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The map label for this gene is fumC

Identifier: 226224833

GI number: 226224833

Start: 2320453

End: 2321820

Strand: Reverse

Name: fumC

Synonym: Lm4b_02252

Alternate gene names: 226224833

Gene position: 2321820-2320453 (Counterclockwise)

Preceding gene: 226224834

Following gene: 226224832

Centisome position: 79.71

GC content: 39.25

Gene sequence:

>1368_bases
ATGGAACGAATAGAGCGAGATACGCTTGGAGAAATTTCTGTAGATGCGACGAAATATTGGGGAGCGCAAACAGAACGAAG
TAAAAGGAACTTTGCTATTGGTGATAATCCTATGCCGATAGAAATTATATATGCTTTTGCTCAATTAAAGAAAGCCACTG
CAAAAGTAAACGCAGCAGAAGGAAAACTATCCGAAGAAAAAGCGATAGCGATTGGCCAGGTTTGTGATCAAATCATTCAA
GGAGAACTAGATGAGCATTTTCCTTTAGTCGTTTGGCAAACCGGAAGCGGAACGCAGAGCAACATGAATGTCAATGAAGT
TATCGCACATGTTGCCAACTTAACTTTAGGCGAAGGACAAATTCATCCGAATGATGATGTCAACATGTCGCAAAGCTCGA
ACGACACATTTCCAACAGCGATGCATATCGCTGCATACGGGGCTTTAGTAACAAAACTTTTACCTGAAATAACTAAAATG
GAAGCTGTTTTGGCAGAGAAGAAAAATAAATACATGCATCTCGTTAAAATTGGCAGAACACATTTGCAAGACGCAACCCC
ATTAACACTTGGCCAAGAAATTAGTGGCTGGGAAGCATGTCTTACAAATAATAAAAACTATCTTGAAACAAGTATGAAAG
CAATTTTGCCACTTGCGATTGGTGGGACGGCAGTTGGAACTGGCTTAAATGCCTCACGTGATTTTGGTGATAAGGTTGCC
GAAGAATTGATGAAACAAACGGGATATCCTTTTACTTCTGATTCCAATAAGTATTTTGCTCTAACTAGTCATAGTCCGAT
TAATTTTGTTCATGGATCCATTCGTAGCCTTGCTTCGGATCTAATGAAAATTGCTAATGATATCCGTTTATTAGCAAGTG
GACCACGTAGCGGCATAGGTGAGTTAACAATCCCAGCTAATGAACCAGGAAGCTCCATTATGCCAGGAAAAGTAAATCCT
ACTCAATGTGAAGCAATGACGATGGTAGCGGCTCAAGTCATGGGAAATGATGTGACAATTAATGTTGCCGCCAGCCAAGG
AAACTTTGAACTCAATGTCTACAAGCCAGTTATCATTTTTAATTTCTTAGAATCTGTCAAACTGCTTTCAGATAGCATGC
GTTCGTTCCGATTGCATTGTTTGGAAGGACTTACTGCGAATGAAAAAGTTATTGAAACAAAAGTAAATGACTCCCTAATG
CTTGTGACAGCATTGAATCCGCATATCGGCTATGAAAAAGCTGCCAAAATTGCCAAGCTGGCTTTTGATGAAAATACGAC
ATTAAAAGAAGCGGCAATCAAAACCGGTTTTGTGACAGAAAAACAATTTGATTTATGGATTGATCCGTTAAAAATGACTA
ATTTGTAA

Upstream 100 bases:

>100_bases
AAAAATAAAACCACTCCCAAAAACGGAATAGCGAACATTTTTCATGTGTTTTGCGAAAACATCCGCTTTATACTAAAATA
GAATAAAAAGGGGTGGCGAC

Downstream 100 bases:

>100_bases
TCTCATACTTTAGAATGAACTTCCCTAGAAACAATTGTTGTCTAGGGATTTTTCACGTATAATGAAACCACTTTTACTAG
ACAAATATTAGGAGATGCAA

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MERIERDTLGEISVDATKYWGAQTERSKRNFAIGDNPMPIEIIYAFAQLKKATAKVNAAEGKLSEEKAIAIGQVCDQIIQ
GELDEHFPLVVWQTGSGTQSNMNVNEVIAHVANLTLGEGQIHPNDDVNMSQSSNDTFPTAMHIAAYGALVTKLLPEITKM
EAVLAEKKNKYMHLVKIGRTHLQDATPLTLGQEISGWEACLTNNKNYLETSMKAILPLAIGGTAVGTGLNASRDFGDKVA
EELMKQTGYPFTSDSNKYFALTSHSPINFVHGSIRSLASDLMKIANDIRLLASGPRSGIGELTIPANEPGSSIMPGKVNP
TQCEAMTMVAAQVMGNDVTINVAASQGNFELNVYKPVIIFNFLESVKLLSDSMRSFRLHCLEGLTANEKVIETKVNDSLM
LVTALNPHIGYEKAAKIAKLAFDENTTLKEAAIKTGFVTEKQFDLWIDPLKMTNL

Sequences:

>Translated_455_residues
MERIERDTLGEISVDATKYWGAQTERSKRNFAIGDNPMPIEIIYAFAQLKKATAKVNAAEGKLSEEKAIAIGQVCDQIIQ
GELDEHFPLVVWQTGSGTQSNMNVNEVIAHVANLTLGEGQIHPNDDVNMSQSSNDTFPTAMHIAAYGALVTKLLPEITKM
EAVLAEKKNKYMHLVKIGRTHLQDATPLTLGQEISGWEACLTNNKNYLETSMKAILPLAIGGTAVGTGLNASRDFGDKVA
EELMKQTGYPFTSDSNKYFALTSHSPINFVHGSIRSLASDLMKIANDIRLLASGPRSGIGELTIPANEPGSSIMPGKVNP
TQCEAMTMVAAQVMGNDVTINVAASQGNFELNVYKPVIIFNFLESVKLLSDSMRSFRLHCLEGLTANEKVIETKVNDSLM
LVTALNPHIGYEKAAKIAKLAFDENTTLKEAAIKTGFVTEKQFDLWIDPLKMTNL
>Mature_455_residues
MERIERDTLGEISVDATKYWGAQTERSKRNFAIGDNPMPIEIIYAFAQLKKATAKVNAAEGKLSEEKAIAIGQVCDQIIQ
GELDEHFPLVVWQTGSGTQSNMNVNEVIAHVANLTLGEGQIHPNDDVNMSQSSNDTFPTAMHIAAYGALVTKLLPEITKM
EAVLAEKKNKYMHLVKIGRTHLQDATPLTLGQEISGWEACLTNNKNYLETSMKAILPLAIGGTAVGTGLNASRDFGDKVA
EELMKQTGYPFTSDSNKYFALTSHSPINFVHGSIRSLASDLMKIANDIRLLASGPRSGIGELTIPANEPGSSIMPGKVNP
TQCEAMTMVAAQVMGNDVTINVAASQGNFELNVYKPVIIFNFLESVKLLSDSMRSFRLHCLEGLTANEKVIETKVNDSLM
LVTALNPHIGYEKAAKIAKLAFDENTTLKEAAIKTGFVTEKQFDLWIDPLKMTNL

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=457, Percent_Identity=54.9234135667396, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI266458395, Length=73, Percent_Identity=53.4246575342466, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1787896, Length=455, Percent_Identity=56.2637362637363, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082375, Length=450, Percent_Identity=36, Blast_Score=290, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17553882, Length=457, Percent_Identity=55.5798687089716, Blast_Score=506, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI32565146, Length=324, Percent_Identity=54.9382716049383, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324993, Length=456, Percent_Identity=53.9473684210526, Blast_Score=483, Evalue=1e-137,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=53.2467532467532, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24640177, Length=458, Percent_Identity=52.6200873362445, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24640179, Length=458, Percent_Identity=52.6200873362445, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24662684, Length=459, Percent_Identity=51.8518518518518, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24583245, Length=460, Percent_Identity=45.6521739130435, Blast_Score=403, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 49634; Mature: 49634

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIERDTLGEISVDATKYWGAQTERSKRNFAIGDNPMPIEIIYAFAQLKKATAKVNAAE
CCCCCCCCCCCEEECHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
GKLSEEKAIAIGQVCDQIIQGELDEHFPLVVWQTGSGTQSNMNVNEVIAHVANLTLGEGQ
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCC
IHPNDDVNMSQSSNDTFPTAMHIAAYGALVTKLLPEITKMEAVLAEKKNKYMHLVKIGRT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHH
HLQDATPLTLGQEISGWEACLTNNKNYLETSMKAILPLAIGGTAVGTGLNASRDFGDKVA
HCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHH
EELMKQTGYPFTSDSNKYFALTSHSPINFVHGSIRSLASDLMKIANDIRLLASGPRSGIG
HHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
ELTIPANEPGSSIMPGKVNPTQCEAMTMVAAQVMGNDVTINVAASQGNFELNVYKPVIIF
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECHHHHH
NFLESVKLLSDSMRSFRLHCLEGLTANEKVIETKVNDSLMLVTALNPHIGYEKAAKIAKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHH
AFDENTTLKEAAIKTGFVTEKQFDLWIDPLKMTNL
HCCCCCCHHHHHHHHCCCCCCCCCEEECCEECCCC
>Mature Secondary Structure
MERIERDTLGEISVDATKYWGAQTERSKRNFAIGDNPMPIEIIYAFAQLKKATAKVNAAE
CCCCCCCCCCCEEECHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
GKLSEEKAIAIGQVCDQIIQGELDEHFPLVVWQTGSGTQSNMNVNEVIAHVANLTLGEGQ
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCC
IHPNDDVNMSQSSNDTFPTAMHIAAYGALVTKLLPEITKMEAVLAEKKNKYMHLVKIGRT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHH
HLQDATPLTLGQEISGWEACLTNNKNYLETSMKAILPLAIGGTAVGTGLNASRDFGDKVA
HCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHH
EELMKQTGYPFTSDSNKYFALTSHSPINFVHGSIRSLASDLMKIANDIRLLASGPRSGIG
HHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
ELTIPANEPGSSIMPGKVNPTQCEAMTMVAAQVMGNDVTINVAASQGNFELNVYKPVIIF
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECHHHHH
NFLESVKLLSDSMRSFRLHCLEGLTANEKVIETKVNDSLMLVTALNPHIGYEKAAKIAKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHH
AFDENTTLKEAAIKTGFVTEKQFDLWIDPLKMTNL
HCCCCCCHHHHHHHHCCCCCCCCCEEECCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]