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The map label for this gene is nagB [H]

Identifier: 226224903

GI number: 226224903

Start: 2390432

End: 2391163

Strand: Reverse

Name: nagB [H]

Synonym: Lm4b_02322

Alternate gene names: 226224903

Gene position: 2391163-2390432 (Counterclockwise)

Preceding gene: 226224904

Following gene: 226224902

Centisome position: 82.09

GC content: 38.25

Gene sequence:

>732_bases
ATGAAATTAATCAGGACAAAAACATACGAAGAAATGTCACAAGAAGCGCTCGAAGTTGTCAAACAAGTCATTAACGAAAA
TGATAATCCGGTAATTAATACAACTACCGGAGCAAGTTTTGATGGAATGTTCGAGGGACTTGTAAAAGGAATTAATGCAG
GCGAAATTCCAATCGAAAAAGTATTTTTAATGAATTTGGATGAGTATGTGGCTAAACGCGATGCTTCCTTCACTGTTTAC
ACATATATGCACCAAAAATTCTATGATTTAATTAACAAAATGCCAAAAAGAGTCGAATTATTGGACGGAAGCCTAGCTGA
CTTTACTGACGAAATTGCCCGCTATAAGAAAATTTTGGCTGAAAATGAGCGCGATTTGCAAATTCTAGGCTTAGGGGTAA
ATGGACATCTTGGGGCTAATGAACCCGGAACACCATTTGATGCGCGATTGTTTTTGGCAGATAGCGATGAATCTACTATC
AAAAGCACCATCATGTACAACAACTTAAAAGAAGACGAAGCGCCATCCCAAATGCTTACACTTGGATTAGCTGATATGAT
GGATGCAAAACAAATCTTAGTCACTGCTTCTGGTGAACGAAAAGCAGAAGCCGTTAAAGGTTTACTTGAGGGACCAATTG
ATGAAAGTTGTCCAGCGTCGATTTTACGTAACCATCCGAATGTCGTATTTATTATTGATGAAGCGGCAGGGTCATTACTT
ACTAATCACTAA

Upstream 100 bases:

>100_bases
AAATCCGTTGAAGAAGATGGGTTATACGCAAGTTTTCTAAAGCATGGTTTGCTTTAGAAAAAATAGTTCGGGTAATGAAA
GCTTATAGAGGAGGATTTTT

Downstream 100 bases:

>100_bases
AAAGGGGGCAAAACCATGAGAAAGTTATACACGTATTTCGTTTTAATTCTCGGTGTGGCGATGATTGGTCTTGGAATTTA
TTTAATGTTTAATCCAAGTA

Product: N-acetylglucosamine-6-phosphate isomerase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKLIRTKTYEEMSQEALEVVKQVINENDNPVINTTTGASFDGMFEGLVKGINAGEIPIEKVFLMNLDEYVAKRDASFTVY
TYMHQKFYDLINKMPKRVELLDGSLADFTDEIARYKKILAENERDLQILGLGVNGHLGANEPGTPFDARLFLADSDESTI
KSTIMYNNLKEDEAPSQMLTLGLADMMDAKQILVTASGERKAEAVKGLLEGPIDESCPASILRNHPNVVFIIDEAAGSLL
TNH

Sequences:

>Translated_243_residues
MKLIRTKTYEEMSQEALEVVKQVINENDNPVINTTTGASFDGMFEGLVKGINAGEIPIEKVFLMNLDEYVAKRDASFTVY
TYMHQKFYDLINKMPKRVELLDGSLADFTDEIARYKKILAENERDLQILGLGVNGHLGANEPGTPFDARLFLADSDESTI
KSTIMYNNLKEDEAPSQMLTLGLADMMDAKQILVTASGERKAEAVKGLLEGPIDESCPASILRNHPNVVFIIDEAAGSLL
TNH
>Mature_243_residues
MKLIRTKTYEEMSQEALEVVKQVINENDNPVINTTTGASFDGMFEGLVKGINAGEIPIEKVFLMNLDEYVAKRDASFTVY
TYMHQKFYDLINKMPKRVELLDGSLADFTDEIARYKKILAENERDLQILGLGVNGHLGANEPGTPFDARLFLADSDESTI
KSTIMYNNLKEDEAPSQMLTLGLADMMDAKQILVTASGERKAEAVKGLLEGPIDESCPASILRNHPNVVFIIDEAAGSLL
TNH

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI13027378, Length=243, Percent_Identity=27.5720164609054, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI19923881, Length=243, Percent_Identity=26.7489711934156, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786893, Length=244, Percent_Identity=26.2295081967213, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789530, Length=240, Percent_Identity=28.3333333333333, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI48994958, Length=235, Percent_Identity=27.2340425531915, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554876, Length=242, Percent_Identity=31.404958677686, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24581960, Length=243, Percent_Identity=27.5720164609054, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19920764, Length=243, Percent_Identity=27.5720164609054, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 26949; Mature: 26949

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIRTKTYEEMSQEALEVVKQVINENDNPVINTTTGASFDGMFEGLVKGINAGEIPIEK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCHHH
VFLMNLDEYVAKRDASFTVYTYMHQKFYDLINKMPKRVELLDGSLADFTDEIARYKKILA
HHHHCHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHH
ENERDLQILGLGVNGHLGANEPGTPFDARLFLADSDESTIKSTIMYNNLKEDEAPSQMLT
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHH
LGLADMMDAKQILVTASGERKAEAVKGLLEGPIDESCPASILRNHPNVVFIIDEAAGSLL
HHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEECCCCHHH
TNH
CCC
>Mature Secondary Structure
MKLIRTKTYEEMSQEALEVVKQVINENDNPVINTTTGASFDGMFEGLVKGINAGEIPIEK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCHHH
VFLMNLDEYVAKRDASFTVYTYMHQKFYDLINKMPKRVELLDGSLADFTDEIARYKKILA
HHHHCHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHH
ENERDLQILGLGVNGHLGANEPGTPFDARLFLADSDESTIKSTIMYNNLKEDEAPSQMLT
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHH
LGLADMMDAKQILVTASGERKAEAVKGLLEGPIDESCPASILRNHPNVVFIIDEAAGSLL
HHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEECCCCHHH
TNH
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA