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The map label for this gene is glnA

Identifier: 226223901

GI number: 226223901

Start: 1311425

End: 1312759

Strand: Direct

Name: glnA

Synonym: Lm4b_01308

Alternate gene names: 226223901

Gene position: 1311425-1312759 (Clockwise)

Preceding gene: 226223900

Following gene: 226223902

Centisome position: 45.02

GC content: 39.25

Gene sequence:

>1335_bases
ATGGCAAAATATACAAAAGAAGACATTTTCCGCTTCGCAGATGAACAAAATGTAAAATTTATTCGTTTACAGTTCACGGA
TATCCTCGGGATTATTAAGAATGTGGAAATTCCTGTTAGCCAACTAAAGAAAGCACTAGATAACAAAATCATGTTTGATG
GTTCCTCCATTGAAGGCTTTGTACGAATTGAAGAGTCTGATATGTATTTATTCCCAGACTTAGACACTTGGGTTGTGTTC
CCTTGGACAGCTGAAAAAGGAAAAGTTGCAAGAATGATTTGTGATATTTATAATCCTGACATGACTCCTTTTGCTGGAGA
CCCACGTGCTAACTTAAAACGTGTACTTAAAGAAATGGAAGAACTAGGTTTCACAGAGTTCAACTTAGGACCTGAGCCAG
AATTTTTCCTATTCAAATTAGATGAAAATCGTCGTCCGACACTTGAACTAAATGATAGTGGTGGTTACTTCGATTTAGCA
CCAACCGACTTAGGTGAAAACTGTCGTCGCGATATTGTGCTTGAATTAGAAGAAATGGGCTTTGAAATCGAAGCGTCTCA
CCATGAAGTGGCACCAGGACAACACGAAATTGACTTTAAATATGAAGACGCCGTTACAGCGTGTGATAGCATCCAAACAT
TTAAATTAGTCGTAAAAACAATTGCTCGTAAACATGGTTTACACGCAACATTCATGCCGAAGCCGCTCTTTGGTGTAAAT
GGTTCTGGAATGCATTTCAATATGTCACTATTTAATGAAAAAGGAAATGCTTTCTTCGATGAAAGCGGAGAACTAGAATT
GAGCCAAACAGCTTATCATTTCTTAGCAGGGATGTTAAAACATGCCCGTGGCTATACAGCTGTTACAAATCCAACCATTA
ACTCATTCAAACGATTGGTTCCAGGATATGAAGCACCATGTTACATTGCGTGGTCAGGTAAAAATCGTAGCCCGCTTGTT
CGTGTTCCAAGTTCTCGTGGACTAAGCACTCGTCTTGAATTACGTAGCGTCGATCCATCTGCTAATCCATATTTAGCAAT
GGCGGTACTACTTAAAGCTGGTTTATCTGGAATTAAAGATGAATTAACTCCTCCGGCACCAGTTGATCGTAACATTTACG
GCATGAACGAAGAAGAACGTGAAGCAACTGGCATTTATGATCTACCAGAAAGCTTGGGCCACGCCTTAATCGAACTTGAA
AAAGACGATATTATCAAAGCTGGTTTAGGTGAGCACATTTTCGAACACTTTATTGAAGCAAAAACAATTGAGTGTGATAT
GTTCCGCACAGCAGTTCACCCTTGGGAACGCGAACAATATCTTGAAATTTATTAA

Upstream 100 bases:

>100_bases
ACAGGGAAACAACAATTACCAAGGTTTTAAAAAGACAAGAAGAGGTATTTACTTTATTTAGAAGTTAATATAATTAGATT
ATTTAGGAGGAATTTAGATT

Downstream 100 bases:

>100_bases
TAGTCAAAAGCACGTTTCCTATAGATGGAAACGTGCTTTTTTATTGGAAAATCGGTTTCAAATGTTTGATGTTTGGCAAA
TAGAGGCATATATTTAAAAA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGFVRIEESDMYLFPDLDTWVVF
PWTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEMEELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYEDAVTACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVN
GSGMHFNMSLFNEKGNAFFDESGELELSQTAYHFLAGMLKHARGYTAVTNPTINSFKRLVPGYEAPCYIAWSGKNRSPLV
RVPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKDELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELE
KDDIIKAGLGEHIFEHFIEAKTIECDMFRTAVHPWEREQYLEIY

Sequences:

>Translated_444_residues
MAKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGFVRIEESDMYLFPDLDTWVVF
PWTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEMEELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYEDAVTACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVN
GSGMHFNMSLFNEKGNAFFDESGELELSQTAYHFLAGMLKHARGYTAVTNPTINSFKRLVPGYEAPCYIAWSGKNRSPLV
RVPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKDELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELE
KDDIIKAGLGEHIFEHFIEAKTIECDMFRTAVHPWEREQYLEIY
>Mature_443_residues
AKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGFVRIEESDMYLFPDLDTWVVFP
WTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEMEELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLAP
TDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYEDAVTACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVNG
SGMHFNMSLFNEKGNAFFDESGELELSQTAYHFLAGMLKHARGYTAVTNPTINSFKRLVPGYEAPCYIAWSGKNRSPLVR
VPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKDELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELEK
DDIIKAGLGEHIFEHFIEAKTIECDMFRTAVHPWEREQYLEIY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=458, Percent_Identity=25.5458515283843, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1790301, Length=477, Percent_Identity=38.3647798742138, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI87081870, Length=458, Percent_Identity=29.2576419213974, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50462; Mature: 50331

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGF
CCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLFPDLDTWVVFPWTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEME
EEEECCCEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLAPTDLGENCRRDIVLELEEMG
HCCCEEECCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCHHHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYEDAVTACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVN
EEEECCCCCCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHFNMSLFNEKGNAFFDESGELELSQTAYHFLAGMLKHARGYTAVTNPTINSFKRLV
CCCEEEEEEEECCCCCEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHC
PGYEAPCYIAWSGKNRSPLVRVPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKD
CCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELEKDDIIKAGLGEHIFEHFIEA
CCCCCCCCCCCEECCCHHHHHHCCCCCCHHHHCHHEEEECCHHHHHHCHHHHHHHHHHHC
KTIECDMFRTAVHPWEREQYLEIY
CCEEHHHHHHHCCCCCCCCCEECC
>Mature Secondary Structure 
AKYTKEDIFRFADEQNVKFIRLQFTDILGIIKNVEIPVSQLKKALDNKIMFDGSSIEGF
CCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLFPDLDTWVVFPWTAEKGKVARMICDIYNPDMTPFAGDPRANLKRVLKEME
EEEECCCEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ELGFTEFNLGPEPEFFLFKLDENRRPTLELNDSGGYFDLAPTDLGENCRRDIVLELEEMG
HCCCEEECCCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCHHHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYEDAVTACDSIQTFKLVVKTIARKHGLHATFMPKPLFGVN
EEEECCCCCCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHFNMSLFNEKGNAFFDESGELELSQTAYHFLAGMLKHARGYTAVTNPTINSFKRLV
CCCEEEEEEEECCCCCEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHC
PGYEAPCYIAWSGKNRSPLVRVPSSRGLSTRLELRSVDPSANPYLAMAVLLKAGLSGIKD
CCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ELTPPAPVDRNIYGMNEEEREATGIYDLPESLGHALIELEKDDIIKAGLGEHIFEHFIEA
CCCCCCCCCCCEECCCHHHHHHCCCCCCHHHHCHHEEEECCHHHHHHCHHHHHHHHHHHC
KTIECDMFRTAVHPWEREQYLEIY
CCEEHHHHHHHCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2572584 [H]