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The map label for this gene is est [H]

Identifier: 226225000

GI number: 226225000

Start: 2496899

End: 2497642

Strand: Reverse

Name: est [H]

Synonym: Lm4b_02421

Alternate gene names: 226225000

Gene position: 2497642-2496899 (Counterclockwise)

Preceding gene: 226225001

Following gene: 226224999

Centisome position: 85.75

GC content: 40.05

Gene sequence:

>744_bases
ATGAGTGCGGATCGTAGCTTTACGTTAAAAGCGGGAAATCGTGCTGTTTTGCTCTTGCATGGCTTTGCAGGGACTACAGA
AGACGTAAGAGAACTTGGAGAAATTCTAGCAGAAAATGGATACACAGTGCATGCACCAAACTTCAGAGGTCACGGGGATG
AACCCGCTATTTTCTTGAAAACAACACCGGAAATGTGGTATGAAGATGCGGTGGCTGGATATCGTCAACTTGAAAAAGAC
GGATATAATGAAATTGCAATTGTCGGTGTCGCAATGGGCGGAGTTTTTGCGCTTAAAATGGCAGAATCGTTTTCACCAAA
AGCGATTGTTCCACTATGCGCCAACGTCAACCGCAAAATGCGCTATATCCCAATCGAAAACTATTTAACTAAACAATTAA
AGAAACAAGGTATAGTAGAACAAGAAGCAGATCAAATGCTAAAAAACTACCTTCCTGAAATAGACGTAATGACGGAAGCA
CGTGCTACTTTCTACAAAAACGTAGCGCGAGACATTGAAAAAATTCATGTACCAACCATGATTGGCCAAGGCTGTCAAGA
TGAGGAAATTGATGCAGATAACGCCAATTATATCTTTAAGCACATTCATACAAATGATAAACAATTGTGTTTCTATGCAG
GGTCAGGGCATGACATCGTGAACGACTGTGAGAAAGACATTTTAGAAGAAGACTTAATTTACTTCTTAGACGATTTAGTT
TGGCTAGAGGAAAAAGTTGTTTAA

Upstream 100 bases:

>100_bases
TTGGTCTATTGGTCAGAACTCAAAAGGTAGCTTATTTAGTTCAGATGGGCTACAATAGAGTGGATTATAAAAGTGATGAT
ATTAGGAGGATTGCGTAAAA

Downstream 100 bases:

>100_bases
CTATTGTATAGAAACAAACCTTTACGTATAATACCAAATATGGTAAAATTAATCTTAGTCAAATTGGGCGGTTTTAGGAG
GAAAGAACAATGAGTACAGT

Product: carboxylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSADRSFTLKAGNRAVLLLHGFAGTTEDVRELGEILAENGYTVHAPNFRGHGDEPAIFLKTTPEMWYEDAVAGYRQLEKD
GYNEIAIVGVAMGGVFALKMAESFSPKAIVPLCANVNRKMRYIPIENYLTKQLKKQGIVEQEADQMLKNYLPEIDVMTEA
RATFYKNVARDIEKIHVPTMIGQGCQDEEIDADNANYIFKHIHTNDKQLCFYAGSGHDIVNDCEKDILEEDLIYFLDDLV
WLEEKVV

Sequences:

>Translated_247_residues
MSADRSFTLKAGNRAVLLLHGFAGTTEDVRELGEILAENGYTVHAPNFRGHGDEPAIFLKTTPEMWYEDAVAGYRQLEKD
GYNEIAIVGVAMGGVFALKMAESFSPKAIVPLCANVNRKMRYIPIENYLTKQLKKQGIVEQEADQMLKNYLPEIDVMTEA
RATFYKNVARDIEKIHVPTMIGQGCQDEEIDADNANYIFKHIHTNDKQLCFYAGSGHDIVNDCEKDILEEDLIYFLDDLV
WLEEKVV
>Mature_246_residues
SADRSFTLKAGNRAVLLLHGFAGTTEDVRELGEILAENGYTVHAPNFRGHGDEPAIFLKTTPEMWYEDAVAGYRQLEKDG
YNEIAIVGVAMGGVFALKMAESFSPKAIVPLCANVNRKMRYIPIENYLTKQLKKQGIVEQEADQMLKNYLPEIDVMTEAR
ATFYKNVARDIEKIHVPTMIGQGCQDEEIDADNANYIFKHIHTNDKQLCFYAGSGHDIVNDCEKDILEEDLIYFLDDLVW
LEEKVV

Specific function: Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones [H]

COG id: COG1647

COG function: function code R; Esterase/lipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019149
- InterPro:   IPR012354 [H]

Pfam domain/function: PF09752 DUF2048 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 27929; Mature: 27798

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADRSFTLKAGNRAVLLLHGFAGTTEDVRELGEILAENGYTVHAPNFRGHGDEPAIFLK
CCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEE
TTPEMWYEDAVAGYRQLEKDGYNEIAIVGVAMGGVFALKMAESFSPKAIVPLCANVNRKM
ECHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCCEEHHHHCCCCCCE
RYIPIENYLTKQLKKQGIVEQEADQMLKNYLPEIDVMTEARATFYKNVARDIEKIHVPTM
EEEEHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
IGQGCQDEEIDADNANYIFKHIHTNDKQLCFYAGSGHDIVNDCEKDILEEDLIYFLDDLV
HCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
WLEEKVV
HHHHHCC
>Mature Secondary Structure 
SADRSFTLKAGNRAVLLLHGFAGTTEDVRELGEILAENGYTVHAPNFRGHGDEPAIFLK
CCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEE
TTPEMWYEDAVAGYRQLEKDGYNEIAIVGVAMGGVFALKMAESFSPKAIVPLCANVNRKM
ECHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCCEEHHHHCCCCCCE
RYIPIENYLTKQLKKQGIVEQEADQMLKNYLPEIDVMTEARATFYKNVARDIEKIHVPTM
EEEEHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
IGQGCQDEEIDADNANYIFKHIHTNDKQLCFYAGSGHDIVNDCEKDILEEDLIYFLDDLV
HCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
WLEEKVV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]