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The map label for this gene is pheT

Identifier: 226224208

GI number: 226224208

Start: 1636517

End: 1637134

Strand: Reverse

Name: pheT

Synonym: Lm4b_01618

Alternate gene names: 226224208

Gene position: 1637134-1636517 (Counterclockwise)

Preceding gene: 226224209

Following gene: 226224207

Centisome position: 56.21

GC content: 40.61

Gene sequence:

>618_bases
GTGATAGTAAACGCATTTTATAACAAAGAAGGGGTAGGGGACACTCTACTAATTGAAACAGGCGAAGTAACGTTTGAAAA
TCGTGAATGGGAACGCCGCGGTGATGTAGCGCGAATTTTTGATCGTGAAACAAAAGAGTTGGCGGGATATAACATCTTTA
ATAGCTCCAACTATTTTAATATTACTGCAAATGGTAAGGTGGATTTGACGGAGGAATTAGTAGCGCAAATGAATCAAGTA
ATTGAAAAAGCTGGATTCGAACCGGAAATCGTGGCTGATTTATCTCCTAAATTCGTCGTCGGTTATGTGGAATCGAAAGA
AAAACATCCTAATGCAGATAAATTAAATGTATGTAAAATCAATGTAGGGGATGAAGTATTGCAAATTGTATGCGGTGCCC
CGAACATTGACGCTGGTCAAAAAGTAGTTGTTGCAAAAATCGGTGCGGTTATGCCAAGTGGTTTAATTATCAAACCATCT
AATTTGCGAGGAGAAGATTCTTTCGGCATGGTATGTGCTGCGCGTGAACTTGCGATTCCTGATGCGCCTACGGAAAAGGG
GATTATGGTTCTGGAGGAATCGGCTGTGGTTGGAGAAGCTTTTCCTGTTAATTTCTAA

Upstream 100 bases:

>100_bases
ATGGGCTTGTACCAATTACCTCTTTGCCATTTGTCTATAATAATGGTAAACTTGAACCGATATTTATAATGGCAAAAAAC
AGATTAAAGGAGTAGAAAAC

Downstream 100 bases:

>100_bases
ATAAATGTTTAATTTTGAGCGTCTGATGCTATATCGGGCGCTTTTTTAGTTGCTATTTATTAGATATTGTGAAAACGCCT
TAAATGTAGAACAATAAAAT

Product: phenylalanyl-tRNA synthetase (beta subunit)

Products: AMP; diphosphate; L-phenylalanyl-tRNAPhe [C]

Alternate protein names: Partial phenylalanyl-tRNA synthetase; Partial PheRS; pFRS [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MIVNAFYNKEGVGDTLLIETGEVTFENREWERRGDVARIFDRETKELAGYNIFNSSNYFNITANGKVDLTEELVAQMNQV
IEKAGFEPEIVADLSPKFVVGYVESKEKHPNADKLNVCKINVGDEVLQIVCGAPNIDAGQKVVVAKIGAVMPSGLIIKPS
NLRGEDSFGMVCAARELAIPDAPTEKGIMVLEESAVVGEAFPVNF

Sequences:

>Translated_205_residues
MIVNAFYNKEGVGDTLLIETGEVTFENREWERRGDVARIFDRETKELAGYNIFNSSNYFNITANGKVDLTEELVAQMNQV
IEKAGFEPEIVADLSPKFVVGYVESKEKHPNADKLNVCKINVGDEVLQIVCGAPNIDAGQKVVVAKIGAVMPSGLIIKPS
NLRGEDSFGMVCAARELAIPDAPTEKGIMVLEESAVVGEAFPVNF
>Mature_205_residues
MIVNAFYNKEGVGDTLLIETGEVTFENREWERRGDVARIFDRETKELAGYNIFNSSNYFNITANGKVDLTEELVAQMNQV
IEKAGFEPEIVADLSPKFVVGYVESKEKHPNADKLNVCKINVGDEVLQIVCGAPNIDAGQKVVVAKIGAVMPSGLIIKPS
NLRGEDSFGMVCAARELAIPDAPTEKGIMVLEESAVVGEAFPVNF

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=92, Percent_Identity=53.2608695652174, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR002547 [H]

Pfam domain/function: PF01588 tRNA_bind [H]

EC number: 6.1.1.20 [C]

Molecular weight: Translated: 22369; Mature: 22369

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVNAFYNKEGVGDTLLIETGEVTFENREWERRGDVARIFDRETKELAGYNIFNSSNYFN
CEEEEEECCCCCCCEEEEECCCEEECCCCHHHHCCCHHHHHHHHHHHCCEEEECCCCEEE
ITANGKVDLTEELVAQMNQVIEKAGFEPEIVADLSPKFVVGYVESKEKHPNADKLNVCKI
EEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCCCCCEEEEEE
NVGDEVLQIVCGAPNIDAGQKVVVAKIGAVMPSGLIIKPSNLRGEDSFGMVCAARELAIP
ECCHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCEEEECCCCCCCCCCCEEEEEHHHCCC
DAPTEKGIMVLEESAVVGEAFPVNF
CCCCCCCEEEEECCCEECCEECCCC
>Mature Secondary Structure
MIVNAFYNKEGVGDTLLIETGEVTFENREWERRGDVARIFDRETKELAGYNIFNSSNYFN
CEEEEEECCCCCCCEEEEECCCEEECCCCHHHHCCCHHHHHHHHHHHCCEEEECCCCEEE
ITANGKVDLTEELVAQMNQVIEKAGFEPEIVADLSPKFVVGYVESKEKHPNADKLNVCKI
EEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCCCCCEEEEEE
NVGDEVLQIVCGAPNIDAGQKVVVAKIGAVMPSGLIIKPSNLRGEDSFGMVCAARELAIP
ECCHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCEEEECCCCCCCCCCCEEEEEHHHCCC
DAPTEKGIMVLEESAVVGEAFPVNF
CCCCCCCEEEEECCCEECCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.009 {ATP}} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} [C]

Substrates: ATP; @PHEN01.txt*phenylalanine!; tRNAPhe [C]

Specific reaction: ATP + @PHEN01.txt*phenylalanine! + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]