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The map label for this gene is lacF [H]

Identifier: 226224334

GI number: 226224334

Start: 1783415

End: 1784308

Strand: Direct

Name: lacF [H]

Synonym: Lm4b_01745

Alternate gene names: 226224334

Gene position: 1783415-1784308 (Clockwise)

Preceding gene: 226224333

Following gene: 226224335

Centisome position: 61.23

GC content: 37.7

Gene sequence:

>894_bases
ATGAAACAATTTTTAAATAAAAATACACCCTATTTGTTTATATCGCCAGCACTGTTTTTACTTCTTCTGTTTTCCCTACT
TCCGATTTTGCTTGCTTTTGTCATTAGTTTTACGGACATTGATTTAGTCGGGCTTGCCGATTACTCTAAAATTAACTTTG
TTGGTGTGGACAATTACGTCAATATTATGCAAGACCCGGTATTTTTAAAATCTATTTTTAACACGCTTTTCTATGTAATT
ATTGGCGTTCCACTTGTTATCGTCTGCTCGCTTGGTATTGCGCTGATGATTAACTTCTCACAAGCGAAGATTTTCCAATT
TTTCCGCTTAATCTTCTACACTCCTTCAATTACAAACGTTGTTGCTGTAGCTGTCGTTTGGAGTTACTTATATAATCCGC
GCTTTGGTTTACTTAACTATTTGCTCTCGTTTTTAGACTTAGGACCAGTTCCATGGTTACAAGATCCTACTATTGCGAAA
TTATCACTGATTATCCTAGCTGTTTGGCGGGCAATCGGTGTAAATATGATTATTTTCTTAGCCGCACTACAAGGCATTCC
ACGTGAATATTATGAAGCAGCCTCCCTTGACGGCGCAAATAGCCGCCAACAATTATTTAAAATTACGGTTCCAATGCTTC
GTTTCGCGATTTTCTTTGTAACAGTTACAACGATGATTGGTTGGTTGCAATTCTTCGAGGAGCCGTTCGTTATGACAGAA
GGTGGACCACTTGATAGTACGAACTCTGTCGCCCTCTTTATCTATCAAAACGGTTTCCAATTAAGTAAATTTGGTTATGC
CGCTGCTGGATCGTTCATCTTGTTTATCGCGATTATTATTATCACGATTATTCAATTCCGGATTCAGCGCAAAAATAATG
ATGGGGATATTTAA

Upstream 100 bases:

>100_bases
TACTTTCAACGATCAAGTAGAAGCACTTCTTAAAAAATAACGAATAAAAAGGGCAAATGATTTATTCTGCCATTTGCCCT
TTTAGAATGGACTGGATGTT

Downstream 100 bases:

>100_bases
GAGAGGAGTGTTTATAAATGAATCAATATAGACAAAAATCGCGCGGCGCTCAAATTGCCGTAATTACAATTTTAACGGTT
GGCGGATTCTTTATGATTTT

Product: sugar ABC transporter, permease protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKQFLNKNTPYLFISPALFLLLLFSLLPILLAFVISFTDIDLVGLADYSKINFVGVDNYVNIMQDPVFLKSIFNTLFYVI
IGVPLVIVCSLGIALMINFSQAKIFQFFRLIFYTPSITNVVAVAVVWSYLYNPRFGLLNYLLSFLDLGPVPWLQDPTIAK
LSLIILAVWRAIGVNMIIFLAALQGIPREYYEAASLDGANSRQQLFKITVPMLRFAIFFVTVTTMIGWLQFFEEPFVMTE
GGPLDSTNSVALFIYQNGFQLSKFGYAAAGSFILFIAIIIITIIQFRIQRKNNDGDI

Sequences:

>Translated_297_residues
MKQFLNKNTPYLFISPALFLLLLFSLLPILLAFVISFTDIDLVGLADYSKINFVGVDNYVNIMQDPVFLKSIFNTLFYVI
IGVPLVIVCSLGIALMINFSQAKIFQFFRLIFYTPSITNVVAVAVVWSYLYNPRFGLLNYLLSFLDLGPVPWLQDPTIAK
LSLIILAVWRAIGVNMIIFLAALQGIPREYYEAASLDGANSRQQLFKITVPMLRFAIFFVTVTTMIGWLQFFEEPFVMTE
GGPLDSTNSVALFIYQNGFQLSKFGYAAAGSFILFIAIIIITIIQFRIQRKNNDGDI
>Mature_297_residues
MKQFLNKNTPYLFISPALFLLLLFSLLPILLAFVISFTDIDLVGLADYSKINFVGVDNYVNIMQDPVFLKSIFNTLFYVI
IGVPLVIVCSLGIALMINFSQAKIFQFFRLIFYTPSITNVVAVAVVWSYLYNPRFGLLNYLLSFLDLGPVPWLQDPTIAK
LSLIILAVWRAIGVNMIIFLAALQGIPREYYEAASLDGANSRQQLFKITVPMLRFAIFFVTVTTMIGWLQFFEEPFVMTE
GGPLDSTNSVALFIYQNGFQLSKFGYAAAGSFILFIAIIIITIIQFRIQRKNNDGDI

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=289, Percent_Identity=29.757785467128, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787570, Length=252, Percent_Identity=31.7460317460317, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790465, Length=261, Percent_Identity=26.8199233716475, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33551; Mature: 33551

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFLNKNTPYLFISPALFLLLLFSLLPILLAFVISFTDIDLVGLADYSKINFVGVDNYV
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCHHH
NIMQDPVFLKSIFNTLFYVIIGVPLVIVCSLGIALMINFSQAKIFQFFRLIFYTPSITNV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHCCCHHHH
VAVAVVWSYLYNPRFGLLNYLLSFLDLGPVPWLQDPTIAKLSLIILAVWRAIGVNMIIFL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AALQGIPREYYEAASLDGANSRQQLFKITVPMLRFAIFFVTVTTMIGWLQFFEEPFVMTE
HHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
GGPLDSTNSVALFIYQNGFQLSKFGYAAAGSFILFIAIIIITIIQFRIQRKNNDGDI
CCCCCCCCCEEEEEEECCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKQFLNKNTPYLFISPALFLLLLFSLLPILLAFVISFTDIDLVGLADYSKINFVGVDNYV
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCHHH
NIMQDPVFLKSIFNTLFYVIIGVPLVIVCSLGIALMINFSQAKIFQFFRLIFYTPSITNV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHCCCHHHH
VAVAVVWSYLYNPRFGLLNYLLSFLDLGPVPWLQDPTIAKLSLIILAVWRAIGVNMIIFL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AALQGIPREYYEAASLDGANSRQQLFKITVPMLRFAIFFVTVTTMIGWLQFFEEPFVMTE
HHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
GGPLDSTNSVALFIYQNGFQLSKFGYAAAGSFILFIAIIIITIIQFRIQRKNNDGDI
CCCCCCCCCEEEEEEECCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]