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The map label for this gene is hslU

Identifier: 226223882

GI number: 226223882

Start: 1289348

End: 1290757

Strand: Direct

Name: hslU

Synonym: Lm4b_01289

Alternate gene names: 226223882

Gene position: 1289348-1290757 (Clockwise)

Preceding gene: 226223881

Following gene: 226223883

Centisome position: 44.27

GC content: 37.23

Gene sequence:

>1410_bases
TTGACAAATATAACGTTAATGAACCAGTTGACACCAAAGCAAATTGTTGAAAAACTGGATCAATATATTATTGGACAAAC
CGGAGCGAAGAAATCAGTTGCAGTAGCACTAAGAAATCGTTACCGTAGACAACTAATGGATGAATCAATCCGTGATGAAA
TTATTCCGAAAAACATTCTGATGATTGGACCAACAGGTGTTGGTAAAACAGAAATAGCACGTCGAATTGCCAAAATTGTT
CGTGCTCCTTTTTCCAAAGTAGAGGCTACTAAATTCACGGAAGTTGGCTATGTCGGTCGCGATGTAGAATCAATGGTTCG
TGACTTAGTGGAGGTATCTGTTCGTTTAGTAAAAGAAGAAAAAATGCAATTAGTTCGTGTTAAAGCAGAAAAAAATGCAG
AGAAACGTCTGATTAAACTTTTAGCGCCAAGCCAAAAGAAAAAACAAACAACATCGCAAAATCCGTTAGAAGCGCTTTTC
GGCGGCATGAATCAACCAGATGAATCTCCTGAGGAAGAAGTAGATCAAGAATTAAAGAATAAACGTAGCCAAATTGAATG
GCGTCTTCAAAACGGCGAGCTTGATGATGAAATAGTAACAGTAGAAGTAAAAGAACAGCAAAATCCAATGCTTGATATGA
TGCGCGGCGCTGGAATGGACCAAATGAATGGTATGCAGGATGCGCTTTCGGGCATGTTCCCAGCGAAAAAGAAAAAACGC
AAAGTAACCGTACGTGAAGCGAAGAAAATTCTTTTTGAAGATGAAGCATCAAAATTAATTGATGCAGATGAACTTGCAGC
AGAGGGCATTCATCGTGCGGAACAAATGGGTATGATTTTTATCGATGAAATTGATAAAATTGCTAGTAAAGAAGGCGGCG
GAAATGCACAAGTTTCGCGGGAAGGCGTCCAAAGAGACATTCTTCCAATCGTGGAGGGGTCGCAAATTTCTACTAAATAT
GGTACAGTCAACACGGAGTACATTTTATTTATCGCAGCAGGGGCTTTCCATATGTCTAAACCAAGCGATTTAATTCCAGA
GCTTCAAGGCCGGTTCCCAATTAGAATTGAGCTGGATAAATTAACTCAAGAAGATTTCTACAAAATCTTAACAGAACCAG
ACAATGCACTAATTAAACAATACAAAGCTTTACTGAAAACAGAAGGAATTGACTTGATTTTCACCAAAGAAGCCGTAGAA
AGAATTGCCGAGATTGCATTCCAAGTTAATCAAGATTCAGATAATATCGGAGCAAGAAGACTTCATACCATTTTAGAAAA
ATTACTAGAAGATTTACTATTTGAAGCACCAGAAATCAATATGGAATCTATTAAAGTAACAGAAAACTATGTTAATGAAA
AACTTGCACCAATTATGCAGAATAAAGATTTAACACAATTTATTTTATAA

Upstream 100 bases:

>100_bases
GCAAAGGATATCGCTCGTCATGCGCTAGAAATCGCTTCTGAAATTTGTGTGTTTACAAATGATCATATTACAGTAGAAGA
GCTTTGAAGGAGTGGTTAAT

Downstream 100 bases:

>100_bases
AATACTAGGAGGATCTAATAATGACTTTATTAGAAAAAACAAGAAAAATTAATGCTATGTTACAAAATGCTGCAGGAAAA
ACAGTAAACTTCAAAGAAAT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTNITLMNQLTPKQIVEKLDQYIIGQTGAKKSVAVALRNRYRRQLMDESIRDEIIPKNILMIGPTGVGKTEIARRIAKIV
RAPFSKVEATKFTEVGYVGRDVESMVRDLVEVSVRLVKEEKMQLVRVKAEKNAEKRLIKLLAPSQKKKQTTSQNPLEALF
GGMNQPDESPEEEVDQELKNKRSQIEWRLQNGELDDEIVTVEVKEQQNPMLDMMRGAGMDQMNGMQDALSGMFPAKKKKR
KVTVREAKKILFEDEASKLIDADELAAEGIHRAEQMGMIFIDEIDKIASKEGGGNAQVSREGVQRDILPIVEGSQISTKY
GTVNTEYILFIAAGAFHMSKPSDLIPELQGRFPIRIELDKLTQEDFYKILTEPDNALIKQYKALLKTEGIDLIFTKEAVE
RIAEIAFQVNQDSDNIGARRLHTILEKLLEDLLFEAPEINMESIKVTENYVNEKLAPIMQNKDLTQFIL

Sequences:

>Translated_469_residues
MTNITLMNQLTPKQIVEKLDQYIIGQTGAKKSVAVALRNRYRRQLMDESIRDEIIPKNILMIGPTGVGKTEIARRIAKIV
RAPFSKVEATKFTEVGYVGRDVESMVRDLVEVSVRLVKEEKMQLVRVKAEKNAEKRLIKLLAPSQKKKQTTSQNPLEALF
GGMNQPDESPEEEVDQELKNKRSQIEWRLQNGELDDEIVTVEVKEQQNPMLDMMRGAGMDQMNGMQDALSGMFPAKKKKR
KVTVREAKKILFEDEASKLIDADELAAEGIHRAEQMGMIFIDEIDKIASKEGGGNAQVSREGVQRDILPIVEGSQISTKY
GTVNTEYILFIAAGAFHMSKPSDLIPELQGRFPIRIELDKLTQEDFYKILTEPDNALIKQYKALLKTEGIDLIFTKEAVE
RIAEIAFQVNQDSDNIGARRLHTILEKLLEDLLFEAPEINMESIKVTENYVNEKLAPIMQNKDLTQFIL
>Mature_468_residues
TNITLMNQLTPKQIVEKLDQYIIGQTGAKKSVAVALRNRYRRQLMDESIRDEIIPKNILMIGPTGVGKTEIARRIAKIVR
APFSKVEATKFTEVGYVGRDVESMVRDLVEVSVRLVKEEKMQLVRVKAEKNAEKRLIKLLAPSQKKKQTTSQNPLEALFG
GMNQPDESPEEEVDQELKNKRSQIEWRLQNGELDDEIVTVEVKEQQNPMLDMMRGAGMDQMNGMQDALSGMFPAKKKKRK
VTVREAKKILFEDEASKLIDADELAAEGIHRAEQMGMIFIDEIDKIASKEGGGNAQVSREGVQRDILPIVEGSQISTKYG
TVNTEYILFIAAGAFHMSKPSDLIPELQGRFPIRIELDKLTQEDFYKILTEPDNALIKQYKALLKTEGIDLIFTKEAVER
IAEIAFQVNQDSDNIGARRLHTILEKLLEDLLFEAPEINMESIKVTENYVNEKLAPIMQNKDLTQFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=114, Percent_Identity=36.8421052631579, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1790366, Length=465, Percent_Identity=47.3118279569892, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1786642, Length=240, Percent_Identity=30.8333333333333, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71982908, Length=245, Percent_Identity=30.6122448979592, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71982905, Length=245, Percent_Identity=30.6122448979592, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988663, Length=258, Percent_Identity=25.968992248062, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319704, Length=123, Percent_Identity=34.9593495934959, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648289, Length=249, Percent_Identity=30.1204819277108, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648291, Length=249, Percent_Identity=30.1204819277108, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 53189; Mature: 53058

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNITLMNQLTPKQIVEKLDQYIIGQTGAKKSVAVALRNRYRRQLMDESIRDEIIPKNIL
CCCEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
MIGPTGVGKTEIARRIAKIVRAPFSKVEATKFTEVGYVGRDVESMVRDLVEVSVRLVKEE
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
KMQLVRVKAEKNAEKRLIKLLAPSQKKKQTTSQNPLEALFGGMNQPDESPEEEVDQELKN
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHH
KRSQIEWRLQNGELDDEIVTVEVKEQQNPMLDMMRGAGMDQMNGMQDALSGMFPAKKKKR
HHHHEEEEECCCCCCCCEEEEEECCCCCHHHHHHHCCCCHHHCCHHHHHHCCCCCHHHHH
KVTVREAKKILFEDEASKLIDADELAAEGIHRAEQMGMIFIDEIDKIASKEGGGNAQVSR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHH
EGVQRDILPIVEGSQISTKYGTVNTEYILFIAAGAFHMSKPSDLIPELQGRFPIRIELDK
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCEEEEHHH
LTQEDFYKILTEPDNALIKQYKALLKTEGIDLIFTKEAVERIAEIAFQVNQDSDNIGARR
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHHHH
LHTILEKLLEDLLFEAPEINMESIKVTENYVNEKLAPIMQNKDLTQFIL
HHHHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
TNITLMNQLTPKQIVEKLDQYIIGQTGAKKSVAVALRNRYRRQLMDESIRDEIIPKNIL
CCEEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
MIGPTGVGKTEIARRIAKIVRAPFSKVEATKFTEVGYVGRDVESMVRDLVEVSVRLVKEE
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
KMQLVRVKAEKNAEKRLIKLLAPSQKKKQTTSQNPLEALFGGMNQPDESPEEEVDQELKN
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHH
KRSQIEWRLQNGELDDEIVTVEVKEQQNPMLDMMRGAGMDQMNGMQDALSGMFPAKKKKR
HHHHEEEEECCCCCCCCEEEEEECCCCCHHHHHHHCCCCHHHCCHHHHHHCCCCCHHHHH
KVTVREAKKILFEDEASKLIDADELAAEGIHRAEQMGMIFIDEIDKIASKEGGGNAQVSR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHH
EGVQRDILPIVEGSQISTKYGTVNTEYILFIAAGAFHMSKPSDLIPELQGRFPIRIELDK
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCEEEEHHH
LTQEDFYKILTEPDNALIKQYKALLKTEGIDLIFTKEAVERIAEIAFQVNQDSDNIGARR
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHHHH
LHTILEKLLEDLLFEAPEINMESIKVTENYVNEKLAPIMQNKDLTQFIL
HHHHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]