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The map label for this gene is malS [H]

Identifier: 226224519

GI number: 226224519

Start: 1976025

End: 1977668

Strand: Direct

Name: malS [H]

Synonym: Lm4b_01932

Alternate gene names: 226224519

Gene position: 1976025-1977668 (Clockwise)

Preceding gene: 226224518

Following gene: 226224520

Centisome position: 67.84

GC content: 41.79

Gene sequence:

>1644_bases
ATGACTGTAAAACCAGGTTTTGACTATATGAATAATCCATTATTAAATAAAGGAACCGCTTTTTCAAAAGAAGAAAGAGC
AAGCTATCAATTGGACGGATTACTGCCACCAATTATCGAAACAATTGAACAACAAGCCGTGCGCATCGAGACGCAAATAG
AAAATTTAGAAACACCTTTACATAAACATCAGCTTTTAACAAACTTATATAATGAAAATCGCACCCTCTACTATTATGTC
GTAACAAAAAACGTGACTGACTATTTGCCAATTATTTATACACCAACTATCGGCGACGCAGTTATCCAGTACCATAAAGA
ATACACTGCGCCGGATGAAGCCTTATTTATTGATGCCTTTGCACCCGAAAAATTAAGCGCATCAATAAAAAACTACGCAA
AAAATAATCCGAATATTGATATGATTGTTATAACAGATGGTGAGGGTGTGCTTGGAATTGGCGACTGGGGTGTGAATGGT
GTGAAAATCGCTGTTGGAAAATTAGCGGTTTATACGGTTGCAGCTGGACTTGCACCAGACCGTGTTCTGCCAGTTGTTAT
TGACGCCGGAACGAACAACGAAACACTTCTTAACGATCCACTATATCTAGGAAATAAACGCCCCCGCCTTTCAGAAAGTG
AATATGACGCTTTTATTGCTTCTTTTGTTAATGTTATGAAAGAAGTTTTCCCGAAAGCCATCCTTCACTGGGAAGACTTT
GGACGCGCGAATGCAAGTCGTATTCTGCATAATTACCGCGATAAAATTTGTACATTTAACGATGATATTCAAGGAACTGG
GGCCATGGTGGTTGCTGCTGTTCTTGCGACGATCCAAGTTTCCAAAATACCATTAAGCGAGCAAAAGATTATTATTTTCG
GGGCTGGAACTGCTGGAATCGGTATCGCTGATCAACTTAGTGCTCAGTGGATGCGTGAAACTGGTCTTCCTTTTGAATCG
GCGAAAAAGCATTTTTATTTAGTCGACCGAAATGGTTTAGTGCTTGATAATATGACCGACTTAACTACCGGGCAAAAGAA
ATATGCACATCCGTCGACTGAGTGGTCTAACGTGCCCACAGATACGTTAGAAAACTTAATGGAAGCAGTTCATCCAACAA
TGTTAATCGGCTGTTCTGGTGTCACCGGTGCCTTTAAAGAGAGCATCGTCAAAAAAATGACCCAGTATACAGAACGACCA
GCGATTTTGCCACTTTCTAATCCAACAAAATTAGCCGAAGCAACTGCTTCTGATTTAATTCAGTGGACAGATGGAAAAGC
GCTTATTGTTACAGGTAGCCCTTCCAAACCAGTAGAATATCAACATACTACTTATGAAATCGGTCAAGCGAACAATGCAC
TTCTCTATCCGGGGCTCGGACTAGGTGCTCTTGTCACTCGTGCGAAATATATTACAGATGGTATGCTCGCTGCTGCTTCC
ATGGCTGTCGCTGAGCAGATTTCACCAAACAAAGCAGGCGCAGCCCTTCTACCACATGTGCGCACCCTTCGCGAAACATC
GCGTGCAGTAGCCATCGCTGTCGCTAACCAAGCTATTAAAGAAAACATCCATCAAGTCGAATTAACGAATGTCACCGAAG
CTATCGAGCGGGAAATGTGGCAACCTATTTATAAAGGAGTTTAG

Upstream 100 bases:

>100_bases
GAAAATCTGCTTATTGTCAGGTTTTCTTTTTGTTTGGAATCAGTTGTTATTCTTCTTTCCCCCATGTAAAATAGAATAGA
ATAAAAAGGAGTGCTGAAAA

Downstream 100 bases:

