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The map label for this gene is yrvJ [H]

Identifier: 226224122

GI number: 226224122

Start: 1538058

End: 1539341

Strand: Direct

Name: yrvJ [H]

Synonym: Lm4b_01531b

Alternate gene names: 226224122

Gene position: 1538058-1539341 (Clockwise)

Preceding gene: 226224119

Following gene: 226224179

Centisome position: 52.81

GC content: 40.73

Gene sequence:

>1284_bases
ATGAAGAATAAATTCATTTTTATCACCGTTGTCTCCATTTTATTGATTGCAGCAGGTATTTTTACAACCATAGCAATGGC
GAACGCGAATTCCGTAGTCGTCAAAGCAGAAGTCTTGAATGTCCGCAGCGGTCCTGGTTTAGCATATGATGTAACGAGCC
AAGCCAGAAAAAATGAAGTACTCCGGGTAGTCGGTGAAGAAAATCAATGGTACAAAGTTCAGTTAGATAACGGAAATAGC
GGTTGGGTTGCAAGCTGGTTAGTAGAAAACACTGATGTCAGCGCAGCAAGTAACAGTGTCGCTATCGTTTCTTCTGACGG
CGGTTTGAATGTACGTGAAAAACCAAGTACCTCAAGTAAAGCACTTGGATTGCTAAATAATGGTGATCAAGTAACAGTTA
CTAGCCAACAAAACGGCTGGGCGCAAATCCAATATAACGGTACAAGCGCATGGGTTAGCTCCGATTACTTAACGATTCGT
GAATCTGTTACAAAAGTAGATGAAAGTGAGCTACAAACAGTAACCATTCGCGATGACTCCACTAATATTAGAAATAAGCC
AAGTCGTGATGGCGCCGTCATCGAAAAAGCAAATTCTGGTCAAGGGTTCGCTATTCAAGGAGTACAAGGTGACTGGTATA
AGATACGTACGACAAGCGGCGAAGAAGGTTATGTAGCTAACTGGGTAGTCGATGTTTCTGACAAAGGTCAAACATCCAGC
CCTCGAAGCAAAACAACCAAATTATCCGAAGCAACCATCGTTATTGACCCCGGACACGGTGGCAACGATCCGGGCGCTAA
AGGGTCAAATGGCACAATTGAAAAAGAAATGACTTTGAAGACAGCAAAACAATTAAAACAAAAATTAGAAGCTAGAGGCG
CTAAAGTAATCCTAACGAGAAACAGTGATAAATATGTCTCTCTAAAAGGAAGAACAAATATAGCTGCCGAAAATAATGCA
GATGTGTTTATTAGTATTCACTTTGATAGCTTAGAAGACGCGAGCAAAGGGGTAAGCGGGCAAACAACTTATTATTATGA
TAATAGTGATAAATCGCTTGCTGAAAGTATTAATACTACGCTCGGGAAAGACCTCCCTACTACTAATCGTGGTGCAAGAG
TCGGCGATTATTATGTAGTCAGAGAAAATTCTCAACCAGCAGTCCTTCTAGAACTTGGTTATCTAAGTTCTGCAAAAGAT
GAAAGTAATATCAACTCAGCATCATACAGAAGCCAAATCGCTGAATCAGTAACAGATGGCCTATCTAATTACTTCTCTAA
TTAA

Upstream 100 bases:

>100_bases
TTTATAAAAAACCACTTTCTAGTTCCATGAAAGCGCATTTTATGGTAACATTTTACTGTCAGATAAAAGTTTATGAGTAT
ATGGGTGGTGTTAGCTTTAA

Downstream 100 bases:

>100_bases
AAAAACAATCATTCAGGAGACTGAATGATTGTTTTTTTATTTACGCATTTCATCTGAATCGAGCATTATTGTTACTGGAC
CGTGATTGACAATTTTCACA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKNKFIFITVVSILLIAAGIFTTIAMANANSVVVKAEVLNVRSGPGLAYDVTSQARKNEVLRVVGEENQWYKVQLDNGNS
GWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSKALGLLNNGDQVTVTSQQNGWAQIQYNGTSAWVSSDYLTIR
ESVTKVDESELQTVTIRDDSTNIRNKPSRDGAVIEKANSGQGFAIQGVQGDWYKIRTTSGEEGYVANWVVDVSDKGQTSS
PRSKTTKLSEATIVIDPGHGGNDPGAKGSNGTIEKEMTLKTAKQLKQKLEARGAKVILTRNSDKYVSLKGRTNIAAENNA
DVFISIHFDSLEDASKGVSGQTTYYYDNSDKSLAESINTTLGKDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSAKD
ESNINSASYRSQIAESVTDGLSNYFSN

Sequences:

>Translated_427_residues
MKNKFIFITVVSILLIAAGIFTTIAMANANSVVVKAEVLNVRSGPGLAYDVTSQARKNEVLRVVGEENQWYKVQLDNGNS
GWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSKALGLLNNGDQVTVTSQQNGWAQIQYNGTSAWVSSDYLTIR
ESVTKVDESELQTVTIRDDSTNIRNKPSRDGAVIEKANSGQGFAIQGVQGDWYKIRTTSGEEGYVANWVVDVSDKGQTSS
PRSKTTKLSEATIVIDPGHGGNDPGAKGSNGTIEKEMTLKTAKQLKQKLEARGAKVILTRNSDKYVSLKGRTNIAAENNA
DVFISIHFDSLEDASKGVSGQTTYYYDNSDKSLAESINTTLGKDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSAKD
ESNINSASYRSQIAESVTDGLSNYFSN
>Mature_427_residues
MKNKFIFITVVSILLIAAGIFTTIAMANANSVVVKAEVLNVRSGPGLAYDVTSQARKNEVLRVVGEENQWYKVQLDNGNS
GWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSKALGLLNNGDQVTVTSQQNGWAQIQYNGTSAWVSSDYLTIR
ESVTKVDESELQTVTIRDDSTNIRNKPSRDGAVIEKANSGQGFAIQGVQGDWYKIRTTSGEEGYVANWVVDVSDKGQTSS
PRSKTTKLSEATIVIDPGHGGNDPGAKGSNGTIEKEMTLKTAKQLKQKLEARGAKVILTRNSDKYVSLKGRTNIAAENNA
DVFISIHFDSLEDASKGVSGQTTYYYDNSDKSLAESINTTLGKDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSAKD
ESNINSASYRSQIAESVTDGLSNYFSN

Specific function: Probably involved in cell-wall metabolism [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=221, Percent_Identity=26.2443438914027, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=28.8888888888889, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788776, Length=232, Percent_Identity=26.7241379310345, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR017293
- InterPro:   IPR003646
- InterPro:   IPR013247 [H]

Pfam domain/function: PF01520 Amidase_3; PF08239 SH3_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46044; Mature: 46044

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKFIFITVVSILLIAAGIFTTIAMANANSVVVKAEVLNVRSGPGLAYDVTSQARKNEV
CCCCEEEHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCCCCEEEECCCHHHCCCE
LRVVGEENQWYKVQLDNGNSGWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSK
EEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
ALGLLNNGDQVTVTSQQNGWAQIQYNGTSAWVSSDYLTIRESVTKVDESELQTVTIRDDS
EEEEECCCCEEEEEECCCCEEEEEECCCEEEEECCCEEEHHHHHHCCCCCEEEEEEECCC
TNIRNKPSRDGAVIEKANSGQGFAIQGVQGDWYKIRTTSGEEGYVANWVVDVSDKGQTSS
CCCCCCCCCCCCEEEECCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCC
PRSKTTKLSEATIVIDPGHGGNDPGAKGSNGTIEKEMTLKTAKQLKQKLEARGAKVILTR
CCCCCEEECCEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEE
NSDKYVSLKGRTNIAAENNADVFISIHFDSLEDASKGVSGQTTYYYDNSDKSLAESINTT
CCCCEEEEECCCEEEECCCCEEEEEEEECCCHHHHCCCCCCEEEEEECCCHHHHHHHHHH
LGKDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSAKDESNINSASYRSQIAESVTDG
HCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSNYFSN
HHHHHCC
>Mature Secondary Structure
MKNKFIFITVVSILLIAAGIFTTIAMANANSVVVKAEVLNVRSGPGLAYDVTSQARKNEV
CCCCEEEHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCCCCEEEECCCHHHCCCE
LRVVGEENQWYKVQLDNGNSGWVASWLVENTDVSAASNSVAIVSSDGGLNVREKPSTSSK
EEEECCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
ALGLLNNGDQVTVTSQQNGWAQIQYNGTSAWVSSDYLTIRESVTKVDESELQTVTIRDDS
EEEEECCCCEEEEEECCCCEEEEEECCCEEEEECCCEEEHHHHHHCCCCCEEEEEEECCC
TNIRNKPSRDGAVIEKANSGQGFAIQGVQGDWYKIRTTSGEEGYVANWVVDVSDKGQTSS
CCCCCCCCCCCCEEEECCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCC
PRSKTTKLSEATIVIDPGHGGNDPGAKGSNGTIEKEMTLKTAKQLKQKLEARGAKVILTR
CCCCCEEECCEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEE
NSDKYVSLKGRTNIAAENNADVFISIHFDSLEDASKGVSGQTTYYYDNSDKSLAESINTT
CCCCEEEEECCCEEEECCCCEEEEEEEECCCHHHHCCCCCCEEEEEECCCHHHHHHHHHH
LGKDLPTTNRGARVGDYYVVRENSQPAVLLELGYLSSAKDESNINSASYRSQIAESVTDG
HCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSNYFSN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]