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The map label for this gene is proB

Identifier: 226223863

GI number: 226223863

Start: 1270952

End: 1271782

Strand: Reverse

Name: proB

Synonym: Lm4b_01270

Alternate gene names: 226223863

Gene position: 1271782-1270952 (Counterclockwise)

Preceding gene: 226223864

Following gene: 226223862

Centisome position: 43.66

GC content: 37.55

Gene sequence:

>831_bases
ATGCGCGAATCTTTAAAAAATAGTAAGAGATTAGTCATCAAAGTTGGTACAAGTACATTAATGTATGGAAATGGCCATAT
TAATTTACGTACTATAGAAAAATTAGCAATGGTTTTATCCGATTTACGTAACGAAGGAAAAGAGGTCATTCTTGTTTCTT
CTGGGGCAATCGGTGTTGGTTGTCATAAATTACAGCTTTCAGTAAGACCTACAAGTATCCCTGACCTTCAAGCTGTCGCG
TCGGTTGGTCAAAGTGAACTAATGCATATTTATAGTAAATTTTTTGGTGAATATGGACAAGTGGTTGGACAGGTGTTGCT
TACACGTGATGTAACTGACTTCCCTATTAGCCGCGAAAATGTTATGAATACTTTAGACAGTTTATTAAGCCGTGGTATTA
TCCCTATCGTAAATGAAAATGATACCGTTGCTGTAGAAGAACTGGAGCATGTAACGAAATACGGGGATAATGATCTTCTT
TCAGCCATTGTCGCTAAACTGGTTCAAGCTGATTTGCTTATTATGCTTTCTGATATTGACGGCTTTTATGGTAGTAACCC
TGCAACAGATCCAGAGGCTGTTATGTTCTCTGAAATCAACCAAATCACTCCAGAAATTGAAGCCCTTGCTGGAGGACGCG
GTTCTAAATTTGGGACTGGTGGTATGTTGACCAAGCTTTCTGCTGCTTCTTATTGTATGGATTCTAACCAAAAAATGATT
TTAACAAACGGTAAAAACCCTACCGTCATTTTTAATATTATGCAAGGTGAACAAATTGGTACTTTATTCGCAAGTAAAAA
GGAGGAACTTTCACATGACCGAACTCATTAA

Upstream 100 bases:

>100_bases
AATTAAAAATCTGAAGCTCATAAACTTGATTCTAATATGGACATATCTGCATTTTCATGTAAAATAGAAGTATTATCTTA
TTAGCAGAGGGGTCATAGAA

Downstream 100 bases:

>100_bases
AAAAGGGAGTGGCGCTAAAGAAGCTTCTCAATTTTTAGCCCAAGCAACAACAAAACACAAAAACGCAGCACTGCTTAATT
TAAGTAATGATTTGCTTATC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRESLKNSKRLVIKVGTSTLMYGNGHINLRTIEKLAMVLSDLRNEGKEVILVSSGAIGVGCHKLQLSVRPTSIPDLQAVA
SVGQSELMHIYSKFFGEYGQVVGQVLLTRDVTDFPISRENVMNTLDSLLSRGIIPIVNENDTVAVEELEHVTKYGDNDLL
SAIVAKLVQADLLIMLSDIDGFYGSNPATDPEAVMFSEINQITPEIEALAGGRGSKFGTGGMLTKLSAASYCMDSNQKMI
LTNGKNPTVIFNIMQGEQIGTLFASKKEELSHDRTH

Sequences:

>Translated_276_residues
MRESLKNSKRLVIKVGTSTLMYGNGHINLRTIEKLAMVLSDLRNEGKEVILVSSGAIGVGCHKLQLSVRPTSIPDLQAVA
SVGQSELMHIYSKFFGEYGQVVGQVLLTRDVTDFPISRENVMNTLDSLLSRGIIPIVNENDTVAVEELEHVTKYGDNDLL
SAIVAKLVQADLLIMLSDIDGFYGSNPATDPEAVMFSEINQITPEIEALAGGRGSKFGTGGMLTKLSAASYCMDSNQKMI
LTNGKNPTVIFNIMQGEQIGTLFASKKEELSHDRTH
>Mature_276_residues
MRESLKNSKRLVIKVGTSTLMYGNGHINLRTIEKLAMVLSDLRNEGKEVILVSSGAIGVGCHKLQLSVRPTSIPDLQAVA
SVGQSELMHIYSKFFGEYGQVVGQVLLTRDVTDFPISRENVMNTLDSLLSRGIIPIVNENDTVAVEELEHVTKYGDNDLL
SAIVAKLVQADLLIMLSDIDGFYGSNPATDPEAVMFSEINQITPEIEALAGGRGSKFGTGGMLTKLSAASYCMDSNQKMI
LTNGKNPTVIFNIMQGEQIGTLFASKKEELSHDRTH

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=32.3232323232323, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=32.1070234113712, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1786437, Length=274, Percent_Identity=38.3211678832117, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=32.9749103942652, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=32.9749103942652, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=262, Percent_Identity=38.1679389312977, Blast_Score=165, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6321822, Length=248, Percent_Identity=37.9032258064516, Blast_Score=162, Evalue=4e-41,
Organism=Drosophila melanogaster, GI21357643, Length=297, Percent_Identity=35.016835016835, Blast_Score=157, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 30033; Mature: 30033

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRESLKNSKRLVIKVGTSTLMYGNGHINLRTIEKLAMVLSDLRNEGKEVILVSSGAIGVG
CCCCCCCCCEEEEEECCCEEEEECCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEC
CHKLQLSVRPTSIPDLQAVASVGQSELMHIYSKFFGEYGQVVGQVLLTRDVTDFPISREN
EEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VMNTLDSLLSRGIIPIVNENDTVAVEELEHVTKYGDNDLLSAIVAKLVQADLLIMLSDID
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCC
GFYGSNPATDPEAVMFSEINQITPEIEALAGGRGSKFGTGGMLTKLSAASYCMDSNQKMI
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
LTNGKNPTVIFNIMQGEQIGTLFASKKEELSHDRTH
EECCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRESLKNSKRLVIKVGTSTLMYGNGHINLRTIEKLAMVLSDLRNEGKEVILVSSGAIGVG
CCCCCCCCCEEEEEECCCEEEEECCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEC
CHKLQLSVRPTSIPDLQAVASVGQSELMHIYSKFFGEYGQVVGQVLLTRDVTDFPISREN
EEEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VMNTLDSLLSRGIIPIVNENDTVAVEELEHVTKYGDNDLLSAIVAKLVQADLLIMLSDID
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCC
GFYGSNPATDPEAVMFSEINQITPEIEALAGGRGSKFGTGGMLTKLSAASYCMDSNQKMI
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
LTNGKNPTVIFNIMQGEQIGTLFASKKEELSHDRTH
EECCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]