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The map label for this gene is proA

Identifier: 226223862

GI number: 226223862

Start: 1269721

End: 1270968

Strand: Reverse

Name: proA

Synonym: Lm4b_01269

Alternate gene names: 226223862

Gene position: 1270968-1269721 (Counterclockwise)

Preceding gene: 226223863

Following gene: 226223860

Centisome position: 43.64

GC content: 39.26

Gene sequence:

>1248_bases
ATGACCGAACTCATTAAAAAAGGGAGTGGCGCTAAAGAAGCTTCTCAATTTTTAGCCCAAGCAACAACAAAACACAAAAA
CGCAGCACTGCTTAATTTAAGTAATGATTTGCTTATCCATACGGCTACACTTTTGCAAGAAAATGAAAAGGACATTCTTC
GCGCTAAGCAAAAAGGAACACCTGAAACGATGATTGATCGTCTTCGTCTTACAGAAGAAAGAATCAAAGAGATATCGGAA
GCGGTTAAACAAGTGGTCGCGTTAAAAGATCCAATCGGTGAAGTAACAAATATGTGGAAAAATGAAGCCGAGTTAACTAT
TGGTAAAACAAGAGTTCCACTTGGTGTTATTGGTATTATTTATGAATCTAGGCCCAATGTAACTGTCGATGCTTCTGTTC
TTTGTTTTAAAACTGGAAATGCGGTGATTTTGCGAGGTGGTAGTGATGCTATTGATTCCAATAAAGCATTAATGTCCGTT
ATCCAGGATTCTCTTGAGGCATCTGGCTTTCCTCGTTCTAGTGTTCAATTAATTGAAGATACTTCTCGCGAAACTGCCCA
AGACATGATGCGGCTAAATCGCTTTTTAGATGTGCTCATCCCTCGTGGTGGCGCAAGGTTAATTCAAACAGTTCTTGAAA
ATGCAACTGTTCCAGTCATCGAAACTGGTACTGGTAATTGTCATATTTACGTGGATAAAGCAGCAGAAAAACAAATGGCT
ATAGATATTTTAGTTAATGCTAAGTGTTCGCGCCCTTCTGTATGCAATGCTGCAGAAACTTTGCTCATTCATCGAGATGT
TGCCGAAGATTATCTTCCAGCGATGGAAACTGCATTAAAAGAATATGATGTCGAACTACGTGCGGATGAACGTGCGAAGG
GAATTTTACAAGAGGCAAAAGCTGCGACAGAGTCCGACTGGGAAGATGAATTTTTAGATTTCATTTTAGCTATTAAAGTA
GTCGATTCCGCTGAAGAGGCTATTGATCATATTAATAAATATGGCACCAAGCACTCTGAAGCAATTATTTCTAATGATTA
CGCGACTGGTCAAGCATTCCATCAAAAAGTAGACGCAGCAGCCGTTTATATCAATGCTTCTACTCGATTCACTGATGGTT
TTGCCATGGGATTTGGCGCTGAAATTGGCATTAGTACACAAAAACTTCATGCACGTGGACCAATGGGCTTAACAGAACTT
ACATCGACTAAATATATTATTTTTGGAGACGGTCAAATCCGTAATTAA

Upstream 100 bases:

>100_bases
ATGATTTTAACAAACGGTAAAAACCCTACCGTCATTTTTAATATTATGCAAGGTGAACAAATTGGTACTTTATTCGCAAG
TAAAAAGGAGGAACTTTCAC

Downstream 100 bases:

>100_bases
AAAGAAGGTAGCCTATGATTTTAGGCTACCTTCTTTTTTCATCCAACCAATCAATTACATCTTGAAAAACAGTTTCTTTA
CATAATTCATTCACCAGTTC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTELIKKGSGAKEASQFLAQATTKHKNAALLNLSNDLLIHTATLLQENEKDILRAKQKGTPETMIDRLRLTEERIKEISE
AVKQVVALKDPIGEVTNMWKNEAELTIGKTRVPLGVIGIIYESRPNVTVDASVLCFKTGNAVILRGGSDAIDSNKALMSV
IQDSLEASGFPRSSVQLIEDTSRETAQDMMRLNRFLDVLIPRGGARLIQTVLENATVPVIETGTGNCHIYVDKAAEKQMA
IDILVNAKCSRPSVCNAAETLLIHRDVAEDYLPAMETALKEYDVELRADERAKGILQEAKAATESDWEDEFLDFILAIKV
VDSAEEAIDHINKYGTKHSEAIISNDYATGQAFHQKVDAAAVYINASTRFTDGFAMGFGAEIGISTQKLHARGPMGLTEL
TSTKYIIFGDGQIRN

Sequences:

>Translated_415_residues
MTELIKKGSGAKEASQFLAQATTKHKNAALLNLSNDLLIHTATLLQENEKDILRAKQKGTPETMIDRLRLTEERIKEISE
AVKQVVALKDPIGEVTNMWKNEAELTIGKTRVPLGVIGIIYESRPNVTVDASVLCFKTGNAVILRGGSDAIDSNKALMSV
IQDSLEASGFPRSSVQLIEDTSRETAQDMMRLNRFLDVLIPRGGARLIQTVLENATVPVIETGTGNCHIYVDKAAEKQMA
IDILVNAKCSRPSVCNAAETLLIHRDVAEDYLPAMETALKEYDVELRADERAKGILQEAKAATESDWEDEFLDFILAIKV
VDSAEEAIDHINKYGTKHSEAIISNDYATGQAFHQKVDAAAVYINASTRFTDGFAMGFGAEIGISTQKLHARGPMGLTEL
TSTKYIIFGDGQIRN
>Mature_414_residues
TELIKKGSGAKEASQFLAQATTKHKNAALLNLSNDLLIHTATLLQENEKDILRAKQKGTPETMIDRLRLTEERIKEISEA
VKQVVALKDPIGEVTNMWKNEAELTIGKTRVPLGVIGIIYESRPNVTVDASVLCFKTGNAVILRGGSDAIDSNKALMSVI
QDSLEASGFPRSSVQLIEDTSRETAQDMMRLNRFLDVLIPRGGARLIQTVLENATVPVIETGTGNCHIYVDKAAEKQMAI
DILVNAKCSRPSVCNAAETLLIHRDVAEDYLPAMETALKEYDVELRADERAKGILQEAKAATESDWEDEFLDFILAIKVV
DSAEEAIDHINKYGTKHSEAIISNDYATGQAFHQKVDAAAVYINASTRFTDGFAMGFGAEIGISTQKLHARGPMGLTELT
STKYIIFGDGQIRN

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=388, Percent_Identity=35.0515463917526, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI62912457, Length=388, Percent_Identity=35.0515463917526, Blast_Score=250, Evalue=1e-66,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=46.2650602409639, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17569969, Length=407, Percent_Identity=34.8894348894349, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17569967, Length=407, Percent_Identity=34.8894348894349, Blast_Score=266, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324899, Length=424, Percent_Identity=34.4339622641509, Blast_Score=256, Evalue=3e-69,
Organism=Drosophila melanogaster, GI21357643, Length=394, Percent_Identity=34.7715736040609, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45465; Mature: 45334

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELIKKGSGAKEASQFLAQATTKHKNAALLNLSNDLLIHTATLLQENEKDILRAKQKGT
CCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHEEHHHHHHCCHHHHHHHHCCCC
PETMIDRLRLTEERIKEISEAVKQVVALKDPIGEVTNMWKNEAELTIGKTRVPLGVIGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCEEEEEEE
YESRPNVTVDASVLCFKTGNAVILRGGSDAIDSNKALMSVIQDSLEASGFPRSSVQLIED
EECCCCEEEEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
TSRETAQDMMRLNRFLDVLIPRGGARLIQTVLENATVPVIETGTGNCHIYVDKAAEKQMA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCEE
IDILVNAKCSRPSVCNAAETLLIHRDVAEDYLPAMETALKEYDVELRADERAKGILQEAK
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHH
AATESDWEDEFLDFILAIKVVDSAEEAIDHINKYGTKHSEAIISNDYATGQAFHQKVDAA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHCEE
AVYINASTRFTDGFAMGFGAEIGISTQKLHARGPMGLTELTSTKYIIFGDGQIRN
EEEEECCCEECCCEEECCCCCCCCCHHHHHCCCCCCHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure 
TELIKKGSGAKEASQFLAQATTKHKNAALLNLSNDLLIHTATLLQENEKDILRAKQKGT
CCHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHEEHHHHHHCCHHHHHHHHCCCC
PETMIDRLRLTEERIKEISEAVKQVVALKDPIGEVTNMWKNEAELTIGKTRVPLGVIGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCEEEEEEE
YESRPNVTVDASVLCFKTGNAVILRGGSDAIDSNKALMSVIQDSLEASGFPRSSVQLIED
EECCCCEEEEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
TSRETAQDMMRLNRFLDVLIPRGGARLIQTVLENATVPVIETGTGNCHIYVDKAAEKQMA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCEE
IDILVNAKCSRPSVCNAAETLLIHRDVAEDYLPAMETALKEYDVELRADERAKGILQEAK
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHH
AATESDWEDEFLDFILAIKVVDSAEEAIDHINKYGTKHSEAIISNDYATGQAFHQKVDAA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHCEE
AVYINASTRFTDGFAMGFGAEIGISTQKLHARGPMGLTELTSTKYIIFGDGQIRN
EEEEECCCEECCCEEECCCCCCCCCHHHHHCCCCCCHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]