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The map label for this gene is vanS [H]

Identifier: 226224968

GI number: 226224968

Start: 2463005

End: 2464147

Strand: Reverse

Name: vanS [H]

Synonym: Lm4b_02389

Alternate gene names: 226224968

Gene position: 2464147-2463005 (Counterclockwise)

Preceding gene: 226224969

Following gene: 226224967

Centisome position: 84.6

GC content: 37.88

Gene sequence:

>1143_bases
TTGAAATCTAAACTGGAACTGTTGTTTACGAAAATTAGCCATGTGATGAATGTTTGGCAGGCGACTTTTTTTGCAGCTGT
TTCTTGGATTTTGGCGTTGATTAGCATCCAAACTATTTATTTATGGAGCATTTCCATTCGGGATGATTCTTTGTTTTTAC
GGGAAGTGTCACAAGCTATTGTTCAATTATTTGGAACGAATAAATATACGCGAATCATGCAATCTTTTTGGATGATGTCG
CTAAAATTTCTGATTATCTTTTTAATAGCTTGTCTATTCTTTGCTTTTTTCTATCGTTTGATGCGTCAAAGAATTTTGAA
AAAACAACTGCGTACGATTAATTTTGCGCTTAACGACGGGAAGCCAGTTGACACAGAAAGTTTTGTGCCGGAAATTCAAG
AACTGGAACGTAACATCGAAAATATGCGGGAACGGCAGAAAAAATTAATGCAGCAGGAAGAACAGGCCCAGCAAGCTAGA
AATGATTTGATTACGAATGTTTCGCATGATTTGCGAACACCACTTACATCCATTTTAGGCTATTTAAGTTATATACATGA
AGACCGTTATCGCGATGAAATTGAGCTGCGTTATTACACCGAGCTTGTGTACGGGAAAGCGCGTCATTTGCACAAACTTA
TTGATGATTTGTTTTCTTATACAAGGCTCGATAGCGTGGATTACCAATTAAAGAAAGATGAGTTAGACATTATTGAGTTG
CTACGGCAATTAGTGGCGGAGTACGACGGGCGAGCTGCAGAACGAAATATGCAGATTGTCGAACATTTTGATGCTAACAA
ACTAATTATTGGCGGTGATGGCAACCAAATCATGCGCTTATTTGAGAATTTATTTTCGAATGCCCTTAGATACGGAGAAG
GCAACAAACAAATTGATGTTAGTGCTAAGAAAGAAGATAATATGGCTGTTGTGCGCGTAACGAATTATGGTGAAGAAATT
CCTAATGTCGATTTACCTTACTTGTTCGAGCGCTTTTACAAAGTAGATAAAAATCGGACGACGACTGGAACCGGATTAGG
CCTTGCGATTGCTAAATCTATTGTTGAAAAACATGGCGGAATTGTTACTGCAGAAAGTAAAAAACGCCAAACCAGTTTTA
TCGTCAAATTGCCGCTAATTTAA

Upstream 100 bases:

>100_bases
AAGACAGTAATGGTCCATATTAGTAATTTGCGTGATAAGTTACGCGAGGCTATGGGTGGAGAAAATGTGATTAAGACTAT
TTGGGGAGTAGGGTACAAAA

Downstream 100 bases:

>100_bases
TCTTTAACAATTCTTAAAAGTTTCTTTCCGCGTGATTTATGTTCTTTCTGTAGAATATAAATCACGGAGGAGAGGAAAAT
GAAAAAACATATTGTTTTAA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKSKLELLFTKISHVMNVWQATFFAAVSWILALISIQTIYLWSISIRDDSLFLREVSQAIVQLFGTNKYTRIMQSFWMMS
LKFLIIFLIACLFFAFFYRLMRQRILKKQLRTINFALNDGKPVDTESFVPEIQELERNIENMRERQKKLMQQEEQAQQAR
NDLITNVSHDLRTPLTSILGYLSYIHEDRYRDEIELRYYTELVYGKARHLHKLIDDLFSYTRLDSVDYQLKKDELDIIEL
LRQLVAEYDGRAAERNMQIVEHFDANKLIIGGDGNQIMRLFENLFSNALRYGEGNKQIDVSAKKEDNMAVVRVTNYGEEI
PNVDLPYLFERFYKVDKNRTTTGTGLGLAIAKSIVEKHGGIVTAESKKRQTSFIVKLPLI

Sequences:

>Translated_380_residues
MKSKLELLFTKISHVMNVWQATFFAAVSWILALISIQTIYLWSISIRDDSLFLREVSQAIVQLFGTNKYTRIMQSFWMMS
LKFLIIFLIACLFFAFFYRLMRQRILKKQLRTINFALNDGKPVDTESFVPEIQELERNIENMRERQKKLMQQEEQAQQAR
NDLITNVSHDLRTPLTSILGYLSYIHEDRYRDEIELRYYTELVYGKARHLHKLIDDLFSYTRLDSVDYQLKKDELDIIEL
LRQLVAEYDGRAAERNMQIVEHFDANKLIIGGDGNQIMRLFENLFSNALRYGEGNKQIDVSAKKEDNMAVVRVTNYGEEI
PNVDLPYLFERFYKVDKNRTTTGTGLGLAIAKSIVEKHGGIVTAESKKRQTSFIVKLPLI
>Mature_380_residues
MKSKLELLFTKISHVMNVWQATFFAAVSWILALISIQTIYLWSISIRDDSLFLREVSQAIVQLFGTNKYTRIMQSFWMMS
LKFLIIFLIACLFFAFFYRLMRQRILKKQLRTINFALNDGKPVDTESFVPEIQELERNIENMRERQKKLMQQEEQAQQAR
NDLITNVSHDLRTPLTSILGYLSYIHEDRYRDEIELRYYTELVYGKARHLHKLIDDLFSYTRLDSVDYQLKKDELDIIEL
LRQLVAEYDGRAAERNMQIVEHFDANKLIIGGDGNQIMRLFENLFSNALRYGEGNKQIDVSAKKEDNMAVVRVTNYGEEI
PNVDLPYLFERFYKVDKNRTTTGTGLGLAIAKSIVEKHGGIVTAESKKRQTSFIVKLPLI

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=233, Percent_Identity=34.7639484978541, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786783, Length=304, Percent_Identity=26.3157894736842, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786600, Length=234, Percent_Identity=29.9145299145299, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786912, Length=246, Percent_Identity=28.0487804878049, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=25.6198347107438, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788713, Length=236, Percent_Identity=26.6949152542373, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789808, Length=286, Percent_Identity=26.2237762237762, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788279, Length=304, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081816, Length=257, Percent_Identity=26.8482490272374, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787894, Length=219, Percent_Identity=28.7671232876712, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082128, Length=302, Percent_Identity=23.1788079470199, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=25.7261410788382, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789149, Length=259, Percent_Identity=26.2548262548263, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790346, Length=221, Percent_Identity=27.6018099547511, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=25, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790861, Length=251, Percent_Identity=23.1075697211155, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789403, Length=291, Percent_Identity=22.680412371134, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44515; Mature: 44515

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKLELLFTKISHVMNVWQATFFAAVSWILALISIQTIYLWSISIRDDSLFLREVSQAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHH
VQLFGTNKYTRIMQSFWMMSLKFLIIFLIACLFFAFFYRLMRQRILKKQLRTINFALNDG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
KPVDTESFVPEIQELERNIENMRERQKKLMQQEEQAQQARNDLITNVSHDLRTPLTSILG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLSYIHEDRYRDEIELRYYTELVYGKARHLHKLIDDLFSYTRLDSVDYQLKKDELDIIEL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHH
LRQLVAEYDGRAAERNMQIVEHFDANKLIIGGDGNQIMRLFENLFSNALRYGEGNKQIDV
HHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
SAKKEDNMAVVRVTNYGEEIPNVDLPYLFERFYKVDKNRTTTGTGLGLAIAKSIVEKHGG
ECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
IVTAESKKRQTSFIVKLPLI
EEEECCCCCCCEEEEEECCC
>Mature Secondary Structure
MKSKLELLFTKISHVMNVWQATFFAAVSWILALISIQTIYLWSISIRDDSLFLREVSQAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHH
VQLFGTNKYTRIMQSFWMMSLKFLIIFLIACLFFAFFYRLMRQRILKKQLRTINFALNDG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
KPVDTESFVPEIQELERNIENMRERQKKLMQQEEQAQQARNDLITNVSHDLRTPLTSILG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLSYIHEDRYRDEIELRYYTELVYGKARHLHKLIDDLFSYTRLDSVDYQLKKDELDIIEL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHH
LRQLVAEYDGRAAERNMQIVEHFDANKLIIGGDGNQIMRLFENLFSNALRYGEGNKQIDV
HHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
SAKKEDNMAVVRVTNYGEEIPNVDLPYLFERFYKVDKNRTTTGTGLGLAIAKSIVEKHGG
ECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
IVTAESKKRQTSFIVKLPLI
EEEECCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]