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The map label for this gene is gmuD [H]

Identifier: 226223203

GI number: 226223203

Start: 614888

End: 616282

Strand: Direct

Name: gmuD [H]

Synonym: Lm4b_00600

Alternate gene names: 226223203

Gene position: 614888-616282 (Clockwise)

Preceding gene: 226223201

Following gene: 226223204

Centisome position: 21.11

GC content: 38.71

Gene sequence:

>1395_bases
ATGACTTATAAATTTCCAGAGAATTTTTGGTGGGGAAGTGCGGCGTCCGGCCCACAAACAGAAGGCGCAGCAAATGTAGA
TGGCAGAAAACCGAGTATTTGGGATCACTGGTACAAAATCGAACCAGGTCGTTTCTTTAACGATGTAGGACCTACGAATA
CATCTAATTTTTACTATCAATATAAAGAAGATATTGCTTTAATGAAACAAACTGGGCATAATTCTTTCCGTACTTCGATT
CAGTGGTCACGTCTTATTCCAGATGGTATTGGCGAAGTGAATCCGAAAGCGGTTGATTTTTACAATCGCGTTATTGATGA
AATGCTTGCAAATGACGTGGAACCATTTATGAATTTATATCATTTTGATATGCCAATGTCGATGCAAGAAAAAGGCGGTT
TTGAAAGCCGTGAAGTAGTTGACGCGTATGCAACTTTTGCGAAAACATGTTTTGAATTATTCGGAGATCGTGTGAAACAT
TGGTTTACTTTTAATGAACCAATTGTTCCTGTTGAAGCAGGTTACTTATACGATATGCACTATCCGAATGTCGTTGATTT
TAAACGTGCGACTCAGGTTGCTTACCATACTACTTTGGCACATGCGCTTGCTGTTAAAGAATTCCACGCACTTGAAATTC
CAGAAGGCCAAATTGGAATCATTTTAAACTTAACACCGTCGTATCCAAGAAGCCAAAATCCGGCAGATTTAAAAGCGGCT
CATATTGCAGATCTAATTTTCAACCGTAGTTTCCTTGATCCGGTTACAAAAGGGGAATTCCCAGCTGACCTTGTTGAAAT
TATTCGCGAACATGACGCGCTTCCAGAATATACTGAAGAAGATTTGGCGATAATTAAAAACAACATTATTGATATTCTTG
GTGTAAACTACTACCAACCGCGTCGTGTGAAAGCGAAAGAATACGCAGCTCATCCGGATGCACCATTTATGCCAGAACAT
CTTTTTGATAACTATGAAATGCCTTACCGCAAAATGAATCCATATCGTGGTTGGGAAATTTTCGAAAGAGCAATTTATGA
TATTGCGATTAATCTGCGTGATAACTACGATAATATTCCATTCTTCATTTCTGAAAATGGTATGGGTGTAGAAGGCGAAA
GTCGCTACCGTAATGCAGACGGAATGATTGAAGATACGTACCGAATCGATTTCATTAAGAGTCACTTGAAATGGCTGCAT
AAAGCCATTGAAGAAGGTTCTAACTGTCATGGTTATCATCTTTGGACATTCATGGATTGCTGGAGCTGGGCGAATGCTTA
CAAAAACCGTTATGGCTTAGTAGAAGTTGATTTAGACAATAATTTCAAACGTACTATAAAAGCATCCGGACATTGGTACA
AAGAACTTTCAGAAAACAATGGTTTTGAAGACTAA

Upstream 100 bases:

>100_bases
TGTAAGAAATAAAAATAAAAAACGAACTTTTAAATAAATAAGTTTATACTTTTAAATAAATAAGGTATAATAAAAGCAAA
TAATAGTTGGAGGTAGAAAA

Downstream 100 bases:

>100_bases
ATGTGATAAAATAAAAGTATTATGTGTAAAGAGGTGAACTTCCGTGGCTCAGAACCAGACAAAATATAGCTTTATTGCTG
AAGAAATCCGCAAAAGAATT

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTYKFPENFWWGSAASGPQTEGAANVDGRKPSIWDHWYKIEPGRFFNDVGPTNTSNFYYQYKEDIALMKQTGHNSFRTSI
QWSRLIPDGIGEVNPKAVDFYNRVIDEMLANDVEPFMNLYHFDMPMSMQEKGGFESREVVDAYATFAKTCFELFGDRVKH
WFTFNEPIVPVEAGYLYDMHYPNVVDFKRATQVAYHTTLAHALAVKEFHALEIPEGQIGIILNLTPSYPRSQNPADLKAA
HIADLIFNRSFLDPVTKGEFPADLVEIIREHDALPEYTEEDLAIIKNNIIDILGVNYYQPRRVKAKEYAAHPDAPFMPEH
LFDNYEMPYRKMNPYRGWEIFERAIYDIAINLRDNYDNIPFFISENGMGVEGESRYRNADGMIEDTYRIDFIKSHLKWLH
KAIEEGSNCHGYHLWTFMDCWSWANAYKNRYGLVEVDLDNNFKRTIKASGHWYKELSENNGFED

Sequences:

>Translated_464_residues
MTYKFPENFWWGSAASGPQTEGAANVDGRKPSIWDHWYKIEPGRFFNDVGPTNTSNFYYQYKEDIALMKQTGHNSFRTSI
QWSRLIPDGIGEVNPKAVDFYNRVIDEMLANDVEPFMNLYHFDMPMSMQEKGGFESREVVDAYATFAKTCFELFGDRVKH
WFTFNEPIVPVEAGYLYDMHYPNVVDFKRATQVAYHTTLAHALAVKEFHALEIPEGQIGIILNLTPSYPRSQNPADLKAA
HIADLIFNRSFLDPVTKGEFPADLVEIIREHDALPEYTEEDLAIIKNNIIDILGVNYYQPRRVKAKEYAAHPDAPFMPEH
LFDNYEMPYRKMNPYRGWEIFERAIYDIAINLRDNYDNIPFFISENGMGVEGESRYRNADGMIEDTYRIDFIKSHLKWLH
KAIEEGSNCHGYHLWTFMDCWSWANAYKNRYGLVEVDLDNNFKRTIKASGHWYKELSENNGFED
>Mature_463_residues
TYKFPENFWWGSAASGPQTEGAANVDGRKPSIWDHWYKIEPGRFFNDVGPTNTSNFYYQYKEDIALMKQTGHNSFRTSIQ
WSRLIPDGIGEVNPKAVDFYNRVIDEMLANDVEPFMNLYHFDMPMSMQEKGGFESREVVDAYATFAKTCFELFGDRVKHW
FTFNEPIVPVEAGYLYDMHYPNVVDFKRATQVAYHTTLAHALAVKEFHALEIPEGQIGIILNLTPSYPRSQNPADLKAAH
IADLIFNRSFLDPVTKGEFPADLVEIIREHDALPEYTEEDLAIIKNNIIDILGVNYYQPRRVKAKEYAAHPDAPFMPEHL
FDNYEMPYRKMNPYRGWEIFERAIYDIAINLRDNYDNIPFFISENGMGVEGESRYRNADGMIEDTYRIDFIKSHLKWLHK
AIEEGSNCHGYHLWTFMDCWSWANAYKNRYGLVEVDLDNNFKRTIKASGHWYKELSENNGFED

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=482, Percent_Identity=30.0829875518672, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI13273313, Length=486, Percent_Identity=28.6008230452675, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI32481206, Length=479, Percent_Identity=30.8977035490605, Blast_Score=205, Evalue=8e-53,
Organism=Homo sapiens, GI28376633, Length=476, Percent_Identity=28.1512605042017, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI24497614, Length=481, Percent_Identity=27.6507276507277, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI190360571, Length=97, Percent_Identity=35.0515463917526, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI2367174, Length=488, Percent_Identity=31.9672131147541, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789070, Length=482, Percent_Identity=33.402489626556, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=31.6455696202532, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17539390, Length=471, Percent_Identity=33.7579617834395, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17552856, Length=472, Percent_Identity=31.5677966101695, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21356577, Length=484, Percent_Identity=27.6859504132231, Blast_Score=199, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54116; Mature: 53985

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYKFPENFWWGSAASGPQTEGAANVDGRKPSIWDHWYKIEPGRFFNDVGPTNTSNFYYQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEE
YKEDIALMKQTGHNSFRTSIQWSRLIPDGIGEVNPKAVDFYNRVIDEMLANDVEPFMNLY
EHHHHHHHHHCCCCHHHCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HFDMPMSMQEKGGFESREVVDAYATFAKTCFELFGDRVKHWFTFNEPIVPVEAGYLYDMH
HCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCEEEECC
YPNVVDFKRATQVAYHTTLAHALAVKEFHALEIPEGQIGIILNLTPSYPRSQNPADLKAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCCCCHHHHH
HIADLIFNRSFLDPVTKGEFPADLVEIIREHDALPEYTEEDLAIIKNNIIDILGVNYYQP
HHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHCCCCCCC
RRVKAKEYAAHPDAPFMPEHLFDNYEMPYRKMNPYRGWEIFERAIYDIAINLRDNYDNIP
CCCCHHHHCCCCCCCCCHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHEEEEEECCCCCCC
FFISENGMGVEGESRYRNADGMIEDTYRIDFIKSHLKWLHKAIEEGSNCHGYHLWTFMDC
EEECCCCCCCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
WSWANAYKNRYGLVEVDLDNNFKRTIKASGHWYKELSENNGFED
HHHHHHHHCCCCEEEEECCCCCEEHEECCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TYKFPENFWWGSAASGPQTEGAANVDGRKPSIWDHWYKIEPGRFFNDVGPTNTSNFYYQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEE
YKEDIALMKQTGHNSFRTSIQWSRLIPDGIGEVNPKAVDFYNRVIDEMLANDVEPFMNLY
EHHHHHHHHHCCCCHHHCCEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HFDMPMSMQEKGGFESREVVDAYATFAKTCFELFGDRVKHWFTFNEPIVPVEAGYLYDMH
HCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCEEEECC
YPNVVDFKRATQVAYHTTLAHALAVKEFHALEIPEGQIGIILNLTPSYPRSQNPADLKAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCCCCHHHHH
HIADLIFNRSFLDPVTKGEFPADLVEIIREHDALPEYTEEDLAIIKNNIIDILGVNYYQP
HHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHCCCCCCC
RRVKAKEYAAHPDAPFMPEHLFDNYEMPYRKMNPYRGWEIFERAIYDIAINLRDNYDNIP
CCCCHHHHCCCCCCCCCHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHEEEEEECCCCCCC
FFISENGMGVEGESRYRNADGMIEDTYRIDFIKSHLKWLHKAIEEGSNCHGYHLWTFMDC
EEECCCCCCCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
WSWANAYKNRYGLVEVDLDNNFKRTIKASGHWYKELSENNGFED
HHHHHHHHCCCCEEEEECCCCCEEHEECCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]