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The map label for this gene is argS

Identifier: 226225108

GI number: 226225108

Start: 2612145

End: 2613815

Strand: Reverse

Name: argS

Synonym: Lm4b_02529

Alternate gene names: 226225108

Gene position: 2613815-2612145 (Counterclockwise)

Preceding gene: 226225109

Following gene: 226225107

Centisome position: 89.74

GC content: 38.0

Gene sequence:

>1671_bases
ATGAATGTCATGCAAGAGAACCAAATCAAATTAATTGAACATATCAAACAAGGAGTTGTTCAAGCGGTTGGTTTAGAAGA
AGCGGAAGTACCAGAAATTTTACTAGAAGTACCAAAAGATAAAAAGCATGGAGATTATTCGACTAATATAGCGATGCAAC
TTGCTAGAGTAGCTAAGAAAGCCCCTCGCCAAATTGCCGAAAGTATCGTTCCAGAACTAAAAAAAGACAACAAATTAATT
AAAGAAGTAGAAATCGCTGGGCCGGGTTTTATCAACTTCTACCTAGATAATGCTTATTTAACTGATTTAGTTCCTGTGAT
TTTAACCGAAGATAAAAAATACGGAGAGTCTGATTTTGGTAAAGGAGAAAAATTCCAAATTGAATTTGTTTCCGCGAACC
CAACTGGTGACTTGCATTTAGGACATGCTCGTGGCGCAGCAATTGGCGACTCGTTAGCAAATATTATGAAAATGGCCGGA
TTTGATGTTTCAAGAGAATACTACATTAATGATGCTGGAAATCAAATTAATAATTTAGTTCTTTCAGCAGAAGCGCGTTA
CTTTGAAGCACTAGGTTTAGATTCTGAATTCCCAGAGGATGGCTACCGTGGTGCGGATATTATTTCGCTTGGGAAAGATT
TAGCTGCTAAATACGGCGATAAATATGTGCACACAAGCGAAGAAGAGCGCCGTTCCGTTTTCCGTGTAGATGCATTAGCG
TTTGAAACAGGAAAATTACGCGCTGACTTAGAAGAATTCCGCGTTTCCTTTGATGAGTGGTTCTCAGAAACTTCTTTATA
CGAAGAAAATAAAGTATTACCAGCGTTAGAACGTTTACGCGAAAATGGCTATATTTATGAGCAAGATGGCGCGACATGGT
TAAGAACGACTGATTTTGAAGATGACAAAGACCGTGTTTTAATCAAATCAGATGGTAGTTATACGTATTTCTTACCAGAT
ATCGCGTACCACTTAAACAAATTAGAGCGTGGCTTTGATGTGTTAATTGATATTTGGGGAGCTGATCACCACGGCTATAT
TCCTCGTATGCGTGCTGCAATTGAAGCGCTTGGCTACTCACCAAATCAATTAGAAGTAGAAATCATTCAGCTAGTTCACT
TATTTGAAGACGGTGTTCAAGTTAAAATGAGTAAACGTACTGGTAAATCAGTTACAATGCGTGACTTAATTGAAGAAGTT
GGACTGGATGCAACAAGATATTTCTTTGCAATGCGTAGTTCAGATACACATATGAACTTTGATATGAGCTTAGCGAAATC
TACATCTAATGATAATCCAGTTTATTATGTACAATATGCGCACGCCAGAATTTCTAGCATTTTACGTTCTGGTAAAGAAC
AAGGTCTAGAGGTAACAAAAGACGCAGACATGAGCTTACTGCAAACAGAAGCAGAATATGATTTATTAAAAGTGTTAGGT
GAGTTCGCGGATGTTGTTGCGGAAGCTGCTACAAAAAGAGCGCCACACCGTATCGTTCGCTACTTAAATGATTTAGCGTC
TTCCTTCCACCGTTTCTACAATAGCAATAAAGTACTTGATATGGATAATTTAGAAGTAACAAAAGCAAGACTAGCACTTA
TTAAGACTGCGCAAATTACCCTTAGAAATGGTTTGACGCTTTTAGGTGTATCAGCACCAGAAAAAATGTGA

Upstream 100 bases:

>100_bases
CTGATGTACTGAGTGAAGTCGCATTTCAATATGATTTGCTAAGCCACGGCGAATACATTGGCTCATATACTGTTTTAATG
AAAATAGAGGAGGACGCAGA

Downstream 100 bases:

