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The map label for this gene is rne [H]

Identifier: 226224144

GI number: 226224144

Start: 1560909

End: 1562270

Strand: Reverse

Name: rne [H]

Synonym: Lm4b_01554

Alternate gene names: 226224144

Gene position: 1562270-1560909 (Counterclockwise)

Preceding gene: 226224145

Following gene: 226224143

Centisome position: 53.64

GC content: 36.71

Gene sequence:

>1362_bases
ATGAAAAAGCTAGTAGTAAGTGCGGCTCCTATAGAAAAACGAATAGCAGCAATGGAAGATGATTTGTTAATAGATATTGA
AATTGTACGACCTTCTGAAAAAGTTCAAGTTGGTGATATTTATTATGGATTTATTCAAAAAATCGATCGAAAAGTGGAAG
CAGCTTTTGTTGATTTAGGTAATAATAACAAAGGTTTCATCCATTTAAAAGATATTCCGGAAAGCTATGATAAAACGCAA
GGTGCTAAAATTCCAGTTCAAATTGTGCGGGAAGGCAGCGCGACGAAACTTCCGTTATTAACTGGTATACTGGAATTTTC
TGGTGACTTGCTTATTTATTTGTATGGAAAAGAATATATTTCTATTTCTAAAAGAATAGGAGACAAAATCAGATTAAAGA
AAATAACGGAAACCATGGTGGAAGAAAATGAAGCGGTAATTATTCGATCTAATGCAGAATTTGCGACGAAAGAGCAATTG
CAACAAGCCTTGGTTCAAGCGAGAGATAAACATCAAACATTATTAAAAGAAGCTAGCAAGCGGAAAAAACCTGGCTTACT
ATTATCAGTGGCTTCGGGAATTTTAAATAGTACGAAACAGTTCATTCAACGATACGCACCATCAGAAGTAATTACGGATG
ATTTTGAGTTTGCTAAAATGCTAGAAATGCTATGGCCGGATAAGCAAATCATCATAAAGAAAAGCGCTGATTTATTTGCG
GACCTAGGTATCAAAGCTCAAATAGATAGGCTCGCACGACCAGTGGTTCATTTAAGTAATGGTTCTTCTTTATTTATTGA
AAAAACAGAAGCGATGTGGGTTGTTGATGTAAACTCAGGTAAATTTAAAGGTACCACAGCAAAAGAAAAAACAGTAGAAT
CAGTAAATCTCAAAGCAGTTCCTGAAATCCTTCGACAAATTAGGTTACGTAATATGAGTGGGATGATATTAGTGGATTTT
ATTGGAGGAATGTCAGAATTGGGCTACACAGCGCTTTATGAAGCGTTAGAGATCAAAACTCGCGAAGAATATATTACAAC
ACAAGTTGCTGCTTTATCGCAGTCAGGGCTATTACAACTGACGAGACGCAAAAAACAGCAATCCTTTTTAGAAACGACAA
CGATGCCTTGTCCAGTTTGTTATGCGACGGGCCATGTGGCTTCCAAAGAGACGCTTGCTTTCGAATTAGAACGAGAGCTT
TCAGGGATTATGCAAAATGAGCCAAGCAATATCCAAATTATAACGACAGAAGATGTTTTAGATACTTTTTTAGATTTGAA
TACGCTAAGTAATGCGCCAATTGATTGGGAAGTGGCGGATGAAAGAGTCCCGTTTTATCAAATTGGCCGGGTGGAAAACT
AA

Upstream 100 bases:

>100_bases
AAAGCGGGATTTTTCGCTCGCTTAAAGCAACTTTTTAGCGGGAAATAAAAAATTAGACCCTTTATCTTTAAGATGAAGGG
TCTTTATAAAAGGTGATTGT

Downstream 100 bases:

>100_bases
AATCAACAATTGGTTGACATAGGCTGCTTGCTTATGCTATCATTTTACTGTTATGTTTGTAGCGCACCAAGCTACAACCG
CACAGAGCTGGGTCAAGTAT

Product: hypothetical ribonuclease G

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKKLVVSAAPIEKRIAAMEDDLLIDIEIVRPSEKVQVGDIYYGFIQKIDRKVEAAFVDLGNNNKGFIHLKDIPESYDKTQ
GAKIPVQIVREGSATKLPLLTGILEFSGDLLIYLYGKEYISISKRIGDKIRLKKITETMVEENEAVIIRSNAEFATKEQL
QQALVQARDKHQTLLKEASKRKKPGLLLSVASGILNSTKQFIQRYAPSEVITDDFEFAKMLEMLWPDKQIIIKKSADLFA
DLGIKAQIDRLARPVVHLSNGSSLFIEKTEAMWVVDVNSGKFKGTTAKEKTVESVNLKAVPEILRQIRLRNMSGMILVDF
IGGMSELGYTALYEALEIKTREEYITTQVAALSQSGLLQLTRRKKQQSFLETTTMPCPVCYATGHVASKETLAFELEREL
SGIMQNEPSNIQIITTEDVLDTFLDLNTLSNAPIDWEVADERVPFYQIGRVEN

Sequences:

>Translated_453_residues
MKKLVVSAAPIEKRIAAMEDDLLIDIEIVRPSEKVQVGDIYYGFIQKIDRKVEAAFVDLGNNNKGFIHLKDIPESYDKTQ
GAKIPVQIVREGSATKLPLLTGILEFSGDLLIYLYGKEYISISKRIGDKIRLKKITETMVEENEAVIIRSNAEFATKEQL
QQALVQARDKHQTLLKEASKRKKPGLLLSVASGILNSTKQFIQRYAPSEVITDDFEFAKMLEMLWPDKQIIIKKSADLFA
DLGIKAQIDRLARPVVHLSNGSSLFIEKTEAMWVVDVNSGKFKGTTAKEKTVESVNLKAVPEILRQIRLRNMSGMILVDF
IGGMSELGYTALYEALEIKTREEYITTQVAALSQSGLLQLTRRKKQQSFLETTTMPCPVCYATGHVASKETLAFELEREL
SGIMQNEPSNIQIITTEDVLDTFLDLNTLSNAPIDWEVADERVPFYQIGRVEN
>Mature_453_residues
MKKLVVSAAPIEKRIAAMEDDLLIDIEIVRPSEKVQVGDIYYGFIQKIDRKVEAAFVDLGNNNKGFIHLKDIPESYDKTQ
GAKIPVQIVREGSATKLPLLTGILEFSGDLLIYLYGKEYISISKRIGDKIRLKKITETMVEENEAVIIRSNAEFATKEQL
QQALVQARDKHQTLLKEASKRKKPGLLLSVASGILNSTKQFIQRYAPSEVITDDFEFAKMLEMLWPDKQIIIKKSADLFA
DLGIKAQIDRLARPVVHLSNGSSLFIEKTEAMWVVDVNSGKFKGTTAKEKTVESVNLKAVPEILRQIRLRNMSGMILVDF
IGGMSELGYTALYEALEIKTREEYITTQVAALSQSGLLQLTRRKKQQSFLETTTMPCPVCYATGHVASKETLAFELEREL
SGIMQNEPSNIQIITTEDVLDTFLDLNTLSNAPIDWEVADERVPFYQIGRVEN

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=427, Percent_Identity=29.03981264637, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87082235, Length=436, Percent_Identity=26.8348623853211, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 50952; Mature: 50952

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVVSAAPIEKRIAAMEDDLLIDIEIVRPSEKVQVGDIYYGFIQKIDRKVEAAFVDLG
CCCEEEECCCHHHHHHHCCCCEEEEEEEECCCCCEEECHHHHHHHHHHHHHHHEEEEECC
NNNKGFIHLKDIPESYDKTQGAKIPVQIVREGSATKLPLLTGILEFSGDLLIYLYGKEYI
CCCCCEEEECCCCCHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCHHH
SISKRIGDKIRLKKITETMVEENEAVIIRSNAEFATKEQLQQALVQARDKHQTLLKEASK
HHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKKPGLLLSVASGILNSTKQFIQRYAPSEVITDDFEFAKMLEMLWPDKQIIIKKSADLFA
CCCCCEEHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHCCCCEEEEEECCHHHH
DLGIKAQIDRLARPVVHLSNGSSLFIEKTEAMWVVDVNSGKFKGTTAKEKTVESVNLKAV
HCCCHHHHHHHHCCEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHCCHHHH
PEILRQIRLRNMSGMILVDFIGGMSELGYTALYEALEIKTREEYITTQVAALSQSGLLQL
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TRRKKQQSFLETTTMPCPVCYATGHVASKETLAFELERELSGIMQNEPSNIQIITTEDVL
HHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHH
DTFLDLNTLSNAPIDWEVADERVPFYQIGRVEN
HHHHHHHHCCCCCCCEEECCCCCCEEECCCCCC
>Mature Secondary Structure
MKKLVVSAAPIEKRIAAMEDDLLIDIEIVRPSEKVQVGDIYYGFIQKIDRKVEAAFVDLG
CCCEEEECCCHHHHHHHCCCCEEEEEEEECCCCCEEECHHHHHHHHHHHHHHHEEEEECC
NNNKGFIHLKDIPESYDKTQGAKIPVQIVREGSATKLPLLTGILEFSGDLLIYLYGKEYI
CCCCCEEEECCCCCHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCHHH
SISKRIGDKIRLKKITETMVEENEAVIIRSNAEFATKEQLQQALVQARDKHQTLLKEASK
HHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKKPGLLLSVASGILNSTKQFIQRYAPSEVITDDFEFAKMLEMLWPDKQIIIKKSADLFA
CCCCCEEHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHCCCCEEEEEECCHHHH
DLGIKAQIDRLARPVVHLSNGSSLFIEKTEAMWVVDVNSGKFKGTTAKEKTVESVNLKAV
HCCCHHHHHHHHCCEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHCCHHHH
PEILRQIRLRNMSGMILVDFIGGMSELGYTALYEALEIKTREEYITTQVAALSQSGLLQL
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TRRKKQQSFLETTTMPCPVCYATGHVASKETLAFELERELSGIMQNEPSNIQIITTEDVL
HHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHH
DTFLDLNTLSNAPIDWEVADERVPFYQIGRVEN
HHHHHHHHCCCCCCCEEECCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]