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The map label for this gene is iolF [H]

Identifier: 226225399

GI number: 226225399

Start: 2906824

End: 2908092

Strand: Reverse

Name: iolF [H]

Synonym: Lm4b_02822

Alternate gene names: 226225399

Gene position: 2908092-2906824 (Counterclockwise)

Preceding gene: 226225401

Following gene: 226225398

Centisome position: 99.84

GC content: 39.8

Gene sequence:

>1269_bases
ATGGCTGGATCGAGTACAAGTAAAAAAGGAACGATAGCGGTATCTTTGACGAACTATTTAGATTCTGGATGTATTGTAGC
GGGAGCAAGTGGACTAACTTTGTGGGCAACACAATTCGGACTTAGCAGTTTTGCGGTGGGATTACTTGGGGCTGTGAGCG
CGAATGCGTTTGGTTCTGCAATTGGCGCACTTATTGGTGGTCATTTAGCAGATAAATATGGACGGAAATTTATTTATACG
TATAACATGTTGGTGTATATGCTTGGGGTTACAATCATTATGTTTGCAATGAATTTCCCAATGCTATTAATTGGATTTTT
AGTTACAGGTCTTTCTGTTGGGGCTGGGGTTCCGGCTTCATGGACATATATTTCTGAAATGGCGGATCCTAGTATAAGAG
CTAGAAATATTGGTATATCTCAATTTGCTTGGTCATGTGGGCCAGCAATTATCTTTACTTTAGGAATTATTGTATCTCCA
CTTGGACTTTTAGGTAACCGCTTACTTTTCTTATCTTTATTGATTGTAGCGTTTGTTGCTTGGCAGTTACAACGTAAATT
AGAGGAGTCGAAGGACTGGGAAGCGGAGCAAGTGAAAATGAAAGAAAGTGGTAATCGTTTGGAACACCCTTTTAAAACAG
CATTTTCTAGTATGGTAAATGTAAAATCAGTACTTTTCTTAGTGGGTGTGTACTTATTTTGGAATTTAGTAGCGGGTGCA
ATGGGATTCTTTATGCCGTTTGTATATGAAACGGTGGGTGGTCTTTCTAATACACAAGCGAATTTATTGCAAGCTGTATT
ATGGATACTCACAGCAGCTTCTACATACTTTGGCTTTGCTAAATTTGGTGATCGAGTAAGTCATCGTGGATTGTTTTTTG
TAGGGGCTTTAATGGCAGTTGCTTCTTGGGTTGTACTTACTTTTGTTGGAATGTCTTGGGTTGGCTTATGGACATTTGTT
ATTTTATGGGGTATTTCAGCAGGGATTGGTGCACAAGCTTTTTACGCACTTTGGTCAACAGAACTTTTTCCAACAAAATA
TCGTGGTGGGGTTCAAGGCGTGATGTTTTTCTTAGTTCGTGGTAGTACAGGTGTCTGGTCGATTGTGTTCCCAGTAATCT
TGGCAAATCTTGGCTTTACTGTGGCGGGGACAATTATGATTGGTTTACTTACAGTGTCGCTTTTAATCGGTGTAATTTGG
GCGCCGAAAACACGTGGCAGATCACTTGATGATATTACGAAGGAACGTTACGGAAATACAATTGATTAA

Upstream 100 bases:

>100_bases
TTGATACTTTAATTATAAACACAGGATATTGTAAAGCGCAATCATTAATTGTTTAATATAAAGTAAGCGCTTAACTAAAA
TAAGTAGGAGGGTTAAAAGA

Downstream 100 bases:

>100_bases
AGAAAAGGAGCTAGACCAGTGAAACATTATGTTGCAGTAGATATTGGTGCATCAAGTGGTCGATTAATTCTAGGGAAATT
GGTGAATGAAAAATTACAAT

Product: sugar transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAGSSTSKKGTIAVSLTNYLDSGCIVAGASGLTLWATQFGLSSFAVGLLGAVSANAFGSAIGALIGGHLADKYGRKFIYT
YNMLVYMLGVTIIMFAMNFPMLLIGFLVTGLSVGAGVPASWTYISEMADPSIRARNIGISQFAWSCGPAIIFTLGIIVSP
LGLLGNRLLFLSLLIVAFVAWQLQRKLEESKDWEAEQVKMKESGNRLEHPFKTAFSSMVNVKSVLFLVGVYLFWNLVAGA
MGFFMPFVYETVGGLSNTQANLLQAVLWILTAASTYFGFAKFGDRVSHRGLFFVGALMAVASWVVLTFVGMSWVGLWTFV
ILWGISAGIGAQAFYALWSTELFPTKYRGGVQGVMFFLVRGSTGVWSIVFPVILANLGFTVAGTIMIGLLTVSLLIGVIW
APKTRGRSLDDITKERYGNTID

Sequences:

>Translated_422_residues
MAGSSTSKKGTIAVSLTNYLDSGCIVAGASGLTLWATQFGLSSFAVGLLGAVSANAFGSAIGALIGGHLADKYGRKFIYT
YNMLVYMLGVTIIMFAMNFPMLLIGFLVTGLSVGAGVPASWTYISEMADPSIRARNIGISQFAWSCGPAIIFTLGIIVSP
LGLLGNRLLFLSLLIVAFVAWQLQRKLEESKDWEAEQVKMKESGNRLEHPFKTAFSSMVNVKSVLFLVGVYLFWNLVAGA
MGFFMPFVYETVGGLSNTQANLLQAVLWILTAASTYFGFAKFGDRVSHRGLFFVGALMAVASWVVLTFVGMSWVGLWTFV
ILWGISAGIGAQAFYALWSTELFPTKYRGGVQGVMFFLVRGSTGVWSIVFPVILANLGFTVAGTIMIGLLTVSLLIGVIW
APKTRGRSLDDITKERYGNTID
>Mature_421_residues
AGSSTSKKGTIAVSLTNYLDSGCIVAGASGLTLWATQFGLSSFAVGLLGAVSANAFGSAIGALIGGHLADKYGRKFIYTY
NMLVYMLGVTIIMFAMNFPMLLIGFLVTGLSVGAGVPASWTYISEMADPSIRARNIGISQFAWSCGPAIIFTLGIIVSPL
GLLGNRLLFLSLLIVAFVAWQLQRKLEESKDWEAEQVKMKESGNRLEHPFKTAFSSMVNVKSVLFLVGVYLFWNLVAGAM
GFFMPFVYETVGGLSNTQANLLQAVLWILTAASTYFGFAKFGDRVSHRGLFFVGALMAVASWVVLTFVGMSWVGLWTFVI
LWGISAGIGAQAFYALWSTELFPTKYRGGVQGVMFFLVRGSTGVWSIVFPVILANLGFTVAGTIMIGLLTVSLLIGVIWA
PKTRGRSLDDITKERYGNTID

Specific function: Minor myo-inositol uptake transporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786229, Length=416, Percent_Identity=24.2788461538462, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45523; Mature: 45392

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure 
AGSSTSKKGTIAVSLTNYLDSGCIVAGASGLTLWATQFGLSSFAVGLLGAVSANAFGSA
CCCCCCCCCEEEEEEHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGALIGGHLADKYGRKFIYTYNMLVYMLGVTIIMFAMNFPMLLIGFLVTGLSVGAGVPAS
HHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCH
WTYISEMADPSIRARNIGISQFAWSCGPAIIFTLGIIVSPLGLLGNRLLFLSLLIVAFVA
HHHHHHHCCCCHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQLQRKLEESKDWEAEQVKMKESGNRLEHPFKTAFSSMVNVKSVLFLVGVYLFWNLVAGA
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGFFMPFVYETVGGLSNTQANLLQAVLWILTAASTYFGFAKFGDRVSHRGLFFVGALMAV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
ASWVVLTFVGMSWVGLWTFVILWGISAGIGAQAFYALWSTELFPTKYRGGVQGVMFFLVR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHC
GSTGVWSIVFPVILANLGFTVAGTIMIGLLTVSLLIGVIWAPKTRGRSLDDITKERYGNT
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
ID
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7952181; 9384377; 1761221; 11807058 [H]