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The map label for this gene is infB

Identifier: 226223926

GI number: 226223926

Start: 1337378

End: 1339726

Strand: Direct

Name: infB

Synonym: Lm4b_01333

Alternate gene names: 226223926

Gene position: 1337378-1339726 (Clockwise)

Preceding gene: 226223925

Following gene: 226223927

Centisome position: 45.92

GC content: 39.63

Gene sequence:

>2349_bases
ATGAGTAAAGTTCGTGTATATGAATACGCAAAAGAACATCAAGTATCAAGCAAAAAAGTCATTGAAGCATTAAAAGACTT
AGGTATTGAAGTGGCAAATCACATGTCCACGATTAATGAAAATGCATTAAGACAATTAGATAATGCCATTGATGGCACAA
ATAAAAAAGCCGAAGCACCAAAGAAAGAAACTACTAGCAACGAAAATGGAAATAGTAAGGGGCCAAACAAACCAAATATG
ACAAATAGTAATGAAAAGTCGAATAAACCAAATAACCCAGCAGGGCAAGCTAATAAACCAGCAACTGCAAACAAAAGCCA
AGGTGCAAAACCAGCGACAAACAAACCAGCAAACACAAGTAAACAAACACAATCAAGTGGTAACCAACAACAAGCTGGCG
GACAAAAACGCAACAACAATAACAACAGCAATCGTCCAGGCGGCGGCAATCCAAACCGTCCAGGCGGTAACAATCGCCCG
AATCGTGGCGGCAATTTTAATAATAAAGGTCGTAACACGAAGAAGAAAGGGAAGCTAAACCACAGTACAGTACCGCCAAC
TCCGCCAAAACCAAAAGAACTTCCTGAAAAAATTGTGTTCAGCGAATCTCTAACAGTAGCGGAATTAGCGAAAAAATTAT
ACAGAGAACCATCTGAACTAATCAAAAAACTATTTATGCTTGGTGTTGTTGCAACGATTAACCAATCATTAGATAAAGAT
GCAATCGAACTAATTTGTGACGACTACGGTGTACAAGTAGAAGAAGAAATTAAAGTCGATGTAACAGATTTAGATGTGTA
CTTTGAAAATGAACTAAATGAAGCGGTTGACGAGTCTAAACTTGTTGAACGCCCACCAGTTGTTACTATTATGGGACACG
TTGACCATGGTAAAACAACATTACTAGATTCCCTTCGTAATACAAAAGTTACTTTAGGAGAAGCTGGTGGTATCACCCAA
CATATCGGTGCTTACCAACTTGAAATCCATGACAAAAAAATCACTTTCCTTGATACACCGGGACATGCTGCCTTTACTGC
AATGCGTGCTCGTGGTGCGCAAATCACGGATATTACGATTTTAGTTGTAGCAGCAGATGATGGTGTTATGCCACAAACAA
TTGAAGCAATCAACCACGCAAAAGCTGCGGGAATGCCAATTATTGTTGCTGTCAACAAAATTGATAAACCACAAGCAAAC
CCTGACCGCGTAATGCAAGAACTAACAGAATATGAATTAGTTCCTGAAGCATGGGGTGGCGATACAATTTTCGCGCCAAT
CTCTGCTAAATTCGGTGAAGGTCTTGAAAACTTGTTAGATATGATTTTACTTGTTTCTGAAGTAGAAGAATTAAAAGCAA
ATCCAGATCGTCGTGCTATCGGTTCTGTTATCGAAGCAGAACTTGATAAAGGCCGCGGTCCAGTTGCGACTCTACTAGTA
CAAGATGGAACGCTTAATATTGGAGACCCAATTGTTGTTGGTAACACGTTTGGTCGTGTTCGTGCAATGGTCAATGATTT
AGGCCGTCGCGTGAAAAAAGTTGGTCCAAGTACACCAGTTGAAATCACTGGATTAAATGATGTACCACAAGCCGGCGATC
GCTTTGTTGTTTTTGAAGATGAAAAAACAGCAAGAAACATCGGTGAAACTCGTGCAAGCCGTGCGTTAGTAGCGCAACGT
TCTGCAACCAACCGCGTAAGTTTAGACAACTTATTTGAACATATGAAAGCTGGCGAAATGAAAGAAGTTAACGTTATTAT
TAAAGCAGACGTTCAAGGTTCTGTAGAGGCTCTTGCTGCATCTCTTCGCAAAATTGATGTAGAGGGTGTTAACGTTAAAA
TTATTCATACTGCTGTTGGTGCTATCAATGAATCAGATATCACACTTGCTGCAGCTTCTAATGCAATTGTTATTGGATTT
AATGTTCGTCCAACCGCTCAAGCTCGTGAAGCAGCAGAAAACGAAAGTGTAGACATTCGTTTACACCGTGTTATCTATAA
AGCCATTGATGAAATTGAAGCAGCGATGAAAGGGATGCTTGATCCAGAATTCCAAGAAAAAATTATTGGTCAAGCGCAAG
TTCGTCAAACAATCAATGTTTCCAAAGTGGGTACAATTGCCGGCTGTTACGTAACAGATGGTAAAATTACTCGTGATAGT
GGCGTTCGTATTATCCGTGACGGAATTGTTGTCTTTGAAGGCGAAATTGCTACACTTAAACGCTTTAAAGATGATGCGAA
AGAAGTTGCAAAAGGATACGAATGTGGTATCACAGTACAAAACTTCAACGATATCAAAGAAGATGATGTCATTGAAGCGT
ACGTTATGGAAGAAATTGAAAGAAAATGA

Upstream 100 bases:

>100_bases
ATCGGCAAAGACACACGTGCAATAGTTGCCATACTTGATAAAGGATTTGCTGTTAAATTAGCAGAATTACTCGGTTGAAT
CTTATACGGAGGTGTACGAC

Downstream 100 bases:

>100_bases
TTCAATCAGTCGTTAGTGAATTTTTCATGCAGGAACCGCAAAACCTCAAAGAAAAACGAGCTATCCTGAAACGAATAGTA
ACGAGAGCAAAACAAAAATT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 782; Mature: 781

Protein sequence:

>782_residues
MSKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAIDGTNKKAEAPKKETTSNENGNSKGPNKPNM
TNSNEKSNKPNNPAGQANKPATANKSQGAKPATNKPANTSKQTQSSGNQQQAGGQKRNNNNNSNRPGGGNPNRPGGNNRP
NRGGNFNNKGRNTKKKGKLNHSTVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKD
AIELICDDYGVQVEEEIKVDVTDLDVYFENELNEAVDESKLVERPPVVTIMGHVDHGKTTLLDSLRNTKVTLGEAGGITQ
HIGAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITILVVAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQAN
PDRVMQELTEYELVPEAWGGDTIFAPISAKFGEGLENLLDMILLVSEVEELKANPDRRAIGSVIEAELDKGRGPVATLLV
QDGTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFEDEKTARNIGETRASRALVAQR
SATNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAASLRKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGF
NVRPTAQAREAAENESVDIRLHRVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDS
GVRIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIERK

Sequences:

>Translated_782_residues
MSKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAIDGTNKKAEAPKKETTSNENGNSKGPNKPNM
TNSNEKSNKPNNPAGQANKPATANKSQGAKPATNKPANTSKQTQSSGNQQQAGGQKRNNNNNSNRPGGGNPNRPGGNNRP
NRGGNFNNKGRNTKKKGKLNHSTVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKD
AIELICDDYGVQVEEEIKVDVTDLDVYFENELNEAVDESKLVERPPVVTIMGHVDHGKTTLLDSLRNTKVTLGEAGGITQ
HIGAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITILVVAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQAN
PDRVMQELTEYELVPEAWGGDTIFAPISAKFGEGLENLLDMILLVSEVEELKANPDRRAIGSVIEAELDKGRGPVATLLV
QDGTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFEDEKTARNIGETRASRALVAQR
SATNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAASLRKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGF
NVRPTAQAREAAENESVDIRLHRVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDS
GVRIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIERK
>Mature_781_residues
SKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAIDGTNKKAEAPKKETTSNENGNSKGPNKPNMT
NSNEKSNKPNNPAGQANKPATANKSQGAKPATNKPANTSKQTQSSGNQQQAGGQKRNNNNNSNRPGGGNPNRPGGNNRPN
RGGNFNNKGRNTKKKGKLNHSTVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKDA
IELICDDYGVQVEEEIKVDVTDLDVYFENELNEAVDESKLVERPPVVTIMGHVDHGKTTLLDSLRNTKVTLGEAGGITQH
IGAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITILVVAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQANP
DRVMQELTEYELVPEAWGGDTIFAPISAKFGEGLENLLDMILLVSEVEELKANPDRRAIGSVIEAELDKGRGPVATLLVQ
DGTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFEDEKTARNIGETRASRALVAQRS
ATNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAASLRKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGFN
VRPTAQAREAAENESVDIRLHRVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDSG
VRIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIERK

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=549, Percent_Identity=38.9799635701275, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI53729337, Length=549, Percent_Identity=38.9799635701275, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI84043963, Length=472, Percent_Identity=28.1779661016949, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI157426893, Length=252, Percent_Identity=30.1587301587302, Blast_Score=87, Evalue=7e-17,
Organism=Escherichia coli, GI1789559, Length=667, Percent_Identity=50.2248875562219, Blast_Score=626, Evalue=1e-180,
Organism=Escherichia coli, GI48994988, Length=146, Percent_Identity=35.6164383561644, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI2367247, Length=225, Percent_Identity=31.1111111111111, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788922, Length=232, Percent_Identity=28.8793103448276, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790412, Length=125, Percent_Identity=37.6, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1789737, Length=125, Percent_Identity=37.6, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=536, Percent_Identity=36.5671641791045, Blast_Score=310, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI212656558, Length=569, Percent_Identity=25.4833040421793, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=216, Percent_Identity=31.0185185185185, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=248, Percent_Identity=32.6612903225806, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324550, Length=623, Percent_Identity=38.6837881219904, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319282, Length=537, Percent_Identity=25.512104283054, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=256, Percent_Identity=32.421875, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=26.4462809917355, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28572034, Length=528, Percent_Identity=39.3939393939394, Blast_Score=336, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24656849, Length=470, Percent_Identity=28.0851063829787, Blast_Score=127, Evalue=4e-29,
Organism=Drosophila melanogaster, GI160714833, Length=73, Percent_Identity=72.6027397260274, Blast_Score=115, Evalue=2e-25,
Organism=Drosophila melanogaster, GI78706572, Length=224, Percent_Identity=29.9107142857143, Blast_Score=87, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281363316, Length=269, Percent_Identity=31.5985130111524, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17864358, Length=269, Percent_Identity=31.5985130111524, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24582462, Length=154, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 84956; Mature: 84825

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAIDGTNKKAEAP
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KKETTSNENGNSKGPNKPNMTNSNEKSNKPNNPAGQANKPATANKSQGAKPATNKPANTS
CHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KQTQSSGNQQQAGGQKRNNNNNSNRPGGGNPNRPGGNNRPNRGGNFNNKGRNTKKKGKLN
HHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HSTVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKD
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
AIELICDDYGVQVEEEIKVDVTDLDVYFENELNEAVDESKLVERPPVVTIMGHVDHGKTT
HHHHHHHCCCCEEECCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
LLDSLRNTKVTLGEAGGITQHIGAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITI
HHHHHCCCEEEEECCCCCHHHCCEEEEEEECCEEEEEECCCCHHHHHHHHCCCEEEEEEE
LVVAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQANPDRVMQELTEYELVPEAWGG
EEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
DTIFAPISAKFGEGLENLLDMILLVSEVEELKANPDRRAIGSVIEAELDKGRGPVATLLV
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEE
QDGTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFED
ECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECC
EKTARNIGETRASRALVAQRSATNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAA
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHH
SLRKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGFNVRPTAQAREAAENESVDIR
HHHHEECCCCEEEEEEEECCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHCCCCCEEHH
LHRVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCHHHCCCEEEEEEECCEEECCC
GVRIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIE
CCEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
RK
CC
>Mature Secondary Structure 
SKVRVYEYAKEHQVSSKKVIEALKDLGIEVANHMSTINENALRQLDNAIDGTNKKAEAP
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KKETTSNENGNSKGPNKPNMTNSNEKSNKPNNPAGQANKPATANKSQGAKPATNKPANTS
CHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KQTQSSGNQQQAGGQKRNNNNNSNRPGGGNPNRPGGNNRPNRGGNFNNKGRNTKKKGKLN
HHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HSTVPPTPPKPKELPEKIVFSESLTVAELAKKLYREPSELIKKLFMLGVVATINQSLDKD
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
AIELICDDYGVQVEEEIKVDVTDLDVYFENELNEAVDESKLVERPPVVTIMGHVDHGKTT
HHHHHHHCCCCEEECCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHH
LLDSLRNTKVTLGEAGGITQHIGAYQLEIHDKKITFLDTPGHAAFTAMRARGAQITDITI
HHHHHCCCEEEEECCCCCHHHCCEEEEEEECCEEEEEECCCCHHHHHHHHCCCEEEEEEE
LVVAADDGVMPQTIEAINHAKAAGMPIIVAVNKIDKPQANPDRVMQELTEYELVPEAWGG
EEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
DTIFAPISAKFGEGLENLLDMILLVSEVEELKANPDRRAIGSVIEAELDKGRGPVATLLV
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEE
QDGTLNIGDPIVVGNTFGRVRAMVNDLGRRVKKVGPSTPVEITGLNDVPQAGDRFVVFED
ECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECC
EKTARNIGETRASRALVAQRSATNRVSLDNLFEHMKAGEMKEVNVIIKADVQGSVEALAA
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHH
SLRKIDVEGVNVKIIHTAVGAINESDITLAAASNAIVIGFNVRPTAQAREAAENESVDIR
HHHHEECCCCEEEEEEEECCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHCCCCCEEHH
LHRVIYKAIDEIEAAMKGMLDPEFQEKIIGQAQVRQTINVSKVGTIAGCYVTDGKITRDS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCHHHCCCEEEEEEECCEEECCC
GVRIIRDGIVVFEGEIATLKRFKDDAKEVAKGYECGITVQNFNDIKEDDVIEAYVMEEIE
CCEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
RK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]