>100_bases
TATCCTAAATTTATAGGTTACTCGTTGACGTCATAACCAAAATGTACTAAAGTTAATAGAAACATAGGTAATTGAAAAAA
CGGGGAGAGCATATGATGAA

Product: arginyl-tRNA synthetase

Products: NA

Alternate protein names: Arginine--tRNA ligase; ArgRS [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MNVMQENQIKLIEHIKQGVVQAVGLEEAEVPEILLEVPKDKKHGDYSTNIAMQLARVAKKAPRQIAESIVPELKKDNKLI
KEVEIAGPGFINFYLDNAYLTDLVPVILTEDKKYGESDFGKGEKFQIEFVSANPTGDLHLGHARGAAIGDSLANIMKMAG
FDVSREYYINDAGNQINNLVLSAEARYFEALGLDSEFPEDGYRGADIISLGKDLAAKYGDKYVHTSEEERRSVFRVDALA
FETGKLRADLEEFRVSFDEWFSETSLYEENKVLPALERLRENGYIYEQDGATWLRTTDFEDDKDRVLIKSDGSYTYFLPD
IAYHLNKLERGFDVLIDIWGADHHGYIPRMRAAIEALGYSPNQLEVEIIQLVHLFEDGVQVKMSKRTGKSVTMRDLIEEV
GLDATRYFFAMRSSDTHMNFDMSLAKSTSNDNPVYYVQYAHARISSILRSGKEQGLEVTKDADMSLLQTEAEYDLLKVLG
EFADVVAEAATKRAPHRIVRYLNDLASSFHRFYNSNKVLDMDNLEVTKARLALIKTAQITLRNGLTLLGVSAPEKM

Sequences:

>Translated_556_residues
MNVMQENQIKLIEHIKQGVVQAVGLEEAEVPEILLEVPKDKKHGDYSTNIAMQLARVAKKAPRQIAESIVPELKKDNKLI
KEVEIAGPGFINFYLDNAYLTDLVPVILTEDKKYGESDFGKGEKFQIEFVSANPTGDLHLGHARGAAIGDSLANIMKMAG
FDVSREYYINDAGNQINNLVLSAEARYFEALGLDSEFPEDGYRGADIISLGKDLAAKYGDKYVHTSEEERRSVFRVDALA
FETGKLRADLEEFRVSFDEWFSETSLYEENKVLPALERLRENGYIYEQDGATWLRTTDFEDDKDRVLIKSDGSYTYFLPD
IAYHLNKLERGFDVLIDIWGADHHGYIPRMRAAIEALGYSPNQLEVEIIQLVHLFEDGVQVKMSKRTGKSVTMRDLIEEV
GLDATRYFFAMRSSDTHMNFDMSLAKSTSNDNPVYYVQYAHARISSILRSGKEQGLEVTKDADMSLLQTEAEYDLLKVLG
EFADVVAEAATKRAPHRIVRYLNDLASSFHRFYNSNKVLDMDNLEVTKARLALIKTAQITLRNGLTLLGVSAPEKM
>Mature_556_residues
MNVMQENQIKLIEHIKQGVVQAVGLEEAEVPEILLEVPKDKKHGDYSTNIAMQLARVAKKAPRQIAESIVPELKKDNKLI
KEVEIAGPGFINFYLDNAYLTDLVPVILTEDKKYGESDFGKGEKFQIEFVSANPTGDLHLGHARGAAIGDSLANIMKMAG
FDVSREYYINDAGNQINNLVLSAEARYFEALGLDSEFPEDGYRGADIISLGKDLAAKYGDKYVHTSEEERRSVFRVDALA
FETGKLRADLEEFRVSFDEWFSETSLYEENKVLPALERLRENGYIYEQDGATWLRTTDFEDDKDRVLIKSDGSYTYFLPD
IAYHLNKLERGFDVLIDIWGADHHGYIPRMRAAIEALGYSPNQLEVEIIQLVHLFEDGVQVKMSKRTGKSVTMRDLIEEV
GLDATRYFFAMRSSDTHMNFDMSLAKSTSNDNPVYYVQYAHARISSILRSGKEQGLEVTKDADMSLLQTEAEYDLLKVLG
EFADVVAEAATKRAPHRIVRYLNDLASSFHRFYNSNKVLDMDNLEVTKARLALIKTAQITLRNGLTLLGVSAPEKM

