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The map label for this gene is tyrS

Identifier: 226224199

GI number: 226224199

Start: 1624990

End: 1626249

Strand: Reverse

Name: tyrS

Synonym: Lm4b_01609

Alternate gene names: 226224199

Gene position: 1626249-1624990 (Counterclockwise)

Preceding gene: 226224200

Following gene: 226224198

Centisome position: 55.83

GC content: 38.81

Gene sequence:

>1260_bases
ATGAATATTATTGATGAATTAGAGTGGCGCGGAGCTATTTACCAGCAAACAGATGAAGAAGGATTACGCAAATGGGTAGA
AGAAAAACAGATTTCTTTGTACTGCGGGATTGATCCATCTGGAGACTCGATGCATATTGGTCATTTAATTCCATTCATGA
TTTTACGCCGTTTCCAAAACGCTGGGCACCGTCCGATTATTTTAGTTGGTGGAGCGACAGGAACTATTGGCGATCCGAGT
GGTAAAAAGGAAGAGCGTAAGTTACAATCAATGGAACAAATTAGTAAAAATGTGGAAAGTTTGCGCGTGCAACTTGGAAA
GATTTTTGATTTTGAAGGTAATTCTGCGGCGAGCATGGTGAACAACTATGATTGGACAAAAGATGTGAGTATCCTTGATT
TCTTGCGTGACTATGGAAAAGAATTCAACGTGAACACGATGCTTTCAAAAGATATCGTTGCTAGTCGTTTAGAAGTAGGG
ATTTCATTCACGGAGTTCGCTTACCAAATTTTACAAGCGATGGACTTTAATCACTTATATGAATTTAATGATTGCCGTTT
GCAAATTGGTGGTAGTGACCAGTGGGGGAATATTACTGCTGGACTTGATTTGATTCGTAAAAAACAAGGCGAAAATGCCA
AAGCTTTCGGTTTAACAATTCCTCTTTTAACAAAAGCTGATGGAACGAAATTTGGGAAATCAGAAGGCGGGGCAATTTGG
TTAAATCCAGAAAAAACAACGCCATATGAGTTTTATCAATTCTGGATTAACACAGATGATCGTGATGTTGTGAAATACCT
GAAGTACTTTACATTCCTAACAGAAGCAGAAATTGATGAGTTAGCAAAACAAGTAGAAACAGAACCGCATTTACGAGCTG
CACAAAAAACGTTAGCGGCAGAAATGACCAAATTTGTGCATAGTGAAGAAGCGTTGGAACAAGCACTTAAAATTTCAAAA
GCACTATTTAGTGGTGATGTAAAAGCGCTGACTGCGGATGAAATTGAACAAGGCTTCAAAGACGTTCCGACATTTGTTGC
AGAAGATACGGAAGCAAACTTGGTAGATTGGTTAGTAACACTTGGTATCGAACCTTCCAAACGTCAAGCGCGTGAAGATG
TAGGCAACGGGGCTATTTACATTAACGGCGAACGTCAGCAAGATTTGGAAAAAATCATGGATGCAAGTGACCGGATTGAA
AATAAATTTACGATTGTAAGACGCGGGAAGAAAAAATACTTCTTAGTTTCTTATAAATAA

Upstream 100 bases:

>100_bases
TAAAAGTAGGGTGGTACCACGTTAATTAATACGTCCCTTCGCTAAACTGTTTAGCGGAGGGGCGTTTTTTATGTCCAAAA
ATAAAAGGAGAATGATGAAA

Downstream 100 bases:

>100_bases
GAAGAAAAGCACTCGTTTTTAAAGGCGAGTGCTTTTTTTGTGCATAAAAAAACCTTACAACCACAACCGAAGTCATGAAT
TGTAAGGTTTTTAGTTCTAA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNIIDELEWRGAIYQQTDEEGLRKWVEEKQISLYCGIDPSGDSMHIGHLIPFMILRRFQNAGHRPIILVGGATGTIGDPS
GKKEERKLQSMEQISKNVESLRVQLGKIFDFEGNSAASMVNNYDWTKDVSILDFLRDYGKEFNVNTMLSKDIVASRLEVG
ISFTEFAYQILQAMDFNHLYEFNDCRLQIGGSDQWGNITAGLDLIRKKQGENAKAFGLTIPLLTKADGTKFGKSEGGAIW
LNPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLTEAEIDELAKQVETEPHLRAAQKTLAAEMTKFVHSEEALEQALKISK
ALFSGDVKALTADEIEQGFKDVPTFVAEDTEANLVDWLVTLGIEPSKRQAREDVGNGAIYINGERQQDLEKIMDASDRIE
NKFTIVRRGKKKYFLVSYK

Sequences:

>Translated_419_residues
MNIIDELEWRGAIYQQTDEEGLRKWVEEKQISLYCGIDPSGDSMHIGHLIPFMILRRFQNAGHRPIILVGGATGTIGDPS
GKKEERKLQSMEQISKNVESLRVQLGKIFDFEGNSAASMVNNYDWTKDVSILDFLRDYGKEFNVNTMLSKDIVASRLEVG
ISFTEFAYQILQAMDFNHLYEFNDCRLQIGGSDQWGNITAGLDLIRKKQGENAKAFGLTIPLLTKADGTKFGKSEGGAIW
LNPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLTEAEIDELAKQVETEPHLRAAQKTLAAEMTKFVHSEEALEQALKISK
ALFSGDVKALTADEIEQGFKDVPTFVAEDTEANLVDWLVTLGIEPSKRQAREDVGNGAIYINGERQQDLEKIMDASDRIE
NKFTIVRRGKKKYFLVSYK
>Mature_419_residues
MNIIDELEWRGAIYQQTDEEGLRKWVEEKQISLYCGIDPSGDSMHIGHLIPFMILRRFQNAGHRPIILVGGATGTIGDPS
GKKEERKLQSMEQISKNVESLRVQLGKIFDFEGNSAASMVNNYDWTKDVSILDFLRDYGKEFNVNTMLSKDIVASRLEVG
ISFTEFAYQILQAMDFNHLYEFNDCRLQIGGSDQWGNITAGLDLIRKKQGENAKAFGLTIPLLTKADGTKFGKSEGGAIW
LNPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLTEAEIDELAKQVETEPHLRAAQKTLAAEMTKFVHSEEALEQALKISK
ALFSGDVKALTADEIEQGFKDVPTFVAEDTEANLVDWLVTLGIEPSKRQAREDVGNGAIYINGERQQDLEKIMDASDRIE
NKFTIVRRGKKKYFLVSYK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=403, Percent_Identity=37.2208436724566, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1787925, Length=422, Percent_Identity=48.8151658767773, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25145802, Length=398, Percent_Identity=36.9346733668342, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI25145805, Length=327, Percent_Identity=35.1681957186544, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=38.0410022779043, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI20130383, Length=437, Percent_Identity=37.5286041189931, Blast_Score=296, Evalue=1e-80,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47608; Mature: 47608

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIDELEWRGAIYQQTDEEGLRKWVEEKQISLYCGIDPSGDSMHIGHLIPFMILRRFQN
CCCHHHHHHCCHHHCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHC
AGHRPIILVGGATGTIGDPSGKKEERKLQSMEQISKNVESLRVQLGKIFDFEGNSAASMV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHH
NNYDWTKDVSILDFLRDYGKEFNVNTMLSKDIVASRLEVGISFTEFAYQILQAMDFNHLY
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHCE
EFNDCRLQIGGSDQWGNITAGLDLIRKKQGENAKAFGLTIPLLTKADGTKFGKSEGGAIW
ECCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEE
LNPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLTEAEIDELAKQVETEPHLRAAQKTLAA
ECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EMTKFVHSEEALEQALKISKALFSGDVKALTADEIEQGFKDVPTFVAEDTEANLVDWLVT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCHHHHCCCCHHHHHHHHH
LGIEPSKRQAREDVGNGAIYINGERQQDLEKIMDASDRIENKFTIVRRGKKKYFLVSYK
HCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHCHHHHHCCCEEEEECCCCEEEEEEEC
>Mature Secondary Structure
MNIIDELEWRGAIYQQTDEEGLRKWVEEKQISLYCGIDPSGDSMHIGHLIPFMILRRFQN
CCCHHHHHHCCHHHCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHC
AGHRPIILVGGATGTIGDPSGKKEERKLQSMEQISKNVESLRVQLGKIFDFEGNSAASMV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHH
NNYDWTKDVSILDFLRDYGKEFNVNTMLSKDIVASRLEVGISFTEFAYQILQAMDFNHLY
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHCE
EFNDCRLQIGGSDQWGNITAGLDLIRKKQGENAKAFGLTIPLLTKADGTKFGKSEGGAIW
ECCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEE
LNPEKTTPYEFYQFWINTDDRDVVKYLKYFTFLTEAEIDELAKQVETEPHLRAAQKTLAA
ECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EMTKFVHSEEALEQALKISKALFSGDVKALTADEIEQGFKDVPTFVAEDTEANLVDWLVT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCHHHHCCCCHHHHHHHHH
LGIEPSKRQAREDVGNGAIYINGERQQDLEKIMDASDRIENKFTIVRRGKKKYFLVSYK
HCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHCHHHHHCCCEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]