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The map label for this gene is ycnB [H]

Identifier: 226225134

GI number: 226225134

Start: 2636757

End: 2638238

Strand: Reverse

Name: ycnB [H]

Synonym: Lm4b_02555

Alternate gene names: 226225134

Gene position: 2638238-2636757 (Counterclockwise)

Preceding gene: 226225135

Following gene: 226225132

Centisome position: 90.58

GC content: 40.89

Gene sequence:

>1482_bases
TTGAATAGTACAGCAGTAGAACGGCCCGTTGATGTTAACGGGAAATCTTATAGTAGAAGTTTATTAGTAGTTACAATGAT
TATTGGGGCGTTTGTGGCGATCTTGAATCAGACGTTACTAGCGACGGCACTGCCGATGATTATGGATGATTTGCATATTA
CAGCAGCAACAGGGCAATGGTTGACGACGGCTTTCTTGCTAACAAATGGGATTATGATTCCGATTACGGCACTTTTAATT
GAAAAAATCAGTTCAAAAACATTATTTATTACAGCGATGACTGTATTTACAATTGGTACCATTATTGCGTCAGTGGCGGG
TTCTTTCCCGGTACTACTTACTGGTCGTATCGTTCAAGCAGCTGGTGCGGGGATTATGATGCCGTTGCTTCAAACAATCT
TCTTGCTGATTTTCCCTCGTGAAAAACGTGGGGCGGCGATGGGGCTGATGGGACTTGTTATTGCGTTTGCTCCAGCAATT
GGGCCAACTTTGTCTGGTTGGATTGTGGATTCGTATGATTGGCGCGTATTATTCCTTATTTTAATTCCAATCGCGGTTAT
CGATATAATTCTAGCGTTCTTTGGAATGAAAAAAGTAGTAAAATTAACTGATACAAAAATCGATTTTCTTTCCATTGTAA
TGTCTTCAATTGGTTTTGGTTCACTCCTTTATGGATTTAGTTCAGCAGGTAATGATGGTTGGGGAGATGCAACGGTTATC
ACGATGTTGATTGTTGGTGTCGTTGTTATTGCATTATTTGTTTGGCGCCAACTAGTTATTGATAACCCAATGCTTGAACT
ACATGTGTTTAAATATCCAGTATTTTCATTGTCTGTTATTCTTGGTTCGATTGTAACGATGGCAATGATTGGTGCGGAAA
TTGTATTGCCACTTTATATTCAAACAATTCGCGGGGAGTCGGCGCTTCAATCAGGTCTGTTATTACTTCCGGGTGCGATT
ATTATGGGGATAATGAGTCCGATTACGGGTATTATTTTCGATAAAATTGGGGCGAAATGGTTGACGATTACCGGGGTTAC
CATTTTGACTATCGGTACAATTCCATTTATGTTCTTAACGATGGATACGCCGCTTTGGTATATTGTTGTATTTTATGCTG
TGAGGTTCTTCGGGATTTCAATGGCGATGATGCCAGTTTCGACAGCGGGTATGAATGCACTTCCTAACCATTTGATTAAC
CACGGTTCGGCGGTAAATAATACGATTCGACAAATTGCTGGTTCGATTGGGACGGCGGTACTTATTACAGTGCTAACGAA
TGTAACGAAAGATAATATGCCAGGTAAAGCGCTTATGGCAACGGATCCGGCTAGTTTTGCTCAAAAAGCGCAAGATGCTA
GCTTGGACGGAATGCGTGCGGCGTTTATGGTTGCAGCGATTTTTGCGGCTATTGGGATGATCTTAAGTTTATTCCTGAAA
ACCAAAAAACAAGAGCCAATCGTCAAAGAATATACGAAATAA

Upstream 100 bases:

>100_bases
TAATTAGACTGACCAGTCACTTTTTGTTGACTTGTTGGTCTTTTTTTATTATACTTCTATTATTCATCTGGAAATTTCCA
CATGGGAAGGGGTATTAATT

Downstream 100 bases:

>100_bases
AAAAATGAAACAGGGTCAAACTTCCAATTTGGATGTTTGACCCTGTTTTTTTATACTTTTTTAAGGGTGACTTTCCAGCT
GGTGCCGGTGCCGACTGAAT

Product: drug-export protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MNSTAVERPVDVNGKSYSRSLLVVTMIIGAFVAILNQTLLATALPMIMDDLHITAATGQWLTTAFLLTNGIMIPITALLI
EKISSKTLFITAMTVFTIGTIIASVAGSFPVLLTGRIVQAAGAGIMMPLLQTIFLLIFPREKRGAAMGLMGLVIAFAPAI
GPTLSGWIVDSYDWRVLFLILIPIAVIDIILAFFGMKKVVKLTDTKIDFLSIVMSSIGFGSLLYGFSSAGNDGWGDATVI
TMLIVGVVVIALFVWRQLVIDNPMLELHVFKYPVFSLSVILGSIVTMAMIGAEIVLPLYIQTIRGESALQSGLLLLPGAI
IMGIMSPITGIIFDKIGAKWLTITGVTILTIGTIPFMFLTMDTPLWYIVVFYAVRFFGISMAMMPVSTAGMNALPNHLIN
HGSAVNNTIRQIAGSIGTAVLITVLTNVTKDNMPGKALMATDPASFAQKAQDASLDGMRAAFMVAAIFAAIGMILSLFLK
TKKQEPIVKEYTK

Sequences:

>Translated_493_residues
MNSTAVERPVDVNGKSYSRSLLVVTMIIGAFVAILNQTLLATALPMIMDDLHITAATGQWLTTAFLLTNGIMIPITALLI
EKISSKTLFITAMTVFTIGTIIASVAGSFPVLLTGRIVQAAGAGIMMPLLQTIFLLIFPREKRGAAMGLMGLVIAFAPAI
GPTLSGWIVDSYDWRVLFLILIPIAVIDIILAFFGMKKVVKLTDTKIDFLSIVMSSIGFGSLLYGFSSAGNDGWGDATVI
TMLIVGVVVIALFVWRQLVIDNPMLELHVFKYPVFSLSVILGSIVTMAMIGAEIVLPLYIQTIRGESALQSGLLLLPGAI
IMGIMSPITGIIFDKIGAKWLTITGVTILTIGTIPFMFLTMDTPLWYIVVFYAVRFFGISMAMMPVSTAGMNALPNHLIN
HGSAVNNTIRQIAGSIGTAVLITVLTNVTKDNMPGKALMATDPASFAQKAQDASLDGMRAAFMVAAIFAAIGMILSLFLK
TKKQEPIVKEYTK
>Mature_493_residues
MNSTAVERPVDVNGKSYSRSLLVVTMIIGAFVAILNQTLLATALPMIMDDLHITAATGQWLTTAFLLTNGIMIPITALLI
EKISSKTLFITAMTVFTIGTIIASVAGSFPVLLTGRIVQAAGAGIMMPLLQTIFLLIFPREKRGAAMGLMGLVIAFAPAI
GPTLSGWIVDSYDWRVLFLILIPIAVIDIILAFFGMKKVVKLTDTKIDFLSIVMSSIGFGSLLYGFSSAGNDGWGDATVI
TMLIVGVVVIALFVWRQLVIDNPMLELHVFKYPVFSLSVILGSIVTMAMIGAEIVLPLYIQTIRGESALQSGLLLLPGAI
IMGIMSPITGIIFDKIGAKWLTITGVTILTIGTIPFMFLTMDTPLWYIVVFYAVRFFGISMAMMPVSTAGMNALPNHLIN
HGSAVNNTIRQIAGSIGTAVLITVLTNVTKDNMPGKALMATDPASFAQKAQDASLDGMRAAFMVAAIFAAIGMILSLFLK
TKKQEPIVKEYTK

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=460, Percent_Identity=26.9565217391304, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788710, Length=440, Percent_Identity=26.1363636363636, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=26.555023923445, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1790195, Length=408, Percent_Identity=25, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1788392, Length=397, Percent_Identity=25.4408060453401, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319770, Length=335, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=327, Percent_Identity=23.2415902140673, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=228, Percent_Identity=25.8771929824561, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=365, Percent_Identity=23.2876712328767, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52897; Mature: 52897

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTAVERPVDVNGKSYSRSLLVVTMIIGAFVAILNQTLLATALPMIMDDLHITAATGQW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHH
LTTAFLLTNGIMIPITALLIEKISSKTLFITAMTVFTIGTIIASVAGSFPVLLTGRIVQA
HHHHHHHHCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGAGIMMPLLQTIFLLIFPREKRGAAMGLMGLVIAFAPAIGPTLSGWIVDSYDWRVLFLI
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHH
LIPIAVIDIILAFFGMKKVVKLTDTKIDFLSIVMSSIGFGSLLYGFSSAGNDGWGDATVI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHH
TMLIVGVVVIALFVWRQLVIDNPMLELHVFKYPVFSLSVILGSIVTMAMIGAEIVLPLYI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTIRGESALQSGLLLLPGAIIMGIMSPITGIIFDKIGAKWLTITGVTILTIGTIPFMFLT
HHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHH
MDTPLWYIVVFYAVRFFGISMAMMPVSTAGMNALPNHLINHGSAVNNTIRQIAGSIGTAV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LITVLTNVTKDNMPGKALMATDPASFAQKAQDASLDGMRAAFMVAAIFAAIGMILSLFLK
HHHHHHHHHCCCCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKKQEPIVKEYTK
HCCCCCHHHHCCC
>Mature Secondary Structure
MNSTAVERPVDVNGKSYSRSLLVVTMIIGAFVAILNQTLLATALPMIMDDLHITAATGQW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHH
LTTAFLLTNGIMIPITALLIEKISSKTLFITAMTVFTIGTIIASVAGSFPVLLTGRIVQA
HHHHHHHHCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGAGIMMPLLQTIFLLIFPREKRGAAMGLMGLVIAFAPAIGPTLSGWIVDSYDWRVLFLI
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHH
LIPIAVIDIILAFFGMKKVVKLTDTKIDFLSIVMSSIGFGSLLYGFSSAGNDGWGDATVI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHH
TMLIVGVVVIALFVWRQLVIDNPMLELHVFKYPVFSLSVILGSIVTMAMIGAEIVLPLYI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTIRGESALQSGLLLLPGAIIMGIMSPITGIIFDKIGAKWLTITGVTILTIGTIPFMFLT
HHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHH
MDTPLWYIVVFYAVRFFGISMAMMPVSTAGMNALPNHLINHGSAVNNTIRQIAGSIGTAV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LITVLTNVTKDNMPGKALMATDPASFAQKAQDASLDGMRAAFMVAAIFAAIGMILSLFLK
HHHHHHHHHCCCCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKKQEPIVKEYTK
HCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]