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The map label for this gene is hisS

Identifier: 226224121

GI number: 226224121

Start: 1536330

End: 1537607

Strand: Reverse

Name: hisS

Synonym: Lm4b_01530

Alternate gene names: 226224121

Gene position: 1537607-1536330 (Counterclockwise)

Preceding gene: 226224123

Following gene: 226224120

Centisome position: 52.79

GC content: 37.87

Gene sequence:

>1278_bases
ATGGATTTGCAATTACCGAGAGGGACACGAGATATTTTGCCAGAAGAGGTTAGTAAATGGCATTTTTTAGAGACAGAGTT
TAAAAAAGTGTGTGAGAACTATCAATATGAAGAAATTAGAACACCTGTTTTTGAACATACAGAATTATTTGAACGCGGGG
TTGGTGATTCCACCGATATCGTATCAAAAGAAATGTACACTTTCCAAGATAAGGGTGGCAGAAGTTTAACGCTGAGACCA
GAGGGGACCGCTTCTGTTGTTCGTGCTTTTGTTGAGCATAAATTATACGGCGAAGTAAGCCAACCAATCAAAATGTATTA
TAATGAGCCAATGTTTCGTTATGAACGACCACAAGGCGGAAGACAACGCCAGTTTACTCAAATGGGCATTGAGGCGCTTG
GAAGTGATGATCCTTCTATTGATGTAGAAGTAATTTCACTAGCAATGGAGTTTTTCCGGAAAATTGGTCTAACAAACATT
AAATTGGTCATTAATAGTCTTGGTGATAAAGAAAGTCGTCTAAAACACCGGGAAGCCCTGGTTGCGCATTTTGAACCACA
TATTGATGAATTTTGTGCGGAATGCCAAGTGCGGTTACACAAAAATCCACTGCGTATTTTAGACTGTAAAAAAGATCATG
ACAATCCACTAATTCAATCGGCACCATCTATTCTGGACTTTTTAAATGAAGAATCTGTTGCCTATTTTGAAAATGTAAAA
AAATACTTAAATGCGCTAGAGATTCCGTTTGAAATTGATTCAACGATGGTTCGTGGTTTAGATTATTATAACCATACGAC
ATTTGAAATTATGAGCGTAGAAGAAGGATTTGGTGCGAAAACAACGCTTTGTGGTGGCGGAAGATATCACGGTTTAGTGA
GAGAGTTTGGTGGCCCAGATACACCAGGAATGGGCTTTGGTATTGGTGTGGAACGTATTTTACTGGCGCTAGAAAAAGCG
GACATCAAAATACCTGCGAAAAAACCGTTAGAGGTTTATGTGATTACAGCGCAACCGGAAGCAGAACTAAAAGCCGTAAC
ACTCGTAAATAAATTAAGACAAAACGGCATTAGTGCAGAAAAAGATTACTTAAAACGTAAATTCAAAGCACAACTAAAAG
ACGCCAATAGAAAAAATGCCGTATATACCATTATTCTTGGTGAAGAAGAATTACAAACAGGCAATTATCAATTGAAAAAT
ATGGAAACAGGCGAGCAAGAAGCTGTGTCTGAAACAACTATACTAGAAAAATTAATAAACACCAAGGGGGAGAATTAA

Upstream 100 bases:

>100_bases
GTGGTACCACGGGTGTAAACAAACTCTCGTCCCTTGTTTTTTATAACAAGTGACGGGGGTTTTTCTTTATATTTTGAAGT
GAAAAAGGAGGCTTATGGTG

Downstream 100 bases:

>100_bases
AATGGAAAAACGTACAAGTTACTGCGGTGAATTAAACGAAGCGCATATTGGTCAAAGTGTTGTCCTTCATGGGTGGGTGC
AAAAAAGACGCGACTTAGGT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MDLQLPRGTRDILPEEVSKWHFLETEFKKVCENYQYEEIRTPVFEHTELFERGVGDSTDIVSKEMYTFQDKGGRSLTLRP
EGTASVVRAFVEHKLYGEVSQPIKMYYNEPMFRYERPQGGRQRQFTQMGIEALGSDDPSIDVEVISLAMEFFRKIGLTNI
KLVINSLGDKESRLKHREALVAHFEPHIDEFCAECQVRLHKNPLRILDCKKDHDNPLIQSAPSILDFLNEESVAYFENVK
KYLNALEIPFEIDSTMVRGLDYYNHTTFEIMSVEEGFGAKTTLCGGGRYHGLVREFGGPDTPGMGFGIGVERILLALEKA
DIKIPAKKPLEVYVITAQPEAELKAVTLVNKLRQNGISAEKDYLKRKFKAQLKDANRKNAVYTIILGEEELQTGNYQLKN
METGEQEAVSETTILEKLINTKGEN

Sequences:

>Translated_425_residues
MDLQLPRGTRDILPEEVSKWHFLETEFKKVCENYQYEEIRTPVFEHTELFERGVGDSTDIVSKEMYTFQDKGGRSLTLRP
EGTASVVRAFVEHKLYGEVSQPIKMYYNEPMFRYERPQGGRQRQFTQMGIEALGSDDPSIDVEVISLAMEFFRKIGLTNI
KLVINSLGDKESRLKHREALVAHFEPHIDEFCAECQVRLHKNPLRILDCKKDHDNPLIQSAPSILDFLNEESVAYFENVK
KYLNALEIPFEIDSTMVRGLDYYNHTTFEIMSVEEGFGAKTTLCGGGRYHGLVREFGGPDTPGMGFGIGVERILLALEKA
DIKIPAKKPLEVYVITAQPEAELKAVTLVNKLRQNGISAEKDYLKRKFKAQLKDANRKNAVYTIILGEEELQTGNYQLKN
METGEQEAVSETTILEKLINTKGEN
>Mature_425_residues
MDLQLPRGTRDILPEEVSKWHFLETEFKKVCENYQYEEIRTPVFEHTELFERGVGDSTDIVSKEMYTFQDKGGRSLTLRP
EGTASVVRAFVEHKLYGEVSQPIKMYYNEPMFRYERPQGGRQRQFTQMGIEALGSDDPSIDVEVISLAMEFFRKIGLTNI
KLVINSLGDKESRLKHREALVAHFEPHIDEFCAECQVRLHKNPLRILDCKKDHDNPLIQSAPSILDFLNEESVAYFENVK
KYLNALEIPFEIDSTMVRGLDYYNHTTFEIMSVEEGFGAKTTLCGGGRYHGLVREFGGPDTPGMGFGIGVERILLALEKA
DIKIPAKKPLEVYVITAQPEAELKAVTLVNKLRQNGISAEKDYLKRKFKAQLKDANRKNAVYTIILGEEELQTGNYQLKN
METGEQEAVSETTILEKLINTKGEN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=465, Percent_Identity=26.0215053763441, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI15029520, Length=451, Percent_Identity=23.9467849223947, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788861, Length=420, Percent_Identity=39.7619047619048, Blast_Score=338, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI71993693, Length=476, Percent_Identity=24.7899159663866, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71993686, Length=476, Percent_Identity=24.7899159663866, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24643061, Length=466, Percent_Identity=26.1802575107296, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48489; Mature: 48489

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00485 A_DEAMINASE ; PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLQLPRGTRDILPEEVSKWHFLETEFKKVCENYQYEEIRTPVFEHTELFERGVGDSTDI
CCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHH
VSKEMYTFQDKGGRSLTLRPEGTASVVRAFVEHKLYGEVSQPIKMYYNEPMFRYERPQGG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
RQRQFTQMGIEALGSDDPSIDVEVISLAMEFFRKIGLTNIKLVINSLGDKESRLKHREAL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
VAHFEPHIDEFCAECQVRLHKNPLRILDCKKDHDNPLIQSAPSILDFLNEESVAYFENVK
HHHHCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
KYLNALEIPFEIDSTMVRGLDYYNHTTFEIMSVEEGFGAKTTLCGGGRYHGLVREFGGPD
HHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCC
TPGMGFGIGVERILLALEKADIKIPAKKPLEVYVITAQPEAELKAVTLVNKLRQNGISAE
CCCCCCHHHHHHHHHHHHHCCEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHH
KDYLKRKFKAQLKDANRKNAVYTIILGEEELQTGNYQLKNMETGEQEAVSETTILEKLIN
HHHHHHHHHHHHHCCCCCCCEEEEEECHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
TKGEN
CCCCC
>Mature Secondary Structure
MDLQLPRGTRDILPEEVSKWHFLETEFKKVCENYQYEEIRTPVFEHTELFERGVGDSTDI
CCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHH
VSKEMYTFQDKGGRSLTLRPEGTASVVRAFVEHKLYGEVSQPIKMYYNEPMFRYERPQGG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
RQRQFTQMGIEALGSDDPSIDVEVISLAMEFFRKIGLTNIKLVINSLGDKESRLKHREAL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
VAHFEPHIDEFCAECQVRLHKNPLRILDCKKDHDNPLIQSAPSILDFLNEESVAYFENVK
HHHHCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
KYLNALEIPFEIDSTMVRGLDYYNHTTFEIMSVEEGFGAKTTLCGGGRYHGLVREFGGPD
HHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCC
TPGMGFGIGVERILLALEKADIKIPAKKPLEVYVITAQPEAELKAVTLVNKLRQNGISAE
CCCCCCHHHHHHHHHHHHHCCEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCHH
KDYLKRKFKAQLKDANRKNAVYTIILGEEELQTGNYQLKNMETGEQEAVSETTILEKLIN
HHHHHHHHHHHHHCCCCCCCEEEEEECHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
TKGEN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]