>100_bases
ATATGTTTTACAAAACGAAGCAATCACTAGATCAACTCGTCCAAAACGGCTCCACTCCGGGAATTAGTTATCAAATAAAA
ACTAATTATCTGGAAGAAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 3 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTVKPGFDYMNNPLLNKGTAFSKEERASYQLDGLLPPIIETIEQQAVRIETQIENLETPLHKHQLLTNLYNENRTLYYYV
VTKNVTDYLPIIYTPTIGDAVIQYHKEYTAPDEALFIDAFAPEKLSASIKNYAKNNPNIDMIVITDGEGVLGIGDWGVNG
VKIAVGKLAVYTVAAGLAPDRVLPVVIDAGTNNETLLNDPLYLGNKRPRLSESEYDAFIASFVNVMKEVFPKAILHWEDF
GRANASRILHNYRDKICTFNDDIQGTGAMVVAAVLATIQVSKIPLSEQKIIIFGAGTAGIGIADQLSAQWMRETGLPFES
AKKHFYLVDRNGLVLDNMTDLTTGQKKYAHPSTEWSNVPTDTLENLMEAVHPTMLIGCSGVTGAFKESIVKKMTQYTERP
AILPLSNPTKLAEATASDLIQWTDGKALIVTGSPSKPVEYQHTTYEIGQANNALLYPGLGLGALVTRAKYITDGMLAAAS
MAVAEQISPNKAGAALLPHVRTLRETSRAVAIAVANQAIKENIHQVELTNVTEAIEREMWQPIYKGV

Sequences:

>Translated_547_residues
MTVKPGFDYMNNPLLNKGTAFSKEERASYQLDGLLPPIIETIEQQAVRIETQIENLETPLHKHQLLTNLYNENRTLYYYV
VTKNVTDYLPIIYTPTIGDAVIQYHKEYTAPDEALFIDAFAPEKLSASIKNYAKNNPNIDMIVITDGEGVLGIGDWGVNG
VKIAVGKLAVYTVAAGLAPDRVLPVVIDAGTNNETLLNDPLYLGNKRPRLSESEYDAFIASFVNVMKEVFPKAILHWEDF
GRANASRILHNYRDKICTFNDDIQGTGAMVVAAVLATIQVSKIPLSEQKIIIFGAGTAGIGIADQLSAQWMRETGLPFES
AKKHFYLVDRNGLVLDNMTDLTTGQKKYAHPSTEWSNVPTDTLENLMEAVHPTMLIGCSGVTGAFKESIVKKMTQYTERP
AILPLSNPTKLAEATASDLIQWTDGKALIVTGSPSKPVEYQHTTYEIGQANNALLYPGLGLGALVTRAKYITDGMLAAAS
MAVAEQISPNKAGAALLPHVRTLRETSRAVAIAVANQAIKENIHQVELTNVTEAIEREMWQPIYKGV
>Mature_546_residues
TVKPGFDYMNNPLLNKGTAFSKEERASYQLDGLLPPIIETIEQQAVRIETQIENLETPLHKHQLLTNLYNENRTLYYYVV
TKNVTDYLPIIYTPTIGDAVIQYHKEYTAPDEALFIDAFAPEKLSASIKNYAKNNPNIDMIVITDGEGVLGIGDWGVNGV
KIAVGKLAVYTVAAGLAPDRVLPVVIDAGTNNETLLNDPLYLGNKRPRLSESEYDAFIASFVNVMKEVFPKAILHWEDFG
RANASRILHNYRDKICTFNDDIQGTGAMVVAAVLATIQVSKIPLSEQKIIIFGAGTAGIGIADQLSAQWMRETGLPFESA
KKHFYLVDRNGLVLDNMTDLTTGQKKYAHPSTEWSNVPTDTLENLMEAVHPTMLIGCSGVTGAFKESIVKKMTQYTERPA
ILPLSNPTKLAEATASDLIQWTDGKALIVTGSPSKPVEYQHTTYEIGQANNALLYPGLGLGALVTRAKYITDGMLAAASM
AVAEQISPNKAGAALLPHVRTLRETSRAVAIAVANQAIKENIHQVELTNVTEAIEREMWQPIYKGV

