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The map label for this gene is glpK

Identifier: 226224139

GI number: 226224139

Start: 1557052

End: 1558545

Strand: Reverse

Name: glpK

Synonym: Lm4b_01548

Alternate gene names: 226224139

Gene position: 1558545-1557052 (Counterclockwise)

Preceding gene: 226224140

Following gene: 226224138

Centisome position: 53.51

GC content: 39.42

Gene sequence:

>1494_bases
ATGGAAAAGAAATATATTTTAGCACTGGATCAAGGAACAACGAGTTCAAGAGCGATGATTATTGACGAAGAAGGAGAAGT
AATTGGCGTTGCACAAGAAGAATTTGATCAAATTTTTCCTAAACCAGGTTGGGTTGAACATAATGCCAATGAAATCTGGG
CTTCGATTTTAGCTGTTATTGCAGGCGTATTACTCAAAACAAATATTTCTTCTAAAGAAATTGCTGGGATTGGTATTACA
AACCAACGTGAAACTACTGTTATTTGGGATAAAGAAAGCGGAAATCCAATTTATAATGCGATTGTTTGGCAATCTCGTCA
AACAGAAGATATTTGTAAACAATTACGTAAAGACGGCTATGAAGATACCATTCGTTCTAAAACTGGTCTTTTGATTGATC
CGTATTTTGCGGGTACAAAAGCTCGCTGGATTCTCGACCATGTAGATGGTGCGCAAGAACGTGCTGAAAAAGGAGAACTT
CTTTTCGGAACAATCGATACTTGGTTAGTTTGGAAATTAACTGGTGGTCGTGCTCATATTACAGATTATTCCAATGCTTC
TCGGACACTTCTTTATAATATTTACGATTTAGAGTGGGATGATGAACTTCTAAAAATGCTTAATATCCCAAAAGCAATGT
TACCTGAAGTTCGTCCTTCCTCTGAAGTGTATGCGGACACAGTGCCTTATCACTTCTTTGGTGAAGAAGTGCCGGTTGCA
GGTATTGCTGGTGACCAACAAGCCGCTTTATTCGGACAAGGTTGTTTTGAAAAAGGGATGGCGAAAAATACTTATGGAAC
TGGTTGTTTCTTACTAATGAACACTGGGGAAAAAGCAGTCCGTTCTGAAAACGGCTTATTAACAACACTAGCTTGGGGAA
TTGATGGAAAAGTAGAATATGCACTTGAAGGTAGTATATTCGTTGCTGGTTCGGCTATTCAGTGGTTACGTGACGGGTTA
CGAATGGTTCGTCAATCAAGCGATTCTGAAAACTATGCGAGCCGGATTGAATCTAGTGATGGTGTATATGTTGTACCAGC
ATTCGTTGGTTTAGGCGCACCTTACTGGGATTCCGATGTTCGTGGTGCTGTTTTTGGATTAACTCGTGGTACCGAAAAAG
AACAATTTATCCGTGCGACATTGGAATCTCTTGCTTATCAAACAAGAGACGTGCTTTATGCAATGGAACAAGATTCTGGT
ATTAGCTTAAAAACGTTACGTGTGGATGGCGGGGCTTCTGCTAATAACTTCTTAATGCAATTCCAATCCGATATTCTAGG
CGTTCCGGTAGAACGTCCAGAAAACAAAGAAACAACCGTTCTAGGTGCGGCATTCTTAGCTGGACTTGCGGTAGGCGTTT
GGAAAGACAAAAACGAAATTAAAAAACATTGGAAACTAGACAAACGTTTTGAAGTAGAAATGAAAGATGAACAAAGAGAA
GATTTATATGAAGGTTGGCATAAAGCTGTCAAAGCTGCACAAGCCTTTAAATAA

Upstream 100 bases:

>100_bases
TCGACAAGAAAAAAGATTTAGCATAAGCTAGAATAATAACTAGCTCCAAGCGAAGGTCTTCGGGCTTTCGTTGTACTTAA
AACTAGAGGAGGAATTTTAC

Downstream 100 bases:

>100_bases
AATTTTAAGATGCGCCAAATGTGTAAAAGCATTTGGCGCTTTTTTGTTATGTAAAACAAATAAATATTTGGAAATAGAAA
TAAATATAGTGACAGATGTA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MEKKYILALDQGTTSSRAMIIDEEGEVIGVAQEEFDQIFPKPGWVEHNANEIWASILAVIAGVLLKTNISSKEIAGIGIT
NQRETTVIWDKESGNPIYNAIVWQSRQTEDICKQLRKDGYEDTIRSKTGLLIDPYFAGTKARWILDHVDGAQERAEKGEL
LFGTIDTWLVWKLTGGRAHITDYSNASRTLLYNIYDLEWDDELLKMLNIPKAMLPEVRPSSEVYADTVPYHFFGEEVPVA
GIAGDQQAALFGQGCFEKGMAKNTYGTGCFLLMNTGEKAVRSENGLLTTLAWGIDGKVEYALEGSIFVAGSAIQWLRDGL
RMVRQSSDSENYASRIESSDGVYVVPAFVGLGAPYWDSDVRGAVFGLTRGTEKEQFIRATLESLAYQTRDVLYAMEQDSG
ISLKTLRVDGGASANNFLMQFQSDILGVPVERPENKETTVLGAAFLAGLAVGVWKDKNEIKKHWKLDKRFEVEMKDEQRE
DLYEGWHKAVKAAQAFK

Sequences:

>Translated_497_residues
MEKKYILALDQGTTSSRAMIIDEEGEVIGVAQEEFDQIFPKPGWVEHNANEIWASILAVIAGVLLKTNISSKEIAGIGIT
NQRETTVIWDKESGNPIYNAIVWQSRQTEDICKQLRKDGYEDTIRSKTGLLIDPYFAGTKARWILDHVDGAQERAEKGEL
LFGTIDTWLVWKLTGGRAHITDYSNASRTLLYNIYDLEWDDELLKMLNIPKAMLPEVRPSSEVYADTVPYHFFGEEVPVA
GIAGDQQAALFGQGCFEKGMAKNTYGTGCFLLMNTGEKAVRSENGLLTTLAWGIDGKVEYALEGSIFVAGSAIQWLRDGL
RMVRQSSDSENYASRIESSDGVYVVPAFVGLGAPYWDSDVRGAVFGLTRGTEKEQFIRATLESLAYQTRDVLYAMEQDSG
ISLKTLRVDGGASANNFLMQFQSDILGVPVERPENKETTVLGAAFLAGLAVGVWKDKNEIKKHWKLDKRFEVEMKDEQRE
DLYEGWHKAVKAAQAFK
>Mature_497_residues
MEKKYILALDQGTTSSRAMIIDEEGEVIGVAQEEFDQIFPKPGWVEHNANEIWASILAVIAGVLLKTNISSKEIAGIGIT
NQRETTVIWDKESGNPIYNAIVWQSRQTEDICKQLRKDGYEDTIRSKTGLLIDPYFAGTKARWILDHVDGAQERAEKGEL
LFGTIDTWLVWKLTGGRAHITDYSNASRTLLYNIYDLEWDDELLKMLNIPKAMLPEVRPSSEVYADTVPYHFFGEEVPVA
GIAGDQQAALFGQGCFEKGMAKNTYGTGCFLLMNTGEKAVRSENGLLTTLAWGIDGKVEYALEGSIFVAGSAIQWLRDGL
RMVRQSSDSENYASRIESSDGVYVVPAFVGLGAPYWDSDVRGAVFGLTRGTEKEQFIRATLESLAYQTRDVLYAMEQDSG
ISLKTLRVDGGASANNFLMQFQSDILGVPVERPENKETTVLGAAFLAGLAVGVWKDKNEIKKHWKLDKRFEVEMKDEQRE
DLYEGWHKAVKAAQAFK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=500, Percent_Identity=46.2, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI189409120, Length=496, Percent_Identity=46.3709677419355, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI4504007, Length=496, Percent_Identity=46.3709677419355, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=45.9677419354839, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI88196792, Length=504, Percent_Identity=34.3253968253968, Blast_Score=311, Evalue=1e-84,
Organism=Escherichia coli, GI1790361, Length=496, Percent_Identity=63.3064516129032, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=505, Percent_Identity=24.1584158415842, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787789, Length=515, Percent_Identity=24.6601941747573, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789987, Length=493, Percent_Identity=23.9350912778905, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789168, Length=483, Percent_Identity=21.9461697722567, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=449, Percent_Identity=41.6481069042316, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71986140, Length=525, Percent_Identity=25.1428571428571, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71986147, Length=301, Percent_Identity=28.9036544850498, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6321755, Length=550, Percent_Identity=36.1818181818182, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI17864214, Length=468, Percent_Identity=44.6581196581197, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655555, Length=493, Percent_Identity=39.553752535497, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24655560, Length=493, Percent_Identity=39.553752535497, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24655570, Length=493, Percent_Identity=39.553752535497, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24655574, Length=493, Percent_Identity=39.553752535497, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24655565, Length=493, Percent_Identity=39.553752535497, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24654573, Length=393, Percent_Identity=47.8371501272265, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=521, Percent_Identity=32.6295585412668, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24652871, Length=488, Percent_Identity=32.9918032786885, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24656367, Length=508, Percent_Identity=28.3464566929134, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI21357867, Length=523, Percent_Identity=27.5334608030593, Blast_Score=238, Evalue=9e-63,
Organism=Drosophila melanogaster, GI24656372, Length=343, Percent_Identity=29.7376093294461, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI45552909, Length=285, Percent_Identity=27.3684210526316, Blast_Score=125, Evalue=9e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55449; Mature: 55449

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKYILALDQGTTSSRAMIIDEEGEVIGVAQEEFDQIFPKPGWVEHNANEIWASILAVI
CCCCEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
AGVLLKTNISSKEIAGIGITNQRETTVIWDKESGNPIYNAIVWQSRQTEDICKQLRKDGY
HHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCC
EDTIRSKTGLLIDPYFAGTKARWILDHVDGAQERAEKGELLFGTIDTWLVWKLTGGRAHI
HHHHHCCCCCEEECCCCCCCCEEEEECCCCHHHHHHCCCEEEEEECEEEEEEEECCEEEE
TDYSNASRTLLYNIYDLEWDDELLKMLNIPKAMLPEVRPSSEVYADTVPYHFFGEEVPVA
ECCCCCCCEEEEEEEECCCHHHHHHHHCCCHHHCCCCCCCCCCEECCCCEEECCCCCCEE
GIAGDQQAALFGQGCFEKGMAKNTYGTGCFLLMNTGEKAVRSENGLLTTLAWGIDGKVEY
ECCCCCCHHHHHCHHHHHCCCCCCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCCEEEE
ALEGSIFVAGSAIQWLRDGLRMVRQSSDSENYASRIESSDGVYVVPAFVGLGAPYWDSDV
EECCCEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEHHHHCCCCCCCCCC
RGAVFGLTRGTEKEQFIRATLESLAYQTRDVLYAMEQDSGISLKTLRVDGGASANNFLMQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCHHHHHHH
FQSDILGVPVERPENKETTVLGAAFLAGLAVGVWKDKNEIKKHWKLDKRFEVEMKDEQRE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCHHH
DLYEGWHKAVKAAQAFK
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKKYILALDQGTTSSRAMIIDEEGEVIGVAQEEFDQIFPKPGWVEHNANEIWASILAVI
CCCCEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
AGVLLKTNISSKEIAGIGITNQRETTVIWDKESGNPIYNAIVWQSRQTEDICKQLRKDGY
HHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCC
EDTIRSKTGLLIDPYFAGTKARWILDHVDGAQERAEKGELLFGTIDTWLVWKLTGGRAHI
HHHHHCCCCCEEECCCCCCCCEEEEECCCCHHHHHHCCCEEEEEECEEEEEEEECCEEEE
TDYSNASRTLLYNIYDLEWDDELLKMLNIPKAMLPEVRPSSEVYADTVPYHFFGEEVPVA
ECCCCCCCEEEEEEEECCCHHHHHHHHCCCHHHCCCCCCCCCCEECCCCEEECCCCCCEE
GIAGDQQAALFGQGCFEKGMAKNTYGTGCFLLMNTGEKAVRSENGLLTTLAWGIDGKVEY
ECCCCCCHHHHHCHHHHHCCCCCCCCCEEEEEEECCCHHHHCCCCEEEEEEECCCCEEEE
ALEGSIFVAGSAIQWLRDGLRMVRQSSDSENYASRIESSDGVYVVPAFVGLGAPYWDSDV
EECCCEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEHHHHCCCCCCCCCC
RGAVFGLTRGTEKEQFIRATLESLAYQTRDVLYAMEQDSGISLKTLRVDGGASANNFLMQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCHHHHHHH
FQSDILGVPVERPENKETTVLGAAFLAGLAVGVWKDKNEIKKHWKLDKRFEVEMKDEQRE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCHHH
DLYEGWHKAVKAAQAFK
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]