Specific function: Unknown

COG id: COG0018

COG function: function code J; Arginyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15149476, Length=622, Percent_Identity=25.2411575562701, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1788184, Length=595, Percent_Identity=27.3949579831933, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71985061, Length=592, Percent_Identity=23.8175675675676, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71985068, Length=553, Percent_Identity=23.6889692585895, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320548, Length=569, Percent_Identity=25.6590509666081, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI18859963, Length=621, Percent_Identity=24.1545893719807, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571570, Length=493, Percent_Identity=23.9350912778905, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 789 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR001278
- InterPro:   IPR015945
- InterPro:   IPR005148
- InterPro:   IPR008909
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF03485 Arg_tRNA_synt_N; PF05746 DALR_1; PF00750 tRNA-synt_1d [H]

EC number: =6.1.1.19 [H]

Molecular weight: Translated: 62811; Mature: 62811

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVMQENQIKLIEHIKQGVVQAVGLEEAEVPEILLEVPKDKKHGDYSTNIAMQLARVAKK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
APRQIAESIVPELKKDNKLIKEVEIAGPGFINFYLDNAYLTDLVPVILTEDKKYGESDFG
HHHHHHHHHHHHHHHCCHHHHHHEECCCCEEEEEECCHHHHHHHHHEEECCCCCCCCCCC
KGEKFQIEFVSANPTGDLHLGHARGAAIGDSLANIMKMAGFDVSREYYINDAGNQINNLV
CCCEEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHEE
LSAEARYFEALGLDSEFPEDGYRGADIISLGKDLAAKYGDKYVHTSEEERRSVFRVDALA
EECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
FETGKLRADLEEFRVSFDEWFSETSLYEENKVLPALERLRENGYIYEQDGATWLRTTDFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCEEEEECCCC
DDKDRVLIKSDGSYTYFLPDIAYHLNKLERGFDVLIDIWGADHHGYIPRMRAAIEALGYS
CCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCC
PNQLEVEIIQLVHLFEDGVQVKMSKRTGKSVTMRDLIEEVGLDATRYFFAMRSSDTHMNF
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHEEEEECCCCCEEE
DMSLAKSTSNDNPVYYVQYAHARISSILRSGKEQGLEVTKDADMSLLQTEAEYDLLKVLG
EEHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHH
EFADVVAEAATKRAPHRIVRYLNDLASSFHRFYNSNKVLDMDNLEVTKARLALIKTAQIT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHEEH
LRNGLTLLGVSAPEKM
HHCCCEEEECCCCCCC
>Mature Secondary Structure
MNVMQENQIKLIEHIKQGVVQAVGLEEAEVPEILLEVPKDKKHGDYSTNIAMQLARVAKK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
APRQIAESIVPELKKDNKLIKEVEIAGPGFINFYLDNAYLTDLVPVILTEDKKYGESDFG
HHHHHHHHHHHHHHHCCHHHHHHEECCCCEEEEEECCHHHHHHHHHEEECCCCCCCCCCC
KGEKFQIEFVSANPTGDLHLGHARGAAIGDSLANIMKMAGFDVSREYYINDAGNQINNLV
CCCEEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHEE
LSAEARYFEALGLDSEFPEDGYRGADIISLGKDLAAKYGDKYVHTSEEERRSVFRVDALA
EECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
FETGKLRADLEEFRVSFDEWFSETSLYEENKVLPALERLRENGYIYEQDGATWLRTTDFE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCEEEEECCCC
DDKDRVLIKSDGSYTYFLPDIAYHLNKLERGFDVLIDIWGADHHGYIPRMRAAIEALGYS
CCCCEEEEEECCCEEEEHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCC
PNQLEVEIIQLVHLFEDGVQVKMSKRTGKSVTMRDLIEEVGLDATRYFFAMRSSDTHMNF
CCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHEEEEECCCCCEEE
DMSLAKSTSNDNPVYYVQYAHARISSILRSGKEQGLEVTKDADMSLLQTEAEYDLLKVLG
EEHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHH
EFADVVAEAATKRAPHRIVRYLNDLASSFHRFYNSNKVLDMDNLEVTKARLALIKTAQIT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHEEH
LRNGLTLLGVSAPEKM
HHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]