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The map label for this gene is ydfA [H]

Identifier: 226223902

GI number: 226223902

Start: 1312903

End: 1314198

Strand: Direct

Name: ydfA [H]

Synonym: Lm4b_01309

Alternate gene names: 226223902

Gene position: 1312903-1314198 (Clockwise)

Preceding gene: 226223901

Following gene: 226223905

Centisome position: 45.08

GC content: 36.65

Gene sequence:

>1296_bases
ATGCAGATTACTTTAGCTATTTTAGTATTTGCCTTCACATTATTTTTTGTGATTTGGCAACCAAGAAAATTATCAATCGG
ATGGTCAGCTTGCGGAGGTGCGCTAATTGCCATATTACTTGGCGTCGTCACTTTATCAGATGTCGCAACAGTTACTGGAA
TTGTTTGGAATGCGACGCTCGCTTTTATTGCTATTATAATTATTTCATTGATTTTAGATAAAATTGGCTTTTTTGAGTGG
GCGGCTTTACATATGGCGCGATTAGCAAGAGGTAATGGGTTATTAATGTTTATTCTTATTTCTATTTTGGGAGCTTTCGT
TGCTGCGTTGTTTGCTAATGACGGGGCGGCGCTAATTTTGACACCAATTGTTCTAGCGATGGTGCGAGCGCTGAATTTTG
ATGAGAAAAAGGTATTTCCATTTATTATTGCTAGCGGTTTTATAGCAGATACGACGTCCTTGCCGCTCGTAGTTAGTAAT
TTAGTCAATATAGTTTCGGCTGATTTTTTCGGTATTAGTTTTTCGCAGTATGCGCTTATTATGTGGATTCCAACGCTTTT
CTCTTTAGTTGCGAGTATTTTAATCTTATATTTTTATTTTCGGAAAGCATTACCTAAAAAATATGATATGCGAGTGATTC
GAGAGCCAGCTTCTGCAATTAAAGATAAAAAGATGTTTCGTGTTTCTTGGGGAATACTTATCATCCTTGTAGCAGGCTAT
TTTTTGAGTAGTTTTTTAGATATTCCAGTGTCATTTATTGCCTTGCTAGTTGCTGTTATCTTTTTGTTAGTCAGTGCAAA
AAGTCATGCGGTCTCGACAAAAGCTATTTTAAAAGGAGCACCTTGGAATATTGTGTTTTTCTCGGTTGGTATGTATGTAG
TTGTATATGGTTTGCAAAATGTCGGTATCACAAAATTATTATCAGAAGCAATTACATATGTTGCGCACTTTGGTTTATTT
GCGGGAACAGTTGGAATGGGATTTATTGCTGCAATACTGTCTTCTGTCATGAATAATTTGCCAACAGTAATGATAAATGC
ACTTGCAATCGATCATACGTCATTTACAGGCGTGATGAAAGAAGCGTTAGTCTATGCAAATGTCATTGGTTCTGATTTAG
GTCCGAAAATAACACCAATTGGCTCGCTAGCAACTTTACTTTGGCTACATGTTCTAGCGCAGAAAGGGATAAAGATAAGC
TGGGGCTCTTATTTCAAAATTGGCATTGTGATTACTATTCCAGTTTTATTCATAACGCTCGTCGGACTATATTTTTCACT
GCTTATTTGGAATTAA

Upstream 100 bases:

>100_bases
TTGGAAAATCGGTTTCAAATGTTTGATGTTTGGCAAATAGAGGCATATATTTAAAAATGTGCTAGTGAAAGGGAGGTTTT
CATTAGAGAAAGGGGAAGAA

Downstream 100 bases:

>100_bases
AACCCCCTGTACCAGAATGTACAGGGGGTTTTATTATGCCTTCTTATAAAGAACATGGGGCTTTAGTGGATCGCCATTTA
CTAAAGCGGGATGTTCAAAA

Product: arsenic efflux pump protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQITLAILVFAFTLFFVIWQPRKLSIGWSACGGALIAILLGVVTLSDVATVTGIVWNATLAFIAIIIISLILDKIGFFEW
AALHMARLARGNGLLMFILISILGAFVAALFANDGAALILTPIVLAMVRALNFDEKKVFPFIIASGFIADTTSLPLVVSN
LVNIVSADFFGISFSQYALIMWIPTLFSLVASILILYFYFRKALPKKYDMRVIREPASAIKDKKMFRVSWGILIILVAGY
FLSSFLDIPVSFIALLVAVIFLLVSAKSHAVSTKAILKGAPWNIVFFSVGMYVVVYGLQNVGITKLLSEAITYVAHFGLF
AGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSFTGVMKEALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKIS
WGSYFKIGIVITIPVLFITLVGLYFSLLIWN

Sequences:

>Translated_431_residues
MQITLAILVFAFTLFFVIWQPRKLSIGWSACGGALIAILLGVVTLSDVATVTGIVWNATLAFIAIIIISLILDKIGFFEW
AALHMARLARGNGLLMFILISILGAFVAALFANDGAALILTPIVLAMVRALNFDEKKVFPFIIASGFIADTTSLPLVVSN
LVNIVSADFFGISFSQYALIMWIPTLFSLVASILILYFYFRKALPKKYDMRVIREPASAIKDKKMFRVSWGILIILVAGY
FLSSFLDIPVSFIALLVAVIFLLVSAKSHAVSTKAILKGAPWNIVFFSVGMYVVVYGLQNVGITKLLSEAITYVAHFGLF
AGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSFTGVMKEALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKIS
WGSYFKIGIVITIPVLFITLVGLYFSLLIWN
>Mature_431_residues
MQITLAILVFAFTLFFVIWQPRKLSIGWSACGGALIAILLGVVTLSDVATVTGIVWNATLAFIAIIIISLILDKIGFFEW
AALHMARLARGNGLLMFILISILGAFVAALFANDGAALILTPIVLAMVRALNFDEKKVFPFIIASGFIADTTSLPLVVSN
LVNIVSADFFGISFSQYALIMWIPTLFSLVASILILYFYFRKALPKKYDMRVIREPASAIKDKKMFRVSWGILIILVAGY
FLSSFLDIPVSFIALLVAVIFLLVSAKSHAVSTKAILKGAPWNIVFFSVGMYVVVYGLQNVGITKLLSEAITYVAHFGLF
AGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSFTGVMKEALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKIS
WGSYFKIGIVITIPVLFITLVGLYFSLLIWN

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=423, Percent_Identity=57.4468085106383, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 46865; Mature: 46865

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITLAILVFAFTLFFVIWQPRKLSIGWSACGGALIAILLGVVTLSDVATVTGIVWNATL
CCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFIAIIIISLILDKIGFFEWAALHMARLARGNGLLMFILISILGAFVAALFANDGAALIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
TPIVLAMVRALNFDEKKVFPFIIASGFIADTTSLPLVVSNLVNIVSADFFGISFSQYALI
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
MWIPTLFSLVASILILYFYFRKALPKKYDMRVIREPASAIKDKKMFRVSWGILIILVAGY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLSSFLDIPVSFIALLVAVIFLLVSAKSHAVSTKAILKGAPWNIVFFSVGMYVVVYGLQN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
VGITKLLSEAITYVAHFGLFAGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSFTGVMK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
EALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKISWGSYFKIGIVITIPVLFITL
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEHHHHHHHHHHHHHH
VGLYFSLLIWN
HHHHHHHHHCC
>Mature Secondary Structure
MQITLAILVFAFTLFFVIWQPRKLSIGWSACGGALIAILLGVVTLSDVATVTGIVWNATL
CCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFIAIIIISLILDKIGFFEWAALHMARLARGNGLLMFILISILGAFVAALFANDGAALIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
TPIVLAMVRALNFDEKKVFPFIIASGFIADTTSLPLVVSNLVNIVSADFFGISFSQYALI
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
MWIPTLFSLVASILILYFYFRKALPKKYDMRVIREPASAIKDKKMFRVSWGILIILVAGY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLSSFLDIPVSFIALLVAVIFLLVSAKSHAVSTKAILKGAPWNIVFFSVGMYVVVYGLQN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
VGITKLLSEAITYVAHFGLFAGTVGMGFIAAILSSVMNNLPTVMINALAIDHTSFTGVMK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
EALVYANVIGSDLGPKITPIGSLATLLWLHVLAQKGIKISWGSYFKIGIVITIPVLFITL
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEHHHHHHHHHHHHHH
VGLYFSLLIWN
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]