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The map label for this gene is greA

Identifier: 226224097

GI number: 226224097

Start: 1511124

End: 1511606

Strand: Reverse

Name: greA

Synonym: Lm4b_01506

Alternate gene names: 226224097

Gene position: 1511606-1511124 (Counterclockwise)

Preceding gene: 226224098

Following gene: 226224096

Centisome position: 51.9

GC content: 37.68

Gene sequence:

>483_bases
TTGGCGACAGAAAAAGTATTTCCAATGACCCTAGATGGGAAAGCAAAATTAGAAAATGAATTACAAGAATTAAAGACGGT
AAAACGTAAAGAAGTAGTAGAACGGATCAAAATTGCCCGTAGTTTTGGTGACTTATCAGAGAACTCCGAGTATGATTCCG
CAAAAGACGAACAAGCTTTTGTTGAAGGTCGTATCACTACTATTGAAAATATGATTCGTAACGCACAAATCATTGATGCT
GCTGAAGCACATAATGGTTTAGTAACACTTGGAAATACAGTTACTTTCATTGAACTACCAGACGGTGAAGAAGAAACTTA
CACAATCGTAGGTAGCGCTGAAGCAGACCCGTTTGAAGGTAAAATTTCCAATGATTCTCCAATTGCCAAAGGTTTACTTG
GTCATAAAGAAGGGGAAGAAGTAACTATCCAAACACCAGCTGGTGACATGAGCGTTAAAATCGAAAAAATCACTGCATCT
TAA

Upstream 100 bases:

>100_bases
TAATCTTTCTGACGAGTAGTAATATTTTTGATGAAGGTGCGGAGCGATATCTCCGTGCTTTTCTATACAATAATGAACAA
ACAAAAAGGAGCGAAATCGA

Downstream 100 bases:

>100_bases
TATTATCGGATGTTTTTATACAGCGGTTACTTATAAGGTGGTGAATGCAAATGGCAGACAAACGACAATCAAATAACCGT
CGAATTAAACAAAACTTGGT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MATEKVFPMTLDGKAKLENELQELKTVKRKEVVERIKIARSFGDLSENSEYDSAKDEQAFVEGRITTIENMIRNAQIIDA
AEAHNGLVTLGNTVTFIELPDGEEETYTIVGSAEADPFEGKISNDSPIAKGLLGHKEGEEVTIQTPAGDMSVKIEKITAS

Sequences:

>Translated_160_residues
MATEKVFPMTLDGKAKLENELQELKTVKRKEVVERIKIARSFGDLSENSEYDSAKDEQAFVEGRITTIENMIRNAQIIDA
AEAHNGLVTLGNTVTFIELPDGEEETYTIVGSAEADPFEGKISNDSPIAKGLLGHKEGEEVTIQTPAGDMSVKIEKITAS
>Mature_159_residues
ATEKVFPMTLDGKAKLENELQELKTVKRKEVVERIKIARSFGDLSENSEYDSAKDEQAFVEGRITTIENMIRNAQIIDAA
EAHNGLVTLGNTVTFIELPDGEEETYTIVGSAEADPFEGKISNDSPIAKGLLGHKEGEEVTIQTPAGDMSVKIEKITAS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=46.7532467532467, Blast_Score=134, Evalue=3e-33,
Organism=Escherichia coli, GI87082261, Length=149, Percent_Identity=33.5570469798658, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_LISIN (P64278)

Other databases:

- EMBL:   AL596168
- PIR:   AB1624
- RefSeq:   NP_470867.1
- ProteinModelPortal:   P64278
- SMR:   P64278
- GeneID:   1130145
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin1531
- GenoList:   LIN1531
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   LINN272626:LIN1531-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17485; Mature: 17354

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEKVFPMTLDGKAKLENELQELKTVKRKEVVERIKIARSFGDLSENSEYDSAKDEQAF
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VEGRITTIENMIRNAQIIDAAEAHNGLVTLGNTVTFIELPDGEEETYTIVGSAEADPFEG
HHCHHHHHHHHHHCCCEEEHHHHCCCEEEECCEEEEEECCCCCCCEEEEEECCCCCCCCC
KISNDSPIAKGLLGHKEGEEVTIQTPAGDMSVKIEKITAS
CCCCCCCHHHHHCCCCCCCEEEEECCCCCCEEEEEEEECC
>Mature Secondary Structure 
ATEKVFPMTLDGKAKLENELQELKTVKRKEVVERIKIARSFGDLSENSEYDSAKDEQAF
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VEGRITTIENMIRNAQIIDAAEAHNGLVTLGNTVTFIELPDGEEETYTIVGSAEADPFEG
HHCHHHHHHHHHHCCCEEEHHHHCCCEEEECCEEEEEECCCCCCCEEEEEECCCCCCCCC
KISNDSPIAKGLLGHKEGEEVTIQTPAGDMSVKIEKITAS
CCCCCCCHHHHHCCCCCCCEEEEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669