Click here to switch to the map view.

The map label for this gene is aspS

Identifier: 226224120

GI number: 226224120

Start: 1534553

End: 1536328

Strand: Reverse

Name: aspS

Synonym: Lm4b_01529

Alternate gene names: 226224120

Gene position: 1536328-1534553 (Counterclockwise)

Preceding gene: 226224121

Following gene: 226224115

Centisome position: 52.75

GC content: 39.19

Gene sequence:

>1776_bases
ATGGAAAAACGTACAAGTTACTGCGGTGAATTAAACGAAGCGCATATTGGTCAAAGTGTTGTCCTTCATGGGTGGGTGCA
AAAAAGACGCGACTTAGGTGGTTTAATTTTTATTGATTTACGTGATCGCGAAGGAATTGTCCAAGTTGTTTTCAATCCTG
AATTTTCGAAAGAAGCGCTTGAAATCGCTGATAGTGTTCGAAATGAATTTGTTGTTACTATTAAAGGGACAGTCCATGCT
CGTGGTGAAAAAGCTATCAATGAAAAATTAGCAACTGGTAAAGTAGAAGTTTTAGCAGAAGAAATTACCATTTTAAATTC
GTCTAAAACACCACCATTTTATATTGAAGATGGTGTGAATGTTTCGGATGAATTGCGTTTGAAATACCGTTATTTAGACT
TACGACGCCCGGAAATGAACAACATTTTCAAAATGCGTCACACGGTAACGAGAACGTTCCGCAATAAGCTGGATGCACTC
GGATTCTTTGATATTGAAACACCTTATTTAACGAAAAGTACGCCAGAAGGTGCACGCGATTATCTTGTACCAAGTCGTGT
TTACCCTGGTAACTTCTATGCATTGCCACAATCTCCGCAAATTTTAAAACAGTTACTAATGACTGCTGGGTTTGATAAAT
ATTACCAAATCGTACGTTGCTTCCGCGATGAGGATTTGCGCGGTGACCGTCAACCTGAATTTACACAAATCGATTTAGAG
ACTAGCTTTTTAACGAAAGAAGAAATCCAAGCGATTACGGAAGATATGTTAGTAGATGTAGTGAAAGAAGCGAAAAATAT
TACTATCGACAAACCGTTTCCTCGTATGACATATAAAGAAGCAATGGATCGTTTTGGCAGTGATAAACCAGATATTCGTT
TTGGTTTAGAATTACAAAATGTATCCGATGTCGTAAAAGATGTTGATTTTAAAGTTTTCCAATCCGCGATCGAAAATGGT
GGAGAAGTGAAAGCAATCAATGCAAAAGCAGCCGCAGCTAATTTTTCACGCAAAGATTTAGATGCACTAGGTGTTTTTGT
TGCTAACTACGGGGCAAAAGGTCTTGCTTGGTTAAAAGTAGAGGCTGGTGAACTGAAAGGCCCGATTGCTAAATTCTTCC
CTGAAGAAAAAGCAACAGAACTAAAAGCATCTCTACAAGCAGAAGATGGCGATTTATTACTATTTGCAGCTGATAAAGCA
GATATCGTTGCAGCATCACTGGGGGCGCTTCGTAATAAACTTGGTAAAGAGTTAAACTTAATCAATGAAGAAGAACTTGC
TTTCTTATGGGTGACTGATTGGCCGTTATTTGAATATGATGAAGAAGCAGGGCGCTATGTATCTGCGCATCATCCATTCA
CTTTACCAAAAGAAGAAGATATTCCACTTCTTGAAACGGACTCTTCTAAAGTAATGGCAGAAGCTTATGATATCGTATTA
AATGGATATGAAATCGGCGGAGGTTCATTACGGATTTATAAAAAAGAAGTGCAAGAATCGATGTTCCGTGCACTTGGCTT
TACGGATGAATCAGCGAAAGAACAATTTGGCTTCCTAATGGATGCGTTAGAATATGGTACACCGCCTCATGGTGGTATCG
CGCTTGGATTAGATCGTATCGTTATGATCTTAGCTGGCCGAAATAACTTGCGTGACACAATAGCATTCCCTAAAACAGGT
AGTGCAGTGGATCCGCTAACAAATGCACCAGGTGAAGTGAGTGAAGCACAGCTAGCAGAGTTGAAACTCGAGACAGTGAA
AAAAGAAACAAACTAA

Upstream 100 bases:

>100_bases
GGCAATTATCAATTGAAAAATATGGAAACAGGCGAGCAAGAAGCTGTGTCTGAAACAACTATACTAGAAAAATTAATAAA
CACCAAGGGGGAGAATTAAA

Downstream 100 bases:

>100_bases
AAAAAGAAGGTGAGCGCAAATGCTCACCTTCTTTTCGTTTTAAAAATCATCACTTAATTCTTGAATGTTTTCAACGTGTA
CTTTATTAACTAATAACCCT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MEKRTSYCGELNEAHIGQSVVLHGWVQKRRDLGGLIFIDLRDREGIVQVVFNPEFSKEALEIADSVRNEFVVTIKGTVHA
RGEKAINEKLATGKVEVLAEEITILNSSKTPPFYIEDGVNVSDELRLKYRYLDLRRPEMNNIFKMRHTVTRTFRNKLDAL
GFFDIETPYLTKSTPEGARDYLVPSRVYPGNFYALPQSPQILKQLLMTAGFDKYYQIVRCFRDEDLRGDRQPEFTQIDLE
TSFLTKEEIQAITEDMLVDVVKEAKNITIDKPFPRMTYKEAMDRFGSDKPDIRFGLELQNVSDVVKDVDFKVFQSAIENG
GEVKAINAKAAAANFSRKDLDALGVFVANYGAKGLAWLKVEAGELKGPIAKFFPEEKATELKASLQAEDGDLLLFAADKA
DIVAASLGALRNKLGKELNLINEEELAFLWVTDWPLFEYDEEAGRYVSAHHPFTLPKEEDIPLLETDSSKVMAEAYDIVL
NGYEIGGGSLRIYKKEVQESMFRALGFTDESAKEQFGFLMDALEYGTPPHGGIALGLDRIVMILAGRNNLRDTIAFPKTG
SAVDPLTNAPGEVSEAQLAELKLETVKKETN

Sequences:

>Translated_591_residues
MEKRTSYCGELNEAHIGQSVVLHGWVQKRRDLGGLIFIDLRDREGIVQVVFNPEFSKEALEIADSVRNEFVVTIKGTVHA
RGEKAINEKLATGKVEVLAEEITILNSSKTPPFYIEDGVNVSDELRLKYRYLDLRRPEMNNIFKMRHTVTRTFRNKLDAL
GFFDIETPYLTKSTPEGARDYLVPSRVYPGNFYALPQSPQILKQLLMTAGFDKYYQIVRCFRDEDLRGDRQPEFTQIDLE
TSFLTKEEIQAITEDMLVDVVKEAKNITIDKPFPRMTYKEAMDRFGSDKPDIRFGLELQNVSDVVKDVDFKVFQSAIENG
GEVKAINAKAAAANFSRKDLDALGVFVANYGAKGLAWLKVEAGELKGPIAKFFPEEKATELKASLQAEDGDLLLFAADKA
DIVAASLGALRNKLGKELNLINEEELAFLWVTDWPLFEYDEEAGRYVSAHHPFTLPKEEDIPLLETDSSKVMAEAYDIVL
NGYEIGGGSLRIYKKEVQESMFRALGFTDESAKEQFGFLMDALEYGTPPHGGIALGLDRIVMILAGRNNLRDTIAFPKTG
SAVDPLTNAPGEVSEAQLAELKLETVKKETN
>Mature_591_residues
MEKRTSYCGELNEAHIGQSVVLHGWVQKRRDLGGLIFIDLRDREGIVQVVFNPEFSKEALEIADSVRNEFVVTIKGTVHA
RGEKAINEKLATGKVEVLAEEITILNSSKTPPFYIEDGVNVSDELRLKYRYLDLRRPEMNNIFKMRHTVTRTFRNKLDAL
GFFDIETPYLTKSTPEGARDYLVPSRVYPGNFYALPQSPQILKQLLMTAGFDKYYQIVRCFRDEDLRGDRQPEFTQIDLE
TSFLTKEEIQAITEDMLVDVVKEAKNITIDKPFPRMTYKEAMDRFGSDKPDIRFGLELQNVSDVVKDVDFKVFQSAIENG
GEVKAINAKAAAANFSRKDLDALGVFVANYGAKGLAWLKVEAGELKGPIAKFFPEEKATELKASLQAEDGDLLLFAADKA
DIVAASLGALRNKLGKELNLINEEELAFLWVTDWPLFEYDEEAGRYVSAHHPFTLPKEEDIPLLETDSSKVMAEAYDIVL
NGYEIGGGSLRIYKKEVQESMFRALGFTDESAKEQFGFLMDALEYGTPPHGGIALGLDRIVMILAGRNNLRDTIAFPKTG
SAVDPLTNAPGEVSEAQLAELKLETVKKETN

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=594, Percent_Identity=40.5723905723906, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI45439306, Length=285, Percent_Identity=27.0175438596491, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI5031815, Length=247, Percent_Identity=27.9352226720648, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI194272210, Length=247, Percent_Identity=27.9352226720648, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4758762, Length=290, Percent_Identity=26.8965517241379, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788173, Length=594, Percent_Identity=52.3569023569024, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1789256, Length=289, Percent_Identity=25.6055363321799, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790571, Length=299, Percent_Identity=24.7491638795987, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=594, Percent_Identity=35.016835016835, Blast_Score=321, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI17551876, Length=256, Percent_Identity=30.078125, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71994340, Length=267, Percent_Identity=25.4681647940075, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=267, Percent_Identity=25.4681647940075, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=267, Percent_Identity=25.4681647940075, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=305, Percent_Identity=25.5737704918033, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=643, Percent_Identity=33.4370139968896, Blast_Score=295, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6323011, Length=265, Percent_Identity=30.9433962264151, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=293, Percent_Identity=24.9146757679181, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24584738, Length=611, Percent_Identity=37.4795417348609, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17136276, Length=248, Percent_Identity=30.241935483871, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921528, Length=284, Percent_Identity=28.5211267605634, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640849, Length=263, Percent_Identity=27.3764258555133, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640851, Length=244, Percent_Identity=27.4590163934426, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66329; Mature: 66329

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRTSYCGELNEAHIGQSVVLHGWVQKRRDLGGLIFIDLRDREGIVQVVFNPEFSKEAL
CCCCCCHHCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHH
EIADSVRNEFVVTIKGTVHARGEKAINEKLATGKVEVLAEEITILNSSKTPPFYIEDGVN
HHHHHHCCCEEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHEECCCCCCCEEEECCCC
VSDELRLKYRYLDLRRPEMNNIFKMRHTVTRTFRNKLDALGFFDIETPYLTKSTPEGARD
CCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCHH
YLVPSRVYPGNFYALPQSPQILKQLLMTAGFDKYYQIVRCFRDEDLRGDRQPEFTQIDLE
CCCCCEECCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECH
TSFLTKEEIQAITEDMLVDVVKEAKNITIDKPFPRMTYKEAMDRFGSDKPDIRFGLELQN
HHHCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEHH
VSDVVKDVDFKVFQSAIENGGEVKAINAKAAAANFSRKDLDALGVFVANYGAKGLAWLKV
HHHHHHHCCHHHHHHHHHCCCCEEEEECHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEE
EAGELKGPIAKFFPEEKATELKASLQAEDGDLLLFAADKADIVAASLGALRNKLGKELNL
ECCCCCCCHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHCC
INEEELAFLWVTDWPLFEYDEEAGRYVSAHHPFTLPKEEDIPLLETDSSKVMAEAYDIVL
CCCCCEEEEEEECCCCCCCCHHCCCEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHEE
NGYEIGGGSLRIYKKEVQESMFRALGFTDESAKEQFGFLMDALEYGTPPHGGIALGLDRI
ECEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECHHHE
VMILAGRNNLRDTIAFPKTGSAVDPLTNAPGEVSEAQLAELKLETVKKETN
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKRTSYCGELNEAHIGQSVVLHGWVQKRRDLGGLIFIDLRDREGIVQVVFNPEFSKEAL
CCCCCCHHCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCHHHH
EIADSVRNEFVVTIKGTVHARGEKAINEKLATGKVEVLAEEITILNSSKTPPFYIEDGVN
HHHHHHCCCEEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHEECCCCCCCEEEECCCC
VSDELRLKYRYLDLRRPEMNNIFKMRHTVTRTFRNKLDALGFFDIETPYLTKSTPEGARD
CCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCHH
YLVPSRVYPGNFYALPQSPQILKQLLMTAGFDKYYQIVRCFRDEDLRGDRQPEFTQIDLE
CCCCCEECCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECH
TSFLTKEEIQAITEDMLVDVVKEAKNITIDKPFPRMTYKEAMDRFGSDKPDIRFGLELQN
HHHCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEHH
VSDVVKDVDFKVFQSAIENGGEVKAINAKAAAANFSRKDLDALGVFVANYGAKGLAWLKV
HHHHHHHCCHHHHHHHHHCCCCEEEEECHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEE
EAGELKGPIAKFFPEEKATELKASLQAEDGDLLLFAADKADIVAASLGALRNKLGKELNL
ECCCCCCCHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHCC
INEEELAFLWVTDWPLFEYDEEAGRYVSAHHPFTLPKEEDIPLLETDSSKVMAEAYDIVL
CCCCCEEEEEEECCCCCCCCHHCCCEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHEE
NGYEIGGGSLRIYKKEVQESMFRALGFTDESAKEQFGFLMDALEYGTPPHGGIALGLDRI
ECEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECHHHE
VMILAGRNNLRDTIAFPKTGSAVDPLTNAPGEVSEAQLAELKLETVKKETN
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]