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The map label for this gene is ruvA

Identifier: 226224134

GI number: 226224134

Start: 1552119

End: 1552724

Strand: Reverse

Name: ruvA

Synonym: Lm4b_01543

Alternate gene names: 226224134

Gene position: 1552724-1552119 (Counterclockwise)

Preceding gene: 226224135

Following gene: 226224133

Centisome position: 53.31

GC content: 38.12

Gene sequence:

>606_bases
TTGTACGATTACATAAAAGGAACCGTTACGACGATTACACCCGAATATATTGTTGTCGAGGCAGGACAAATCGGCTATCA
AATAATTACAGGAAACCCGTTTTCCTTTCAACGATTAGAAGGTACAGAAGCACAAGTCTTTTTGTATCAACATGTGCGGG
AGGATAATATTTCTCTATTCGGTTTTCAAACAACAGAAGAACGTTATTTATTCAAAAAATTATTGAGTGTTTCGGGCATT
GGACCAAAAAGCGCGCTAGCTATTATTGCTTCAGGCGATGTCGTTCCACTGATTTCTGCTATTGAATCCGAAGACGATGT
TTATTTAACCAAATTTCCAAGCGTTGGTAAAAAAACAGCACGCCAAATCATTCTTGATTTGAAAGGCAAACTAGCTGATG
TTGTCGCGAGCGAAATTGTTTATGTTGCTCCAGAAAACGATATGGTTGCTGGACTTTCTCCGCAATTAGAAGAAGCTGTA
TTAGCTTTAGAAGCGCTCGGTTATAGCACGCGAGAACTAAAAAAAGTAATACCAAAATTGTCAAAAGAAGAAGACCTAAC
AAGCGACGCATATATCAAGCTAGCACTACAATTAATGACAAAATAG

Upstream 100 bases:

>100_bases
AACTAAATAAGCGTTTCTTTTATAAACAAAAACTAGTACAGACGTTCCGTTTTATGCTATCATATAATGTATGACGTGAA
ATGAAGAGAGGACGAATAAA

Downstream 100 bases:

>100_bases
AGAGGAGCTTGTGGCATGGATGAACGAATTATTTCAAGTGAAACGGTAGACGCAGAAGAAGTATCTTTTGAAACTAGTTT
GCGGCCACAGAACCTTTCAC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MYDYIKGTVTTITPEYIVVEAGQIGYQIITGNPFSFQRLEGTEAQVFLYQHVREDNISLFGFQTTEERYLFKKLLSVSGI
GPKSALAIIASGDVVPLISAIESEDDVYLTKFPSVGKKTARQIILDLKGKLADVVASEIVYVAPENDMVAGLSPQLEEAV
LALEALGYSTRELKKVIPKLSKEEDLTSDAYIKLALQLMTK

Sequences:

>Translated_201_residues
MYDYIKGTVTTITPEYIVVEAGQIGYQIITGNPFSFQRLEGTEAQVFLYQHVREDNISLFGFQTTEERYLFKKLLSVSGI
GPKSALAIIASGDVVPLISAIESEDDVYLTKFPSVGKKTARQIILDLKGKLADVVASEIVYVAPENDMVAGLSPQLEEAV
LALEALGYSTRELKKVIPKLSKEEDLTSDAYIKLALQLMTK
>Mature_201_residues
MYDYIKGTVTTITPEYIVVEAGQIGYQIITGNPFSFQRLEGTEAQVFLYQHVREDNISLFGFQTTEERYLFKKLLSVSGI
GPKSALAIIASGDVVPLISAIESEDDVYLTKFPSVGKKTARQIILDLKGKLADVVASEIVYVAPENDMVAGLSPQLEEAV
LALEALGYSTRELKKVIPKLSKEEDLTSDAYIKLALQLMTK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family [H]

Homologues:

Organism=Escherichia coli, GI1788168, Length=204, Percent_Identity=32.3529411764706, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994 [H]

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 22157; Mature: 22157

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDYIKGTVTTITPEYIVVEAGQIGYQIITGNPFSFQRLEGTEAQVFLYQHVREDNISLF
CCCCCCCEEEEECCCEEEEECCCCCEEEEECCCCCEEECCCCCCEEEEEEHHCCCCEEEE
GFQTTEERYLFKKLLSVSGIGPKSALAIIASGDVVPLISAIESEDDVYLTKFPSVGKKTA
EECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEECCCCCHHHH
RQIILDLKGKLADVVASEIVYVAPENDMVAGLSPQLEEAVLALEALGYSTRELKKVIPKL
HHHHHHHHHHHHHHHHHCEEEECCCCCEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHC
SKEEDLTSDAYIKLALQLMTK
CCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MYDYIKGTVTTITPEYIVVEAGQIGYQIITGNPFSFQRLEGTEAQVFLYQHVREDNISLF
CCCCCCCEEEEECCCEEEEECCCCCEEEEECCCCCEEECCCCCCEEEEEEHHCCCCEEEE
GFQTTEERYLFKKLLSVSGIGPKSALAIIASGDVVPLISAIESEDDVYLTKFPSVGKKTA
EECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEECCCCCHHHH
RQIILDLKGKLADVVASEIVYVAPENDMVAGLSPQLEEAVLALEALGYSTRELKKVIPKL
HHHHHHHHHHHHHHHHHCEEEECCCCCEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHC
SKEEDLTSDAYIKLALQLMTK
CCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]