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The map label for this gene is ywbI [H]

Identifier: 226223056

GI number: 226223056

Start: 456036

End: 456911

Strand: Reverse

Name: ywbI [H]

Synonym: Lm4b_00450

Alternate gene names: 226223056

Gene position: 456911-456036 (Counterclockwise)

Preceding gene: 226223067

Following gene: 226223050

Centisome position: 15.69

GC content: 35.27

Gene sequence:

>876_bases
ATGGAGCTTCGTGTGTTGAATTATTTTTTAACTGTTGCACGTGAAAAAACGATTAGTCAAGCGGCGGAAGTGCTTCATCT
TTCTCAGCCAACGCTTTCTAAACAATTGAAGGAACTTGAGGAAGAACTAGGGGTAACTTTATTTATTCGTGGAAATCGGT
CTATTACGTTAACAGAGGATGGGCTTTATTTAGCTAATCGCGGGAAAGAAATTTTATCTTTAGTGGAGTTAACTACTTCG
AATTTACTTCAAAATGAGACGATTAGCGGAAAAATAATGATTGGTGCTGCTGAAACGAGAGGATTTGAATTTATCGGTAG
CATGTTACATGAACTTAGAGAGAAGCATTCCGACATTACTTTTCAATTGCATAGCGGCAATGCGGATGATGTTTTAGAAA
AAATTGAGCACGGTTTACTAGATTTTGGTTTGGTAATTAATCCTGTGGAAAAACTACAATATGAATATTTACCACTTAAT
ATAGAAGATACTTGGGGAATTTTAGTCAATACAAAACATTCTTTAGCGAAAAAAGCGGTTGTAACTCCTAGTGATATTGA
AGCTAATCCGATTGTCGTTTCCAACCAATCTTTCATTGATAATCAGCTAGCTAGTTGGCTTGGAGAGCATTTTGAACAAT
TGAATGTAATTGGTAGATATAATTTACTGTATAATGCCTCTTTGCTCGCTAAGGAGAACATCGCAAGTATTCTTTGTATT
GATGGTATTATCAATACGGAAAATACTAACCTGAAGTTTATCCCTTTTTCGCCGCCTCTAATTGCTGGAATTAGTATTGT
GTGGAAGAAGAATCAAACTTTCTCCAGTGCTTCGAAGGAATTTTTACGATTAATACAAAAAACTTGCTCAAAATAA

Upstream 100 bases:

>100_bases
AACCTTTTACTATTTAATTATAGCGGGAGAAGTTATAATTGAATAATACTTATAAAGCATATCCAACTATTCATTTTAGT
TATAGGAAAGGGTGATTTTA

Downstream 100 bases:

>100_bases
ATACTTTGAGCAAGTTTTCTTTCTATATTTCTAATCGTAACGTAACAGCGCCATATGCTGGAATCGTGTTGTTGTCATTT
GCAACAACTTCTTCGATAGC

Product: transcriptional regulator (LysR family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MELRVLNYFLTVAREKTISQAAEVLHLSQPTLSKQLKELEEELGVTLFIRGNRSITLTEDGLYLANRGKEILSLVELTTS
NLLQNETISGKIMIGAAETRGFEFIGSMLHELREKHSDITFQLHSGNADDVLEKIEHGLLDFGLVINPVEKLQYEYLPLN
IEDTWGILVNTKHSLAKKAVVTPSDIEANPIVVSNQSFIDNQLASWLGEHFEQLNVIGRYNLLYNASLLAKENIASILCI
DGIINTENTNLKFIPFSPPLIAGISIVWKKNQTFSSASKEFLRLIQKTCSK

Sequences:

>Translated_291_residues
MELRVLNYFLTVAREKTISQAAEVLHLSQPTLSKQLKELEEELGVTLFIRGNRSITLTEDGLYLANRGKEILSLVELTTS
NLLQNETISGKIMIGAAETRGFEFIGSMLHELREKHSDITFQLHSGNADDVLEKIEHGLLDFGLVINPVEKLQYEYLPLN
IEDTWGILVNTKHSLAKKAVVTPSDIEANPIVVSNQSFIDNQLASWLGEHFEQLNVIGRYNLLYNASLLAKENIASILCI
DGIINTENTNLKFIPFSPPLIAGISIVWKKNQTFSSASKEFLRLIQKTCSK
>Mature_291_residues
MELRVLNYFLTVAREKTISQAAEVLHLSQPTLSKQLKELEEELGVTLFIRGNRSITLTEDGLYLANRGKEILSLVELTTS
NLLQNETISGKIMIGAAETRGFEFIGSMLHELREKHSDITFQLHSGNADDVLEKIEHGLLDFGLVINPVEKLQYEYLPLN
IEDTWGILVNTKHSLAKKAVVTPSDIEANPIVVSNQSFIDNQLASWLGEHFEQLNVIGRYNLLYNASLLAKENIASILCI
DGIINTENTNLKFIPFSPPLIAGISIVWKKNQTFSSASKEFLRLIQKTCSK

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=301, Percent_Identity=25.9136212624585, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788887, Length=297, Percent_Identity=27.6094276094276, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787879, Length=302, Percent_Identity=25.1655629139073, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788748, Length=292, Percent_Identity=28.4246575342466, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790208, Length=217, Percent_Identity=27.6497695852535, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790399, Length=187, Percent_Identity=28.3422459893048, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=23.728813559322, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081904, Length=207, Percent_Identity=28.9855072463768, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788297, Length=163, Percent_Identity=31.9018404907975, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787806, Length=163, Percent_Identity=29.4478527607362, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=135, Percent_Identity=28.1481481481481, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367136, Length=71, Percent_Identity=46.4788732394366, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=207, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32624; Mature: 32624

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRVLNYFLTVAREKTISQAAEVLHLSQPTLSKQLKELEEELGVTLFIRGNRSITLTED
CCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCEEEEECC
GLYLANRGKEILSLVELTTSNLLQNETISGKIMIGAAETRGFEFIGSMLHELREKHSDIT
CEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEE
FQLHSGNADDVLEKIEHGLLDFGLVINPVEKLQYEYLPLNIEDTWGILVNTKHSLAKKAV
EEEECCCHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEEECCCCEEEEECHHHHHHHHC
VTPSDIEANPIVVSNQSFIDNQLASWLGEHFEQLNVIGRYNLLYNASLLAKENIASILCI
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHEEEHHEECCHHHHHHCCCEEEEE
DGIINTENTNLKFIPFSPPLIAGISIVWKKNQTFSSASKEFLRLIQKTCSK
CCEEECCCCCEEEEECCCCHHHCEEEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELRVLNYFLTVAREKTISQAAEVLHLSQPTLSKQLKELEEELGVTLFIRGNRSITLTED
CCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCEEEEECC
GLYLANRGKEILSLVELTTSNLLQNETISGKIMIGAAETRGFEFIGSMLHELREKHSDIT
CEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEE
FQLHSGNADDVLEKIEHGLLDFGLVINPVEKLQYEYLPLNIEDTWGILVNTKHSLAKKAV
EEEECCCHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEEECCCCEEEEECHHHHHHHHC
VTPSDIEANPIVVSNQSFIDNQLASWLGEHFEQLNVIGRYNLLYNASLLAKENIASILCI
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHEEEHHEECCHHHHHHCCCEEEEE
DGIINTENTNLKFIPFSPPLIAGISIVWKKNQTFSSASKEFLRLIQKTCSK
CCEEECCCCCEEEEECCCCHHHCEEEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]