upstream 100 bases:

>100_bases
TATTTAGTAAAAGCTCGGAAAGTGGATATACCATGTTCCGAGCTATGCTTTAAACAAGATGCATATAAAGGGAATATTTA
TGTTAAATAAGGAGGTTAAT

Downstream 100 bases:

>100_bases
TAATATTTCTGTAAGAGTTAGTACAATAATAGATTTATGGAGGTTTTTATTGTGATTAAGATGAAGGTTTTAAATGCTCT
AAAGAGTTGTAAAATAGTAG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MISQDIRKIAPEMDPLRRGMGWSVEDLSKPQIIVESTFGDSHPGSAHLMKFANNAVQGVAERGGKAARYFATDICDGMAQ
GHDGINYSLASRDTITSLIEIHANATPFDGGVFISSCDKAVPSHLMAIGRLNIPSIVVTGGVMEAGPNLLTLEQIGAYSA
MYQRKEITEEELTYYKHNACPSCGACSFMGTASTMQVMAEALGLMLPGSALMPATCKDLEDVAVEAGKQVVELAKMNLKP
KDIVTEKSFENAIIIHAAISGSTNSLLHIPAIAHEFGIKIDEESFDRIHRYAPYLLNIRPAGKWPAEYFYYAGGVPAIME
EVKHLLHLDVMTVTGKTLDENLEDLKKNGYYKNCDKYLKKVGLTRRDVIRPISEPIGKNGAIAILKGNIAPEGAVVKHSA
VPKEMHKGILKARPFDSEEEAISAIISKKINPGDAVFIRYEGPKGSGMPEMFYTTEAISSDKELSASIALITDGRFSGAS
KGPAIGHVSPEAAVGGPIALVEENDLIEIDIEKRILQLVGVNGQKLSEEAIDKVLVERKAKWEKRENKYKSGILKIFSER
AVSPMKGGYME

Sequences:

>Translated_571_residues
MISQDIRKIAPEMDPLRRGMGWSVEDLSKPQIIVESTFGDSHPGSAHLMKFANNAVQGVAERGGKAARYFATDICDGMAQ
GHDGINYSLASRDTITSLIEIHANATPFDGGVFISSCDKAVPSHLMAIGRLNIPSIVVTGGVMEAGPNLLTLEQIGAYSA
MYQRKEITEEELTYYKHNACPSCGACSFMGTASTMQVMAEALGLMLPGSALMPATCKDLEDVAVEAGKQVVELAKMNLKP
KDIVTEKSFENAIIIHAAISGSTNSLLHIPAIAHEFGIKIDEESFDRIHRYAPYLLNIRPAGKWPAEYFYYAGGVPAIME
EVKHLLHLDVMTVTGKTLDENLEDLKKNGYYKNCDKYLKKVGLTRRDVIRPISEPIGKNGAIAILKGNIAPEGAVVKHSA
VPKEMHKGILKARPFDSEEEAISAIISKKINPGDAVFIRYEGPKGSGMPEMFYTTEAISSDKELSASIALITDGRFSGAS
KGPAIGHVSPEAAVGGPIALVEENDLIEIDIEKRILQLVGVNGQKLSEEAIDKVLVERKAKWEKRENKYKSGILKIFSER
AVSPMKGGYME
>Mature_571_residues
MISQDIRKIAPEMDPLRRGMGWSVEDLSKPQIIVESTFGDSHPGSAHLMKFANNAVQGVAERGGKAARYFATDICDGMAQ
GHDGINYSLASRDTITSLIEIHANATPFDGGVFISSCDKAVPSHLMAIGRLNIPSIVVTGGVMEAGPNLLTLEQIGAYSA
MYQRKEITEEELTYYKHNACPSCGACSFMGTASTMQVMAEALGLMLPGSALMPATCKDLEDVAVEAGKQVVELAKMNLKP
KDIVTEKSFENAIIIHAAISGSTNSLLHIPAIAHEFGIKIDEESFDRIHRYAPYLLNIRPAGKWPAEYFYYAGGVPAIME
EVKHLLHLDVMTVTGKTLDENLEDLKKNGYYKNCDKYLKKVGLTRRDVIRPISEPIGKNGAIAILKGNIAPEGAVVKHSA
VPKEMHKGILKARPFDSEEEAISAIISKKINPGDAVFIRYEGPKGSGMPEMFYTTEAISSDKELSASIALITDGRFSGAS
KGPAIGHVSPEAAVGGPIALVEENDLIEIDIEKRILQLVGVNGQKLSEEAIDKVLVERKAKWEKRENKYKSGILKIFSER
AVSPMKGGYME

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=588, Percent_Identity=36.5646258503401, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1786464, Length=563, Percent_Identity=34.9911190053286, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI2367371, Length=535, Percent_Identity=35.3271028037383, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI1788157, Length=460, Percent_Identity=31.7391304347826, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322476, Length=555, Percent_Identity=35.3153153153153, Blast_Score=305, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 62037; Mature: 62037

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00589 PTS_HPR_SER ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISQDIRKIAPEMDPLRRGMGWSVEDLSKPQIIVESTFGDSHPGSAHLMKFANNAVQGVA
CCCHHHHHHCCCCCHHHHCCCCCHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ERGGKAARYFATDICDGMAQGHDGINYSLASRDTITSLIEIHANATPFDGGVFISSCDKA
HCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCEEHHHHHHH
VPSHLMAIGRLNIPSIVVTGGVMEAGPNLLTLEQIGAYSAMYQRKEITEEELTYYKHNAC
HHHHHHHHCCCCCCEEEEECCHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
PSCGACSFMGTASTMQVMAEALGLMLPGSALMPATCKDLEDVAVEAGKQVVELAKMNLKP
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KDIVTEKSFENAIIIHAAISGSTNSLLHIPAIAHEFGIKIDEESFDRIHRYAPYLLNIRP
HHHHCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHCCEECHHHHHHHHHCCCEEEEEEC
AGKWPAEYFYYAGGVPAIMEEVKHLLHLDVMTVTGKTLDENLEDLKKNGYYKNCDKYLKK
CCCCCHHHEEECCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCHHHHHHHH
VGLTRRDVIRPISEPIGKNGAIAILKGNIAPEGAVVKHSAVPKEMHKGILKARPFDSEEE
HCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCCCHHH
AISAIISKKINPGDAVFIRYEGPKGSGMPEMFYTTEAISSDKELSASIALITDGRFSGAS
HHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHEEHHHHCCCCCCCEEEEEEECCCCCCCC
KGPAIGHVSPEAAVGGPIALVEENDLIEIDIEKRILQLVGVNGQKLSEEAIDKVLVERKA
CCCCCCCCCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KWEKRENKYKSGILKIFSERAVSPMKGGYME
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MISQDIRKIAPEMDPLRRGMGWSVEDLSKPQIIVESTFGDSHPGSAHLMKFANNAVQGVA
CCCHHHHHHCCCCCHHHHCCCCCHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ERGGKAARYFATDICDGMAQGHDGINYSLASRDTITSLIEIHANATPFDGGVFISSCDKA
HCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCEEHHHHHHH
VPSHLMAIGRLNIPSIVVTGGVMEAGPNLLTLEQIGAYSAMYQRKEITEEELTYYKHNAC
HHHHHHHHCCCCCCEEEEECCHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
PSCGACSFMGTASTMQVMAEALGLMLPGSALMPATCKDLEDVAVEAGKQVVELAKMNLKP
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KDIVTEKSFENAIIIHAAISGSTNSLLHIPAIAHEFGIKIDEESFDRIHRYAPYLLNIRP
HHHHCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHCCEECHHHHHHHHHCCCEEEEEEC
AGKWPAEYFYYAGGVPAIMEEVKHLLHLDVMTVTGKTLDENLEDLKKNGYYKNCDKYLKK
CCCCCHHHEEECCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCHHHHHHHH
VGLTRRDVIRPISEPIGKNGAIAILKGNIAPEGAVVKHSAVPKEMHKGILKARPFDSEEE
HCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCCCHHH
AISAIISKKINPGDAVFIRYEGPKGSGMPEMFYTTEAISSDKELSASIALITDGRFSGAS
HHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHEEHHHHCCCCCCCEEEEEEECCCCCCCC
KGPAIGHVSPEAAVGGPIALVEENDLIEIDIEKRILQLVGVNGQKLSEEAIDKVLVERKA
CCCCCCCCCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KWEKRENKYKSGILKIFSERAVSPMKGGYME
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA