Click here to switch to the map view.

The map label for this gene is yfeV [C]

Identifier: 226224260

GI number: 226224260

Start: 1691811

End: 1693259

Strand: Reverse

Name: yfeV [C]

Synonym: Lm4b_01670

Alternate gene names: 226224260

Gene position: 1693259-1691811 (Counterclockwise)

Preceding gene: 226224261

Following gene: 226224259

Centisome position: 58.13

GC content: 38.03

Gene sequence:

>1449_bases
ATGACAAAAGAAGCAGAAATTGGAAAACAAATATTTATTCATGTCGGTGGAATGGAAAACGTTTCAAGAATTGCACATTG
TATGACACGAGTTCGATTGGGGATAGTTGATAGTGACCTTGTTGATGTAGCTGGATTAAAAAAAGTTCCGGGTGTTATCG
GTGTTGTAGAAGATGATACTTTACAGATTATTGTTGGACCAGGAGTTGTGAACAAAGTGGCCGGCGCAATGGCAGAAATG
GCTGGTGTTAAAATTGGCGAAACGATTCAAGAAAATTTGGATAGCGGCACAAAAACTGGTAAGGAATTAGTCGAAGAAAA
AGCATCCAAAACAAAAGCTGAATTAAAAGCAAAACAAAATAATTCATCTGGTTTTAAAAGATTACTAAAATCAATCTCTA
ATATTTTTGTTCCACTAATTCCGGGTTTTGTTGGAGCGGGATTAATTGCTGGTATTGCAGCAATTATTTCTAATAATATA
ACAGCTGGAAATTTAGATGCTGCTGTTTGGACTCAATATATTGATATTTTAGGAGTTATTAATAAAGGAATATTTGCCTT
CTTAGCTATTTATGTAGGGATTAATACCGCGAATGAATTTGGTGGAACACCTGTACTCGGTGGTGGAATCGCTGGGATAA
CATTGCTTTCCGGATTAGCAGAAGGACATACAATTACAAACATTTTCACGGGGGATCCTATCGTAGCCGGACAGGGTGGC
ATTATCGGCGTATTATTAGCAGTTTGGTTAATGTGCGTACTTGAAAAAAACTTGCGCAAAATCATTCCGAATGCGATAGA
TATTATTTTTACGCCAACGCTTGTATTACTTATTATTGGTTTAGTAACTATTTTCTTAATTATGCCTTTTGCAGGACTTG
TATCAGATGGTTTAGTTAACGGTATTAATTGGGTAATCGAAGTTGGAGGAGTATTCGCAGGTTTTGTGCTAGGGACGTTA
TTCTTACCAATGGTTATGTTTGGGTTACATCAAGTTTTAACACCCATCCATGTAGAAATGATTGCTCAAAATGGTTATAC
TATCTTACTTCCTATTCTAGCTATGGCAGGTGGCGGACAAGTTGGTGCAGCAATCGCGCTTTGGATTCGTTGTCGTAAAA
ATAAACCACTTGTAAATATGATTAAAGGGGGTCTACCGGTTGGAATTTTAGGTATCGGTGAACCATTAATTTATGGGGTA
ACAATACCACTTGGGAAACCTTTTCTTACAGCTTGTCTCGGAGGCGGTATCGGTGGGGCAGTAATCGGCTACTTTGGTAA
CGTCGGAGCAATTGCAATCGGCCCATCAGGAGTTGCACTTATCCCACTAATCGCGAATAATGAATGGCTTTCTTACGTCA
TCGGATTAATTGCAGCATATTTAGGTGGATTCATTTTAACTTATTTCTTTGGTACACCAAAAGACGCAATGAATAGCGTA
GAATTGTAA

Upstream 100 bases:

>100_bases
CATGATCTTAACCAATTCAACCAAAGAAATAGCGTTAGAAAAATTAAGTGAAGCAAAAGGGTTTGTTCGAAACACCATTC
AAAAATAAGGAGCTGGAATA

Downstream 100 bases:

>100_bases
GAAGTAATTGCTGTTCCACGGAAATTTGTACACATTGAAGTGAATTGATTAGAGAAGCTTCGAAATTAGTTTTATTACTA
ATTTCGAAGCTTTTTTACGC

Product: sucrose-specific PTS system IIBC component

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTKEAEIGKQIFIHVGGMENVSRIAHCMTRVRLGIVDSDLVDVAGLKKVPGVIGVVEDDTLQIIVGPGVVNKVAGAMAEM
AGVKIGETIQENLDSGTKTGKELVEEKASKTKAELKAKQNNSSGFKRLLKSISNIFVPLIPGFVGAGLIAGIAAIISNNI
TAGNLDAAVWTQYIDILGVINKGIFAFLAIYVGINTANEFGGTPVLGGGIAGITLLSGLAEGHTITNIFTGDPIVAGQGG
IIGVLLAVWLMCVLEKNLRKIIPNAIDIIFTPTLVLLIIGLVTIFLIMPFAGLVSDGLVNGINWVIEVGGVFAGFVLGTL
FLPMVMFGLHQVLTPIHVEMIAQNGYTILLPILAMAGGGQVGAAIALWIRCRKNKPLVNMIKGGLPVGILGIGEPLIYGV
TIPLGKPFLTACLGGGIGGAVIGYFGNVGAIAIGPSGVALIPLIANNEWLSYVIGLIAAYLGGFILTYFFGTPKDAMNSV
EL

Sequences:

>Translated_482_residues
MTKEAEIGKQIFIHVGGMENVSRIAHCMTRVRLGIVDSDLVDVAGLKKVPGVIGVVEDDTLQIIVGPGVVNKVAGAMAEM
AGVKIGETIQENLDSGTKTGKELVEEKASKTKAELKAKQNNSSGFKRLLKSISNIFVPLIPGFVGAGLIAGIAAIISNNI
TAGNLDAAVWTQYIDILGVINKGIFAFLAIYVGINTANEFGGTPVLGGGIAGITLLSGLAEGHTITNIFTGDPIVAGQGG
IIGVLLAVWLMCVLEKNLRKIIPNAIDIIFTPTLVLLIIGLVTIFLIMPFAGLVSDGLVNGINWVIEVGGVFAGFVLGTL
FLPMVMFGLHQVLTPIHVEMIAQNGYTILLPILAMAGGGQVGAAIALWIRCRKNKPLVNMIKGGLPVGILGIGEPLIYGV
TIPLGKPFLTACLGGGIGGAVIGYFGNVGAIAIGPSGVALIPLIANNEWLSYVIGLIAAYLGGFILTYFFGTPKDAMNSV
EL
>Mature_481_residues
TKEAEIGKQIFIHVGGMENVSRIAHCMTRVRLGIVDSDLVDVAGLKKVPGVIGVVEDDTLQIIVGPGVVNKVAGAMAEMA
GVKIGETIQENLDSGTKTGKELVEEKASKTKAELKAKQNNSSGFKRLLKSISNIFVPLIPGFVGAGLIAGIAAIISNNIT
AGNLDAAVWTQYIDILGVINKGIFAFLAIYVGINTANEFGGTPVLGGGIAGITLLSGLAEGHTITNIFTGDPIVAGQGGI
IGVLLAVWLMCVLEKNLRKIIPNAIDIIFTPTLVLLIIGLVTIFLIMPFAGLVSDGLVNGINWVIEVGGVFAGFVLGTLF
LPMVMFGLHQVLTPIHVEMIAQNGYTILLPILAMAGGGQVGAAIALWIRCRKNKPLVNMIKGGLPVGILGIGEPLIYGVT
IPLGKPFLTACLGGGIGGAVIGYFGNVGAIAIGPSGVALIPLIANNEWLSYVIGLIAAYLGGFILTYFFGTPKDAMNSVE
L

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=494, Percent_Identity=37.8542510121458, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1790159, Length=435, Percent_Identity=29.4252873563218, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI2367362, Length=488, Percent_Identity=26.6393442622951, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994906, Length=484, Percent_Identity=26.8595041322314, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 50068; Mature: 49937

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEAEIGKQIFIHVGGMENVSRIAHCMTRVRLGIVDSDLVDVAGLKKVPGVIGVVEDDT
CCCCHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHCCCEEEEECCCC
LQIIVGPGVVNKVAGAMAEMAGVKIGETIQENLDSGTKTGKELVEEKASKTKAELKAKQN
EEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
NSSGFKRLLKSISNIFVPLIPGFVGAGLIAGIAAIISNNITAGNLDAAVWTQYIDILGVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
NKGIFAFLAIYVGINTANEFGGTPVLGGGIAGITLLSGLAEGHTITNIFTGDPIVAGQGG
HHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEECCCC
IIGVLLAVWLMCVLEKNLRKIIPNAIDIIFTPTLVLLIIGLVTIFLIMPFAGLVSDGLVN
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GINWVIEVGGVFAGFVLGTLFLPMVMFGLHQVLTPIHVEMIAQNGYTILLPILAMAGGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCC
VGAAIALWIRCRKNKPLVNMIKGGLPVGILGIGEPLIYGVTIPLGKPFLTACLGGGIGGA
HHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHHHHHCCCHHHH
VIGYFGNVGAIAIGPSGVALIPLIANNEWLSYVIGLIAAYLGGFILTYFFGTPKDAMNSV
HHHHHCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
EL
CC
>Mature Secondary Structure 
TKEAEIGKQIFIHVGGMENVSRIAHCMTRVRLGIVDSDLVDVAGLKKVPGVIGVVEDDT
CCCHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHCCCEEEEECCCC
LQIIVGPGVVNKVAGAMAEMAGVKIGETIQENLDSGTKTGKELVEEKASKTKAELKAKQN
EEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
NSSGFKRLLKSISNIFVPLIPGFVGAGLIAGIAAIISNNITAGNLDAAVWTQYIDILGVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
NKGIFAFLAIYVGINTANEFGGTPVLGGGIAGITLLSGLAEGHTITNIFTGDPIVAGQGG
HHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEECCCC
IIGVLLAVWLMCVLEKNLRKIIPNAIDIIFTPTLVLLIIGLVTIFLIMPFAGLVSDGLVN
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GINWVIEVGGVFAGFVLGTLFLPMVMFGLHQVLTPIHVEMIAQNGYTILLPILAMAGGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCC
VGAAIALWIRCRKNKPLVNMIKGGLPVGILGIGEPLIYGVTIPLGKPFLTACLGGGIGGA
HHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHHHHHCCCHHHH
VIGYFGNVGAIAIGPSGVALIPLIANNEWLSYVIGLIAAYLGGFILTYFFGTPKDAMNSV
HHHHHCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
EL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA