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The map label for this gene is rbfA

Identifier: 226223928

GI number: 226223928

Start: 1340018

End: 1340362

Strand: Direct

Name: rbfA

Synonym: Lm4b_01335

Alternate gene names: 226223928

Gene position: 1340018-1340362 (Clockwise)

Preceding gene: 226223927

Following gene: 226223929

Centisome position: 46.01

GC content: 38.26

Gene sequence:

>345_bases
TTGAACGTTCGAGCAAATCGTGTCAGTGAGCAAATGAAAAAAGAATTGGGCGATATTTTAAATCGTAAAATTAAAGACCC
GCGCTTAGGTTTTGTTACTGTAACAGGAGTTGATGTTACTGGTGATTTACAAGAAGCTAAGGTGTTCATTTCCATTCTTG
GTACAGATAAGGAAAAAGAAAATACGTTACTCGCACTTGCGAAAGCGCATGGCTTTATCCGCTCTGAAATCGGTCGCCGT
ATTCGACTTCGTAAAGTTCCAGAAATGTCTTTTGAAATAGATAATTCCATCGCTTACGGAAATCGAATCGATGAATTGCT
TCGCGACTTAAATAACGATCAATAA

Upstream 100 bases:

>100_bases
GAAAAAGAAGCCAGAGAAGTACTTGCTTTTCTTGATTCTTTTCCTGAGTGGGAACGCGCCGAGACAGTTATGGAGAAGTT
ATAAAAAGAGGTGAATACAC

Downstream 100 bases:

>100_bases
TAAATAAAAGTTTAAGGCAGTCCCTTTATCTTCTCATTCAAACGGGATATAAGTGGGAATTGTCTTAAACTTTTTTTCTT
AAAAGGAGGAGCTTGATGAA

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKENTLLALAKAHGFIRSEIGRR
IRLRKVPEMSFEIDNSIAYGNRIDELLRDLNNDQ

Sequences:

>Translated_114_residues
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKENTLLALAKAHGFIRSEIGRR
IRLRKVPEMSFEIDNSIAYGNRIDELLRDLNNDQ
>Mature_114_residues
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKENTLLALAKAHGFIRSEIGRR
IRLRKVPEMSFEIDNSIAYGNRIDELLRDLNNDQ

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA [H]

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family [H]

Homologues:

Organism=Escherichia coli, GI1789558, Length=108, Percent_Identity=46.2962962962963, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR000238
- InterPro:   IPR020053
- ProDom:   PD007327 [H]

Pfam domain/function: PF02033 RBFA [H]

EC number: NA

Molecular weight: Translated: 12968; Mature: 12968

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHEECCCCCCC
NTLLALAKAHGFIRSEIGRRIRLRKVPEMSFEIDNSIAYGNRIDELLRDLNNDQ
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVRANRVSEQMKKELGDILNRKIKDPRLGFVTVTGVDVTGDLQEAKVFISILGTDKEKE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHEECCCCCCC
NTLLALAKAHGFIRSEIGRRIRLRKVPEMSFEIDNSIAYGNRIDELLRDLNNDQ
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]