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The map label for this gene is lacF [H]

Identifier: 226224908

GI number: 226224908

Start: 2396192

End: 2396506

Strand: Reverse

Name: lacF [H]

Synonym: Lm4b_02327b

Alternate gene names: 226224908

Gene position: 2396506-2396192 (Counterclockwise)

Preceding gene: 226224909

Following gene: 226224907

Centisome position: 82.28

GC content: 38.1

Gene sequence:

>315_bases
ATGGAAGGCACTGAATTACAGTCATTTAAAATAATATCAAGTGCAGGAGATGCCTCTTCCTCGTTTCTAAAAGCAATTCG
TCACGCAGAAAAAAGTGAATTTGCGGAAGCAGAAGCATGTATTGAACAAGCAAATCAATCTTTAAGAGAAGCGCATCATG
TGCAAACATCCTTGATTCAAGAGGAAGCGGGCGGGGACTCAAAAGAAGTATCCTTATTGCTTATTCATGCGCAAGATCAT
CTGATGAATGCTTTTGTTTACTGCGATTTAGTTAAGTCAGTACTTAACTTGTATAAGCGTTTGGATGAAAAGTAA

Upstream 100 bases:

>100_bases
AAGAAGATTATGGCAACATGAATGCAGTACCAATTCTAATCGCTGCATTTAAAAAGATTAAAGAAGCTGGAACGAATACA
TTTAAGACGGAGGGAAACTA

Downstream 100 bases:

>100_bases
GGAGTAGTGACAATGAAACTAATAATGAATGCAGATGATTTTGGGTTCACAAGAGCAATCAATTACGGAATCTTTGATGC
GCACAATTTAGGCGTTTTGA

Product: PTS system, cellobiose-specific enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 104; Mature: 104

Protein sequence:

>104_residues
MEGTELQSFKIISSAGDASSSFLKAIRHAEKSEFAEAEACIEQANQSLREAHHVQTSLIQEEAGGDSKEVSLLLIHAQDH
LMNAFVYCDLVKSVLNLYKRLDEK

Sequences:

>Translated_104_residues
MEGTELQSFKIISSAGDASSSFLKAIRHAEKSEFAEAEACIEQANQSLREAHHVQTSLIQEEAGGDSKEVSLLLIHAQDH
LMNAFVYCDLVKSVLNLYKRLDEK
>Mature_104_residues
MEGTELQSFKIISSAGDASSSFLKAIRHAEKSEFAEAEACIEQANQSLREAHHVQTSLIQEEAGGDSKEVSLLLIHAQDH
LMNAFVYCDLVKSVLNLYKRLDEK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=91, Percent_Identity=39.5604395604396, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 11550; Mature: 11550

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGTELQSFKIISSAGDASSSFLKAIRHAEKSEFAEAEACIEQANQSLREAHHVQTSLIQ
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAGGDSKEVSLLLIHAQDHLMNAFVYCDLVKSVLNLYKRLDEK
HHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEGTELQSFKIISSAGDASSSFLKAIRHAEKSEFAEAEACIEQANQSLREAHHVQTSLIQ
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAGGDSKEVSLLLIHAQDHLMNAFVYCDLVKSVLNLYKRLDEK
HHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA