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The map label for this gene is dnaN [H]

Identifier: 226947224

GI number: 226947224

Start: 1757

End: 2860

Strand: Direct

Name: dnaN [H]

Synonym: CLM_0002

Alternate gene names: 226947224

Gene position: 1757-2860 (Clockwise)

Preceding gene: 226947223

Following gene: 226947225

Centisome position: 0.04

GC content: 25.36

Gene sequence:

>1104_bases
ATGAAAATAACTTGTCAAAAAAACATTTTACTAGAAGGTATATCTATAGTTCAAAAAGCAGTAACTGGAAAATCAACTTT
ACCTATATTATCTGGAATACTTATTAGAGCTAATAAAAATGAATTGGTCTTAACTGGTTCTGATATGGATCTTAGCATTG
AGACTAAAGTAAAAGCTAACATTTTAGAAGAGGGAACTATAGTTTTAGATGCTAGACTTTTTGGTGAAATAATAAGGAAA
TTACCTAATGATCTAATAGAAATAAATACATTAGAAGATAATTCTATAGAAATAATATGTCAAAATTCTAGATTTAATCT
AATATATATGAATCCAGATGAATTTCCTAATCCACCTATTATCAATGAAAATATGATATTTTCCATAGGTGAAAGTAAAT
TAAAAAATATGATTAAAGGAACTATATTTGCTACAGCTCAAGATGAAACAAGGCCTATTCTTACAGGTGTTTTATTTCAA
ATAAAAGATAAAATGTTAAATTTAGTAGCTTTAGATGGTTACAGGTTAGCTTTAAGAAGTGAAGTTGTTGATAATGATAA
TACGATAAATGCTGTTATTCCAGGGAAAACATTAAATGAAGTTTCAAAAATTTTAGAAGAAGATAATGAAAATGTTAATA
TTACATTTACTCCTAATCATATTTTATTTAGCATAGGAGAAACAAAAATAATTTCAAGATTATTAGAAGGAGAATTTATT
AGCTATAAATCTATAATACCTGAAGAATTTAACTTAAAAATAATAGCTAAAAGATCAGAGCTTTTAAATTCTATAGAAAG
AGCCTCTCTTATGGCTAAAGAAGGAAACACTAATTTAGTTAAATTTGATTTTTCAGATGATAAAATTGTAATAACATCAA
ATTCTCAATTAGGAATGGTCCGAGAAGAATTAAAGGTTGTATTGCAAGGAGACGATTTACAAATTGCATTTAATTCAAAA
TATCTTTTAGATGTGTTAAAAACTATGGAAGATAATGAGGTTGTATTAGAATTTTCAAGTAGTGTAAGTCCTTGTATTAT
AAAAAATACAGAAATAAACAATTGCACTTACCTAGTTTTACCAGTAAGATTAAACAATAATTAA

Upstream 100 bases:

>100_bases
TATTAACATATCAACAAGCCCTACTACTACTACTACTATTTTATTATATTATCTATTTCTATTCTAACAATAGGAATTGT
TAATAAAGGAGGCTAATTTT

Downstream 100 bases:

>100_bases
TTAAAAGAGGTATTAAAAATGGTTGAAATAAAAATTAATTCAGAAATAATAAAATTAGATTCTTTCTTAAAATGGTCAGG
GGCAACAACTTTAGGTTCAG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKANILEEGTIVLDARLFGEIIRK
LPNDLIEINTLEDNSIEIICQNSRFNLIYMNPDEFPNPPIINENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQ
IKDKMLNLVALDGYRLALRSEVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI
SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMVREELKVVLQGDDLQIAFNSK
YLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVLPVRLNNN

Sequences:

>Translated_367_residues
MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKANILEEGTIVLDARLFGEIIRK
LPNDLIEINTLEDNSIEIICQNSRFNLIYMNPDEFPNPPIINENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQ
IKDKMLNLVALDGYRLALRSEVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI
SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMVREELKVVLQGDDLQIAFNSK
YLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVLPVRLNNN
>Mature_367_residues
MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKANILEEGTIVLDARLFGEIIRK
LPNDLIEINTLEDNSIEIICQNSRFNLIYMNPDEFPNPPIINENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQ
IKDKMLNLVALDGYRLALRSEVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI
SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMVREELKVVLQGDDLQIAFNSK
YLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVLPVRLNNN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=28.6103542234332, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41137; Mature: 41137

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKAN
CEEEECCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEEECCCCCEEEEEEEEEE
ILEEGTIVLDARLFGEIIRKLPNDLIEINTLEDNSIEIICQNSRFNLIYMNPDEFPNPPI
EECCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCE
INENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQIKDKMLNLVALDGYRLALRS
ECCCEEEEECHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEEH
EVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI
HHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCHHHHHHHHHCCCCE
SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMV
EHHHCCCCCCCEEEEEEHHHHHHHHHHHHHEEECCCCEEEEEECCCCEEEEECCCCCCHH
REELKVVLQGDDLQIAFNSKYLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVL
HHEEEEEEECCCEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCEEEEEE
PVRLNNN
EEEECCC
>Mature Secondary Structure
MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKAN
CEEEECCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEEECCCCCEEEEEEEEEE
ILEEGTIVLDARLFGEIIRKLPNDLIEINTLEDNSIEIICQNSRFNLIYMNPDEFPNPPI
EECCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCE
INENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQIKDKMLNLVALDGYRLALRS
ECCCEEEEECHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEEH
EVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI
HHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCHHHHHHHHHCCCCE
SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMV
EHHHCCCCCCCEEEEEEHHHHHHHHHHHHHEEECCCCEEEEEECCCCEEEEECCCCCCHH
REELKVVLQGDDLQIAFNSKYLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVL
HHEEEEEEECCCEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCEEEEEE
PVRLNNN
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA