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The map label for this gene is rsmE [H]

Identifier: 226224071

GI number: 226224071

Start: 1485944

End: 1486711

Strand: Reverse

Name: rsmE [H]

Synonym: Lm4b_01480

Alternate gene names: 226224071

Gene position: 1486711-1485944 (Counterclockwise)

Preceding gene: 226224072

Following gene: 226224070

Centisome position: 51.04

GC content: 38.02

Gene sequence:

>768_bases
ATGCAACGTTATTTTGTAACAAATGAGCTAGAGAATGATAACGAAACACTATCCATTACTGGAGAAAACTTCCATCATAT
TACTCGTGTTATGCGAATGAAAGAATCCGACCAAGTTTTCATTGTTTTTCCTAGCAAAAAAACATGTATTGCCGCTATTA
CAGCTATAAACGATGATGAAGTATTTTTAAATTTAGTGAAATGGCTAGAAGAAGATACCGAATTGCCTATAGAAATCACC
ATCGCAAGTGGTCTACCAAAAGGCGACAAACTAGAACTCATCATTCAAAAAAGTACAGAACTAGGCGCACACAAATTTAT
CCCTTATAAAGCAGAACGATCTGTGGTGAAATGGGATGACAAGAAAGTCATCAAAAAAATCGAGCGACTTCAAAAAATTG
CGCAAGAAGCTGCTGAACAATCGCACCGGACAATCATTCCAGAAGTTCATTATGCTGCTAGTTTCAAAGAGTTAATGTTT
GACCATGTTCACTATGACTTTGTTATCGCAGCATATGAAGAGAGCGCCAGAGAAGGTGAGGACAGCGCGCTTGCTAACTT
ATTCCAAACCATGAAGCCTGGACAGTCTGTTTTATGTGTTTTTGGGCCTGAAGGAGGCATTAGCGAAAAAGAGCTAATGC
AACTTCAAGAAGTAAATGCAAAACTCGCAGGACTTGGCCCGAGAATATTAAGAACAGAAACCGCACCACTCTATTGTTTA
TCCGCTGCTTCTTTCTATTTTGATCTTTATAAAAACAAAAAAGAATAA

Upstream 100 bases:

>100_bases
AGTAGTCGAAGAAGCACTTAAAAAGGCTGGGCTTGTTATTGAAAAAATGGAGCAACAAGGGGACTGGGTTGCGATTATTT
CCAAACGAGGAGTGGAATAA

Downstream 100 bases:

>100_bases
CATTTCAAAGTTTTATGCGAAAGTTAACGATTTCATGTTGACGATGCCCGATGTTTCGTATATAATTTTTAATGAGTGAA
TGCTTATCTGCATTACTTCA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MQRYFVTNELENDNETLSITGENFHHITRVMRMKESDQVFIVFPSKKTCIAAITAINDDEVFLNLVKWLEEDTELPIEIT
IASGLPKGDKLELIIQKSTELGAHKFIPYKAERSVVKWDDKKVIKKIERLQKIAQEAAEQSHRTIIPEVHYAASFKELMF
DHVHYDFVIAAYEESAREGEDSALANLFQTMKPGQSVLCVFGPEGGISEKELMQLQEVNAKLAGLGPRILRTETAPLYCL
SAASFYFDLYKNKKE

Sequences:

>Translated_255_residues
MQRYFVTNELENDNETLSITGENFHHITRVMRMKESDQVFIVFPSKKTCIAAITAINDDEVFLNLVKWLEEDTELPIEIT
IASGLPKGDKLELIIQKSTELGAHKFIPYKAERSVVKWDDKKVIKKIERLQKIAQEAAEQSHRTIIPEVHYAASFKELMF
DHVHYDFVIAAYEESAREGEDSALANLFQTMKPGQSVLCVFGPEGGISEKELMQLQEVNAKLAGLGPRILRTETAPLYCL
SAASFYFDLYKNKKE
>Mature_255_residues
MQRYFVTNELENDNETLSITGENFHHITRVMRMKESDQVFIVFPSKKTCIAAITAINDDEVFLNLVKWLEEDTELPIEIT
IASGLPKGDKLELIIQKSTELGAHKFIPYKAERSVVKWDDKKVIKKIERLQKIAQEAAEQSHRTIIPEVHYAASFKELMF
DHVHYDFVIAAYEESAREGEDSALANLFQTMKPGQSVLCVFGPEGGISEKELMQLQEVNAKLAGLGPRILRTETAPLYCL
SAASFYFDLYKNKKE

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=224, Percent_Identity=30.8035714285714, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 29077; Mature: 29077

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYFVTNELENDNETLSITGENFHHITRVMRMKESDQVFIVFPSKKTCIAAITAINDDE
CCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCHH
VFLNLVKWLEEDTELPIEITIASGLPKGDKLELIIQKSTELGAHKFIPYKAERSVVKWDD
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCH
KKVIKKIERLQKIAQEAAEQSHRTIIPEVHYAASFKELMFDHVHYDFVIAAYEESAREGE
HHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHEEEEEEEHHHHCCCC
DSALANLFQTMKPGQSVLCVFGPEGGISEKELMQLQEVNAKLAGLGPRILRTETAPLYCL
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHEECCCCCEEEH
SAASFYFDLYKNKKE
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MQRYFVTNELENDNETLSITGENFHHITRVMRMKESDQVFIVFPSKKTCIAAITAINDDE
CCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCHH
VFLNLVKWLEEDTELPIEITIASGLPKGDKLELIIQKSTELGAHKFIPYKAERSVVKWDD
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCH
KKVIKKIERLQKIAQEAAEQSHRTIIPEVHYAASFKELMFDHVHYDFVIAAYEESAREGE
HHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHEEEEEEEHHHHCCCC
DSALANLFQTMKPGQSVLCVFGPEGGISEKELMQLQEVNAKLAGLGPRILRTETAPLYCL
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHEECCCCCEEEH
SAASFYFDLYKNKKE
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]