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The map label for this gene is smpB

Identifier: 226224996

GI number: 226224996

Start: 2492466

End: 2492930

Strand: Reverse

Name: smpB

Synonym: Lm4b_02417

Alternate gene names: 226224996

Gene position: 2492930-2492466 (Counterclockwise)

Preceding gene: 226224997

Following gene: 226224994

Centisome position: 85.59

GC content: 38.92

Gene sequence:

>465_bases
ATGCCAAAAGGTGATGGTAAGCTAGTTGCGCAAAATAAAAAAGCGCGCCACGATTACGCAATTGAAGAAACTTTTGAGGC
TGGCATTGTCCTGCAAGGTACTGAAATTAAATCCGTAAGAAACGCACGGGTAAACTTAAAAGATTCCTATGCACGTATCG
ACAAAGGGGAAATTTTCTTACACAATATGCATATTAGTCCTTATGAACAAGGGAACCGCTACAATCATGATCCACTAAGA
ACGCGCAAATTGCTTTTACATAAGAAGCAAATCAGCCGTTTAATTGGAGAAACGAAAGAGTCCGGTTATTCGATTGTTCC
GCTAAAAATGTATATTAAAGATGGCTACGCAAAAGTACTCATCGGTGTAGCTCGAGGTAAAAAGAAATACGATAAACGCC
AAGACTTAAAACAAAAAGAAGCAAAACGTGATATCGAACGCGCTTTTAAAGAGCGCCAACAATAA

Upstream 100 bases:

>100_bases
TACAAAACCTAAAAAGAAAAAGAAGAAAAAACCTTTTTATCAAGGTGTAGCAAAAGAAAGCCCGAAGAAGAAAAAACGTC
GCTAGAAAGGATGGGGAGTT

Downstream 100 bases:

>100_bases
AAGCCTAAATCTAGTCATTCAACCAAAAGTTGGAGAAGCTAGATTTAGGCTTTTTTTATATGTCGATTTTGGTGAATTCG
TTTAAAGAGAGATTTTGAAG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MPKGDGKLVAQNKKARHDYAIEETFEAGIVLQGTEIKSVRNARVNLKDSYARIDKGEIFLHNMHISPYEQGNRYNHDPLR
TRKLLLHKKQISRLIGETKESGYSIVPLKMYIKDGYAKVLIGVARGKKKYDKRQDLKQKEAKRDIERAFKERQQ

Sequences:

>Translated_154_residues
MPKGDGKLVAQNKKARHDYAIEETFEAGIVLQGTEIKSVRNARVNLKDSYARIDKGEIFLHNMHISPYEQGNRYNHDPLR
TRKLLLHKKQISRLIGETKESGYSIVPLKMYIKDGYAKVLIGVARGKKKYDKRQDLKQKEAKRDIERAFKERQQ
>Mature_153_residues
PKGDGKLVAQNKKARHDYAIEETFEAGIVLQGTEIKSVRNARVNLKDSYARIDKGEIFLHNMHISPYEQGNRYNHDPLRT
RKLLLHKKQISRLIGETKESGYSIVPLKMYIKDGYAKVLIGVARGKKKYDKRQDLKQKEAKRDIERAFKERQQ

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=143, Percent_Identity=46.1538461538462, Blast_Score=137, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_LISIN (P66861)

Other databases:

- EMBL:   AL596172
- PIR:   AI1749
- RefSeq:   NP_471872.1
- ProteinModelPortal:   P66861
- SMR:   P66861
- GeneID:   1131354
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin2542
- GenoList:   LIN2542
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   LINN272626:LIN2542-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17924; Mature: 17793

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKGDGKLVAQNKKARHDYAIEETFEAGIVLQGTEIKSVRNARVNLKDSYARIDKGEIFL
CCCCCCCEEECCCCCCCCHHHHHHHCCCEEEECCHHHHHHHCCCCCCHHHHHCCCCEEEE
HNMHISPYEQGNRYNHDPLRTRKLLLHKKQISRLIGETKESGYSIVPLKMYIKDGYAKVL
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHH
IGVARGKKKYDKRQDLKQKEAKRDIERAFKERQQ
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKGDGKLVAQNKKARHDYAIEETFEAGIVLQGTEIKSVRNARVNLKDSYARIDKGEIFL
CCCCCCEEECCCCCCCCHHHHHHHCCCEEEECCHHHHHHHCCCCCCHHHHHCCCCEEEE
HNMHISPYEQGNRYNHDPLRTRKLLLHKKQISRLIGETKESGYSIVPLKMYIKDGYAKVL
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHH
IGVARGKKKYDKRQDLKQKEAKRDIERAFKERQQ
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669