Click here to switch to the map view.

The map label for this gene is betL

Identifier: 226224698

GI number: 226224698

Start: 2171704

End: 2173227

Strand: Direct

Name: betL

Synonym: Lm4b_02113

Alternate gene names: 226224698

Gene position: 2171704-2173227 (Clockwise)

Preceding gene: 226224697

Following gene: 226224706

Centisome position: 74.56

GC content: 40.81

Gene sequence:

>1524_bases
TTGAAAAAATTAACAAGTGTTTTTTGGGGATCGGGTTGTCTTGTTTTATTAGCCGTTTTATTTGGAGCCTTTTTGCCAAT
ACAATTTGAAGCTTTTACAACCAATATCCAAAAATTTCTAACAAGTAATTTTGGTTGGTACTATTTAATTGTTGTAGCGA
TTATTATTATCTTCTGCTTGTTTTTAGTTTTAAGTCCGATTGGCTCGATTCGCCTCGGAAAACCTGGTGAGGAACCGGGG
TATAGTAATAAGTCTTGGTTTGCGATGTTGTTTAGTGCTGGAATGGGAATTGGCCTCGTTTTCTGGGGTGCAGCTGAGCC
GTTATCTCATTATGCGGTCCAAGCTCCTGGCGGTGAAGTCGGCACGCAAGCAGCGATGAAAGATGCGCTTCGCTATTCAT
TCTTCCACTGGGGAATTTCTGCTTGGTCGATTTATGCGATTGTCGCTTTAGCACTGGCTTACTTCAAATTCCGGAAAAAT
GCGCCGGGTTTAATAAGTGCCACTCTTTATCCTATTTTAGGGAAACATGCCAAAGGTCCAATTGGACAATTGATTGATAT
CATCGCTGTTTTTGCGACAGTCATCGGTGTTGCAACGACACTTGGCCTTGGCGCTCAACAAATTAATGGTGGTCTTACAT
ACTTGTTTGGCGTTCCAAACAATTTTACTGTCCAATTTACCATTATTATCATTGTCACCATTTTATTTATGTTATCGGCC
ATGTCTGGACTTGATAAAGGGATTCAGCTTTTAAGTAATGTAAATATTTATGTTGCTGGAGTTTTATTAGTTTTAACCCT
TATTCTTGGGCCTACCCTTTTCATTATGAATAACTTCACCAATTCATTTGGTGACTACTTACAAAATATCATCCAAATGA
GTTTTCAGACAGCCCCTGATGCGCCTGATGCTCGAAAATGGATTGACTCATGGACTATTTTTTATTGGGCTTGGTGGCTT
TCTTGGTCACCGTTCGTTGGGATTTTTATTGCCAGAATTTCGCGCGGTAGAACGATTCGCCAATTCTTGCTCGGTGTCAT
CGTGTTGCCCGCTTTAGTCAGTGTGTTTTGGTTTGCCGTATTTGGTGGCTCGGCGATTTTTGTCGAACAACATGGAAATT
CTGGGCTTTCAAGTTTAGCAACTGAACAAGTACTCTTTGGGGTATTTAATGAATTCCCAGCTGGCATGGTGTTATCGATT
GTTGCGATGATTTTAATTGCAGTCTTCTTTATTACTTCAGCAGACTCAGCCACATTTGTTCTCGGTATGCAAACGACGGG
CGGATCTTTAAATCCACCAAACTCCGTCAAAGTAACATGGGGCCTACTCCAAGCAGGAATAGCAAGTGTGCTACTTTATG
CAGGAGGACTCACAGCGCTTCAAAACGCATCGATTATAGCAGCCTTTCCATTTTCTATCGTCATTATCTTAATGATTGTT
TCGTTATTCGTTTCGTTAACGAGGGAACAAGAAAAACTCGGCTTATATGTCCGACCGAAAAAATCACAACGTTCTCAACT
ATAA

Upstream 100 bases:

>100_bases
ATTTGTCAGAGCACTACTTTTTTTGTTACTATTAAAAAGAATGTTACCTTTTTGCTAACATGGGGAAATAGATACAGAGA
ATATAAAGGGAAGTGATGTA

Downstream 100 bases:

>100_bases
TAAAAAGGGATGAAAATCGACAACTAGATTTTCATCCCTTTTTTCATCCTTTTTTCTGCTTTGCCGCGACTATTTCGCGA
TAGCTACGAGTTTGTTTAGG

Product: glycine betaine transporter BetL

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKKLTSVFWGSGCLVLLAVLFGAFLPIQFEAFTTNIQKFLTSNFGWYYLIVVAIIIIFCLFLVLSPIGSIRLGKPGEEPG
YSNKSWFAMLFSAGMGIGLVFWGAAEPLSHYAVQAPGGEVGTQAAMKDALRYSFFHWGISAWSIYAIVALALAYFKFRKN
APGLISATLYPILGKHAKGPIGQLIDIIAVFATVIGVATTLGLGAQQINGGLTYLFGVPNNFTVQFTIIIIVTILFMLSA
MSGLDKGIQLLSNVNIYVAGVLLVLTLILGPTLFIMNNFTNSFGDYLQNIIQMSFQTAPDAPDARKWIDSWTIFYWAWWL
SWSPFVGIFIARISRGRTIRQFLLGVIVLPALVSVFWFAVFGGSAIFVEQHGNSGLSSLATEQVLFGVFNEFPAGMVLSI
VAMILIAVFFITSADSATFVLGMQTTGGSLNPPNSVKVTWGLLQAGIASVLLYAGGLTALQNASIIAAFPFSIVIILMIV
SLFVSLTREQEKLGLYVRPKKSQRSQL

Sequences:

>Translated_507_residues
MKKLTSVFWGSGCLVLLAVLFGAFLPIQFEAFTTNIQKFLTSNFGWYYLIVVAIIIIFCLFLVLSPIGSIRLGKPGEEPG
YSNKSWFAMLFSAGMGIGLVFWGAAEPLSHYAVQAPGGEVGTQAAMKDALRYSFFHWGISAWSIYAIVALALAYFKFRKN
APGLISATLYPILGKHAKGPIGQLIDIIAVFATVIGVATTLGLGAQQINGGLTYLFGVPNNFTVQFTIIIIVTILFMLSA
MSGLDKGIQLLSNVNIYVAGVLLVLTLILGPTLFIMNNFTNSFGDYLQNIIQMSFQTAPDAPDARKWIDSWTIFYWAWWL
SWSPFVGIFIARISRGRTIRQFLLGVIVLPALVSVFWFAVFGGSAIFVEQHGNSGLSSLATEQVLFGVFNEFPAGMVLSI
VAMILIAVFFITSADSATFVLGMQTTGGSLNPPNSVKVTWGLLQAGIASVLLYAGGLTALQNASIIAAFPFSIVIILMIV
SLFVSLTREQEKLGLYVRPKKSQRSQL
>Mature_507_residues
MKKLTSVFWGSGCLVLLAVLFGAFLPIQFEAFTTNIQKFLTSNFGWYYLIVVAIIIIFCLFLVLSPIGSIRLGKPGEEPG
YSNKSWFAMLFSAGMGIGLVFWGAAEPLSHYAVQAPGGEVGTQAAMKDALRYSFFHWGISAWSIYAIVALALAYFKFRKN
APGLISATLYPILGKHAKGPIGQLIDIIAVFATVIGVATTLGLGAQQINGGLTYLFGVPNNFTVQFTIIIIVTILFMLSA
MSGLDKGIQLLSNVNIYVAGVLLVLTLILGPTLFIMNNFTNSFGDYLQNIIQMSFQTAPDAPDARKWIDSWTIFYWAWWL
SWSPFVGIFIARISRGRTIRQFLLGVIVLPALVSVFWFAVFGGSAIFVEQHGNSGLSSLATEQVLFGVFNEFPAGMVLSI
VAMILIAVFFITSADSATFVLGMQTTGGSLNPPNSVKVTWGLLQAGIASVLLYAGGLTALQNASIIAAFPFSIVIILMIV
SLFVSLTREQEKLGLYVRPKKSQRSQL

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=501, Percent_Identity=38.9221556886228, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1788102, Length=444, Percent_Identity=33.3333333333333, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1786224, Length=465, Percent_Identity=31.1827956989247, Blast_Score=205, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 55117; Mature: 55117

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCHHHCCEEECCCHHCCCCC
>Mature Secondary Structure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HHHHHHHCCHHHCCEEECCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]