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=551, Percent_Identity=37.3865698729583, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI62420882, Length=523, Percent_Identity=37.2848948374761, Blast_Score=360, Evalue=3e-99,
Organism=Homo sapiens, GI62420880, Length=523, Percent_Identity=37.2848948374761, Blast_Score=360, Evalue=3e-99,
Organism=Homo sapiens, GI239049447, Length=523, Percent_Identity=37.2848948374761, Blast_Score=360, Evalue=3e-99,
Organism=Homo sapiens, GI4505145, Length=509, Percent_Identity=37.1316306483301, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI270265879, Length=452, Percent_Identity=38.716814159292, Blast_Score=318, Evalue=1e-86,
Organism=Escherichia coli, GI87081919, Length=541, Percent_Identity=41.9593345656192, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17537199, Length=528, Percent_Identity=39.5833333333333, Blast_Score=347, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6322823, Length=561, Percent_Identity=40.9982174688057, Blast_Score=463, Evalue=1e-131,
Organism=Drosophila melanogaster, GI21356279, Length=517, Percent_Identity=37.137330754352, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI281362672, Length=517, Percent_Identity=37.137330754352, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI281362674, Length=517, Percent_Identity=37.137330754352, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24646386, Length=547, Percent_Identity=34.7349177330896, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24646388, Length=547, Percent_Identity=34.7349177330896, Blast_Score=325, Evalue=6e-89,
Organism=Drosophila melanogaster, GI78707242, Length=536, Percent_Identity=33.7686567164179, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI78707238, Length=536, Percent_Identity=33.7686567164179, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI281363505, Length=547, Percent_Identity=31.4442413162706, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI78707236, Length=547, Percent_Identity=31.4442413162706, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI78707240, Length=516, Percent_Identity=34.3023255813954, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI281363503, Length=533, Percent_Identity=31.5196998123827, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI78707232, Length=519, Percent_Identity=32.3699421965318, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI19922384, Length=547, Percent_Identity=27.0566727605119, Blast_Score=224, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 60052; Mature: 59921

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKPGFDYMNNPLLNKGTAFSKEERASYQLDGLLPPIIETIEQQAVRIETQIENLETPL
CCCCCCCHHHCCCCCCCCCCCCCHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
HKHQLLTNLYNENRTLYYYVVTKNVTDYLPIIYTPTIGDAVIQYHKEYTAPDEALFIDAF
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECC
APEKLSASIKNYAKNNPNIDMIVITDGEGVLGIGDWGVNGVKIAVGKLAVYTVAAGLAPD
CCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHEEHHHHHCCCCC
RVLPVVIDAGTNNETLLNDPLYLGNKRPRLSESEYDAFIASFVNVMKEVFPKAILHWEDF
CEEEEEEECCCCCCEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRANASRILHNYRDKICTFNDDIQGTGAMVVAAVLATIQVSKIPLSEQKIIIFGAGTAGI
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
GIADQLSAQWMRETGLPFESAKKHFYLVDRNGLVLDNMTDLTTGQKKYAHPSTEWSNVPT
CCHHHHHHHHHHHHCCCHHHCCCEEEEEECCCEEECCCHHCCCCCHHCCCCCCCCCCCCH
DTLENLMEAVHPTMLIGCSGVTGAFKESIVKKMTQYTERPAILPLSNPTKLAEATASDLI
HHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHE
QWTDGKALIVTGSPSKPVEYQHTTYEIGQANNALLYPGLGLGALVTRAKYITDGMLAAAS
EECCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
MAVAEQISPNKAGAALLPHVRTLRETSRAVAIAVANQAIKENIHQVELTNVTEAIEREMW
HHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHCCHHEEEHHHHHHHHHHHHH
QPIYKGV
HHHHHCC
>Mature Secondary Structure 
TVKPGFDYMNNPLLNKGTAFSKEERASYQLDGLLPPIIETIEQQAVRIETQIENLETPL
CCCCCCHHHCCCCCCCCCCCCCHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
HKHQLLTNLYNENRTLYYYVVTKNVTDYLPIIYTPTIGDAVIQYHKEYTAPDEALFIDAF
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECC
APEKLSASIKNYAKNNPNIDMIVITDGEGVLGIGDWGVNGVKIAVGKLAVYTVAAGLAPD
CCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHEEHHHHHCCCCC
RVLPVVIDAGTNNETLLNDPLYLGNKRPRLSESEYDAFIASFVNVMKEVFPKAILHWEDF
CEEEEEEECCCCCCEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRANASRILHNYRDKICTFNDDIQGTGAMVVAAVLATIQVSKIPLSEQKIIIFGAGTAGI
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
GIADQLSAQWMRETGLPFESAKKHFYLVDRNGLVLDNMTDLTTGQKKYAHPSTEWSNVPT
CCHHHHHHHHHHHHCCCHHHCCCEEEEEECCCEEECCCHHCCCCCHHCCCCCCCCCCCCH
DTLENLMEAVHPTMLIGCSGVTGAFKESIVKKMTQYTERPAILPLSNPTKLAEATASDLI
HHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHE
QWTDGKALIVTGSPSKPVEYQHTTYEIGQANNALLYPGLGLGALVTRAKYITDGMLAAAS
EECCCCEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
MAVAEQISPNKAGAALLPHVRTLRETSRAVAIAVANQAIKENIHQVELTNVTEAIEREMW
HHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHCCHHEEEHHHHHHHHHHHHH
QPIYKGV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]