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The map label for this gene is ppnK

Identifier: 226224187

GI number: 226224187

Start: 1611509

End: 1612312

Strand: Direct

Name: ppnK

Synonym: Lm4b_01597

Alternate gene names: 226224187

Gene position: 1611509-1612312 (Clockwise)

Preceding gene: 226224181

Following gene: 226224196

Centisome position: 55.33

GC content: 35.45

Gene sequence:

>804_bases
ATGGCAAAAACTATTTTTTATTTTTCCTATCGAAAAACAGAAGAACTACATGCAAAAGCAAAAGAATTGAAGAAAATTAC
GACGGATTATGGATATGAATTAACGGATGACTATCAAAAAGCAAACGTTATTATCAGCATTGGTGGTGATGGTGCTTTTC
TTAAATCTGTAAGAGAAACTGGCTTCCGTCAAGATTGTTTATATGCAGGGATTGCGCTAACAGAGCAATTAGGTCAATAC
TGTGACTTCCATATTAATCAATTGGATGAAATCATCAAGGCAGCCATTGAAGATCGTTGGTTAGTAAGACGTTATCCAAC
TATCTATGGAACAGTGAATAACACCAAAGCTTTTTATGTATTAAATGAATTTAACATTCGTTCTTCTATTATTAGAACGC
TTACGATGGATCTTTATATTAATGATTCTCACTTCGAAACTTTCCGCGGAGATGGCATGGTTATTTCCACACCAACAGGA
AGTACCGCTTATAATAAATCAGTGAACGGTTCTATCGTGGACCCACTTCTTCCATCTATGCAAGTAAGTGAACTGGCTTC
TATTAACAATAACAAATTCCGCACACTTGGTTCTTCGTTCATTCTTAGTCCAAAACGCAAACTACGTATTGAAATTGCGT
CTGAAGAAGGAAATAACGAATTCCCGATGATTGGAATGGACAGCGAAGCACTTAGTATCCAACACGTGCATGAAGTTAAT
TTAGAAGTAGGCGATCGTTTTATCAATATTATCAAACTGCCTAAAAATTCTTTCTGGGATAAAGTGAAACGTAATTTCTT
ATAA

Upstream 100 bases:

>100_bases
TGTCCCATTTCACTTTTTTACTTATGATGAAAGAAATTGCTCTGAAGTCATGTTATAATAAGTTTGTTGATTAAAATTGG
ATTCTGGAGGGACAACTAAC

Downstream 100 bases:

>100_bases
TAAAAAAGTCTGGCACTTACGCCAGACTTTTTTATTTTGCTTCCAAGATGGCTGCTAGTAATGCTTTTTGTGCATGAAGC
CGATTTCCCGCTTGTTGATA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 2

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MAKTIFYFSYRKTEELHAKAKELKKITTDYGYELTDDYQKANVIISIGGDGAFLKSVRETGFRQDCLYAGIALTEQLGQY
CDFHINQLDEIIKAAIEDRWLVRRYPTIYGTVNNTKAFYVLNEFNIRSSIIRTLTMDLYINDSHFETFRGDGMVISTPTG
STAYNKSVNGSIVDPLLPSMQVSELASINNNKFRTLGSSFILSPKRKLRIEIASEEGNNEFPMIGMDSEALSIQHVHEVN
LEVGDRFINIIKLPKNSFWDKVKRNFL

Sequences:

>Translated_267_residues
MAKTIFYFSYRKTEELHAKAKELKKITTDYGYELTDDYQKANVIISIGGDGAFLKSVRETGFRQDCLYAGIALTEQLGQY
CDFHINQLDEIIKAAIEDRWLVRRYPTIYGTVNNTKAFYVLNEFNIRSSIIRTLTMDLYINDSHFETFRGDGMVISTPTG
STAYNKSVNGSIVDPLLPSMQVSELASINNNKFRTLGSSFILSPKRKLRIEIASEEGNNEFPMIGMDSEALSIQHVHEVN
LEVGDRFINIIKLPKNSFWDKVKRNFL
>Mature_266_residues
AKTIFYFSYRKTEELHAKAKELKKITTDYGYELTDDYQKANVIISIGGDGAFLKSVRETGFRQDCLYAGIALTEQLGQYC
DFHINQLDEIIKAAIEDRWLVRRYPTIYGTVNNTKAFYVLNEFNIRSSIIRTLTMDLYINDSHFETFRGDGMVISTPTGS
TAYNKSVNGSIVDPLLPSMQVSELASINNNKFRTLGSSFILSPKRKLRIEIASEEGNNEFPMIGMDSEALSIQHVHEVNL
EVGDRFINIIKLPKNSFWDKVKRNFL

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6325068, Length=216, Percent_Identity=26.3888888888889, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK2_LISIN (P65771)

Other databases:

- EMBL:   AL596169
- PIR:   AC1636
- RefSeq:   NP_470964.1
- ProteinModelPortal:   P65771
- SMR:   P65771
- GeneID:   1130251
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin1628
- GenoList:   LIN1628
- HOGENOM:   HBG314020
- OMA:   RGDGMII
- ProtClustDB:   PRK03501
- BioCyc:   LINN272626:LIN1628-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30499; Mature: 30368

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIFYFSYRKTEELHAKAKELKKITTDYGYELTDDYQKANVIISIGGDGAFLKSVRET
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCCHHHHHHHHHC
GFRQDCLYAGIALTEQLGQYCDFHINQLDEIIKAAIEDRWLVRRYPTIYGTVNNTKAFYV
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHEEECCEEEEEECCCEEEEE
LNEFNIRSSIIRTLTMDLYINDSHFETFRGDGMVISTPTGSTAYNKSVNGSIVDPLLPSM
EEECCHHHHHHHHEEEEEEEECCCCCEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCH
QVSELASINNNKFRTLGSSFILSPKRKLRIEIASEEGNNEFPMIGMDSEALSIQHVHEVN
HHHHHHHCCCCCEEECCCCEEECCCEEEEEEEECCCCCCCCCEEECCCCHHEEEHHEECC
LEVGDRFINIIKLPKNSFWDKVKRNFL
HHHCCEEEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure 
AKTIFYFSYRKTEELHAKAKELKKITTDYGYELTDDYQKANVIISIGGDGAFLKSVRET
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCCHHHHHHHHHC
GFRQDCLYAGIALTEQLGQYCDFHINQLDEIIKAAIEDRWLVRRYPTIYGTVNNTKAFYV
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHEEECCEEEEEECCCEEEEE
LNEFNIRSSIIRTLTMDLYINDSHFETFRGDGMVISTPTGSTAYNKSVNGSIVDPLLPSM
EEECCHHHHHHHHEEEEEEEECCCCCEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCH
QVSELASINNNKFRTLGSSFILSPKRKLRIEIASEEGNNEFPMIGMDSEALSIQHVHEVN
HHHHHHHCCCCCEEECCCCEEECCCEEEEEEEECCCCCCCCCEEECCCCHHEEEHHEECC
LEVGDRFINIIKLPKNSFWDKVKRNFL
HHHCCEEEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669