Click here to switch to the map view.

The map label for this gene is eno

Identifier: 226225003

GI number: 226225003

Start: 2498953

End: 2500245

Strand: Reverse

Name: eno

Synonym: Lm4b_02424

Alternate gene names: 226225003

Gene position: 2500245-2498953 (Counterclockwise)

Preceding gene: 226225004

Following gene: 226225002

Centisome position: 85.84

GC content: 40.68

Gene sequence:

>1293_bases
ATGTCTATTATTACTGAAGTTTATGCTCGCGAAGTCTTAGATTCCCGTGGTAACCCAACTGTTGAGGTTGAAGTTTATAC
TGAAGCTGGTGCGTTTGGTCGCGCTTTAGTTCCAAGTGGTGCTTCAACTGGTGAATACGAAGCTGTAGAATTACGCGATG
GCGACAAAGCTCGTTACCTTGGAAAAGGTGTTTTAAAAGCTGTTGAAAACGTAAACGACATTATTGCTGACAAAATTATC
GGTTTTGACGTAACTGATCAAATCGGAATTGACAAAGCAATGATCGAACTTGATGGTACACCTAACAAAGGTAAATTAGG
TGCTAACGCTATTCTTGGTGTTTCTTTAGCTGCTGCTCGTGCTGCTGCTGACGAACTAGGCGTACATTTATATGAATATC
TTGGCGGAGTGAACGGTAAAGTTCTTCCAGTTCCAATGATGAACATCCTTAACGGCGGAGAACATGCTGATAACAATGTC
GACGTTCAAGAATTTATGGTAATGCCTGTTGGAGCTCCAAACTTTAAAGAAGCTCTACGTATGGGTGCTGAAATCCTACA
CGCACTTAAAGCTGTACTTAAAGGTAAAGGCTTAAACACTGGTGTTGGTGATGAAGGTGGATTCGCTCCAAACCTTAAAT
CCAATGAAGAAGCTCTTGAAACAATCATGCAAGCAATTAAAGATGCTGGTTACAAACCTGGCGAAGAAGTTAAACTTGCG
ATGGATGCTGCATCAAGTGAGTTCTATAACCGCGAAACTGGTAAATACGAACTTAAAGGTGAAGGCGTAACTCGTACTTC
TGAAGAAATGGTAACTTGGTATGAAGAAATGATTACTAAATACCCAATCATCTCTATTGAAGATGGCCTAGACGAAAACG
ACTGGGACGGATTCAAACTACTTACAGAACGTATTGGTGACCGCGTTCAATTAGTAGGTGACGATCTATTTGTAACTAAC
ACAACTAAACTTAAAGAAGGTATCGAAAAAGGTATCGCTAACTCCATCCTAATCAAAGTTAACCAAATCGGTACTTTGAC
TGAAACATTGGATGCAATTGAAATGGCTAAACGCGCTGGCTACACTGCAGTTATCTCTCACCGTTCTGGTGAAACAGAAG
ATTCCACAATCGCTGACATTGCAGTAGCTACAAACGCTGGTCAAATCAAAACTGGTGCGCCTACTCGTACTGACCGTGTT
GCAAAATATAACCAATTACTCCGCATCGAAGACAATTTGGCTGATCTTGCTGAATACCATGGTAACGAAACTTTCTACAA
CTTAAAAAAATAA

Upstream 100 bases:

>100_bases
CTACAATAAATACGAATTTAAAAACGGATGGGTCTTTGAATATGAAGTGAATTTTTTGTTCATTTCATTCAATTAATCCC
AACAAAGGAGAGAATTATAA

Downstream 100 bases:

>100_bases
GTTGATTGAATAAAACGCTAAGCTCCGCGAAAAGTAACATTTTCGCGGAGTTTTTTCTGTTTTAAAAATAGCGAGAGGGG
TAATGATTTGGAAGAGGAGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSIITEVYAREVLDSRGNPTVEVEVYTEAGAFGRALVPSGASTGEYEAVELRDGDKARYLGKGVLKAVENVNDIIADKII
GFDVTDQIGIDKAMIELDGTPNKGKLGANAILGVSLAAARAAADELGVHLYEYLGGVNGKVLPVPMMNILNGGEHADNNV
DVQEFMVMPVGAPNFKEALRMGAEILHALKAVLKGKGLNTGVGDEGGFAPNLKSNEEALETIMQAIKDAGYKPGEEVKLA
MDAASSEFYNRETGKYELKGEGVTRTSEEMVTWYEEMITKYPIISIEDGLDENDWDGFKLLTERIGDRVQLVGDDLFVTN
TTKLKEGIEKGIANSILIKVNQIGTLTETLDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPTRTDRV
AKYNQLLRIEDNLADLAEYHGNETFYNLKK

Sequences:

>Translated_430_residues
MSIITEVYAREVLDSRGNPTVEVEVYTEAGAFGRALVPSGASTGEYEAVELRDGDKARYLGKGVLKAVENVNDIIADKII
GFDVTDQIGIDKAMIELDGTPNKGKLGANAILGVSLAAARAAADELGVHLYEYLGGVNGKVLPVPMMNILNGGEHADNNV
DVQEFMVMPVGAPNFKEALRMGAEILHALKAVLKGKGLNTGVGDEGGFAPNLKSNEEALETIMQAIKDAGYKPGEEVKLA
MDAASSEFYNRETGKYELKGEGVTRTSEEMVTWYEEMITKYPIISIEDGLDENDWDGFKLLTERIGDRVQLVGDDLFVTN
TTKLKEGIEKGIANSILIKVNQIGTLTETLDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPTRTDRV
AKYNQLLRIEDNLADLAEYHGNETFYNLKK
>Mature_429_residues
SIITEVYAREVLDSRGNPTVEVEVYTEAGAFGRALVPSGASTGEYEAVELRDGDKARYLGKGVLKAVENVNDIIADKIIG
FDVTDQIGIDKAMIELDGTPNKGKLGANAILGVSLAAARAAADELGVHLYEYLGGVNGKVLPVPMMNILNGGEHADNNVD
VQEFMVMPVGAPNFKEALRMGAEILHALKAVLKGKGLNTGVGDEGGFAPNLKSNEEALETIMQAIKDAGYKPGEEVKLAM
DAASSEFYNRETGKYELKGEGVTRTSEEMVTWYEEMITKYPIISIEDGLDENDWDGFKLLTERIGDRVQLVGDDLFVTNT
TKLKEGIEKGIANSILIKVNQIGTLTETLDAIEMAKRAGYTAVISHRSGETEDSTIADIAVATNAGQIKTGAPTRTDRVA
KYNQLLRIEDNLADLAEYHGNETFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=438, Percent_Identity=52.2831050228311, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=438, Percent_Identity=52.2831050228311, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=51.508120649652, Blast_Score=431, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=51.5011547344111, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=436, Percent_Identity=47.2477064220183, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=338, Percent_Identity=24.5562130177515, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI169201757, Length=338, Percent_Identity=24.5562130177515, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI239744207, Length=338, Percent_Identity=24.5562130177515, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=65.6542056074766, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=52.3148148148148, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=52.3148148148148, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=44.0414507772021, Blast_Score=171, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=50.7972665148064, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=50.4608294930876, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=50.2304147465438, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=50.2304147465438, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321968, Length=439, Percent_Identity=50.1138952164009, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=51.0440835266821, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=51.0440835266821, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=51.0440835266821, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=51.0440835266821, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=51.0440835266821, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=51.0440835266821, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46487; Mature: 46356

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIITEVYAREVLDSRGNPTVEVEVYTEAGAFGRALVPSGASTGEYEAVELRDGDKARYL
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCHHHHH
GKGVLKAVENVNDIIADKIIGFDVTDQIGIDKAMIELDGTPNKGKLGANAILGVSLAAAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADELGVHLYEYLGGVNGKVLPVPMMNILNGGEHADNNVDVQEFMVMPVGAPNFKEALR
HHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHHHHH
MGAEILHALKAVLKGKGLNTGVGDEGGFAPNLKSNEEALETIMQAIKDAGYKPGEEVKLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDAASSEFYNRETGKYELKGEGVTRTSEEMVTWYEEMITKYPIISIEDGLDENDWDGFKL
EHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTERIGDRVQLVGDDLFVTNTTKLKEGIEKGIANSILIKVNQIGTLTETLDAIEMAKRAG
HHHHHCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVATNAGQIKTGAPTRTDRVAKYNQLLRIEDNLADLAEYH
CEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCCHHHHHHHHHHEEEHHHHHHHHHHC
GNETFYNLKK
CCCCEEECCC
>Mature Secondary Structure 
SIITEVYAREVLDSRGNPTVEVEVYTEAGAFGRALVPSGASTGEYEAVELRDGDKARYL
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCEEEEEECCCCHHHHH
GKGVLKAVENVNDIIADKIIGFDVTDQIGIDKAMIELDGTPNKGKLGANAILGVSLAAAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADELGVHLYEYLGGVNGKVLPVPMMNILNGGEHADNNVDVQEFMVMPVGAPNFKEALR
HHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHHHHH
MGAEILHALKAVLKGKGLNTGVGDEGGFAPNLKSNEEALETIMQAIKDAGYKPGEEVKLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDAASSEFYNRETGKYELKGEGVTRTSEEMVTWYEEMITKYPIISIEDGLDENDWDGFKL
EHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHH
LTERIGDRVQLVGDDLFVTNTTKLKEGIEKGIANSILIKVNQIGTLTETLDAIEMAKRAG
HHHHHCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVATNAGQIKTGAPTRTDRVAKYNQLLRIEDNLADLAEYH
CEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCCHHHHHHHHHHEEEHHHHHHHHHHC
GNETFYNLKK
CCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]