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The map label for this gene is phr [H]

Identifier: 226223217

GI number: 226223217

Start: 632729

End: 634132

Strand: Direct

Name: phr [H]

Synonym: Lm4b_00614

Alternate gene names: 226223217

Gene position: 632729-634132 (Clockwise)

Preceding gene: 226223216

Following gene: 226223218

Centisome position: 21.72

GC content: 39.46

Gene sequence:

>1404_bases
ATGACTTCGGTAATGTGGTTTCGGAGAGATCTTCGAGTAAACGATAATAAAGCTCTCTATCATGCTTGTAAAGAGGACGA
TTTGCTTTTGTTATTTCAAGTAAATCCAGCACAGTTCATCACAGGAAGTCCTAGCCACCAAGCTTTTTTTGCAAGTGTGG
CTCATTTTAAGCAAGAAATCGATAAAACTGCGCATTTACAAATCATGTTTGGTGAACCGATAGAATGCTTCCAACAACTT
AAAGATTCGCTACCAAGCTGGGATAAAGTTTATTTCAACCGTGATGAAACAGGATACGGAGCAAAGCGGGATGAGGCGGC
GCAAGCGTTCTTTGACGAACAAAAAATCGAGGTTCAAGCCTTCCACGATAGCTATCTTCATTCAGCGGAAGAAGTAAAGA
AATCTCCAACAGAATACTATAAAATTTTTACCCCTTATTATAAAAAATGGCGCGAAGAAATAAAAGAAACGCCGTTCAAA
GTGACTTTAAAGCATGAGAATATCCGGAAAGAAAGCTTGTTTCTGAAATATGAAGAACAATTTGCGGAAATGACTAGCGA
TTTACCGATATTAGATATTGGTGAACGAGCAGCGAACACGAGACTTGCGAATTTTATTAAACACGATGTTGCTGACTATG
ACAAAGCGAGAGATTTCCCGGAACTGGATAAAACAAGCCATTTATCACGTTATTTACGAACGGGGGAAATCTCGATTCGG
ACGATTTGGCAGACACTTCAAGAAACAGAAGCAACAGAAGGACGAGCTACATTTGAAAAAGAACTGTGTTGGCGCGACTT
TTATAATATGATTTACGTTTCTTTTCCTAACCAAAAAAACGAGCCGATCCAAGAAAATTATCGTTTTATTGAATGGGAAA
ATAACCGTGAATATTTCAAAGCGTGGCAAGAAGGGAAGACTGGTTTCCCGCTCGTTGATGCGGCGATGCGCCAGTTGAAA
GAAACTGGTTGGATGCATAATCGACTAAGAATGATTACGGCGTCCTTTTTAACAAAAGATTTGCTGATTGATTGGCGTTT
TGGCGAAAAGTATTTTCAACAAATGCTGATTGATTATGATCCTGCGAGTAATATTGGTGGCTGGCAATGGGCGGCTTCGA
CTGGAACGGATGCGGTGCCATACTTTAGGATTTTCAACCCAACGACACAATCACAAAAATTTGATTCGACTGGGAAATTT
ATTCGAAAATATGTAAAAGAGTTAGCGAATCTACCTGATAAATATATTCATCAACCAGAAAAAATGTCAGAAACTGAGCA
GAAAGAGCATGGCTTGCTGTTAGGAAAAGACTATCCATTTCCGATAGTTGACCATAAAGAACGACGGAAATTAGCCATTG
CACGCTACGAGTTTAGCAAAGAGCATTCTAGGGGAAATATATAG

Upstream 100 bases:

>100_bases
CAAAAGTTAACAGGTTTTTTCATGTTATAAGCCCATTGTTTTCTAAATACACTAGTTTCTCGTATAATAGCGTTAAGTAA
TTTGCGAAAGGTGGAAGAAA

Downstream 100 bases:

>100_bases
ATAATGAGTCAATCATTTAATTAAATGATTGATTTTTTTGTGGCAGTATTCACAAAGAATAAGTCTTGAGCAACCTTCTA
GGAGTAAGAACCTGGGTATG

Product: DNA photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTSVMWFRRDLRVNDNKALYHACKEDDLLLLFQVNPAQFITGSPSHQAFFASVAHFKQEIDKTAHLQIMFGEPIECFQQL
KDSLPSWDKVYFNRDETGYGAKRDEAAQAFFDEQKIEVQAFHDSYLHSAEEVKKSPTEYYKIFTPYYKKWREEIKETPFK
VTLKHENIRKESLFLKYEEQFAEMTSDLPILDIGERAANTRLANFIKHDVADYDKARDFPELDKTSHLSRYLRTGEISIR
TIWQTLQETEATEGRATFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNREYFKAWQEGKTGFPLVDAAMRQLK
ETGWMHNRLRMITASFLTKDLLIDWRFGEKYFQQMLIDYDPASNIGGWQWAASTGTDAVPYFRIFNPTTQSQKFDSTGKF
IRKYVKELANLPDKYIHQPEKMSETEQKEHGLLLGKDYPFPIVDHKERRKLAIARYEFSKEHSRGNI

Sequences:

>Translated_467_residues
MTSVMWFRRDLRVNDNKALYHACKEDDLLLLFQVNPAQFITGSPSHQAFFASVAHFKQEIDKTAHLQIMFGEPIECFQQL
KDSLPSWDKVYFNRDETGYGAKRDEAAQAFFDEQKIEVQAFHDSYLHSAEEVKKSPTEYYKIFTPYYKKWREEIKETPFK
VTLKHENIRKESLFLKYEEQFAEMTSDLPILDIGERAANTRLANFIKHDVADYDKARDFPELDKTSHLSRYLRTGEISIR
TIWQTLQETEATEGRATFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNREYFKAWQEGKTGFPLVDAAMRQLK
ETGWMHNRLRMITASFLTKDLLIDWRFGEKYFQQMLIDYDPASNIGGWQWAASTGTDAVPYFRIFNPTTQSQKFDSTGKF
IRKYVKELANLPDKYIHQPEKMSETEQKEHGLLLGKDYPFPIVDHKERRKLAIARYEFSKEHSRGNI
>Mature_466_residues
TSVMWFRRDLRVNDNKALYHACKEDDLLLLFQVNPAQFITGSPSHQAFFASVAHFKQEIDKTAHLQIMFGEPIECFQQLK
DSLPSWDKVYFNRDETGYGAKRDEAAQAFFDEQKIEVQAFHDSYLHSAEEVKKSPTEYYKIFTPYYKKWREEIKETPFKV
TLKHENIRKESLFLKYEEQFAEMTSDLPILDIGERAANTRLANFIKHDVADYDKARDFPELDKTSHLSRYLRTGEISIRT
IWQTLQETEATEGRATFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNREYFKAWQEGKTGFPLVDAAMRQLKE
TGWMHNRLRMITASFLTKDLLIDWRFGEKYFQQMLIDYDPASNIGGWQWAASTGTDAVPYFRIFNPTTQSQKFDSTGKFI
RKYVKELANLPDKYIHQPEKMSETEQKEHGLLLGKDYPFPIVDHKERRKLAIARYEFSKEHSRGNI

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=488, Percent_Identity=27.8688524590164, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI4758072, Length=491, Percent_Identity=28.3095723014257, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI188536103, Length=421, Percent_Identity=28.2660332541568, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1786926, Length=482, Percent_Identity=38.5892116182573, Blast_Score=307, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6324962, Length=376, Percent_Identity=34.8404255319149, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137248, Length=505, Percent_Identity=28.9108910891089, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24585455, Length=505, Percent_Identity=28.9108910891089, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24648152, Length=517, Percent_Identity=24.7582205029014, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55459; Mature: 55328

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVMWFRRDLRVNDNKALYHACKEDDLLLLFQVNPAQFITGSPSHQAFFASVAHFKQEI
CCCCHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECCHHEECCCCCHHHHHHHHHHHHHHH
DKTAHLQIMFGEPIECFQQLKDSLPSWDKVYFNRDETGYGAKRDEAAQAFFDEQKIEVQA
CCCEEEEEEECCHHHHHHHHHHHCCCCHHEEECCCCCCCCCCHHHHHHHHHCCHHEEEEE
FHDSYLHSAEEVKKSPTEYYKIFTPYYKKWREEIKETPFKVTLKHENIRKESLFLKYEEQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
FAEMTSDLPILDIGERAANTRLANFIKHDVADYDKARDFPELDKTSHLSRYLRTGEISIR
HHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHH
TIWQTLQETEATEGRATFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNREYFK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHCCEEEEECCCHHHHH
AWQEGKTGFPLVDAAMRQLKETGWMHNRLRMITASFLTKDLLIDWRFGEKYFQQMLIDYD
HHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHCCC
PASNIGGWQWAASTGTDAVPYFRIFNPTTQSQKFDSTGKFIRKYVKELANLPDKYIHQPE
CCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCH
KMSETEQKEHGLLLGKDYPFPIVDHKERRKLAIARYEFSKEHSRGNI
HHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSVMWFRRDLRVNDNKALYHACKEDDLLLLFQVNPAQFITGSPSHQAFFASVAHFKQEI
CCCHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECCHHEECCCCCHHHHHHHHHHHHHHH
DKTAHLQIMFGEPIECFQQLKDSLPSWDKVYFNRDETGYGAKRDEAAQAFFDEQKIEVQA
CCCEEEEEEECCHHHHHHHHHHHCCCCHHEEECCCCCCCCCCHHHHHHHHHCCHHEEEEE
FHDSYLHSAEEVKKSPTEYYKIFTPYYKKWREEIKETPFKVTLKHENIRKESLFLKYEEQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
FAEMTSDLPILDIGERAANTRLANFIKHDVADYDKARDFPELDKTSHLSRYLRTGEISIR
HHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHH
TIWQTLQETEATEGRATFEKELCWRDFYNMIYVSFPNQKNEPIQENYRFIEWENNREYFK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHCCEEEEECCCHHHHH
AWQEGKTGFPLVDAAMRQLKETGWMHNRLRMITASFLTKDLLIDWRFGEKYFQQMLIDYD
HHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHCCC
PASNIGGWQWAASTGTDAVPYFRIFNPTTQSQKFDSTGKFIRKYVKELANLPDKYIHQPE
CCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCH
KMSETEQKEHGLLLGKDYPFPIVDHKERRKLAIARYEFSKEHSRGNI
HHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678007 [H]