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The map label for this gene is 226224270

Identifier: 226224270

GI number: 226224270

Start: 1708828

End: 1709739

Strand: Reverse

Name: 226224270

Synonym: Lm4b_01680

Alternate gene names: NA

Gene position: 1709739-1708828 (Counterclockwise)

Preceding gene: 226224271

Following gene: 226224269

Centisome position: 58.7

GC content: 40.79

Gene sequence:

>912_bases
TTGAAACGGAAGGTTGGAATAATTGGTACTGGTCATGTTGGGAGCGACGTGGCTTTTTCCCTCGTAACTCAAGGGATTTG
TGATGAAATTGTGTTAATAGATAAAATCGAAACTAAAGCAGAAAGTGAAGCGTTAGAACTGCGCGATATGGCTTCGATGA
CGAATTCGTATACTACAATAACTTCGAATGACTGGAGCGCACTAGGTGATGCCGATGTCATTGTCATGGCTGTTGGTCCA
GAAACGTTACTGCGCGAGGATCGTATGGAAGAGCTCGTTGAAACAAGTCGGTCAGTGGCTGAGATTGTACCAAAAATTAT
CGCATCGGGGTTTAAAGGGATTTTTGTGAATATTACGAATCCATGTGATGTGATTACGATGCTTATTCAGAAATTATCTG
GGTTTGATCATTCACGAGTATTTGGGACGGGAACATCACTTGATACAGCAAGAATGAGACGAGTAGTAGGAGAAGCATTA
CATATTAATCCAAAGAGTGTGGAAGGATACGTTCTTGGCGAGCATGGTGAATCACAATTCGTTGCTTGGTCTACGGTGAA
AATTGGCGGGGTAAGCATCACGGATTATAAAACAACAACACCACTTGATTTACCAGCATTAAAAGATGCAGTTCGTGGTG
GTGGTTGGAATATTTTGATGGGTAAAGGCTGGACGAGTTTTGGAATAGCCACAGCTGCAGCGGGGATTGTCGATGCGATT
TTAACAGATGCTAAACAAGTTTTTCCATTAGCTGTATTTTCCGAAAAAACAAATACGTACATCGGACAACCAGCAATCAT
TGGTGCAAATGGCGTGATAGATATTTTAGAACCGAAACTAACTACAGATGAAGTGGCTAATTTTAATGCATCAGCTGAAG
TTATTAAAACAGCTTTTCAGTTAATTGAATAA

Upstream 100 bases:

>100_bases
GAAGCAATCCCTCGAAAACTCGTGTGGGAAAATAATAAAACTATCATTAAATATGAATTAATCGCATTAAATTCAAGTAA
TTAATTGTGGAGGGGATTTT

Downstream 100 bases:

>100_bases
TTAGTTTGCCATGGAGAAAATAGGGGAATAAAATGGCAATAGCTTTTGAAATGGGGAAAACAAATGGGGGTTTATAAAAG
GGAGATGGGGATGAATGAAA

Product: L-2-hydroxyisocaproate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: L-HicDH [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKRKVGIIGTGHVGSDVAFSLVTQGICDEIVLIDKIETKAESEALELRDMASMTNSYTTITSNDWSALGDADVIVMAVGP
ETLLREDRMEELVETSRSVAEIVPKIIASGFKGIFVNITNPCDVITMLIQKLSGFDHSRVFGTGTSLDTARMRRVVGEAL
HINPKSVEGYVLGEHGESQFVAWSTVKIGGVSITDYKTTTPLDLPALKDAVRGGGWNILMGKGWTSFGIATAAAGIVDAI
LTDAKQVFPLAVFSEKTNTYIGQPAIIGANGVIDILEPKLTTDEVANFNASAEVIKTAFQLIE

Sequences:

>Translated_303_residues
MKRKVGIIGTGHVGSDVAFSLVTQGICDEIVLIDKIETKAESEALELRDMASMTNSYTTITSNDWSALGDADVIVMAVGP
ETLLREDRMEELVETSRSVAEIVPKIIASGFKGIFVNITNPCDVITMLIQKLSGFDHSRVFGTGTSLDTARMRRVVGEAL
HINPKSVEGYVLGEHGESQFVAWSTVKIGGVSITDYKTTTPLDLPALKDAVRGGGWNILMGKGWTSFGIATAAAGIVDAI
LTDAKQVFPLAVFSEKTNTYIGQPAIIGANGVIDILEPKLTTDEVANFNASAEVIKTAFQLIE
>Mature_303_residues
MKRKVGIIGTGHVGSDVAFSLVTQGICDEIVLIDKIETKAESEALELRDMASMTNSYTTITSNDWSALGDADVIVMAVGP
ETLLREDRMEELVETSRSVAEIVPKIIASGFKGIFVNITNPCDVITMLIQKLSGFDHSRVFGTGTSLDTARMRRVVGEAL
HINPKSVEGYVLGEHGESQFVAWSTVKIGGVSITDYKTTTPLDLPALKDAVRGGGWNILMGKGWTSFGIATAAAGIVDAI
LTDAKQVFPLAVFSEKTNTYIGQPAIIGANGVIDILEPKLTTDEVANFNASAEVIKTAFQLIE

Specific function: Catalyzes the NADP dependent reversible and stereospecific interconversion between 2-ketocarboxylic acids and L-2-hydroxy-carboxylic acids. 2-ketoacids with medium chain length (five to six C-atoms) are the best substrates [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=303, Percent_Identity=31.3531353135314, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI221136809, Length=303, Percent_Identity=31.3531353135314, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI15082234, Length=303, Percent_Identity=29.042904290429, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=28.2467532467532, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=28.2467532467532, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI291575128, Length=304, Percent_Identity=27.6315789473684, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI4557032, Length=304, Percent_Identity=27.6315789473684, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI9257228, Length=303, Percent_Identity=30.3630363036304, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI4504973, Length=303, Percent_Identity=30.3630363036304, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI260099725, Length=208, Percent_Identity=30.7692307692308, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI260099727, Length=208, Percent_Identity=30.7692307692308, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI207028494, Length=308, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=24.1157556270096, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI103472015, Length=183, Percent_Identity=25.6830601092896, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=290, Percent_Identity=27.5862068965517, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17136226, Length=303, Percent_Identity=28.3828382838284, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45550422, Length=303, Percent_Identity=26.4026402640264, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: 1.1.1.27

Molecular weight: Translated: 32425; Mature: 32425

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKVGIIGTGHVGSDVAFSLVTQGICDEIVLIDKIETKAESEALELRDMASMTNSYTTI
CCCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHCCEEEE
TSNDWSALGDADVIVMAVGPETLLREDRMEELVETSRSVAEIVPKIIASGFKGIFVNITN
ECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
PCDVITMLIQKLSGFDHSRVFGTGTSLDTARMRRVVGEALHINPKSVEGYVLGEHGESQF
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCE
VAWSTVKIGGVSITDYKTTTPLDLPALKDAVRGGGWNILMGKGWTSFGIATAAAGIVDAI
EEEEEEEECCEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
LTDAKQVFPLAVFSEKTNTYIGQPAIIGANGVIDILEPKLTTDEVANFNASAEVIKTAFQ
HHHHHHHHEEEEECCCCCCCCCCCEEECCCCCEEECCCCCCHHHHHCCCCHHHHHHHHHH
LIE
HCC
>Mature Secondary Structure
MKRKVGIIGTGHVGSDVAFSLVTQGICDEIVLIDKIETKAESEALELRDMASMTNSYTTI
CCCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHCCEEEE
TSNDWSALGDADVIVMAVGPETLLREDRMEELVETSRSVAEIVPKIIASGFKGIFVNITN
ECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
PCDVITMLIQKLSGFDHSRVFGTGTSLDTARMRRVVGEALHINPKSVEGYVLGEHGESQF
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCE
VAWSTVKIGGVSITDYKTTTPLDLPALKDAVRGGGWNILMGKGWTSFGIATAAAGIVDAI
EEEEEEEECCEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
LTDAKQVFPLAVFSEKTNTYIGQPAIIGANGVIDILEPKLTTDEVANFNASAEVIKTAFQ
HHHHHHHHEEEEECCCCCCCCCCCEEECCCCCEEECCCCCCHHHHHCCCCHHHHHHHHHH
LIE
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-lactate; NAD+

Specific reaction: (S)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684788; 7643402 [H]