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The map label for this gene is truB

Identifier: 226223929

GI number: 226223929

Start: 1340458

End: 1341372

Strand: Direct

Name: truB

Synonym: Lm4b_01336

Alternate gene names: 226223929

Gene position: 1340458-1341372 (Clockwise)

Preceding gene: 226223928

Following gene: 226223930

Centisome position: 46.02

GC content: 40.11

Gene sequence:

>915_bases
ATGAACGGCATTATCCCACTGTGGAAAGAACGCGGGATGACAAGTCATGATTGCGTTTTTAAATTAAGAAAGATTTTACA
TACGAAAAAAGTTGGACACACTGGCACGCTGGATCCAGAAGTAGAGGGTGTACTTCCAATTTGTATCGGCCGTGCGACGA
AACTAGCAGAATACGTAACAGATGAAGGAAAAGTCTACGTAGCAGAAATAACTTTAGGCAAATCGACTACAACGGAAGAT
GCGACTGGTGAAACGGTTATGACCAAGGAATTAGCAGACATTTCCGCTGATGAACTTCAAGCCGCGCTCACTAAATTGAC
TGGAAAAATTACTCAAATTCCCCCTATGTTTTCTGCTGTAAAAGTCAATGGCAAGAAATTGTATGAATATGCAAGAGCTG
GTATAGAGGTGGAACGCCCATCCAGGCAAGTGGATATTTATTCGTTAACTCGCTTAGATGGCGAGGCTACGCTAAATGAA
GCGAATCCGACTTTTCAGTTAGAAATATCTTGTGGAAAAGGTACTTATATTCGCACACTTGCAGTTATGATTGGTGAATT
ATTAGGATATCCAGCGCACATGTCCAAACTAGAACGGACTCGTAGCGGCTTTTTCAAAAAAGAAGATTGCTTAACGCTGG
CAGAAATTGACGAAATGATGCAAGCGAGTGATAGCAGCTTTTTATATCCACTTGAAAAAGGCATTGAATCCATGGCCAAA
TTAGTGATTGATGAAGAAATCCATGCGAAAGTTTTAAATGGCGGCCTTTTACCAAAATCATTATTTATAGAGGTAGAGAA
TGAGCCTCGTGCCGCGCTTATTTTCAAAGATAAATTAACAGCAATTTACAAACCTCATCCAGAGAAAAATGAACTTTGGA
AACCAGAAAAAGTCATCGAGTTACATCAAGCATAG

Upstream 100 bases:

>100_bases
AATAATAAATAAAAGTTTAAGGCAGTCCCTTTATCTTCTCATTCAAACGGGATATAAGTGGGAATTGTCTTAAACTTTTT
TTCTTAAAAGGAGGAGCTTG

Downstream 100 bases:

>100_bases
TTATTGCGACTATAGCTTTCTTATATTATGATATGAGAGGAATTCGAATTTAGAGGAGAAGTGGCAAAAATGAAGACGAT
TTACTTACATCATCCGATTA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVTDEGKVYVAEITLGKSTTTED
ATGETVMTKELADISADELQAALTKLTGKITQIPPMFSAVKVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGEATLNE
ANPTFQLEISCGKGTYIRTLAVMIGELLGYPAHMSKLERTRSGFFKKEDCLTLAEIDEMMQASDSSFLYPLEKGIESMAK
LVIDEEIHAKVLNGGLLPKSLFIEVENEPRAALIFKDKLTAIYKPHPEKNELWKPEKVIELHQA

Sequences:

>Translated_304_residues
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVTDEGKVYVAEITLGKSTTTED
ATGETVMTKELADISADELQAALTKLTGKITQIPPMFSAVKVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGEATLNE
ANPTFQLEISCGKGTYIRTLAVMIGELLGYPAHMSKLERTRSGFFKKEDCLTLAEIDEMMQASDSSFLYPLEKGIESMAK
LVIDEEIHAKVLNGGLLPKSLFIEVENEPRAALIFKDKLTAIYKPHPEKNELWKPEKVIELHQA
>Mature_304_residues
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVTDEGKVYVAEITLGKSTTTED
ATGETVMTKELADISADELQAALTKLTGKITQIPPMFSAVKVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGEATLNE
ANPTFQLEISCGKGTYIRTLAVMIGELLGYPAHMSKLERTRSGFFKKEDCLTLAEIDEMMQASDSSFLYPLEKGIESMAK
LVIDEEIHAKVLNGGLLPKSLFIEVENEPRAALIFKDKLTAIYKPHPEKNELWKPEKVIELHQA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=255, Percent_Identity=32.5490196078431, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI215599015, Length=282, Percent_Identity=32.6241134751773, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4503337, Length=282, Percent_Identity=32.6241134751773, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI2367200, Length=224, Percent_Identity=42.8571428571429, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17553978, Length=282, Percent_Identity=28.7234042553192, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=285, Percent_Identity=30.8771929824561, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324037, Length=132, Percent_Identity=43.9393939393939, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281364189, Length=241, Percent_Identity=33.195020746888, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281364187, Length=241, Percent_Identity=33.195020746888, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281364185, Length=241, Percent_Identity=33.195020746888, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281364183, Length=241, Percent_Identity=33.195020746888, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI62471759, Length=241, Percent_Identity=33.195020746888, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17975520, Length=241, Percent_Identity=33.195020746888, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33840; Mature: 33840

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVT
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DEGKVYVAEITLGKSTTTEDATGETVMTKELADISADELQAALTKLTGKITQIPPMFSAV
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHCCCCCHHHE
KVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGEATLNEANPTFQLEISCGKGTYIRTL
EECHHHHHHHHHCCCEEECCCCEEEEEEEEECCCCCEECCCCCEEEEEEECCCCHHHHHH
AVMIGELLGYPAHMSKLERTRSGFFKKEDCLTLAEIDEMMQASDSSFLYPLEKGIESMAK
HHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHH
LVIDEEIHAKVLNGGLLPKSLFIEVENEPRAALIFKDKLTAIYKPHPEKNELWKPEKVIE
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCHHEEECCCCCCCCCCCCHHHHH
LHQA
CCCC
>Mature Secondary Structure
MNGIIPLWKERGMTSHDCVFKLRKILHTKKVGHTGTLDPEVEGVLPICIGRATKLAEYVT
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DEGKVYVAEITLGKSTTTEDATGETVMTKELADISADELQAALTKLTGKITQIPPMFSAV
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHCCCCCHHHE
KVNGKKLYEYARAGIEVERPSRQVDIYSLTRLDGEATLNEANPTFQLEISCGKGTYIRTL
EECHHHHHHHHHCCCEEECCCCEEEEEEEEECCCCCEECCCCCEEEEEEECCCCHHHHHH
AVMIGELLGYPAHMSKLERTRSGFFKKEDCLTLAEIDEMMQASDSSFLYPLEKGIESMAK
HHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHH
LVIDEEIHAKVLNGGLLPKSLFIEVENEPRAALIFKDKLTAIYKPHPEKNELWKPEKVIE
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCHHEEECCCCCCCCCCCCHHHHH
LHQA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]