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The map label for this gene is pbuO [H]

Identifier: 226224862

GI number: 226224862

Start: 2349802

End: 2351094

Strand: Direct

Name: pbuO [H]

Synonym: Lm4b_02281

Alternate gene names: 226224862

Gene position: 2349802-2351094 (Clockwise)

Preceding gene: 226224860

Following gene: 226224863

Centisome position: 80.67

GC content: 39.13

Gene sequence:

>1293_bases
ATGTTTCAATTAAAAGCAAACGGTACAGATGTTAAAACGGAAGTTATCTCTGGTTTTACTACATTCCTAACGATGGTTTA
TATCGTAGTAGTTAACCCAGCCATTCTTTCCGCAGCAGGTGTTCCATTCAATACCGTATTCATGGCAACAATCATTTCAG
CAGTAATCGGTACATTATGGATGGCAATTTTCGCTAACTATCCAATCGCGATTGCGCCAGGACTTGGTATGAACGCTTAC
TTCGTCACAGTAGTTACAAGTCAAAAACTAGATTATTCGGTCGCATTCGCTGCGGTCTTTGTAGCAGGTATTATTTTCTT
ATTATTATCTTTAACACCGCTTCGTGAAAAAATCATTGAAGCTATTCCACATAATTTAAAAGCTGGGATTACTGCTGGTA
TTGGTTTATTTATCGCTTTCCTTGGCTTCCGTATGACAGGCATTATTGTTTCTAACGATTCTAACTTAGTAGGTTTAGGT
GACTTACATTCCAAAGAAGCTATTTTAGCTATTGTTGGTCTTTTAATCACTTTAATCTTGCTTGCTTTAAACGTAAAAGG
CGCTTTATTTATTGGGATGATTGCAACTGGTATCATTGCTTTTATCACTGGTGAACTGAAATTTACAGAAGGTATCGTTA
AACTTCCGCCAATGCCAGAATTCGTCTTCACTAATCCTATTCACGCTTTTGGTGATGTGATGAGTTACGGGCTCTATGCT
GTCGTCCTGTCCTTCTTACTTATAACGATTTTTGATACAACCGGAACAATGATCGGTGTTGCAAAAAAAGCTGGCCTAAT
GAAAGGTGAGTCATTACCAAATGCGAAACAAGCCTTAATGGCTGATGCGGTTGCAACAAGTGTTGGTTCGATGTTTGGTA
CAACGCCAACAAGTGCTTACATTGAATCTTCTGCTGGGGTTGCAACTGGTGGACGTACTGGTCTTACTACACTTACAGTT
GCTATCCTGTTCATGGTATCAGCATTCTTCGCTCCACTTGTTGGTGCTGTCTCTGGAATTTCTGCTATTACTGCACCAGC
ACTTATAGTTGTTGGTAGTATGATGATTGGTGCTGTTAAAGAAATCGACTGGGATACATTAGATGAAGCATTCCCTGCTT
TCTTAGTTATTCTAGCTATGCCACTTACTTCAAGCATCGCAATCGGCTTAGCATTCGGATTTATTTCTTACCCAGTATTA
AAAGTATTCACTGGTAAATGGCGTGAACTTAACTGGTTCCTAATCGTTATCGCCGTATTATTCTTCATTCTCGTAGCATT
TTTACCACATTAA

Upstream 100 bases:

>100_bases
GTTGCTTTTTTAGTAAAAAACGAATAATATTGTATTTGTGGTTTTTAATGTTCGTTATTTACCATTAAGCATGATTTTAA
AAAAGGAGTGAAACACACGC

Downstream 100 bases:

>100_bases
TCAATGAATAAAAGCGTCTACTGAACTCAGCTAGGCGCTTTTTTATTGGACAAAAACCCGCTCGTTTGCTATAAAGAAGA
GAGAAACTAATTAGAAATGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MFQLKANGTDVKTEVISGFTTFLTMVYIVVVNPAILSAAGVPFNTVFMATIISAVIGTLWMAIFANYPIAIAPGLGMNAY
FVTVVTSQKLDYSVAFAAVFVAGIIFLLLSLTPLREKIIEAIPHNLKAGITAGIGLFIAFLGFRMTGIIVSNDSNLVGLG
DLHSKEAILAIVGLLITLILLALNVKGALFIGMIATGIIAFITGELKFTEGIVKLPPMPEFVFTNPIHAFGDVMSYGLYA
VVLSFLLITIFDTTGTMIGVAKKAGLMKGESLPNAKQALMADAVATSVGSMFGTTPTSAYIESSAGVATGGRTGLTTLTV
AILFMVSAFFAPLVGAVSGISAITAPALIVVGSMMIGAVKEIDWDTLDEAFPAFLVILAMPLTSSIAIGLAFGFISYPVL
KVFTGKWRELNWFLIVIAVLFFILVAFLPH

Sequences:

>Translated_430_residues
MFQLKANGTDVKTEVISGFTTFLTMVYIVVVNPAILSAAGVPFNTVFMATIISAVIGTLWMAIFANYPIAIAPGLGMNAY
FVTVVTSQKLDYSVAFAAVFVAGIIFLLLSLTPLREKIIEAIPHNLKAGITAGIGLFIAFLGFRMTGIIVSNDSNLVGLG
DLHSKEAILAIVGLLITLILLALNVKGALFIGMIATGIIAFITGELKFTEGIVKLPPMPEFVFTNPIHAFGDVMSYGLYA
VVLSFLLITIFDTTGTMIGVAKKAGLMKGESLPNAKQALMADAVATSVGSMFGTTPTSAYIESSAGVATGGRTGLTTLTV
AILFMVSAFFAPLVGAVSGISAITAPALIVVGSMMIGAVKEIDWDTLDEAFPAFLVILAMPLTSSIAIGLAFGFISYPVL
KVFTGKWRELNWFLIVIAVLFFILVAFLPH
>Mature_430_residues
MFQLKANGTDVKTEVISGFTTFLTMVYIVVVNPAILSAAGVPFNTVFMATIISAVIGTLWMAIFANYPIAIAPGLGMNAY
FVTVVTSQKLDYSVAFAAVFVAGIIFLLLSLTPLREKIIEAIPHNLKAGITAGIGLFIAFLGFRMTGIIVSNDSNLVGLG
DLHSKEAILAIVGLLITLILLALNVKGALFIGMIATGIIAFITGELKFTEGIVKLPPMPEFVFTNPIHAFGDVMSYGLYA
VVLSFLLITIFDTTGTMIGVAKKAGLMKGESLPNAKQALMADAVATSVGSMFGTTPTSAYIESSAGVATGGRTGLTTLTV
AILFMVSAFFAPLVGAVSGISAITAPALIVVGSMMIGAVKEIDWDTLDEAFPAFLVILAMPLTSSIAIGLAFGFISYPVL
KVFTGKWRELNWFLIVIAVLFFILVAFLPH

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=423, Percent_Identity=42.080378250591, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1790150, Length=431, Percent_Identity=42.92343387471, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790499, Length=426, Percent_Identity=34.037558685446, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI48994909, Length=420, Percent_Identity=32.6190476190476, Blast_Score=195, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45320; Mature: 45320

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLKANGTDVKTEVISGFTTFLTMVYIVVVNPAILSAAGVPFNTVFMATIISAVIGTLW
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MAIFANYPIAIAPGLGMNAYFVTVVTSQKLDYSVAFAAVFVAGIIFLLLSLTPLREKIIE
HHHHHCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AIPHNLKAGITAGIGLFIAFLGFRMTGIIVSNDSNLVGLGDLHSKEAILAIVGLLITLIL
HCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHH
LALNVKGALFIGMIATGIIAFITGELKFTEGIVKLPPMPEFVFTNPIHAFGDVMSYGLYA
HHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCEECCCCCCHHCCCCHHHHHHHHHHHHHH
VVLSFLLITIFDTTGTMIGVAKKAGLMKGESLPNAKQALMADAVATSVGSMFGTTPTSAY
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
IESSAGVATGGRTGLTTLTVAILFMVSAFFAPLVGAVSGISAITAPALIVVGSMMIGAVK
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWDTLDEAFPAFLVILAMPLTSSIAIGLAFGFISYPVLKVFTGKWRELNWFLIVIAVL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FFILVAFLPH
HHHHHHHCCC
>Mature Secondary Structure
MFQLKANGTDVKTEVISGFTTFLTMVYIVVVNPAILSAAGVPFNTVFMATIISAVIGTLW
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MAIFANYPIAIAPGLGMNAYFVTVVTSQKLDYSVAFAAVFVAGIIFLLLSLTPLREKIIE
HHHHHCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AIPHNLKAGITAGIGLFIAFLGFRMTGIIVSNDSNLVGLGDLHSKEAILAIVGLLITLIL
HCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHH
LALNVKGALFIGMIATGIIAFITGELKFTEGIVKLPPMPEFVFTNPIHAFGDVMSYGLYA
HHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCEECCCCCCHHCCCCHHHHHHHHHHHHHH
VVLSFLLITIFDTTGTMIGVAKKAGLMKGESLPNAKQALMADAVATSVGSMFGTTPTSAY
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
IESSAGVATGGRTGLTTLTVAILFMVSAFFAPLVGAVSGISAITAPALIVVGSMMIGAVK
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWDTLDEAFPAFLVILAMPLTSSIAIGLAFGFISYPVLKVFTGKWRELNWFLIVIAVL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FFILVAFLPH
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660 [H]