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The map label for this gene is dapG [H]

Identifier: 226224037

GI number: 226224037

Start: 1451913

End: 1453124

Strand: Reverse

Name: dapG [H]

Synonym: Lm4b_01446

Alternate gene names: 226224037

Gene position: 1453124-1451913 (Counterclockwise)

Preceding gene: 226224038

Following gene: 226224036

Centisome position: 49.89

GC content: 38.37

Gene sequence:

>1212_bases
ATGAAAATTATTGTCCAAAAATTTGGCGGAACATCCGTACAAAATGAAAAATCACGTTTGATGGCATTCAATCATATCAA
ACAAGTACTAAAAGAAGGATATAAAGTTGTTGTTGTCGTTTCAGCCATTGGTAGATACGGAGATCCTTATGCGACAGACA
CACTATTAGAACTTATTGGTGCTAAAAATACAAAGCTAACAGCAAGAGAACAAGATACTTTACTCTCTGTAGGCGAAACT
ATTTCAGCATCTGTATTTACCAATATGTTAAAAGAAGCAAATATTAAAGCAGAGGCGTTTTCTGGTGGTCAAGCAGGCAT
CATTACATCAGACGACCATTTAAATGCAAAAATCACAGAAGTGGATACAGCTCGTTTGAAAGATGCATTAACGAACCTTG
ATGTAGCTGTAGTTGCTGGCTTCCAAGGTATTACTGCAAATGGGGATATTTCCACATTAGGACGCGGAGGAAGTGATACA
TCCGCAGCGGCTCTAGGAGTTTCGTTACAAGCAGACTATATTGATATCTTTACAGATGTAGATGGAATGATGACAGCCGA
TCCACGTATTGTAGAACACGCACGTTCACTACCACGAGTAAGTTACAACGAAGTAAGCAATATGGCGTATCAAGGTGCAA
AAGTGATCCATCCACGTGCAGTTGAGATAGCGATGACTGCAAAAATACCAATGCGAATCCGTTCTACTTATTTGGAAAGC
ACTGGAACACTTGTTACTTCATTAACCAATGATTCAGATCATTTTGACGTGAAAGAACGAATAGTAACCGGTGTAGCACA
TGTAACCAATTTAACACAAATATCCGTACAAACAGATTCGGTAAAAGCGCAACAACTCGCTTTTAAAATATTAGCAGATG
CAGGAATCAGCCTTGATTTCATTAACATCTCTACCAACTCTGTCATTTTTACGGTGCCAGAAGAAAAATCTCATGTAGTA
AAACAACTATTAGAAGATGCAGACTTACAAACAACTGTACGACAAGCTTGTGCGAAAGTTTCTATTGTTGGCGCCGGAAT
CACAGGCGTTCCCGGTGTTACTGCAAAAATCGTTGGCGCATTATCTGAAAAAAATATCCCTATTTTACAATCAGCGGATA
GTCATACTACTATTTGGGTATTAGTAAAAGAAGAGGACTTAATTTCAGCAGTCAATGCCCTTCATGACGTATTTTGTTTG
GAAATTAAGTAA

Upstream 100 bases:

>100_bases
TGTGGATTGTTTCTGACAACTTACTAAAAGGTGCCGCATGGAATTCGATTCAAATAGCTGAAAGTTTAGTTAAACTAGCG
ATTATTTAGAGGTGTAAGTA

Downstream 100 bases:

>100_bases
GGAGGAAATGAAAGATGGATTTAGGGAAAGTAATTACAGCAATGGTGACACCAATTCACCCAGAGAAAGATAAAGTATGC
AAAAAAAGAATTCATCATCT

Product: aspartate kinase I

Products: NA

Alternate protein names: Aspartate kinase 1; Aspartokinase I [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKIIVQKFGGTSVQNEKSRLMAFNHIKQVLKEGYKVVVVVSAIGRYGDPYATDTLLELIGAKNTKLTAREQDTLLSVGET
ISASVFTNMLKEANIKAEAFSGGQAGIITSDDHLNAKITEVDTARLKDALTNLDVAVVAGFQGITANGDISTLGRGGSDT
SAAALGVSLQADYIDIFTDVDGMMTADPRIVEHARSLPRVSYNEVSNMAYQGAKVIHPRAVEIAMTAKIPMRIRSTYLES
TGTLVTSLTNDSDHFDVKERIVTGVAHVTNLTQISVQTDSVKAQQLAFKILADAGISLDFINISTNSVIFTVPEEKSHVV
KQLLEDADLQTTVRQACAKVSIVGAGITGVPGVTAKIVGALSEKNIPILQSADSHTTIWVLVKEEDLISAVNALHDVFCL
EIK

Sequences:

>Translated_403_residues
MKIIVQKFGGTSVQNEKSRLMAFNHIKQVLKEGYKVVVVVSAIGRYGDPYATDTLLELIGAKNTKLTAREQDTLLSVGET
ISASVFTNMLKEANIKAEAFSGGQAGIITSDDHLNAKITEVDTARLKDALTNLDVAVVAGFQGITANGDISTLGRGGSDT
SAAALGVSLQADYIDIFTDVDGMMTADPRIVEHARSLPRVSYNEVSNMAYQGAKVIHPRAVEIAMTAKIPMRIRSTYLES
TGTLVTSLTNDSDHFDVKERIVTGVAHVTNLTQISVQTDSVKAQQLAFKILADAGISLDFINISTNSVIFTVPEEKSHVV
KQLLEDADLQTTVRQACAKVSIVGAGITGVPGVTAKIVGALSEKNIPILQSADSHTTIWVLVKEEDLISAVNALHDVFCL
EIK
>Mature_403_residues
MKIIVQKFGGTSVQNEKSRLMAFNHIKQVLKEGYKVVVVVSAIGRYGDPYATDTLLELIGAKNTKLTAREQDTLLSVGET
ISASVFTNMLKEANIKAEAFSGGQAGIITSDDHLNAKITEVDTARLKDALTNLDVAVVAGFQGITANGDISTLGRGGSDT
SAAALGVSLQADYIDIFTDVDGMMTADPRIVEHARSLPRVSYNEVSNMAYQGAKVIHPRAVEIAMTAKIPMRIRSTYLES
TGTLVTSLTNDSDHFDVKERIVTGVAHVTNLTQISVQTDSVKAQQLAFKILADAGISLDFINISTNSVIFTVPEEKSHVV
KQLLEDADLQTTVRQACAKVSIVGAGITGVPGVTAKIVGALSEKNIPILQSADSHTTIWVLVKEEDLISAVNALHDVFCL
EIK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=280, Percent_Identity=32.8571428571429, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790455, Length=347, Percent_Identity=29.3948126801153, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790376, Length=264, Percent_Identity=26.1363636363636, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=377, Percent_Identity=25.7294429708223, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43141; Mature: 43141

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVQKFGGTSVQNEKSRLMAFNHIKQVLKEGYKVVVVVSAIGRYGDPYATDTLLELIG
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCHHHHHHHHHC
AKNTKLTAREQDTLLSVGETISASVFTNMLKEANIKAEAFSGGQAGIITSDDHLNAKITE
CCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCEEEEE
VDTARLKDALTNLDVAVVAGFQGITANGDISTLGRGGSDTSAAALGVSLQADYIDIFTDV
CHHHHHHHHHHCCCEEEEECCCCEECCCCHHCCCCCCCCCCHHEEEEEEECCEEEEEECC
DGMMTADPRIVEHARSLPRVSYNEVSNMAYQGAKVIHPRAVEIAMTAKIPMRIRSTYLES
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCEEEEEEEECCCHHHHHHHHHC
TGTLVTSLTNDSDHFDVKERIVTGVAHVTNLTQISVQTDSVKAQQLAFKILADAGISLDF
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEE
INISTNSVIFTVPEEKSHVVKQLLEDADLQTTVRQACAKVSIVGAGITGVPGVTAKIVGA
EEECCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHH
LSEKNIPILQSADSHTTIWVLVKEEDLISAVNALHDVFCLEIK
HCCCCCCEEECCCCCEEEEEEEECHHHHHHHHHHHHEEEEEEC
>Mature Secondary Structure
MKIIVQKFGGTSVQNEKSRLMAFNHIKQVLKEGYKVVVVVSAIGRYGDPYATDTLLELIG
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCCHHHHHHHHHC
AKNTKLTAREQDTLLSVGETISASVFTNMLKEANIKAEAFSGGQAGIITSDDHLNAKITE
CCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCEEEEE
VDTARLKDALTNLDVAVVAGFQGITANGDISTLGRGGSDTSAAALGVSLQADYIDIFTDV
CHHHHHHHHHHCCCEEEEECCCCEECCCCHHCCCCCCCCCCHHEEEEEEECCEEEEEECC
DGMMTADPRIVEHARSLPRVSYNEVSNMAYQGAKVIHPRAVEIAMTAKIPMRIRSTYLES
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCEEEEEEEECCCHHHHHHHHHC
TGTLVTSLTNDSDHFDVKERIVTGVAHVTNLTQISVQTDSVKAQQLAFKILADAGISLDF
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEE
INISTNSVIFTVPEEKSHVVKQLLEDADLQTTVRQACAKVSIVGAGITGVPGVTAKIVGA
EEECCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHH
LSEKNIPILQSADSHTTIWVLVKEEDLISAVNALHDVFCLEIK
HCCCCCCEEECCCCCEEEEEEEECHHHHHHHHHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377 [H]