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The map label for this gene is pncA [C]

Identifier: 226225118

GI number: 226225118

Start: 2620911

End: 2621543

Strand: Reverse

Name: pncA [C]

Synonym: Lm4b_02539

Alternate gene names: 226225118

Gene position: 2621543-2620911 (Counterclockwise)

Preceding gene: 226225119

Following gene: 226225117

Centisome position: 90.0

GC content: 40.44

Gene sequence:

>633_bases
ATGAAAATTGCTGCTTTTGATATTGATGCACAAAAAGGTTTTACGCCGCTTTGTCCCGATGAATTACCGGTTTCGGGCGG
AGACAAAATTGTGCCAGAACTTAATTTTATGGCAAGTCTTGCTTCTCTTCGAATTGGTAGTAAGGACGCCCATCCGCAAA
ATGCCGTATGGGTAGTGGATTCCAAAGACGAAATGTTGGCTGAACTCGAATATCCAAATGCAGACCGTACTTGGGTAAGG
CACTGTGAACCAGGTACAAAAGGTTTTGAATTATTAGATGGTTTACCAGTTGTGACTGATTATGATTACTTTATTTGGAA
AGGTATGGAGCCGGATATTCATCCGTACGGCGCTTGTTACCACGATATTGTTGAACAGCTTTCAACAGGTGTTCTAGAAT
ATTTACGGGAACAAAAAGTCGATTTAGTTCTTGTTGGAGGTTTAGCTTTTGATTTTTGTGTGAAAACTACGGTTATACAG
TTAAGTAGGGCAGGCTTTTCTGTATTAGTTTATTTACCAGCGACACGTGCACTATCTGAACAAGGTTTTGATGAAACGAA
AGAGCAATTAGCAAATACCGAGAAAGTGCAACTTATCGAAACAAGGGAGGAACTTGTCGCCTATTGCCAGTAA

Upstream 100 bases:

>100_bases
ACTCTTTGAAGAAGATAGAGAGCACGCGCTAGAGCTAGAAGAAGTAACTCGTTTCGGCCAGTTTGCCCAACTAACGTATA
AAAACTTGGAGGAAAATTAA

Downstream 100 bases:

>100_bases
TTATATTGGAAGAGGTGAGGGGAATGAGGAAAGTTGGTATATTTCCTAGTATGATAATTCTTTTCACAGTAGGAATTTCT
GCTGTGTTATATCCGCTACT

Product: nicotinamidase

Products: nicotinate; NH3

Alternate protein names: Pyrazinamidase/Nicotinamidase; Isochorismatase Hydrolase; Isochorismatase Family Protein; Bifunctional Pyrazinamidase/Nicotinamidase; Amidase; Pyrazinamidase; Nicotinamidase/Pyrazinamidase; Pyrazinamidase/Nicotinamidase PncA; Nicotinamidase-Like Amidase

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKIAAFDIDAQKGFTPLCPDELPVSGGDKIVPELNFMASLASLRIGSKDAHPQNAVWVVDSKDEMLAELEYPNADRTWVR
HCEPGTKGFELLDGLPVVTDYDYFIWKGMEPDIHPYGACYHDIVEQLSTGVLEYLREQKVDLVLVGGLAFDFCVKTTVIQ
LSRAGFSVLVYLPATRALSEQGFDETKEQLANTEKVQLIETREELVAYCQ

Sequences:

>Translated_210_residues
MKIAAFDIDAQKGFTPLCPDELPVSGGDKIVPELNFMASLASLRIGSKDAHPQNAVWVVDSKDEMLAELEYPNADRTWVR
HCEPGTKGFELLDGLPVVTDYDYFIWKGMEPDIHPYGACYHDIVEQLSTGVLEYLREQKVDLVLVGGLAFDFCVKTTVIQ
LSRAGFSVLVYLPATRALSEQGFDETKEQLANTEKVQLIETREELVAYCQ
>Mature_210_residues
MKIAAFDIDAQKGFTPLCPDELPVSGGDKIVPELNFMASLASLRIGSKDAHPQNAVWVVDSKDEMLAELEYPNADRTWVR
HCEPGTKGFELLDGLPVVTDYDYFIWKGMEPDIHPYGACYHDIVEQLSTGVLEYLREQKVDLVLVGGLAFDFCVKTTVIQ
LSRAGFSVLVYLPATRALSEQGFDETKEQLANTEKVQLIETREELVAYCQ

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.19

Molecular weight: Translated: 23479; Mature: 23479

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAAFDIDAQKGFTPLCPDELPVSGGDKIVPELNFMASLASLRIGSKDAHPQNAVWVVD
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
SKDEMLAELEYPNADRTWVRHCEPGTKGFELLDGLPVVTDYDYFIWKGMEPDIHPYGACY
CCHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHCCCCCEECCCEEEEECCCCCCCCCHHHH
HDIVEQLSTGVLEYLREQKVDLVLVGGLAFDFCVKTTVIQLSRAGFSVLVYLPATRALSE
HHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
QGFDETKEQLANTEKVQLIETREELVAYCQ
CCCHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIAAFDIDAQKGFTPLCPDELPVSGGDKIVPELNFMASLASLRIGSKDAHPQNAVWVVD
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
SKDEMLAELEYPNADRTWVRHCEPGTKGFELLDGLPVVTDYDYFIWKGMEPDIHPYGACY
CCHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHCCCCCEECCCEEEEECCCCCCCCCHHHH
HDIVEQLSTGVLEYLREQKVDLVLVGGLAFDFCVKTTVIQLSRAGFSVLVYLPATRALSE
HHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHH
QGFDETKEQLANTEKVQLIETREELVAYCQ
CCCHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide; H2O

Specific reaction: nicotinamide + H2O = nicotinate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA