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The map label for this gene is clpB

Identifier: 226224814

GI number: 226224814

Start: 2300499

End: 2303099

Strand: Reverse

Name: clpB

Synonym: Lm4b_02233

Alternate gene names: 226224814

Gene position: 2303099-2300499 (Counterclockwise)

Preceding gene: 226224815

Following gene: 226224813

Centisome position: 79.07

GC content: 37.37

Gene sequence:

>2601_bases
ATGGATTTACAAAAATTTACGCAACAAGTCCAGCAAACAATTGCGGACGCTCAAAATTTAGCAATTGCATCCGAACATCA
AGAAATCGACGTCGCTCATGTTTTTAAAGTGTTATTAACAGAGAGTGATTTTGCCAAACGTGTATATGATGTAGCAGAAG
TAGATATAGATGCACTTCAAAAAACAGTAGAAGATGCTTTAACAAAAATTCCTGTGGTCTCTGGAAGCGGTGTAAACTAT
GGTCAAGCAATGAGCCAAGCACTTTTTCAGTTGATGCGGGATGCCGAAAAAGAACAAAAACAATTAGACGATGATTTTGT
ATCAACAGAACATCTAATCTTAGCTGTCATGGATCAAAAATCAAATCCAATAACAATGAATCTAAAAAAACAACATAAAT
CTAAAAAACAAATTCAAGAAGCTATTTTAAAAATCAGAGGAGGGAAAAAAGTGACTTCTCAAAATGCAGAAGAAAACTAT
GAAGCCTTAACAAAATATGGACGTGATTTAGTCGCGGAAGTTAGAAGTGGTAAACTCGATCCAGTAATCGGACGCGATGC
AGAAATTCGTAACGTTATCCGAATTTTATCTAGAAAAACAAAAAATAATCCAGTTCTGATAGGTGAACCAGGCGTTGGTA
AAACAGCCATTGTCGAAGGTTTAGCACAACGAATCGTTCGAAAAGACGTTCCAGAAGGGCTGAAAGATAAAACGATTATC
TCGCTTGATATCGGTTCCCTTATTGCTGGAGCTAAATATCGCGGTGAATTTGAAGAACGTTTGAAAGCAGTACTTCAAGA
AGTGAAACAAAGTGACGGTCAAATTTTACTATTTATTGATGAAATTCATACGATTGTCGGCGCAGGTAAAACAGATGGCG
CAATGGACGCCGGAAATATGTTAAAACCGATGCTTGCCCGCGGTGAACTACACTGTATCGGCGCAACTACGTTAGATGAA
TACCGCCAATATATTGAAAAAGATGCCGCACTCGAAAGACGTTTCCAAAAAGTACTTGTCCCAGAACCAACAGTGGAAGA
TACAGTATCCATTTTACGTGGGTTAAAAGAACGCTTTGAAATTCATCATGGGGTAAATATTCATGATAATGCTTTAGTTG
CAGCAGCGAGTCTTTCTAATCGTTATATTACGGACCGCTTCTTACCAGATAAAGCAATCGACTTAGTAGATGAAGCATGC
GCAACTATTCGTGTCGAAATTGACTCGATGCCAAGTGAACTCGACGAAGTAACGAGAAAAGTAATGCAATTAGAAATTGA
GGAAGCGGCTTTAAAAGAAGAAAAAGATCCAGCGAGTGAACGCCGTTTAGAAATATTGCAACGCGAACTAGCTGACTACA
AAGAAGAAGCCAATCAAATGAAATCGAAATGGGAATCTGAGAAAAACGAAATTAGTAAAATTCGTGAGGTCCGAGAACAA
ATCGATCATTTGCGTCATGAATTAGAAGAAGCAGAAAATAATTATGATTTAAATAAAGCAGCAGAATTACGACACGGTAA
AATCCCGGCTGTGGAAAAAGAATTACTAGAGTTAGAAGCAGAAAATCGCGAAAAAACTGCACAAGAAGACCGGATTTTAC
AAGAAGAAGTAACAGAAAATGAAATTGCAGAAATCGTCGGACGTTGGACAGGAATTCCAGTGACCAAGTTAGTAGAAGGA
GAACGCGAAAAACTGCTTAAATTAGCAGATGAACTTCATCAAAAAGTAATTGGTCAAGATGACGCTGTTCAATTAGTTAG
TGATGCTGTACTTCGTGCACGCGCTGGTATTAAAGATCCGAAACGCCCAATTGGCTCATTCATTTTCCTAGGGCCAACAG
GTGTTGGTAAAACAGAACTTGCCAAAGCACTCGCATTTAATATGTTTGATTCAGAAGATCATATGATTCGAATTGATATG
TCTGAATACATGGAGAAACATTCCGTATCAAGACTTGTCGGAGCTCCTCCAGGCTATGTTGGCTATGAAGAAGGCGGTCA
ACTTACTGAAGCTGTAAGACGAAATCCTTTTTCAATCGTCCTACTTGATGAAATAGAAAAAGCTCATCCAGATGTATTTA
ACATACTATTACAAGTACTTGATGATGGACGTATCACAGATTCACAAGGTCGATTAATTGATTTTAAAAATACAGTAATT
ATTATGACGTCAAATATTGGCTCTAATTTATTACTTGAAAGAACCGAAGAAGGCGAAATTTCACCGGAATTAGAATCGGA
TGTAATGCAAATTTTACAAAGTGAATTCAAACCGGAATTCTTAAACCGCGTAGATGATATTATTCTATTTAAACCACTAA
CACTAGCTGATATTAAAGGCATTGTGGAGAAATTAGTAGAAGAACTTCAAATCCGTTTAGCCGACCAAGAAATTACGATT
ACTATTTCCGATAATGCAAAAGCATTTATTGCGGAAGAAGCATATGATCCGGTATATGGCGCACGACCACTTAAACGCTA
TATTGTGCGTCATGTTGAAACGCCACTTGCTAGAGAAATTGTATCAGGTAAAATCATGCCACATTCATCTGTAGAAATTG
ATTTAGCAGATAAAGAATTTACTTTTAAAGTCACAGAATAA

Upstream 100 bases:

>100_bases
TAAAAAAGATGGTTTTTGTTAGACAAAAGCAAGATAAAAAACTATAATAAAGTTAAGGTCAAAAAAGGTCAGACTTATCT
TTTATAAGGAGGACGAATGA

Downstream 100 bases:

>100_bases
CATAGAAAAGGTTTGACACCTCGCTTGAAAGGGAAAACTTTGGTTGGATTAGTCGTTTAGAAAATAGACGAAAATGGAAG
GATGAGGAAGATGAAATTAG

Product: endopeptidase Clp ATP-binding chain B (ClpB)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 866; Mature: 866

Protein sequence:

>866_residues
MDLQKFTQQVQQTIADAQNLAIASEHQEIDVAHVFKVLLTESDFAKRVYDVAEVDIDALQKTVEDALTKIPVVSGSGVNY
GQAMSQALFQLMRDAEKEQKQLDDDFVSTEHLILAVMDQKSNPITMNLKKQHKSKKQIQEAILKIRGGKKVTSQNAEENY
EALTKYGRDLVAEVRSGKLDPVIGRDAEIRNVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRKDVPEGLKDKTII
SLDIGSLIAGAKYRGEFEERLKAVLQEVKQSDGQILLFIDEIHTIVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLDE
YRQYIEKDAALERRFQKVLVPEPTVEDTVSILRGLKERFEIHHGVNIHDNALVAAASLSNRYITDRFLPDKAIDLVDEAC
ATIRVEIDSMPSELDEVTRKVMQLEIEEAALKEEKDPASERRLEILQRELADYKEEANQMKSKWESEKNEISKIREVREQ
IDHLRHELEEAENNYDLNKAAELRHGKIPAVEKELLELEAENREKTAQEDRILQEEVTENEIAEIVGRWTGIPVTKLVEG
EREKLLKLADELHQKVIGQDDAVQLVSDAVLRARAGIKDPKRPIGSFIFLGPTGVGKTELAKALAFNMFDSEDHMIRIDM
SEYMEKHSVSRLVGAPPGYVGYEEGGQLTEAVRRNPFSIVLLDEIEKAHPDVFNILLQVLDDGRITDSQGRLIDFKNTVI
IMTSNIGSNLLLERTEEGEISPELESDVMQILQSEFKPEFLNRVDDIILFKPLTLADIKGIVEKLVEELQIRLADQEITI
TISDNAKAFIAEEAYDPVYGARPLKRYIVRHVETPLAREIVSGKIMPHSSVEIDLADKEFTFKVTE

Sequences:

>Translated_866_residues
MDLQKFTQQVQQTIADAQNLAIASEHQEIDVAHVFKVLLTESDFAKRVYDVAEVDIDALQKTVEDALTKIPVVSGSGVNY
GQAMSQALFQLMRDAEKEQKQLDDDFVSTEHLILAVMDQKSNPITMNLKKQHKSKKQIQEAILKIRGGKKVTSQNAEENY
EALTKYGRDLVAEVRSGKLDPVIGRDAEIRNVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRKDVPEGLKDKTII
SLDIGSLIAGAKYRGEFEERLKAVLQEVKQSDGQILLFIDEIHTIVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLDE
YRQYIEKDAALERRFQKVLVPEPTVEDTVSILRGLKERFEIHHGVNIHDNALVAAASLSNRYITDRFLPDKAIDLVDEAC
ATIRVEIDSMPSELDEVTRKVMQLEIEEAALKEEKDPASERRLEILQRELADYKEEANQMKSKWESEKNEISKIREVREQ
IDHLRHELEEAENNYDLNKAAELRHGKIPAVEKELLELEAENREKTAQEDRILQEEVTENEIAEIVGRWTGIPVTKLVEG
EREKLLKLADELHQKVIGQDDAVQLVSDAVLRARAGIKDPKRPIGSFIFLGPTGVGKTELAKALAFNMFDSEDHMIRIDM
SEYMEKHSVSRLVGAPPGYVGYEEGGQLTEAVRRNPFSIVLLDEIEKAHPDVFNILLQVLDDGRITDSQGRLIDFKNTVI
IMTSNIGSNLLLERTEEGEISPELESDVMQILQSEFKPEFLNRVDDIILFKPLTLADIKGIVEKLVEELQIRLADQEITI
TISDNAKAFIAEEAYDPVYGARPLKRYIVRHVETPLAREIVSGKIMPHSSVEIDLADKEFTFKVTE
>Mature_866_residues
MDLQKFTQQVQQTIADAQNLAIASEHQEIDVAHVFKVLLTESDFAKRVYDVAEVDIDALQKTVEDALTKIPVVSGSGVNY
GQAMSQALFQLMRDAEKEQKQLDDDFVSTEHLILAVMDQKSNPITMNLKKQHKSKKQIQEAILKIRGGKKVTSQNAEENY
EALTKYGRDLVAEVRSGKLDPVIGRDAEIRNVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRKDVPEGLKDKTII
SLDIGSLIAGAKYRGEFEERLKAVLQEVKQSDGQILLFIDEIHTIVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLDE
YRQYIEKDAALERRFQKVLVPEPTVEDTVSILRGLKERFEIHHGVNIHDNALVAAASLSNRYITDRFLPDKAIDLVDEAC
ATIRVEIDSMPSELDEVTRKVMQLEIEEAALKEEKDPASERRLEILQRELADYKEEANQMKSKWESEKNEISKIREVREQ
IDHLRHELEEAENNYDLNKAAELRHGKIPAVEKELLELEAENREKTAQEDRILQEEVTENEIAEIVGRWTGIPVTKLVEG
EREKLLKLADELHQKVIGQDDAVQLVSDAVLRARAGIKDPKRPIGSFIFLGPTGVGKTELAKALAFNMFDSEDHMIRIDM
SEYMEKHSVSRLVGAPPGYVGYEEGGQLTEAVRRNPFSIVLLDEIEKAHPDVFNILLQVLDDGRITDSQGRLIDFKNTVI
IMTSNIGSNLLLERTEEGEISPELESDVMQILQSEFKPEFLNRVDDIILFKPLTLADIKGIVEKLVEELQIRLADQEITI
TISDNAKAFIAEEAYDPVYGARPLKRYIVRHVETPLAREIVSGKIMPHSSVEIDLADKEFTFKVTE

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=328, Percent_Identity=35.0609756097561, Blast_Score=194, Evalue=4e-49,
Organism=Escherichia coli, GI1788943, Length=856, Percent_Identity=53.6214953271028, Blast_Score=933, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=323, Percent_Identity=44.8916408668731, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=709, Percent_Identity=51.3399153737659, Blast_Score=749, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=868, Percent_Identity=41.4746543778802, Blast_Score=656, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 97512; Mature: 97512

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLQKFTQQVQQTIADAQNLAIASEHQEIDVAHVFKVLLTESDFAKRVYDVAEVDIDALQ
CCHHHHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KTVEDALTKIPVVSGSGVNYGQAMSQALFQLMRDAEKEQKQLDDDFVSTEHLILAVMDQK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHEEEEECCC
SNPITMNLKKQHKSKKQIQEAILKIRGGKKVTSQNAEENYEALTKYGRDLVAEVRSGKLD
CCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PVIGRDAEIRNVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRKDVPEGLKDKTII
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
SLDIGSLIAGAKYRGEFEERLKAVLQEVKQSDGQILLFIDEIHTIVGAGKTDGAMDAGNM
EEEHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCHHH
LKPMLARGELHCIGATTLDEYRQYIEKDAALERRFQKVLVPEPTVEDTVSILRGLKERFE
HHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
IHHGVNIHDNALVAAASLSNRYITDRFLPDKAIDLVDEACATIRVEIDSMPSELDEVTRK
HHCCCCCCCCHHEEHHHHCCCCHHHHCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHH
VMQLEIEEAALKEEKDPASERRLEILQRELADYKEEANQMKSKWESEKNEISKIREVREQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDHLRHELEEAENNYDLNKAAELRHGKIPAVEKELLELEAENREKTAQEDRILQEEVTEN
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAEIVGRWTGIPVTKLVEGEREKLLKLADELHQKVIGQDDAVQLVSDAVLRARAGIKDP
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
KRPIGSFIFLGPTGVGKTELAKALAFNMFDSEDHMIRIDMSEYMEKHSVSRLVGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGQLTEAVRRNPFSIVLLDEIEKAHPDVFNILLQVLDDGRITDSQGRLIDFKNTVI
CCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEE
IMTSNIGSNLLLERTEEGEISPELESDVMQILQSEFKPEFLNRVDDIILFKPLTLADIKG
EEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
IVEKLVEELQIRLADQEITITISDNAKAFIAEEAYDPVYGARPLKRYIVRHVETPLAREI
HHHHHHHHHHHHCCCCEEEEEECCCCCEEEEHHCCCCCCCCCHHHHHHHHHHCCHHHHHH
VSGKIMPHSSVEIDLADKEFTFKVTE
HCCCCCCCCCEEEEECCCEEEEEECC
>Mature Secondary Structure
MDLQKFTQQVQQTIADAQNLAIASEHQEIDVAHVFKVLLTESDFAKRVYDVAEVDIDALQ
CCHHHHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KTVEDALTKIPVVSGSGVNYGQAMSQALFQLMRDAEKEQKQLDDDFVSTEHLILAVMDQK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHEEEEECCC
SNPITMNLKKQHKSKKQIQEAILKIRGGKKVTSQNAEENYEALTKYGRDLVAEVRSGKLD
CCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PVIGRDAEIRNVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRKDVPEGLKDKTII
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
SLDIGSLIAGAKYRGEFEERLKAVLQEVKQSDGQILLFIDEIHTIVGAGKTDGAMDAGNM
EEEHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCHHH
LKPMLARGELHCIGATTLDEYRQYIEKDAALERRFQKVLVPEPTVEDTVSILRGLKERFE
HHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
IHHGVNIHDNALVAAASLSNRYITDRFLPDKAIDLVDEACATIRVEIDSMPSELDEVTRK
HHCCCCCCCCHHEEHHHHCCCCHHHHCCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHH
VMQLEIEEAALKEEKDPASERRLEILQRELADYKEEANQMKSKWESEKNEISKIREVREQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDHLRHELEEAENNYDLNKAAELRHGKIPAVEKELLELEAENREKTAQEDRILQEEVTEN
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIAEIVGRWTGIPVTKLVEGEREKLLKLADELHQKVIGQDDAVQLVSDAVLRARAGIKDP
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
KRPIGSFIFLGPTGVGKTELAKALAFNMFDSEDHMIRIDMSEYMEKHSVSRLVGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGQLTEAVRRNPFSIVLLDEIEKAHPDVFNILLQVLDDGRITDSQGRLIDFKNTVI
CCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEE
IMTSNIGSNLLLERTEEGEISPELESDVMQILQSEFKPEFLNRVDDIILFKPLTLADIKG
EEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
IVEKLVEELQIRLADQEITITISDNAKAFIAEEAYDPVYGARPLKRYIVRHVETPLAREI
HHHHHHHHHHHHCCCCEEEEEECCCCCEEEEHHCCCCCCCCCHHHHHHHHHHCCHHHHHH
VSGKIMPHSSVEIDLADKEFTFKVTE
HCCCCCCCCCEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]