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The map label for this gene is yhcA [H]

Identifier: 226224218

GI number: 226224218

Start: 1643171

End: 1644640

Strand: Reverse

Name: yhcA [H]

Synonym: Lm4b_01628

Alternate gene names: 226224218

Gene position: 1644640-1643171 (Counterclockwise)

Preceding gene: 226224219

Following gene: 226224217

Centisome position: 56.46

GC content: 40.27

Gene sequence:

>1470_bases
TTGAATATGAAAGCAGCAAGTACATCAGTGAAGCGTAATGGTATTTTGATTGTTATGCTTATGGGCGCCTTTGTTACGAT
TCTCAACCAAACGTTGATGAATGTCGCGCTACCAAGTATTATGAAAGATTTTGGAATTACAGCCAGTCAAGGGCAATGGT
TATCGACTGGATTTATGTTGGTTAATGGTGTCATGATTCCAATGACGGCCTTTTTAATTGAACGATTTACAACGCGTCAG
CTTTACTTATTTGCGATGATTACCTTTGCGATTGGTACTGCAATTGGCGGATTTGCGACAGATTATACGATGCTTATTGC
TGGCCGTATGGTACAAGCAATTGGGGCTGGGATTGTTATGCCACTTCTTACTGTTGTGGTCTTAAACTTATTCCCGATGG
AACGAAGAGGGCGAGCAATGGGCTTAATTGGTCTTGCTATGAACTTTGCTCCAGCAATTGGTCCGACACTTTCAGGGTGG
ATTGTTCAACAATATGATTGGCGCAATTTATTCTTTATTATTATTCCTTTCGCGATTCTTGATATTGTCGTAGCGATTTT
CTTACTTAAAAATGTCGGAAAAAGAACTTTCCCGAAACTCGATATCCTTGGGGTTATCATGTCAACAGTCGGGTTTGGTA
GTTTATTATTAGGATTTAGTAATGCTGGAGACCATGATTGGTTAACATGGAAAGTAGCTGGATTTATTATTCTTGGTCTA
GTAGTGCTAGGAATATTTATTCGTTACCAAACAAGCAACAAAGCGCCACTACTTAACTTTAGAGTATTTAAATATCCTAC
CTTTGCGCTTACAACAGCCATTAGTTTCTTTGTTGTAATGGGCCTTTTTGGTGGCATGTTATTACTACCAATTTTCTTAC
AAACTGTTAGAGGATTTTCACCACTAGAATCGGGGCTTGTTCTCTTGCCGGGTGCGCTTGTCACGGCGATACTTTCACCT
GTAACTGGGGTGATGTTTGACCGTTTTGGGGCTAAATATTTATCACTTGTTGGCTTAATTATCATGGCGGGATCGACCTT
CATGTTTACCAACTTGGATGAATCTACTACATTGACTTTTATTATTATTGTACAAACGATTCGTTCGGCTGGGATGGCGA
TGGTTATGATGCCACTGCAAACAGCAGCGCTCAACTCGCTTCCATTAAAACTAGCATCTCATGGTTCAGCAATGTTTAAT
ACCATGCGTCAAGTTGCTGGTTCAATCGGAACAGCGGCGCTTATAACGGTTATGTCTAAAAGTGCTGCGAGTTTCGCTAC
TAAGCTCGGACCAGCCGATGTGATTGGCAAAACTAAAACTGATATTGCCAACCATGTTCTTATCCACGGAATTGAAACGG
CCTTTTTAGTTGCCGGAATTCTTTCTGTAGTTGCTTGTGTCCTAGCACTATTTATACAAAAAAATAGAAGTGCCATGGAT
CCAATTGTTACTAAAACCGAAAAAGCATAA

Upstream 100 bases:

>100_bases
GTTTAGAACCTATCTTAGAGATTGGCGAAGAAAGGTTAAAAGAAATGCTAGAAATGCACCAAGAGATTATTACCATTTTA
GAAAGGGAAGGTGCACGAGA

Downstream 100 bases:

>100_bases
AATAATGAAAAAAGTGTGTTCCATTTTGATGGAATGCACTTTTTTTGTGTAAATAAAGTAAATTTGTAAAAAAATCTAGA
TAACGAACATATGTTGACTT

Product: multidrug-efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MNMKAASTSVKRNGILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTAFLIERFTTRQ
LYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGW
IVQQYDWRNLFFIIIPFAILDIVVAIFLLKNVGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGL
VVLGIFIRYQTSNKAPLLNFRVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLLPGALVTAILSP
VTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTIRSAGMAMVMMPLQTAALNSLPLKLASHGSAMFN
TMRQVAGSIGTAALITVMSKSAASFATKLGPADVIGKTKTDIANHVLIHGIETAFLVAGILSVVACVLALFIQKNRSAMD
PIVTKTEKA

Sequences:

>Translated_489_residues
MNMKAASTSVKRNGILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTAFLIERFTTRQ
LYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGW
IVQQYDWRNLFFIIIPFAILDIVVAIFLLKNVGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGL
VVLGIFIRYQTSNKAPLLNFRVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLLPGALVTAILSP
VTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTIRSAGMAMVMMPLQTAALNSLPLKLASHGSAMFN
TMRQVAGSIGTAALITVMSKSAASFATKLGPADVIGKTKTDIANHVLIHGIETAFLVAGILSVVACVLALFIQKNRSAMD
PIVTKTEKA
>Mature_489_residues
MNMKAASTSVKRNGILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTAFLIERFTTRQ
LYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGW
IVQQYDWRNLFFIIIPFAILDIVVAIFLLKNVGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGL
VVLGIFIRYQTSNKAPLLNFRVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLLPGALVTAILSP
VTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTIRSAGMAMVMMPLQTAALNSLPLKLASHGSAMFN
TMRQVAGSIGTAALITVMSKSAASFATKLGPADVIGKTKTDIANHVLIHGIETAFLVAGILSVVACVLALFIQKNRSAMD
PIVTKTEKA

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=421, Percent_Identity=28.7410926365796, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=28.780487804878, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788710, Length=429, Percent_Identity=26.8065268065268, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1788392, Length=399, Percent_Identity=26.8170426065163, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790195, Length=417, Percent_Identity=24.4604316546763, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323735, Length=323, Percent_Identity=25.3869969040248, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=242, Percent_Identity=26.4462809917355, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=485, Percent_Identity=23.2989690721649, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=326, Percent_Identity=22.0858895705521, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=352, Percent_Identity=21.3068181818182, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52634; Mature: 52634

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHCCCCC
>Mature Secondary Structure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CHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]