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The map label for this gene is glpF [H]

Identifier: 226224140

GI number: 226224140

Start: 1558620

End: 1559438

Strand: Reverse

Name: glpF [H]

Synonym: Lm4b_01549

Alternate gene names: 226224140

Gene position: 1559438-1558620 (Counterclockwise)

Preceding gene: 226224141

Following gene: 226224139

Centisome position: 53.54

GC content: 41.03

Gene sequence:

>819_bases
ATGATTGACACAAGTTTAGCAACACAATTTCTAGGTGAAGTTATTGGTACGGCTATCCTAATTATTTTAGGTGCTGGTGT
CGTAGCAGGTGTTTCACTGAAAAGATCTAAAGCGGAGAATGGCGGCTGGGTCGTTATTACTTTAGCTTGGGGGCTAGGTG
TTACAATGGGTGTATATGTTTCAGGATATATGAGTATGGCCCATCTTAACCCTGCTGTAACTATTGGTATGGCGCTTGCA
GGTGCTTTCCCGTGGAATTACGTATTGCCATATATTCTTGCTCAGTTTATCGGAGCATTTATTGGGGCAACGCTAATATG
GTTACATTACTATCCTCACTGGAAACAAACAGAAGACAAACCAACAAAACTAGGTGTTTTTGCAACGGCGCCAGCTATTC
GCCATTTTACATCTAACTTTTTTGGTGAAGCATTAGGTACTTTTATTTTAGTATTTGGATTACTATCACTAGGTGCAAAT
TCTTTTTCAGATGGATTAAATCCACTTGTTGTCGGCGCATTAATCGTAGCTATCGGGATGTCTCTTGGTGGAACTACTGG
TTATGCCATCAACCCAGCTCGTGACCTTGGACCGAGAATTGCGCATTTTGTTTGGCCGATTTCTGGCAAAGGTGGATCTG
ATTGGGGTTACTCATGGGTTCCAGTTATTGGACCAATCATGGGCGGTGCATTAGGTGCTTTAGGGTATAATGCCATTATT
AACGGAGAACTAGGAATGTGGTTCTGGGTGTTCGCAGTATTATTTGTTTTAATTCTTATTTTAACGATGCAACTCGACAA
GAAAAAAGATTTAGCATAA

Upstream 100 bases:

>100_bases
CATTCTTTTTTTGAAGTTAATTGATAATCGGAATGTAAGGGGACTAGTAAGTGTTAAAATTCCGGTGATACAAATAAGCA
AGAAGAAGGAGTTGGAAAAG

Downstream 100 bases:

>100_bases
GCTAGAATAATAACTAGCTCCAAGCGAAGGTCTTCGGGCTTTCGTTGTACTTAAAACTAGAGGAGGAATTTTACATGGAA
AAGAAATATATTTTAGCACT

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MIDTSLATQFLGEVIGTAILIILGAGVVAGVSLKRSKAENGGWVVITLAWGLGVTMGVYVSGYMSMAHLNPAVTIGMALA
GAFPWNYVLPYILAQFIGAFIGATLIWLHYYPHWKQTEDKPTKLGVFATAPAIRHFTSNFFGEALGTFILVFGLLSLGAN
SFSDGLNPLVVGALIVAIGMSLGGTTGYAINPARDLGPRIAHFVWPISGKGGSDWGYSWVPVIGPIMGGALGALGYNAII
NGELGMWFWVFAVLFVLILILTMQLDKKKDLA

Sequences:

>Translated_272_residues
MIDTSLATQFLGEVIGTAILIILGAGVVAGVSLKRSKAENGGWVVITLAWGLGVTMGVYVSGYMSMAHLNPAVTIGMALA
GAFPWNYVLPYILAQFIGAFIGATLIWLHYYPHWKQTEDKPTKLGVFATAPAIRHFTSNFFGEALGTFILVFGLLSLGAN
SFSDGLNPLVVGALIVAIGMSLGGTTGYAINPARDLGPRIAHFVWPISGKGGSDWGYSWVPVIGPIMGGALGALGYNAII
NGELGMWFWVFAVLFVLILILTMQLDKKKDLA
>Mature_272_residues
MIDTSLATQFLGEVIGTAILIILGAGVVAGVSLKRSKAENGGWVVITLAWGLGVTMGVYVSGYMSMAHLNPAVTIGMALA
GAFPWNYVLPYILAQFIGAFIGATLIWLHYYPHWKQTEDKPTKLGVFATAPAIRHFTSNFFGEALGTFILVFGLLSLGAN
SFSDGLNPLVVGALIVAIGMSLGGTTGYAINPARDLGPRIAHFVWPISGKGGSDWGYSWVPVIGPIMGGALGALGYNAII
NGELGMWFWVFAVLFVLILILTMQLDKKKDLA

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=260, Percent_Identity=36.5384615384615, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI157266307, Length=259, Percent_Identity=35.9073359073359, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI22538420, Length=253, Percent_Identity=37.5494071146245, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4502187, Length=253, Percent_Identity=35.9683794466403, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI310133356, Length=211, Percent_Identity=35.0710900473934, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI310114181, Length=211, Percent_Identity=35.0710900473934, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4755125, Length=198, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI4502181, Length=198, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790362, Length=260, Percent_Identity=38.4615384615385, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71994009, Length=254, Percent_Identity=31.8897637795276, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992966, Length=246, Percent_Identity=29.6747967479675, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17544068, Length=283, Percent_Identity=32.1554770318021, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531429, Length=243, Percent_Identity=30.8641975308642, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17531431, Length=243, Percent_Identity=30.8641975308642, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71992961, Length=242, Percent_Identity=28.5123966942149, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32564052, Length=245, Percent_Identity=29.3877551020408, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17533613, Length=245, Percent_Identity=29.3877551020408, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558372, Length=240, Percent_Identity=27.5, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=202, Percent_Identity=38.1188118811881, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6322985, Length=305, Percent_Identity=29.5081967213115, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652747, Length=240, Percent_Identity=32.9166666666667, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI45551084, Length=240, Percent_Identity=32.9166666666667, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24762342, Length=242, Percent_Identity=30.9917355371901, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28866; Mature: 28866

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTSLATQFLGEVIGTAILIILGAGVVAGVSLKRSKAENGGWVVITLAWGLGVTMGVYV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEHHHHHHHHHHHH
SGYMSMAHLNPAVTIGMALAGAFPWNYVLPYILAQFIGAFIGATLIWLHYYPHWKQTEDK
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PTKLGVFATAPAIRHFTSNFFGEALGTFILVFGLLSLGANSFSDGLNPLVVGALIVAIGM
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHH
SLGGTTGYAINPARDLGPRIAHFVWPISGKGGSDWGYSWVPVIGPIMGGALGALGYNAII
HCCCCCCCEECCHHHHCHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHEE
NGELGMWFWVFAVLFVLILILTMQLDKKKDLA
ECCHHHHHHHHHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MIDTSLATQFLGEVIGTAILIILGAGVVAGVSLKRSKAENGGWVVITLAWGLGVTMGVYV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEHHHHHHHHHHHH
SGYMSMAHLNPAVTIGMALAGAFPWNYVLPYILAQFIGAFIGATLIWLHYYPHWKQTEDK
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PTKLGVFATAPAIRHFTSNFFGEALGTFILVFGLLSLGANSFSDGLNPLVVGALIVAIGM
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHH
SLGGTTGYAINPARDLGPRIAHFVWPISGKGGSDWGYSWVPVIGPIMGGALGALGYNAII
HCCCCCCCEECCHHHHCHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHEE
NGELGMWFWVFAVLFVLILILTMQLDKKKDLA
ECCHHHHHHHHHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]