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The map label for this gene is tcyK [H]

Identifier: 226224894

GI number: 226224894

Start: 2380874

End: 2381683

Strand: Reverse

Name: tcyK [H]

Synonym: Lm4b_02313

Alternate gene names: 226224894

Gene position: 2381683-2380874 (Counterclockwise)

Preceding gene: 226224895

Following gene: 226224893

Centisome position: 81.77

GC content: 36.17

Gene sequence:

>810_bases
ATGAAAAAGAAATATGGGATTTTGGCACTGGCTCTTACAGCCGCATTAACATTAAGCGCATGTGGAGGAAAAGAAGAACC
AGAAGCAGCGAATCAAAAAGTACAAACAATCACGGTTGGCACTGGAACACAATTTCCAAATGTCTGCTTTTTGGATGAAA
ATGGGAAATTAACCGGTTATGATGTCGAACTAGTAAAAGAAATCGATAAACGTTTGCCCGGCTATAAATTTAAATTTAAA
ACGATGGATTTTTCTAATTTACTCGTTAGCCTTGGAGCTGGAAAAGTAGACATTGTCGCGCATCAAATGGAAAAAAGTAA
AGAACGCGAGAAGAAATTTTTATTTAATGATGTAGCATACAATAATTTCCCATTACAATTGACGGTATTAGACAGTAATA
ACAGCATAAACAGCACAAAAGATTTAGCTGGAAAACGCGTTATCACAAGCGCAACATCGAATGGTGCGCTCGTATTGAAA
AAAATTAATGAAGAACAAGGCAATAATTTTGAAATTGCTTATGAAGGTCAAGGGTCAAATGATACAGCGAACCAACTGAA
AACTGGCCGCGCTGATGCAACGATTTCTACACCATTCGCGGTAGATTTCCAAAATAAAACAAGCGCTATCAAAGAAAAAG
TAGTTGGCGATGTTCTATCTAATGCGAAAGTATACTTCATGCTCGGAAAAGATGAAACAAAACTAAGCAAAGATGTAGAT
GAGGCGCTTCAATCTATTATTGATGATGGAACACTAAAAAAATTAAGCGAAAAATGGCTTGGCGCAGATTATTCAAAAGA
ACAATATTAA

Upstream 100 bases:

>100_bases
GGTGATGTGGGTGTTATGATGCGGAAAATCTTAATTCCGGAAAAATTCGACGCCACACTACTCGGAATACCTAGCTGGAC
ATAAAAAAGGGGGAAAAAGG

Downstream 100 bases:

>100_bases
ACAAGGGAGGGTGCCATTAAAATGGATAAAGCGTTCGATGTAAAAATGATTGCTGATTTTATTCCAACGTTAGCTGGTTA
TTTACCGATTACGTTGTATA

Product: amino acid ABC transporter (binding protein)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKKKYGILALALTAALTLSACGGKEEPEAANQKVQTITVGTGTQFPNVCFLDENGKLTGYDVELVKEIDKRLPGYKFKFK
TMDFSNLLVSLGAGKVDIVAHQMEKSKEREKKFLFNDVAYNNFPLQLTVLDSNNSINSTKDLAGKRVITSATSNGALVLK
KINEEQGNNFEIAYEGQGSNDTANQLKTGRADATISTPFAVDFQNKTSAIKEKVVGDVLSNAKVYFMLGKDETKLSKDVD
EALQSIIDDGTLKKLSEKWLGADYSKEQY

Sequences:

>Translated_269_residues
MKKKYGILALALTAALTLSACGGKEEPEAANQKVQTITVGTGTQFPNVCFLDENGKLTGYDVELVKEIDKRLPGYKFKFK
TMDFSNLLVSLGAGKVDIVAHQMEKSKEREKKFLFNDVAYNNFPLQLTVLDSNNSINSTKDLAGKRVITSATSNGALVLK
KINEEQGNNFEIAYEGQGSNDTANQLKTGRADATISTPFAVDFQNKTSAIKEKVVGDVLSNAKVYFMLGKDETKLSKDVD
EALQSIIDDGTLKKLSEKWLGADYSKEQY
>Mature_269_residues
MKKKYGILALALTAALTLSACGGKEEPEAANQKVQTITVGTGTQFPNVCFLDENGKLTGYDVELVKEIDKRLPGYKFKFK
TMDFSNLLVSLGAGKVDIVAHQMEKSKEREKKFLFNDVAYNNFPLQLTVLDSNNSINSTKDLAGKRVITSATSNGALVLK
KINEEQGNNFEIAYEGQGSNDTANQLKTGRADATISTPFAVDFQNKTSAIKEKVVGDVLSNAKVYFMLGKDETKLSKDVD
EALQSIIDDGTLKKLSEKWLGADYSKEQY

Specific function: Part of the ABC transporter complex tcyJKLMN involved in L-cystine import. Is also involved in cystathionine, djenkolate, and S-methylcysteine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=267, Percent_Identity=31.4606741573034, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787031, Length=269, Percent_Identity=29.368029739777, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788649, Length=278, Percent_Identity=26.978417266187, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787088, Length=266, Percent_Identity=26.6917293233083, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787085, Length=264, Percent_Identity=24.6212121212121, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29519; Mature: 29519

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKYGILALALTAALTLSACGGKEEPEAANQKVQTITVGTGTQFPNVCFLDENGKLTGY
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEEECCCCCCCEEEECCCCCEECC
DVELVKEIDKRLPGYKFKFKTMDFSNLLVSLGAGKVDIVAHQMEKSKEREKKFLFNDVAY
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCC
NNFPLQLTVLDSNNSINSTKDLAGKRVITSATSNGALVLKKINEEQGNNFEIAYEGQGSN
CCCCEEEEEEECCCCCCCHHHHCCCEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCC
DTANQLKTGRADATISTPFAVDFQNKTSAIKEKVVGDVLSNAKVYFMLGKDETKLSKDVD
CHHHHHHCCCCCCEECCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
EALQSIIDDGTLKKLSEKWLGADYSKEQY
HHHHHHHCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKKYGILALALTAALTLSACGGKEEPEAANQKVQTITVGTGTQFPNVCFLDENGKLTGY
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEEECCCCCCCEEEECCCCCEECC
DVELVKEIDKRLPGYKFKFKTMDFSNLLVSLGAGKVDIVAHQMEKSKEREKKFLFNDVAY
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCC
NNFPLQLTVLDSNNSINSTKDLAGKRVITSATSNGALVLKKINEEQGNNFEIAYEGQGSN
CCCCEEEEEEECCCCCCCHHHHCCCEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCC
DTANQLKTGRADATISTPFAVDFQNKTSAIKEKVVGDVLSNAKVYFMLGKDETKLSKDVD
CHHHHHHCCCCCCEECCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
EALQSIIDDGTLKKLSEKWLGADYSKEQY
HHHHHHHCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]