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The map label for this gene is glcB [H]

Identifier: 226947514

GI number: 226947514

Start: 334695

End: 335168

Strand: Direct

Name: glcB [H]

Synonym: CLM_0348

Alternate gene names: 226947514

Gene position: 334695-335168 (Clockwise)

Preceding gene: 226947513

Following gene: 226947515

Centisome position: 8.05

GC content: 31.01

Gene sequence:

>474_bases
ATGTTTCAGATATTTAAGAAAAAGAAATTATATTCTCCATTAAGTGGGAAAAGTGTAGCATTAAGTAATGTACAGGATGA
AGTCTTTTCTTCTCTAATGATGGGAGATGGGATAGCTATTGATCCAATGGATCAGGTTATAGTAGCACCTTGCGATTGCT
TAGTTAAATTGATAATGAAAGGTTCAAAACATGCACTTGGTTTATTAATGAATAATGGTGTCGAAATATTAATTCATGTA
GGCATTGACACTGTAAGTTTAGAAGGCGAAGGTTTTGAAGTATTAGTTGAAGAAGGACAAAAGGTTAAGTTAGGAACACC
TTTACTAAAGTTTGATAAGGGTTATATTATATCAAAGGGATATTCTCCTATGACAATGATGATTATTACTGAAGCTAATG
GTAGCAATATAAATAAAAAATATGAGGATATTACTGTTGAAGGAGGAAAAACTCCAGTAATTGAATTTTCTTAA

Upstream 100 bases:

>100_bases
TAAGACAGTACCAAGTGCCAGTGTAGCAAAAGCTATTTTAGATGATTTAATTGAGGCTAATAAAAATTATTGGCCAGAAT
TAAAGTAAAGGAGATTAGAT

Downstream 100 bases:

>100_bases
TAAATAAAAATTTTATCGTAAATACATCTATAAAATATATTTAGTAAAAGCTCGGAAAGTGGATATACCATGTTCCGAGC
TATGCTTTAAACAAGATGCA

Product: PTS system glucose family transporter subunit IIA

Products: NA

Alternate protein names: EIICBA-Glc 2; Glucoside permease IIC component; PTS system glucoside-specific EIIC component; Glucoside-specific phosphotransferase enzyme IIB component; PTS system glucoside-specific EIIB component; Glucoside-specific phosphotransferase enzyme IIA component; PTS system glucoside-specific EIIA component [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MFQIFKKKKLYSPLSGKSVALSNVQDEVFSSLMMGDGIAIDPMDQVIVAPCDCLVKLIMKGSKHALGLLMNNGVEILIHV
GIDTVSLEGEGFEVLVEEGQKVKLGTPLLKFDKGYIISKGYSPMTMMIITEANGSNINKKYEDITVEGGKTPVIEFS

Sequences:

>Translated_157_residues
MFQIFKKKKLYSPLSGKSVALSNVQDEVFSSLMMGDGIAIDPMDQVIVAPCDCLVKLIMKGSKHALGLLMNNGVEILIHV
GIDTVSLEGEGFEVLVEEGQKVKLGTPLLKFDKGYIISKGYSPMTMMIITEANGSNINKKYEDITVEGGKTPVIEFS
>Mature_157_residues
MFQIFKKKKLYSPLSGKSVALSNVQDEVFSSLMMGDGIAIDPMDQVIVAPCDCLVKLIMKGSKHALGLLMNNGVEILIHV
GIDTVSLEGEGFEVLVEEGQKVKLGTPLLKFDKGYIISKGYSPMTMMIITEANGSNINKKYEDITVEGGKTPVIEFS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=151, Percent_Identity=39.7350993377483, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1786894, Length=150, Percent_Identity=38.6666666666667, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI1790159, Length=122, Percent_Identity=38.5245901639344, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011299 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17089; Mature: 17089

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQIFKKKKLYSPLSGKSVALSNVQDEVFSSLMMGDGIAIDPMDQVIVAPCDCLVKLIMK
CCCCCCCHHHCCCCCCCEEEECHHHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHHHC
GSKHALGLLMNNGVEILIHVGIDTVSLEGEGFEVLVEEGQKVKLGTPLLKFDKGYIISKG
CCCCEEEEEECCCEEEEEEECCEEEEECCCCEEEEEECCCEEEECCCEEEECCCEEEECC
YSPMTMMIITEANGSNINKKYEDITVEGGKTPVIEFS
CCCEEEEEEEECCCCCCCCEEEEEEEECCCCEEEEEC
>Mature Secondary Structure
MFQIFKKKKLYSPLSGKSVALSNVQDEVFSSLMMGDGIAIDPMDQVIVAPCDCLVKLIMK
CCCCCCCHHHCCCCCCCEEEECHHHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHHHC
GSKHALGLLMNNGVEILIHVGIDTVSLEGEGFEVLVEEGQKVKLGTPLLKFDKGYIISKG
CCCCEEEEEECCCEEEEEEECCEEEEECCCCEEEEEECCCEEEECCCEEEECCCEEEECC
YSPMTMMIITEANGSNINKKYEDITVEGGKTPVIEFS
CCCEEEEEEEECCCCCCCCEEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8602153; 10974121 [H]