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The map label for this gene is minD

Identifier: 226224145

GI number: 226224145

Start: 1562323

End: 1563123

Strand: Reverse

Name: minD

Synonym: Lm4b_01555

Alternate gene names: 226224145

Gene position: 1563123-1562323 (Counterclockwise)

Preceding gene: 226224146

Following gene: 226224144

Centisome position: 53.67

GC content: 36.58

Gene sequence:

>801_bases
ATGGGAGAAGCTATAGTCATTACTTCTGGGAAAGGTGGAGTAGGAAAAACTACTTCAACTGCTAACTTGGGAACGGCACT
TGCTCTTCAAGGTAAGAAAGTGTGCTTGATTGATATGGATATCGGCCTTCGCAATTTAGATGTTGTTTTAGGTCTTGAAA
ATCGAATTATTTATGATTTAGTAGATGTGGTAGAAGGTCGCTGTAAAATTCATCAAGCAATGATTAAAGATAAACGTTTT
GATGATTTACTTTTCTTGCTTCCAGCGGCGCAAACTACTGATAAAAATGCTGTTTCGGGTGAACAAATGGTGGATTTAAT
TAATCAACTCCGCCCAGACTATGATTTTATTTTAATTGATTGTCCTGCAGGAATTGAAACTGGCTATAAAAATGCCGTTG
CTGGTGCAGACAAAGCAATTGTAGTTACAACGCCAGAAATATCAGCAGTTCGTGATGCAGATAGAATTATCGGTTTACTG
GAAAAAGAAGATATTGAGCCACCAAAATTAATTATTAACCGTATTCGTACACAAATGATGATGAATGGTGATGTAATGGA
TATTGATGAAATTACCACACATTTATCAATTGAATTACTTGGTATTATCATTGATGATGATGAAGTTATTCGCTCTTCGA
ACAGTGGGGATCCTGTTGCTATGTTACCAAATAACCGTGCTTCACAAGGTTATCGTAATATTGCTCGACGTATTCTTGGA
GAATCTATCCCTTTAATGTCGATTGAGACAAAAAAAGCGGGATTTTTCGCTCGCTTAAAGCAACTTTTTAGCGGGAAATA
A

Upstream 100 bases:

>100_bases
CTGCATTTGTAAATGATGCAGGTGAGATAGTAATTGATGGAATACATAAGATAAGAAAAATTAGACCTGAAATTTCTAAT
TTTCAAGGAGGGCGTTAAAC

Downstream 100 bases:

>100_bases
AAAATTAGACCCTTTATCTTTAAGATGAAGGGTCTTTATAAAAGGTGATTGTATGAAAAAGCTAGTAGTAAGTGCGGCTC
CTATAGAAAAACGAATAGCA

Product: cell division inhibitor (septum placement) protein MinD

Products: NA

Alternate protein names: Cell division inhibitor minD [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MGEAIVITSGKGGVGKTTSTANLGTALALQGKKVCLIDMDIGLRNLDVVLGLENRIIYDLVDVVEGRCKIHQAMIKDKRF
DDLLFLLPAAQTTDKNAVSGEQMVDLINQLRPDYDFILIDCPAGIETGYKNAVAGADKAIVVTTPEISAVRDADRIIGLL
EKEDIEPPKLIINRIRTQMMMNGDVMDIDEITTHLSIELLGIIIDDDEVIRSSNSGDPVAMLPNNRASQGYRNIARRILG
ESIPLMSIETKKAGFFARLKQLFSGK

Sequences:

>Translated_266_residues
MGEAIVITSGKGGVGKTTSTANLGTALALQGKKVCLIDMDIGLRNLDVVLGLENRIIYDLVDVVEGRCKIHQAMIKDKRF
DDLLFLLPAAQTTDKNAVSGEQMVDLINQLRPDYDFILIDCPAGIETGYKNAVAGADKAIVVTTPEISAVRDADRIIGLL
EKEDIEPPKLIINRIRTQMMMNGDVMDIDEITTHLSIELLGIIIDDDEVIRSSNSGDPVAMLPNNRASQGYRNIARRILG
ESIPLMSIETKKAGFFARLKQLFSGK
>Mature_265_residues
GEAIVITSGKGGVGKTTSTANLGTALALQGKKVCLIDMDIGLRNLDVVLGLENRIIYDLVDVVEGRCKIHQAMIKDKRFD
DLLFLLPAAQTTDKNAVSGEQMVDLINQLRPDYDFILIDCPAGIETGYKNAVAGADKAIVVTTPEISAVRDADRIIGLLE
KEDIEPPKLIINRIRTQMMMNGDVMDIDEITTHLSIELLGIIIDDDEVIRSSNSGDPVAMLPNNRASQGYRNIARRILGE
SIPLMSIETKKAGFFARLKQLFSGK

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787423, Length=274, Percent_Identity=45.2554744525547, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR010223 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 29067; Mature: 28936

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEAIVITSGKGGVGKTTSTANLGTALALQGKKVCLIDMDIGLRNLDVVLGLENRIIYDL
CCCEEEEECCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEEEEEECCCHHHHHH
VDVVEGRCKIHQAMIKDKRFDDLLFLLPAAQTTDKNAVSGEQMVDLINQLRPDYDFILID
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
CPAGIETGYKNAVAGADKAIVVTTPEISAVRDADRIIGLLEKEDIEPPKLIINRIRTQMM
CCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MNGDVMDIDEITTHLSIELLGIIIDDDEVIRSSNSGDPVAMLPNNRASQGYRNIARRILG
HCCCCCCHHHHHHHEEEEEEEEEECCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHC
ESIPLMSIETKKAGFFARLKQLFSGK
CCCCEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GEAIVITSGKGGVGKTTSTANLGTALALQGKKVCLIDMDIGLRNLDVVLGLENRIIYDL
CCEEEEECCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCEEEEEEECCCHHHHHH
VDVVEGRCKIHQAMIKDKRFDDLLFLLPAAQTTDKNAVSGEQMVDLINQLRPDYDFILID
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
CPAGIETGYKNAVAGADKAIVVTTPEISAVRDADRIIGLLEKEDIEPPKLIINRIRTQMM
CCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MNGDVMDIDEITTHLSIELLGIIIDDDEVIRSSNSGDPVAMLPNNRASQGYRNIARRILG
HCCCCCCHHHHHHHEEEEEEEEEECCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHC
ESIPLMSIETKKAGFFARLKQLFSGK
CCCCEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1400225; 8459776; 1400224; 9384377 [H]