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The map label for this gene is prs

Identifier: 226223134

GI number: 226223134

Start: 540073

End: 541008

Strand: Direct

Name: prs

Synonym: Lm4b_00531

Alternate gene names: 226223134

Gene position: 540073-541008 (Clockwise)

Preceding gene: 226223133

Following gene: 226223135

Centisome position: 18.54

GC content: 42.84

Gene sequence:

>936_bases
ATGGCAGGCAAAATGAAACTTTTTTCTGTGACGAGTGAGAGACCACTTGCGACAAAAATTGCAGATTATCTAGATATACC
TTTATGTGAGGTGGAGCTCCAGAAGTTCAGTGATGGAGAAGTGAAAATTAATATTGAGGAAAGTATCCGTGGAACGAATG
CGTATGTTGTGCAATCAATGAATTCCAACGTAAACGAACGTCTAATGGAACTTTTAATCATGGTAGATGCCTTAAAACGC
GCTTCCGTGCATTCCATCAATATAATTATGCCTTATTATGGCTATGCTCGTCAGGACAGAAAAGCTCGCTCAAGAGAACC
AATTACCGCCAAATTAATGGCAAATCTAATTCAAAGAGCTGGAGCCGACCGTTTAATCACAGTAGATTTACACGCAGCTC
AAATTCAAGGCTTTTTCAACATTCCAATCGATCATCTTTCAGCAATTCCGCTAATTGGTGATTATTTGATAGAAAATTAC
GGTGAAAAAGACGTGGTAGTTGTCGCGCCGGATCACAGTGGCGTAGTTCGCGCACGCAGAATCGCGGACCGACTAAACGC
GCCAATCGCCATTTTAAACCGTAAACCAAGACCACATGAAGACGAAATTATGAGTGTCATTGGCGATGTGAAAGGCAAAG
TCGCAATCGTTGTCGATGATATTATCGATACCGGCGTCCGTGCCACTACTTCAGCTGATATTTTACTTGAAAAAGGTGCC
GTAGAAGTTATCGCTTGCGCAACCCACTCCGTCATGGCAGGAAATGCAACCGAACGCTTACAAAATTCCAAAATCAAAGA
AGTCATCACATCGGACTCCATCGATCTTCCAGAAGATAAACAATTCGACAAACTAACAACCATCTCCATCGGACGAATCT
TAGGCCGCGCAATCGAAGGCGTACAAGAAAACCGCTCGTTGCATCCGTTGTTTTAA

Upstream 100 bases:

>100_bases
TTGTTTTATTTGGACCAATGATTTTTAGCTAGTAGACAACACATTGACAGTTCTATATAATAATAAGGAGAAGAAATTAG
TACGGCGGAAGGAGATAGCA

Downstream 100 bases:

>100_bases
AGAATAAGCAAAAAACAGTAACTTAGTAGAGTTGCTGTTTTTTGTTATCTGTCGACCTTGAGTAGCGTGAAAAACACCGG
GAATCGTCAGAAATAGCTCT

Product: phosphoribosyl pyrophosphate synthetase

Products: NA

Alternate protein names: RPPK 2; Phosphoribosyl pyrophosphate synthase 2; P-Rib-PP synthase 2; PRPP synthase 2 [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MAGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKINIEESIRGTNAYVVQSMNSNVNERLMELLIMVDALKR
ASVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRAGADRLITVDLHAAQIQGFFNIPIDHLSAIPLIGDYLIENY
GEKDVVVVAPDHSGVVRARRIADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGA
VEVIACATHSVMAGNATERLQNSKIKEVITSDSIDLPEDKQFDKLTTISIGRILGRAIEGVQENRSLHPLF

Sequences:

>Translated_311_residues
MAGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKINIEESIRGTNAYVVQSMNSNVNERLMELLIMVDALKR
ASVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRAGADRLITVDLHAAQIQGFFNIPIDHLSAIPLIGDYLIENY
GEKDVVVVAPDHSGVVRARRIADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGA
VEVIACATHSVMAGNATERLQNSKIKEVITSDSIDLPEDKQFDKLTTISIGRILGRAIEGVQENRSLHPLF
>Mature_310_residues
AGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKINIEESIRGTNAYVVQSMNSNVNERLMELLIMVDALKRA
SVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRAGADRLITVDLHAAQIQGFFNIPIDHLSAIPLIGDYLIENYG
EKDVVVVAPDHSGVVRARRIADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGAV
EVIACATHSVMAGNATERLQNSKIKEVITSDSIDLPEDKQFDKLTTISIGRILGRAIEGVQENRSLHPLF

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=41.9871794871795, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=42.3076923076923, Blast_Score=241, Evalue=4e-64,
Organism=Homo sapiens, GI4506129, Length=310, Percent_Identity=41.9354838709677, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI84875539, Length=313, Percent_Identity=41.5335463258786, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=33.5260115606936, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI194018537, Length=331, Percent_Identity=32.6283987915408, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI310128524, Length=142, Percent_Identity=33.0985915492958, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310115209, Length=142, Percent_Identity=33.0985915492958, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310118259, Length=142, Percent_Identity=33.0985915492958, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310119946, Length=142, Percent_Identity=33.0985915492958, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=43.9102564102564, Blast_Score=251, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=42.6282051282051, Blast_Score=242, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=42.6282051282051, Blast_Score=241, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=42.5806451612903, Blast_Score=240, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI71989924, Length=312, Percent_Identity=42.6282051282051, Blast_Score=240, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=31.858407079646, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321776, Length=310, Percent_Identity=40.6451612903226, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6320946, Length=310, Percent_Identity=37.4193548387097, Blast_Score=218, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6319403, Length=315, Percent_Identity=38.4126984126984, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6322667, Length=199, Percent_Identity=35.678391959799, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324511, Length=92, Percent_Identity=43.4782608695652, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355239, Length=312, Percent_Identity=41.6666666666667, Blast_Score=237, Evalue=6e-63,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=39.1044776119403, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI281362873, Length=342, Percent_Identity=29.2397660818713, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24651454, Length=342, Percent_Identity=29.2397660818713, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24651458, Length=342, Percent_Identity=29.2397660818713, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24651456, Length=342, Percent_Identity=29.2397660818713, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24651462, Length=179, Percent_Identity=34.6368715083799, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24651464, Length=179, Percent_Identity=34.6368715083799, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI45552010, Length=179, Percent_Identity=34.6368715083799, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34360; Mature: 34229

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKINIEESIRGTNAYVVQSM
CCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEHHCCCCCCEEEEEEC
NSNVNERLMELLIMVDALKRASVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRA
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHC
GADRLITVDLHAAQIQGFFNIPIDHLSAIPLIGDYLIENYGEKDVVVVAPDHSGVVRARR
CCCCEEEEEEEHHHHCCEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
IADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGA
HHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCHHEEEECCC
VEVIACATHSVMAGNATERLQNSKIKEVITSDSIDLPEDKQFDKLTTISIGRILGRAIEG
EEEEEECCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VQENRSLHPLF
HHHCCCCCCCC
>Mature Secondary Structure 
AGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKINIEESIRGTNAYVVQSM
CCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEHHCCCCCCEEEEEEC
NSNVNERLMELLIMVDALKRASVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRA
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHC
GADRLITVDLHAAQIQGFFNIPIDHLSAIPLIGDYLIENYGEKDVVVVAPDHSGVVRARR
CCCCEEEEEEEHHHHCCEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
IADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGA
HHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCHHEEEECCC
VEVIACATHSVMAGNATERLQNSKIKEVITSDSIDLPEDKQFDKLTTISIGRILGRAIEG
EEEEEECCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VQENRSLHPLF
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]